Transposon Tn1721 distribution among strains of Xylella fastidiosa
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/S0378-1097(01)00473-6 http://hdl.handle.net/11449/66849 |
Resumo: | Transposons are mobile genetic elements found within the genomes of various organisms including bacteria, fungi, plants and animals. Fragments of the transposon Tn1721 were found included in the genome of Xylella fastidiosa strain 9a5c. Regions from such fragments were PCR-amplified using specially designed primers (TNP1 and TNP2). In order to detect insertions of the Tn1721 element, both primers were used and one of them included a region of the transposon (TNP1) and the other one had the right repeat and part of the bacterial chromosome (TNP2). The PCR products obtained from strain 9a5c were used as a pattern for fragment size comparisons when DNA samples from other X. fastidiosa strains were used as template for the PCR assays. Differences were observed concerning the PCR products of such amplifications when some X. fastidiosa strains isolated from grapevine and plum were used. For the citrus-derived strains only the strains U187d and GP920b produced fragments with different sizes or weak band intensity. Such variations in the X. fastidiosa genome related to disrupted Tn1721 copies are probably due to the possibility of such a transposon element being still able to duplicate even after deletion events might have taken place and also because the bacterial strains in which the main differences were detected are derived from different host plants cultivated under different climate conditions from the one used as reference. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. |
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Transposon Tn1721 distribution among strains of Xylella fastidiosaPCR fragmentTransposon Tn1721Xylella fastidiosabacterial DNAbacterial chromosomebacterial genomebacterial strainbacterium isolationcitrus fruitcontrolled studyDNA templategene amplificationgene deletiongene duplicationgenetic variabilityGram negative bacteriumnonhumanplumpolymerase chain reactionpriority journaltransposonxylell fastidiosaBase SequenceCitrusCoffeeDNA Transposable ElementsDNA, BacterialGammaproteobacteriaMolecular Sequence DataPlant DiseasesPrunusSequence AlignmentSpecies SpecificityAnimaliaBacteria (microorganisms)FungiPrunus domesticatransposonsVitisTransposons are mobile genetic elements found within the genomes of various organisms including bacteria, fungi, plants and animals. Fragments of the transposon Tn1721 were found included in the genome of Xylella fastidiosa strain 9a5c. Regions from such fragments were PCR-amplified using specially designed primers (TNP1 and TNP2). In order to detect insertions of the Tn1721 element, both primers were used and one of them included a region of the transposon (TNP1) and the other one had the right repeat and part of the bacterial chromosome (TNP2). The PCR products obtained from strain 9a5c were used as a pattern for fragment size comparisons when DNA samples from other X. fastidiosa strains were used as template for the PCR assays. Differences were observed concerning the PCR products of such amplifications when some X. fastidiosa strains isolated from grapevine and plum were used. For the citrus-derived strains only the strains U187d and GP920b produced fragments with different sizes or weak band intensity. Such variations in the X. fastidiosa genome related to disrupted Tn1721 copies are probably due to the possibility of such a transposon element being still able to duplicate even after deletion events might have taken place and also because the bacterial strains in which the main differences were detected are derived from different host plants cultivated under different climate conditions from the one used as reference. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.Departmento de Biologia Aplicada À Agropecuária FCAV UNESP, Via de Acesso Prof Paulo D.C. s/n, 14884-900 JaboticabalDepartmento de Tecnologia FCAV UNESP, Via de Acesso Prof Paulo D.C. s/n, 14884-900 JaboticabalDepartmento de Biologia Aplicada À Agropecuária FCAV UNESP, Via de Acesso Prof Paulo D.C. s/n, 14884-900 JaboticabalDepartmento de Tecnologia FCAV UNESP, Via de Acesso Prof Paulo D.C. s/n, 14884-900 JaboticabalUniversidade Estadual Paulista (Unesp)Ferreira, Lucia P [UNESP]Lemos, Eliana G.M [UNESP]Lemos, Manoel Victor F [UNESP]2014-05-27T11:20:25Z2014-05-27T11:20:25Z2002-03-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article163-168application/pdfhttp://dx.doi.org/10.1016/S0378-1097(01)00473-6FEMS Microbiology Letters, v. 208, n. 2, p. 163-168, 2002.0378-1097http://hdl.handle.net/11449/6684910.1016/S0378-1097(01)00473-6WOS:0001751525000022-s2.0-00370229522-s2.0-0037022952.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFEMS Microbiology Letters1.7350,790info:eu-repo/semantics/openAccess2024-06-07T15:31:47Zoai:repositorio.unesp.br:11449/66849Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:27:02.313622Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Transposon Tn1721 distribution among strains of Xylella fastidiosa |
title |
Transposon Tn1721 distribution among strains of Xylella fastidiosa |
spellingShingle |
Transposon Tn1721 distribution among strains of Xylella fastidiosa Ferreira, Lucia P [UNESP] PCR fragment Transposon Tn1721 Xylella fastidiosa bacterial DNA bacterial chromosome bacterial genome bacterial strain bacterium isolation citrus fruit controlled study DNA template gene amplification gene deletion gene duplication genetic variability Gram negative bacterium nonhuman plum polymerase chain reaction priority journal transposon xylell fastidiosa Base Sequence Citrus Coffee DNA Transposable Elements DNA, Bacterial Gammaproteobacteria Molecular Sequence Data Plant Diseases Prunus Sequence Alignment Species Specificity Animalia Bacteria (microorganisms) Fungi Prunus domestica transposons Vitis |
title_short |
Transposon Tn1721 distribution among strains of Xylella fastidiosa |
title_full |
Transposon Tn1721 distribution among strains of Xylella fastidiosa |
title_fullStr |
Transposon Tn1721 distribution among strains of Xylella fastidiosa |
title_full_unstemmed |
Transposon Tn1721 distribution among strains of Xylella fastidiosa |
title_sort |
Transposon Tn1721 distribution among strains of Xylella fastidiosa |
author |
Ferreira, Lucia P [UNESP] |
author_facet |
Ferreira, Lucia P [UNESP] Lemos, Eliana G.M [UNESP] Lemos, Manoel Victor F [UNESP] |
author_role |
author |
author2 |
Lemos, Eliana G.M [UNESP] Lemos, Manoel Victor F [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ferreira, Lucia P [UNESP] Lemos, Eliana G.M [UNESP] Lemos, Manoel Victor F [UNESP] |
dc.subject.por.fl_str_mv |
PCR fragment Transposon Tn1721 Xylella fastidiosa bacterial DNA bacterial chromosome bacterial genome bacterial strain bacterium isolation citrus fruit controlled study DNA template gene amplification gene deletion gene duplication genetic variability Gram negative bacterium nonhuman plum polymerase chain reaction priority journal transposon xylell fastidiosa Base Sequence Citrus Coffee DNA Transposable Elements DNA, Bacterial Gammaproteobacteria Molecular Sequence Data Plant Diseases Prunus Sequence Alignment Species Specificity Animalia Bacteria (microorganisms) Fungi Prunus domestica transposons Vitis |
topic |
PCR fragment Transposon Tn1721 Xylella fastidiosa bacterial DNA bacterial chromosome bacterial genome bacterial strain bacterium isolation citrus fruit controlled study DNA template gene amplification gene deletion gene duplication genetic variability Gram negative bacterium nonhuman plum polymerase chain reaction priority journal transposon xylell fastidiosa Base Sequence Citrus Coffee DNA Transposable Elements DNA, Bacterial Gammaproteobacteria Molecular Sequence Data Plant Diseases Prunus Sequence Alignment Species Specificity Animalia Bacteria (microorganisms) Fungi Prunus domestica transposons Vitis |
description |
Transposons are mobile genetic elements found within the genomes of various organisms including bacteria, fungi, plants and animals. Fragments of the transposon Tn1721 were found included in the genome of Xylella fastidiosa strain 9a5c. Regions from such fragments were PCR-amplified using specially designed primers (TNP1 and TNP2). In order to detect insertions of the Tn1721 element, both primers were used and one of them included a region of the transposon (TNP1) and the other one had the right repeat and part of the bacterial chromosome (TNP2). The PCR products obtained from strain 9a5c were used as a pattern for fragment size comparisons when DNA samples from other X. fastidiosa strains were used as template for the PCR assays. Differences were observed concerning the PCR products of such amplifications when some X. fastidiosa strains isolated from grapevine and plum were used. For the citrus-derived strains only the strains U187d and GP920b produced fragments with different sizes or weak band intensity. Such variations in the X. fastidiosa genome related to disrupted Tn1721 copies are probably due to the possibility of such a transposon element being still able to duplicate even after deletion events might have taken place and also because the bacterial strains in which the main differences were detected are derived from different host plants cultivated under different climate conditions from the one used as reference. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-03-05 2014-05-27T11:20:25Z 2014-05-27T11:20:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/S0378-1097(01)00473-6 FEMS Microbiology Letters, v. 208, n. 2, p. 163-168, 2002. 0378-1097 http://hdl.handle.net/11449/66849 10.1016/S0378-1097(01)00473-6 WOS:000175152500002 2-s2.0-0037022952 2-s2.0-0037022952.pdf |
url |
http://dx.doi.org/10.1016/S0378-1097(01)00473-6 http://hdl.handle.net/11449/66849 |
identifier_str_mv |
FEMS Microbiology Letters, v. 208, n. 2, p. 163-168, 2002. 0378-1097 10.1016/S0378-1097(01)00473-6 WOS:000175152500002 2-s2.0-0037022952 2-s2.0-0037022952.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
FEMS Microbiology Letters 1.735 0,790 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
163-168 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128814171029504 |