Efeito de fragmentos de peptídeos catiônicos sobre cultura planctônica e biofilme de cepas de S. mutans

• Autor(a) principal: Matias, Luis Mateus de Almeida [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149652; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-30/000867389.pdf
• topic: Cárie dentária; Peptídeos; Testes de sensibilidade microbiana; Dental caries

Dental caries is the most prevalent chronic childhood disease. The bacterial group considered more cariogenic is mutans streptococci (MS), especially Streptococcus mutans, one of the primary bacterial colonizers of dental enamel. Antimicrobial agents could be used to control proliferation of cariogenic bacteria avoiding dental decay. Among these agents, cationic peptides have been demonstrated antimicrobial activity against oral pathogens. The aim of study was to evaluate the antimicrobial activity of fragments obtained from oral cationic peptides: LL-37 (from CAP18), D1-23 and D6-17 (both from HBD-3). In order to evaluate the antimicrobial activity, CP fragments were tested against three Streptococcus mutansstrains (one standard and two clinical strains) and determined minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Biofilm assays were conducted with CHX and the best CP fragment. Antimicrobial tests showed that D1-23 had the best bactericidal activity against S. mutans strains, in planktonic conditions. D1-23 (2x MBC) had similar S. mutansantibiofilm effect compared to CHX. It was concluded that D1-23 had activity against S. mutans strains, in planktonic and biofilm conditions, suggesting their potential as antimicrobial agent for oral application