Blood flow distribution in embryonic common snapping turtles Chelydra serpentina (Reptilia; Chelonia) during acute hypoxia and alpha-adrenergic regulation

Detalhes bibliográficos
Autor(a) principal: Sartori, Marina R. [UNESP]
Data de Publicação: 2019
Outros Autores: Kohl, Zachary F., Taylor, Edwin W. [UNESP], Abe, Augusto S. [UNESP], Crossley, Dane A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.cbpa.2019.110575
http://hdl.handle.net/11449/196332
Resumo: Embryonic turtles have four distinct vascular beds that separately perfuse the developing embryo's body and the extra-embryonic yolk sac, amnion and chorioallantoic membrane (CAM). The mechanisms enabling differential regulation of blood flow through these separate beds, in order to meet the varying demands of the embryo during development, is of current interest. The present investigation followed the changes in blood flow distribution during an acute exposure to hypoxia and after alpha-adrenergic blockade. We monitored heart rate (f(H)), mean arterial pressure (P-m), and determined relative blood flow distribution (%(Q) over dot sys) using colored microspheres. At 70% and 90% of the incubation period hypoxia elicited a bradycardia without changing P-m while %(Q) over dot sys was altered only at 70%, increasing to the CAM and liver. Blockade of alpha-adrenergic responses with phentolamine did not change f(H) or P-m but increased %(Q) over dot sys to the shell. These results show the capacity of embryos to redistribute cardiac output during acute hypoxia, however a-adrenergic receptors seemed to play a relatively small role in embryonic cardiovascular regulation.
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spelling Blood flow distribution in embryonic common snapping turtles Chelydra serpentina (Reptilia; Chelonia) during acute hypoxia and alpha-adrenergic regulationCardiovascularCardiac outputReptilesMicrospheresEmbryonic turtles have four distinct vascular beds that separately perfuse the developing embryo's body and the extra-embryonic yolk sac, amnion and chorioallantoic membrane (CAM). The mechanisms enabling differential regulation of blood flow through these separate beds, in order to meet the varying demands of the embryo during development, is of current interest. The present investigation followed the changes in blood flow distribution during an acute exposure to hypoxia and after alpha-adrenergic blockade. We monitored heart rate (f(H)), mean arterial pressure (P-m), and determined relative blood flow distribution (%(Q) over dot sys) using colored microspheres. At 70% and 90% of the incubation period hypoxia elicited a bradycardia without changing P-m while %(Q) over dot sys was altered only at 70%, increasing to the CAM and liver. Blockade of alpha-adrenergic responses with phentolamine did not change f(H) or P-m but increased %(Q) over dot sys to the shell. These results show the capacity of embryos to redistribute cardiac output during acute hypoxia, however a-adrenergic receptors seemed to play a relatively small role in embryonic cardiovascular regulation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)National Science FoundationUniv Estadual Paulista, Inst Biociencias, Dept Zool, Campus Rio Claro, BR-13506900 Rio Claro, SP, BrazilUniv North Texas, Dept Biol Sci, Dev Integrat Biol Cluster, Denton, TX 76203 USAUniv Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, EnglandUniv Estadual Paulista, Inst Biociencias, Dept Zool, Campus Rio Claro, BR-13506900 Rio Claro, SP, BrazilFAPESP: 2014/01666-5FAPESP: 2012/06938-8CNPq: 401061/2014-0National Science Foundation: NSF IBN-IOS 0845741Elsevier B.V.Universidade Estadual Paulista (Unesp)Univ North TexasUniv BirminghamSartori, Marina R. [UNESP]Kohl, Zachary F.Taylor, Edwin W. [UNESP]Abe, Augusto S. [UNESP]Crossley, Dane A.2020-12-10T19:41:12Z2020-12-10T19:41:12Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5http://dx.doi.org/10.1016/j.cbpa.2019.110575Comparative Biochemistry And Physiology A-molecular & Integrative Physiology. New York: Elsevier Science Inc, v. 238, 5 p., 2019.1095-6433http://hdl.handle.net/11449/19633210.1016/j.cbpa.2019.110575WOS:000496875400009Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Biochemistry And Physiology A-molecular & Integrative Physiologyinfo:eu-repo/semantics/openAccess2021-10-23T07:07:38Zoai:repositorio.unesp.br:11449/196332Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:33:56.257103Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Blood flow distribution in embryonic common snapping turtles Chelydra serpentina (Reptilia; Chelonia) during acute hypoxia and alpha-adrenergic regulation
title Blood flow distribution in embryonic common snapping turtles Chelydra serpentina (Reptilia; Chelonia) during acute hypoxia and alpha-adrenergic regulation
spellingShingle Blood flow distribution in embryonic common snapping turtles Chelydra serpentina (Reptilia; Chelonia) during acute hypoxia and alpha-adrenergic regulation
Sartori, Marina R. [UNESP]
Cardiovascular
Cardiac output
Reptiles
Microspheres
title_short Blood flow distribution in embryonic common snapping turtles Chelydra serpentina (Reptilia; Chelonia) during acute hypoxia and alpha-adrenergic regulation
title_full Blood flow distribution in embryonic common snapping turtles Chelydra serpentina (Reptilia; Chelonia) during acute hypoxia and alpha-adrenergic regulation
title_fullStr Blood flow distribution in embryonic common snapping turtles Chelydra serpentina (Reptilia; Chelonia) during acute hypoxia and alpha-adrenergic regulation
title_full_unstemmed Blood flow distribution in embryonic common snapping turtles Chelydra serpentina (Reptilia; Chelonia) during acute hypoxia and alpha-adrenergic regulation
title_sort Blood flow distribution in embryonic common snapping turtles Chelydra serpentina (Reptilia; Chelonia) during acute hypoxia and alpha-adrenergic regulation
author Sartori, Marina R. [UNESP]
author_facet Sartori, Marina R. [UNESP]
Kohl, Zachary F.
Taylor, Edwin W. [UNESP]
Abe, Augusto S. [UNESP]
Crossley, Dane A.
author_role author
author2 Kohl, Zachary F.
Taylor, Edwin W. [UNESP]
Abe, Augusto S. [UNESP]
Crossley, Dane A.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ North Texas
Univ Birmingham
dc.contributor.author.fl_str_mv Sartori, Marina R. [UNESP]
Kohl, Zachary F.
Taylor, Edwin W. [UNESP]
Abe, Augusto S. [UNESP]
Crossley, Dane A.
dc.subject.por.fl_str_mv Cardiovascular
Cardiac output
Reptiles
Microspheres
topic Cardiovascular
Cardiac output
Reptiles
Microspheres
description Embryonic turtles have four distinct vascular beds that separately perfuse the developing embryo's body and the extra-embryonic yolk sac, amnion and chorioallantoic membrane (CAM). The mechanisms enabling differential regulation of blood flow through these separate beds, in order to meet the varying demands of the embryo during development, is of current interest. The present investigation followed the changes in blood flow distribution during an acute exposure to hypoxia and after alpha-adrenergic blockade. We monitored heart rate (f(H)), mean arterial pressure (P-m), and determined relative blood flow distribution (%(Q) over dot sys) using colored microspheres. At 70% and 90% of the incubation period hypoxia elicited a bradycardia without changing P-m while %(Q) over dot sys was altered only at 70%, increasing to the CAM and liver. Blockade of alpha-adrenergic responses with phentolamine did not change f(H) or P-m but increased %(Q) over dot sys to the shell. These results show the capacity of embryos to redistribute cardiac output during acute hypoxia, however a-adrenergic receptors seemed to play a relatively small role in embryonic cardiovascular regulation.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-01
2020-12-10T19:41:12Z
2020-12-10T19:41:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.cbpa.2019.110575
Comparative Biochemistry And Physiology A-molecular & Integrative Physiology. New York: Elsevier Science Inc, v. 238, 5 p., 2019.
1095-6433
http://hdl.handle.net/11449/196332
10.1016/j.cbpa.2019.110575
WOS:000496875400009
url http://dx.doi.org/10.1016/j.cbpa.2019.110575
http://hdl.handle.net/11449/196332
identifier_str_mv Comparative Biochemistry And Physiology A-molecular & Integrative Physiology. New York: Elsevier Science Inc, v. 238, 5 p., 2019.
1095-6433
10.1016/j.cbpa.2019.110575
WOS:000496875400009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Comparative Biochemistry And Physiology A-molecular & Integrative Physiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 5
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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