Desenvolvimento e validação de método de análise de nifedipino em fluidos biológicos por cromatografia líquida de alta eficiência

• Autor(a) principal: Borsari, Bruno [UNESP]
• Data de Publicação: 2017
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/204023; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2019-05-20/000914171.pdf
• topic: Nifedipina; Plasma; Leite humano; Lactente; Cromatografia líquida de alta eficiência; Breast milk

Nifedipine is an antihypertensive of the calcium channel blocker type accepted for use during gestation and lactation. Studies have shown that nifedipine is transferred into breast milk through passive diffusion and active transport mediated by ABCG2 / BCRP. Chromatographic methods for an investigation of the factors influencing the transfer of nifedipine to breast milk are necessary and to this moment there is no description of the method of analysis of nifedipine in breast milk. The objective of the present study was to develop and validate methods of nifedipine analysis in human plasma and human milk samples by high performance liquid chromatography. Plasma concentrations of nifedipine were assessed by high performance liquid chromatography with ultraviolet detection (HPLC-UV). The method of analysis of nifedipine by HPLC-UV showed linearity in the range of 10 to 150 ng/mL for plasma and 5 to 100 ng/mL for breast milk. The sensitivity of the method was compatible with a desired application with quantification limits of 10 ng/mL and 5 ng/mL for plasma and breast milk, respectively. The precision, evaluated by coefficient of variation, and the accuracy, evaluated by the relative error, was lower than 15%. The methods were used to investigate the transfer of nifedipine to breast milk in hypertensive lactants treated with controlled release tablets at a dose of 20 mg every 12 hours attended at the High Risk Pregnancy Outpatient Clinic of Ribeirão Preto Medical School, Univesity of São Paulo.