miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0265192 http://hdl.handle.net/11449/223707 |
Resumo: | Visceral leishmaniasis in humans is a chronic and fatal disease if left untreated. Canine leishmaniasis (CanL) is a severe public health problem because infected animals are powerful transmitters of the parasite to humans via phlebotomine vectors. Therefore, dogs are an essential target for control measures. Progression of canine infection is accompanied by failure of cellular immunity with reduction of circulating lymphocytes and increased cytokines that suppress macrophage function. Studies showed that the regulation of the effector function of macrophages and T cells appears to depend on miRNAs; miRNA-21 (miR-21) shows increased expression in splenic leukocytes of dogs with CanL and targets genes related to the immune response. Mimics and inhibitors of miR-21 were used in vitro to transfect splenic leukocytes from dogs with CanL. After transfection, expression levels of the proteins FAS, FASL, CD69, CCR7, TNF-α, IL-17, IFN-γ, and IL-10 were measured. FAS, FASL, CD69, and CCR7 expression levels decreased in splenic leukocytes from dogs with CanL. The miR-21 mimic decreased CD69 expression in splenic leukocytes from CanL and healthy groups. The miR-21 inhibitor decreased IL-10 levels in culture supernatants from splenic leukocytes in the CanL group. These findings suggest that miR-21 alters the immune response in CanL; therefore, miR-21 could be used as a possible therapeutic target for CanL. |
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miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasisVisceral leishmaniasis in humans is a chronic and fatal disease if left untreated. Canine leishmaniasis (CanL) is a severe public health problem because infected animals are powerful transmitters of the parasite to humans via phlebotomine vectors. Therefore, dogs are an essential target for control measures. Progression of canine infection is accompanied by failure of cellular immunity with reduction of circulating lymphocytes and increased cytokines that suppress macrophage function. Studies showed that the regulation of the effector function of macrophages and T cells appears to depend on miRNAs; miRNA-21 (miR-21) shows increased expression in splenic leukocytes of dogs with CanL and targets genes related to the immune response. Mimics and inhibitors of miR-21 were used in vitro to transfect splenic leukocytes from dogs with CanL. After transfection, expression levels of the proteins FAS, FASL, CD69, CCR7, TNF-α, IL-17, IFN-γ, and IL-10 were measured. FAS, FASL, CD69, and CCR7 expression levels decreased in splenic leukocytes from dogs with CanL. The miR-21 mimic decreased CD69 expression in splenic leukocytes from CanL and healthy groups. The miR-21 inhibitor decreased IL-10 levels in culture supernatants from splenic leukocytes in the CanL group. These findings suggest that miR-21 alters the immune response in CanL; therefore, miR-21 could be used as a possible therapeutic target for CanL.Department of Animal Clinic Surgery and Reproduction São Paulo State University School of Veterinary Medicine, São PauloDepartment of Animal Clinic Surgery and Reproduction São Paulo State University School of Veterinary Medicine, São PauloUniversidade Estadual Paulista (UNESP)Bragato, Jaqueline Poleto [UNESP]Rebech, Gabriela Torres [UNESP]de Freitas, Jéssica Henrique [UNESP]dos Santos, Marilene Oliveira [UNESP]Costa, Sidnei Ferro [UNESP]de Rezende Eugênio, Flavia [UNESP]dos Santos, Paulo Sérgio Patto [UNESP]de Lima, Valéria Marçal Felix [UNESP]2022-04-28T19:52:39Z2022-04-28T19:52:39Z2022-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0265192PLoS ONE, v. 17, n. 3 March, 2022.1932-6203http://hdl.handle.net/11449/22370710.1371/journal.pone.02651922-s2.0-85126910031Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2022-04-28T19:52:39Zoai:repositorio.unesp.br:11449/223707Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:38:42.415045Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis |
title |
miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis |
spellingShingle |
miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis Bragato, Jaqueline Poleto [UNESP] |
title_short |
miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis |
title_full |
miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis |
title_fullStr |
miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis |
title_full_unstemmed |
miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis |
title_sort |
miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis |
author |
Bragato, Jaqueline Poleto [UNESP] |
author_facet |
Bragato, Jaqueline Poleto [UNESP] Rebech, Gabriela Torres [UNESP] de Freitas, Jéssica Henrique [UNESP] dos Santos, Marilene Oliveira [UNESP] Costa, Sidnei Ferro [UNESP] de Rezende Eugênio, Flavia [UNESP] dos Santos, Paulo Sérgio Patto [UNESP] de Lima, Valéria Marçal Felix [UNESP] |
author_role |
author |
author2 |
Rebech, Gabriela Torres [UNESP] de Freitas, Jéssica Henrique [UNESP] dos Santos, Marilene Oliveira [UNESP] Costa, Sidnei Ferro [UNESP] de Rezende Eugênio, Flavia [UNESP] dos Santos, Paulo Sérgio Patto [UNESP] de Lima, Valéria Marçal Felix [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Bragato, Jaqueline Poleto [UNESP] Rebech, Gabriela Torres [UNESP] de Freitas, Jéssica Henrique [UNESP] dos Santos, Marilene Oliveira [UNESP] Costa, Sidnei Ferro [UNESP] de Rezende Eugênio, Flavia [UNESP] dos Santos, Paulo Sérgio Patto [UNESP] de Lima, Valéria Marçal Felix [UNESP] |
description |
Visceral leishmaniasis in humans is a chronic and fatal disease if left untreated. Canine leishmaniasis (CanL) is a severe public health problem because infected animals are powerful transmitters of the parasite to humans via phlebotomine vectors. Therefore, dogs are an essential target for control measures. Progression of canine infection is accompanied by failure of cellular immunity with reduction of circulating lymphocytes and increased cytokines that suppress macrophage function. Studies showed that the regulation of the effector function of macrophages and T cells appears to depend on miRNAs; miRNA-21 (miR-21) shows increased expression in splenic leukocytes of dogs with CanL and targets genes related to the immune response. Mimics and inhibitors of miR-21 were used in vitro to transfect splenic leukocytes from dogs with CanL. After transfection, expression levels of the proteins FAS, FASL, CD69, CCR7, TNF-α, IL-17, IFN-γ, and IL-10 were measured. FAS, FASL, CD69, and CCR7 expression levels decreased in splenic leukocytes from dogs with CanL. The miR-21 mimic decreased CD69 expression in splenic leukocytes from CanL and healthy groups. The miR-21 inhibitor decreased IL-10 levels in culture supernatants from splenic leukocytes in the CanL group. These findings suggest that miR-21 alters the immune response in CanL; therefore, miR-21 could be used as a possible therapeutic target for CanL. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:52:39Z 2022-04-28T19:52:39Z 2022-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0265192 PLoS ONE, v. 17, n. 3 March, 2022. 1932-6203 http://hdl.handle.net/11449/223707 10.1371/journal.pone.0265192 2-s2.0-85126910031 |
url |
http://dx.doi.org/10.1371/journal.pone.0265192 http://hdl.handle.net/11449/223707 |
identifier_str_mv |
PLoS ONE, v. 17, n. 3 March, 2022. 1932-6203 10.1371/journal.pone.0265192 2-s2.0-85126910031 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129446020907008 |