Transposon Tn1721 distribution among strains of Xylella fastidiosa

Detalhes bibliográficos
Autor(a) principal: Ferreira, Lucia P [UNESP]
Data de Publicação: 2002
Outros Autores: Lemos, Eliana G.M [UNESP], Lemos, Manoel Victor F [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/S0378-1097(01)00473-6
http://hdl.handle.net/11449/66849
Resumo: Transposons are mobile genetic elements found within the genomes of various organisms including bacteria, fungi, plants and animals. Fragments of the transposon Tn1721 were found included in the genome of Xylella fastidiosa strain 9a5c. Regions from such fragments were PCR-amplified using specially designed primers (TNP1 and TNP2). In order to detect insertions of the Tn1721 element, both primers were used and one of them included a region of the transposon (TNP1) and the other one had the right repeat and part of the bacterial chromosome (TNP2). The PCR products obtained from strain 9a5c were used as a pattern for fragment size comparisons when DNA samples from other X. fastidiosa strains were used as template for the PCR assays. Differences were observed concerning the PCR products of such amplifications when some X. fastidiosa strains isolated from grapevine and plum were used. For the citrus-derived strains only the strains U187d and GP920b produced fragments with different sizes or weak band intensity. Such variations in the X. fastidiosa genome related to disrupted Tn1721 copies are probably due to the possibility of such a transposon element being still able to duplicate even after deletion events might have taken place and also because the bacterial strains in which the main differences were detected are derived from different host plants cultivated under different climate conditions from the one used as reference. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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spelling Transposon Tn1721 distribution among strains of Xylella fastidiosaPCR fragmentTransposon Tn1721Xylella fastidiosabacterial DNAbacterial chromosomebacterial genomebacterial strainbacterium isolationcitrus fruitcontrolled studyDNA templategene amplificationgene deletiongene duplicationgenetic variabilityGram negative bacteriumnonhumanplumpolymerase chain reactionpriority journaltransposonxylell fastidiosaBase SequenceCitrusCoffeeDNA Transposable ElementsDNA, BacterialGammaproteobacteriaMolecular Sequence DataPlant DiseasesPrunusSequence AlignmentSpecies SpecificityAnimaliaBacteria (microorganisms)FungiPrunus domesticatransposonsVitisTransposons are mobile genetic elements found within the genomes of various organisms including bacteria, fungi, plants and animals. Fragments of the transposon Tn1721 were found included in the genome of Xylella fastidiosa strain 9a5c. Regions from such fragments were PCR-amplified using specially designed primers (TNP1 and TNP2). In order to detect insertions of the Tn1721 element, both primers were used and one of them included a region of the transposon (TNP1) and the other one had the right repeat and part of the bacterial chromosome (TNP2). The PCR products obtained from strain 9a5c were used as a pattern for fragment size comparisons when DNA samples from other X. fastidiosa strains were used as template for the PCR assays. Differences were observed concerning the PCR products of such amplifications when some X. fastidiosa strains isolated from grapevine and plum were used. For the citrus-derived strains only the strains U187d and GP920b produced fragments with different sizes or weak band intensity. Such variations in the X. fastidiosa genome related to disrupted Tn1721 copies are probably due to the possibility of such a transposon element being still able to duplicate even after deletion events might have taken place and also because the bacterial strains in which the main differences were detected are derived from different host plants cultivated under different climate conditions from the one used as reference. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.Departmento de Biologia Aplicada À Agropecuária FCAV UNESP, Via de Acesso Prof Paulo D.C. s/n, 14884-900 JaboticabalDepartmento de Tecnologia FCAV UNESP, Via de Acesso Prof Paulo D.C. s/n, 14884-900 JaboticabalDepartmento de Biologia Aplicada À Agropecuária FCAV UNESP, Via de Acesso Prof Paulo D.C. s/n, 14884-900 JaboticabalDepartmento de Tecnologia FCAV UNESP, Via de Acesso Prof Paulo D.C. s/n, 14884-900 JaboticabalUniversidade Estadual Paulista (Unesp)Ferreira, Lucia P [UNESP]Lemos, Eliana G.M [UNESP]Lemos, Manoel Victor F [UNESP]2014-05-27T11:20:25Z2014-05-27T11:20:25Z2002-03-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article163-168application/pdfhttp://dx.doi.org/10.1016/S0378-1097(01)00473-6FEMS Microbiology Letters, v. 208, n. 2, p. 163-168, 2002.0378-1097http://hdl.handle.net/11449/6684910.1016/S0378-1097(01)00473-6WOS:0001751525000022-s2.0-00370229522-s2.0-0037022952.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFEMS Microbiology Letters1.7350,790info:eu-repo/semantics/openAccess2024-06-07T15:31:47Zoai:repositorio.unesp.br:11449/66849Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:27:02.313622Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Transposon Tn1721 distribution among strains of Xylella fastidiosa
title Transposon Tn1721 distribution among strains of Xylella fastidiosa
spellingShingle Transposon Tn1721 distribution among strains of Xylella fastidiosa
Ferreira, Lucia P [UNESP]
PCR fragment
Transposon Tn1721
Xylella fastidiosa
bacterial DNA
bacterial chromosome
bacterial genome
bacterial strain
bacterium isolation
citrus fruit
controlled study
DNA template
gene amplification
gene deletion
gene duplication
genetic variability
Gram negative bacterium
nonhuman
plum
polymerase chain reaction
priority journal
transposon
xylell fastidiosa
Base Sequence
Citrus
Coffee
DNA Transposable Elements
DNA, Bacterial
Gammaproteobacteria
Molecular Sequence Data
Plant Diseases
Prunus
Sequence Alignment
Species Specificity
Animalia
Bacteria (microorganisms)
Fungi
Prunus domestica
transposons
Vitis
title_short Transposon Tn1721 distribution among strains of Xylella fastidiosa
title_full Transposon Tn1721 distribution among strains of Xylella fastidiosa
title_fullStr Transposon Tn1721 distribution among strains of Xylella fastidiosa
title_full_unstemmed Transposon Tn1721 distribution among strains of Xylella fastidiosa
title_sort Transposon Tn1721 distribution among strains of Xylella fastidiosa
author Ferreira, Lucia P [UNESP]
author_facet Ferreira, Lucia P [UNESP]
Lemos, Eliana G.M [UNESP]
Lemos, Manoel Victor F [UNESP]
author_role author
author2 Lemos, Eliana G.M [UNESP]
Lemos, Manoel Victor F [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Ferreira, Lucia P [UNESP]
Lemos, Eliana G.M [UNESP]
Lemos, Manoel Victor F [UNESP]
dc.subject.por.fl_str_mv PCR fragment
Transposon Tn1721
Xylella fastidiosa
bacterial DNA
bacterial chromosome
bacterial genome
bacterial strain
bacterium isolation
citrus fruit
controlled study
DNA template
gene amplification
gene deletion
gene duplication
genetic variability
Gram negative bacterium
nonhuman
plum
polymerase chain reaction
priority journal
transposon
xylell fastidiosa
Base Sequence
Citrus
Coffee
DNA Transposable Elements
DNA, Bacterial
Gammaproteobacteria
Molecular Sequence Data
Plant Diseases
Prunus
Sequence Alignment
Species Specificity
Animalia
Bacteria (microorganisms)
Fungi
Prunus domestica
transposons
Vitis
topic PCR fragment
Transposon Tn1721
Xylella fastidiosa
bacterial DNA
bacterial chromosome
bacterial genome
bacterial strain
bacterium isolation
citrus fruit
controlled study
DNA template
gene amplification
gene deletion
gene duplication
genetic variability
Gram negative bacterium
nonhuman
plum
polymerase chain reaction
priority journal
transposon
xylell fastidiosa
Base Sequence
Citrus
Coffee
DNA Transposable Elements
DNA, Bacterial
Gammaproteobacteria
Molecular Sequence Data
Plant Diseases
Prunus
Sequence Alignment
Species Specificity
Animalia
Bacteria (microorganisms)
Fungi
Prunus domestica
transposons
Vitis
description Transposons are mobile genetic elements found within the genomes of various organisms including bacteria, fungi, plants and animals. Fragments of the transposon Tn1721 were found included in the genome of Xylella fastidiosa strain 9a5c. Regions from such fragments were PCR-amplified using specially designed primers (TNP1 and TNP2). In order to detect insertions of the Tn1721 element, both primers were used and one of them included a region of the transposon (TNP1) and the other one had the right repeat and part of the bacterial chromosome (TNP2). The PCR products obtained from strain 9a5c were used as a pattern for fragment size comparisons when DNA samples from other X. fastidiosa strains were used as template for the PCR assays. Differences were observed concerning the PCR products of such amplifications when some X. fastidiosa strains isolated from grapevine and plum were used. For the citrus-derived strains only the strains U187d and GP920b produced fragments with different sizes or weak band intensity. Such variations in the X. fastidiosa genome related to disrupted Tn1721 copies are probably due to the possibility of such a transposon element being still able to duplicate even after deletion events might have taken place and also because the bacterial strains in which the main differences were detected are derived from different host plants cultivated under different climate conditions from the one used as reference. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
publishDate 2002
dc.date.none.fl_str_mv 2002-03-05
2014-05-27T11:20:25Z
2014-05-27T11:20:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/S0378-1097(01)00473-6
FEMS Microbiology Letters, v. 208, n. 2, p. 163-168, 2002.
0378-1097
http://hdl.handle.net/11449/66849
10.1016/S0378-1097(01)00473-6
WOS:000175152500002
2-s2.0-0037022952
2-s2.0-0037022952.pdf
url http://dx.doi.org/10.1016/S0378-1097(01)00473-6
http://hdl.handle.net/11449/66849
identifier_str_mv FEMS Microbiology Letters, v. 208, n. 2, p. 163-168, 2002.
0378-1097
10.1016/S0378-1097(01)00473-6
WOS:000175152500002
2-s2.0-0037022952
2-s2.0-0037022952.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv FEMS Microbiology Letters
1.735
0,790
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 163-168
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
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institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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