miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Bragato, Jaqueline Poleto [UNESP]
Data de Publicação: 2022
Outros Autores: Rebech, Gabriela Torres [UNESP], de Freitas, Jéssica Henrique [UNESP], dos Santos, Marilene Oliveira [UNESP], Costa, Sidnei Ferro [UNESP], de Rezende Eugênio, Flavia [UNESP], dos Santos, Paulo Sérgio Patto [UNESP], de Lima, Valéria Marçal Felix [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0265192
http://hdl.handle.net/11449/223707
Resumo: Visceral leishmaniasis in humans is a chronic and fatal disease if left untreated. Canine leishmaniasis (CanL) is a severe public health problem because infected animals are powerful transmitters of the parasite to humans via phlebotomine vectors. Therefore, dogs are an essential target for control measures. Progression of canine infection is accompanied by failure of cellular immunity with reduction of circulating lymphocytes and increased cytokines that suppress macrophage function. Studies showed that the regulation of the effector function of macrophages and T cells appears to depend on miRNAs; miRNA-21 (miR-21) shows increased expression in splenic leukocytes of dogs with CanL and targets genes related to the immune response. Mimics and inhibitors of miR-21 were used in vitro to transfect splenic leukocytes from dogs with CanL. After transfection, expression levels of the proteins FAS, FASL, CD69, CCR7, TNF-α, IL-17, IFN-γ, and IL-10 were measured. FAS, FASL, CD69, and CCR7 expression levels decreased in splenic leukocytes from dogs with CanL. The miR-21 mimic decreased CD69 expression in splenic leukocytes from CanL and healthy groups. The miR-21 inhibitor decreased IL-10 levels in culture supernatants from splenic leukocytes in the CanL group. These findings suggest that miR-21 alters the immune response in CanL; therefore, miR-21 could be used as a possible therapeutic target for CanL.
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spelling miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasisVisceral leishmaniasis in humans is a chronic and fatal disease if left untreated. Canine leishmaniasis (CanL) is a severe public health problem because infected animals are powerful transmitters of the parasite to humans via phlebotomine vectors. Therefore, dogs are an essential target for control measures. Progression of canine infection is accompanied by failure of cellular immunity with reduction of circulating lymphocytes and increased cytokines that suppress macrophage function. Studies showed that the regulation of the effector function of macrophages and T cells appears to depend on miRNAs; miRNA-21 (miR-21) shows increased expression in splenic leukocytes of dogs with CanL and targets genes related to the immune response. Mimics and inhibitors of miR-21 were used in vitro to transfect splenic leukocytes from dogs with CanL. After transfection, expression levels of the proteins FAS, FASL, CD69, CCR7, TNF-α, IL-17, IFN-γ, and IL-10 were measured. FAS, FASL, CD69, and CCR7 expression levels decreased in splenic leukocytes from dogs with CanL. The miR-21 mimic decreased CD69 expression in splenic leukocytes from CanL and healthy groups. The miR-21 inhibitor decreased IL-10 levels in culture supernatants from splenic leukocytes in the CanL group. These findings suggest that miR-21 alters the immune response in CanL; therefore, miR-21 could be used as a possible therapeutic target for CanL.Department of Animal Clinic Surgery and Reproduction São Paulo State University School of Veterinary Medicine, São PauloDepartment of Animal Clinic Surgery and Reproduction São Paulo State University School of Veterinary Medicine, São PauloUniversidade Estadual Paulista (UNESP)Bragato, Jaqueline Poleto [UNESP]Rebech, Gabriela Torres [UNESP]de Freitas, Jéssica Henrique [UNESP]dos Santos, Marilene Oliveira [UNESP]Costa, Sidnei Ferro [UNESP]de Rezende Eugênio, Flavia [UNESP]dos Santos, Paulo Sérgio Patto [UNESP]de Lima, Valéria Marçal Felix [UNESP]2022-04-28T19:52:39Z2022-04-28T19:52:39Z2022-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0265192PLoS ONE, v. 17, n. 3 March, 2022.1932-6203http://hdl.handle.net/11449/22370710.1371/journal.pone.02651922-s2.0-85126910031Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2022-04-28T19:52:39Zoai:repositorio.unesp.br:11449/223707Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:38:42.415045Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis
title miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis
spellingShingle miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis
Bragato, Jaqueline Poleto [UNESP]
title_short miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis
title_full miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis
title_fullStr miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis
title_full_unstemmed miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis
title_sort miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis
author Bragato, Jaqueline Poleto [UNESP]
author_facet Bragato, Jaqueline Poleto [UNESP]
Rebech, Gabriela Torres [UNESP]
de Freitas, Jéssica Henrique [UNESP]
dos Santos, Marilene Oliveira [UNESP]
Costa, Sidnei Ferro [UNESP]
de Rezende Eugênio, Flavia [UNESP]
dos Santos, Paulo Sérgio Patto [UNESP]
de Lima, Valéria Marçal Felix [UNESP]
author_role author
author2 Rebech, Gabriela Torres [UNESP]
de Freitas, Jéssica Henrique [UNESP]
dos Santos, Marilene Oliveira [UNESP]
Costa, Sidnei Ferro [UNESP]
de Rezende Eugênio, Flavia [UNESP]
dos Santos, Paulo Sérgio Patto [UNESP]
de Lima, Valéria Marçal Felix [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Bragato, Jaqueline Poleto [UNESP]
Rebech, Gabriela Torres [UNESP]
de Freitas, Jéssica Henrique [UNESP]
dos Santos, Marilene Oliveira [UNESP]
Costa, Sidnei Ferro [UNESP]
de Rezende Eugênio, Flavia [UNESP]
dos Santos, Paulo Sérgio Patto [UNESP]
de Lima, Valéria Marçal Felix [UNESP]
description Visceral leishmaniasis in humans is a chronic and fatal disease if left untreated. Canine leishmaniasis (CanL) is a severe public health problem because infected animals are powerful transmitters of the parasite to humans via phlebotomine vectors. Therefore, dogs are an essential target for control measures. Progression of canine infection is accompanied by failure of cellular immunity with reduction of circulating lymphocytes and increased cytokines that suppress macrophage function. Studies showed that the regulation of the effector function of macrophages and T cells appears to depend on miRNAs; miRNA-21 (miR-21) shows increased expression in splenic leukocytes of dogs with CanL and targets genes related to the immune response. Mimics and inhibitors of miR-21 were used in vitro to transfect splenic leukocytes from dogs with CanL. After transfection, expression levels of the proteins FAS, FASL, CD69, CCR7, TNF-α, IL-17, IFN-γ, and IL-10 were measured. FAS, FASL, CD69, and CCR7 expression levels decreased in splenic leukocytes from dogs with CanL. The miR-21 mimic decreased CD69 expression in splenic leukocytes from CanL and healthy groups. The miR-21 inhibitor decreased IL-10 levels in culture supernatants from splenic leukocytes in the CanL group. These findings suggest that miR-21 alters the immune response in CanL; therefore, miR-21 could be used as a possible therapeutic target for CanL.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-28T19:52:39Z
2022-04-28T19:52:39Z
2022-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0265192
PLoS ONE, v. 17, n. 3 March, 2022.
1932-6203
http://hdl.handle.net/11449/223707
10.1371/journal.pone.0265192
2-s2.0-85126910031
url http://dx.doi.org/10.1371/journal.pone.0265192
http://hdl.handle.net/11449/223707
identifier_str_mv PLoS ONE, v. 17, n. 3 March, 2022.
1932-6203
10.1371/journal.pone.0265192
2-s2.0-85126910031
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS ONE
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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