Plant Metabolomics Using NMR Spectroscopy

Detalhes bibliográficos
Autor(a) principal: Selegato, Denise Medeiros [UNESP]
Data de Publicação: 2019
Outros Autores: Pilon, Alan Cesar, Carnevale Neto, Fausto
Tipo de documento: Capítulo de livro
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/978-1-4939-9690-2_19
http://hdl.handle.net/11449/189590
Resumo: The major goal in plant metabolomics is to study complex extracts for the purposes of metabolic exploration and natural products discovery. To achieve this goal, plant metabolomics relies on accurate and selective acquisition of all possible chemical information, which includes maximization of the number of detected metabolites and their correct molecular assignment. Nuclear magnetic resonance (NMR) spectroscopy has been recognized as a powerful platform for obtaining the metabolite profiles of plant extracts. In this chapter, we provide a workflow for targeted and untargeted metabolite profiling of plant extracts using both 1D and 2D NMR methods. The protocol includes sample preparation, instrument operation, data processing, multivariate analysis, biomarker elucidation, and metabolite quantitation. It also addresses the annotation of plant metabolite peaks considering NMR’s capabilities to cover a broad range of metabolites and elucidate structures for unknown compounds.
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spelling Plant Metabolomics Using NMR SpectroscopyMetabolite annotationNatural productsNMR spectroscopyPlant metabolomicsQuantitative NMRThe major goal in plant metabolomics is to study complex extracts for the purposes of metabolic exploration and natural products discovery. To achieve this goal, plant metabolomics relies on accurate and selective acquisition of all possible chemical information, which includes maximization of the number of detected metabolites and their correct molecular assignment. Nuclear magnetic resonance (NMR) spectroscopy has been recognized as a powerful platform for obtaining the metabolite profiles of plant extracts. In this chapter, we provide a workflow for targeted and untargeted metabolite profiling of plant extracts using both 1D and 2D NMR methods. The protocol includes sample preparation, instrument operation, data processing, multivariate analysis, biomarker elucidation, and metabolite quantitation. It also addresses the annotation of plant metabolite peaks considering NMR’s capabilities to cover a broad range of metabolites and elucidate structures for unknown compounds.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)University of WashingtonNúcleo de Bioensaios Biossíntese e Ecofisiologia de Produtos Naturais (NuBBE) Departamento de Química Orgânica Instituto de Química Universidade Estadual Paulista (UNESP)Núcleo de Pesquisa em Produtos Naturais e Sintéticos (NPPNS) Departamento de Física e Química Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São PauloDepartment of Anesthesiology and Pain Medicine Northwest Metabolomics Research Center University of WashingtonNúcleo de Bioensaios Biossíntese e Ecofisiologia de Produtos Naturais (NuBBE) Departamento de Química Orgânica Instituto de Química Universidade Estadual Paulista (UNESP)FAPESP: 2014/ 05935-0FAPESP: 2016/13292-8FAPESP: 2017/06466-2Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)University of WashingtonSelegato, Denise Medeiros [UNESP]Pilon, Alan CesarCarnevale Neto, Fausto2019-10-06T16:45:35Z2019-10-06T16:45:35Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookPart345-362http://dx.doi.org/10.1007/978-1-4939-9690-2_19Methods in Molecular Biology, v. 2037, p. 345-362.1940-60291064-3745http://hdl.handle.net/11449/18959010.1007/978-1-4939-9690-2_192-s2.0-85071621577Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMethods in Molecular Biologyinfo:eu-repo/semantics/openAccess2021-10-23T01:58:02Zoai:repositorio.unesp.br:11449/189590Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:26:37.900960Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Plant Metabolomics Using NMR Spectroscopy
title Plant Metabolomics Using NMR Spectroscopy
spellingShingle Plant Metabolomics Using NMR Spectroscopy
Selegato, Denise Medeiros [UNESP]
Metabolite annotation
Natural products
NMR spectroscopy
Plant metabolomics
Quantitative NMR
title_short Plant Metabolomics Using NMR Spectroscopy
title_full Plant Metabolomics Using NMR Spectroscopy
title_fullStr Plant Metabolomics Using NMR Spectroscopy
title_full_unstemmed Plant Metabolomics Using NMR Spectroscopy
title_sort Plant Metabolomics Using NMR Spectroscopy
author Selegato, Denise Medeiros [UNESP]
author_facet Selegato, Denise Medeiros [UNESP]
Pilon, Alan Cesar
Carnevale Neto, Fausto
author_role author
author2 Pilon, Alan Cesar
Carnevale Neto, Fausto
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
University of Washington
dc.contributor.author.fl_str_mv Selegato, Denise Medeiros [UNESP]
Pilon, Alan Cesar
Carnevale Neto, Fausto
dc.subject.por.fl_str_mv Metabolite annotation
Natural products
NMR spectroscopy
Plant metabolomics
Quantitative NMR
topic Metabolite annotation
Natural products
NMR spectroscopy
Plant metabolomics
Quantitative NMR
description The major goal in plant metabolomics is to study complex extracts for the purposes of metabolic exploration and natural products discovery. To achieve this goal, plant metabolomics relies on accurate and selective acquisition of all possible chemical information, which includes maximization of the number of detected metabolites and their correct molecular assignment. Nuclear magnetic resonance (NMR) spectroscopy has been recognized as a powerful platform for obtaining the metabolite profiles of plant extracts. In this chapter, we provide a workflow for targeted and untargeted metabolite profiling of plant extracts using both 1D and 2D NMR methods. The protocol includes sample preparation, instrument operation, data processing, multivariate analysis, biomarker elucidation, and metabolite quantitation. It also addresses the annotation of plant metabolite peaks considering NMR’s capabilities to cover a broad range of metabolites and elucidate structures for unknown compounds.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:45:35Z
2019-10-06T16:45:35Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bookPart
format bookPart
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/978-1-4939-9690-2_19
Methods in Molecular Biology, v. 2037, p. 345-362.
1940-6029
1064-3745
http://hdl.handle.net/11449/189590
10.1007/978-1-4939-9690-2_19
2-s2.0-85071621577
url http://dx.doi.org/10.1007/978-1-4939-9690-2_19
http://hdl.handle.net/11449/189590
identifier_str_mv Methods in Molecular Biology, v. 2037, p. 345-362.
1940-6029
1064-3745
10.1007/978-1-4939-9690-2_19
2-s2.0-85071621577
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Methods in Molecular Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 345-362
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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