Non-destructive genetic sampling in fish. An improved method for DNA extraction from fish fins and scales
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1034/j.1601-5223.2003.01503.x http://hdl.handle.net/11449/18324 |
Resumo: | DNA-based studies have been one of the major interests in conservation biology of endangered species and in population genetics. As species and population genetic assessment requires a source of biological material, the sampling strategy can be overcome by non-destructive procedures for DNA isolation. An improved method for obtaining DNA from fish fins and scales with the use of an extraction buffer containing urea and further DNA purification with phenol-chloroform is described. The methodology combines the benefits of a non-destructive DNA sampling and its high efficiency. In addition, comparisons with other methodologies for isolating DNA from fish demonstrated that the present procedure also becomes a very attractive alternative to obtain large amounts of high-quality DNA for use in different molecular analyses. The DNA samples, isolated from different fish species, have been successfully used on random amplified polymorphic DNA (RAPD) experiments, as well as on amplification of specific ribosomal and mitochondrial DNA sequences. The present DNA extraction procedure represents an alternative for population approaches and genetic studies on rare or endangered taxa. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Non-destructive genetic sampling in fish. An improved method for DNA extraction from fish fins and scalesDNA-based studies have been one of the major interests in conservation biology of endangered species and in population genetics. As species and population genetic assessment requires a source of biological material, the sampling strategy can be overcome by non-destructive procedures for DNA isolation. An improved method for obtaining DNA from fish fins and scales with the use of an extraction buffer containing urea and further DNA purification with phenol-chloroform is described. The methodology combines the benefits of a non-destructive DNA sampling and its high efficiency. In addition, comparisons with other methodologies for isolating DNA from fish demonstrated that the present procedure also becomes a very attractive alternative to obtain large amounts of high-quality DNA for use in different molecular analyses. The DNA samples, isolated from different fish species, have been successfully used on random amplified polymorphic DNA (RAPD) experiments, as well as on amplification of specific ribosomal and mitochondrial DNA sequences. The present DNA extraction procedure represents an alternative for population approaches and genetic studies on rare or endangered taxa.Univ Estadual Paulista, Inst Biociencias, Dept Morfol, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Botucatu, SP, BrazilBlackwell MunksgaardUniversidade Estadual Paulista (Unesp)Wasko, A. P.Martins, C.Oliveira, C.Foresti, F.2014-05-20T13:51:18Z2014-05-20T13:51:18Z2003-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article161-165application/pdfhttp://dx.doi.org/10.1034/j.1601-5223.2003.01503.xHereditas. Copenhagen: Blackwell Munksgaard, v. 138, n. 3, p. 161-165, 2003.0018-0661http://hdl.handle.net/11449/1832410.1034/j.1601-5223.2003.01503.xWOS:000187074000001WOS000187074000001.pdf1926863917378909885880069942535208047939448463670000-0003-4600-03670000-0003-3534-974XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHereditas0,278info:eu-repo/semantics/openAccess2024-01-10T06:30:15Zoai:repositorio.unesp.br:11449/18324Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:39:19.758785Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Non-destructive genetic sampling in fish. An improved method for DNA extraction from fish fins and scales |
title |
Non-destructive genetic sampling in fish. An improved method for DNA extraction from fish fins and scales |
spellingShingle |
Non-destructive genetic sampling in fish. An improved method for DNA extraction from fish fins and scales Wasko, A. P. |
title_short |
Non-destructive genetic sampling in fish. An improved method for DNA extraction from fish fins and scales |
title_full |
Non-destructive genetic sampling in fish. An improved method for DNA extraction from fish fins and scales |
title_fullStr |
Non-destructive genetic sampling in fish. An improved method for DNA extraction from fish fins and scales |
title_full_unstemmed |
Non-destructive genetic sampling in fish. An improved method for DNA extraction from fish fins and scales |
title_sort |
Non-destructive genetic sampling in fish. An improved method for DNA extraction from fish fins and scales |
author |
Wasko, A. P. |
author_facet |
Wasko, A. P. Martins, C. Oliveira, C. Foresti, F. |
author_role |
author |
author2 |
Martins, C. Oliveira, C. Foresti, F. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Wasko, A. P. Martins, C. Oliveira, C. Foresti, F. |
description |
DNA-based studies have been one of the major interests in conservation biology of endangered species and in population genetics. As species and population genetic assessment requires a source of biological material, the sampling strategy can be overcome by non-destructive procedures for DNA isolation. An improved method for obtaining DNA from fish fins and scales with the use of an extraction buffer containing urea and further DNA purification with phenol-chloroform is described. The methodology combines the benefits of a non-destructive DNA sampling and its high efficiency. In addition, comparisons with other methodologies for isolating DNA from fish demonstrated that the present procedure also becomes a very attractive alternative to obtain large amounts of high-quality DNA for use in different molecular analyses. The DNA samples, isolated from different fish species, have been successfully used on random amplified polymorphic DNA (RAPD) experiments, as well as on amplification of specific ribosomal and mitochondrial DNA sequences. The present DNA extraction procedure represents an alternative for population approaches and genetic studies on rare or endangered taxa. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-01-01 2014-05-20T13:51:18Z 2014-05-20T13:51:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1034/j.1601-5223.2003.01503.x Hereditas. Copenhagen: Blackwell Munksgaard, v. 138, n. 3, p. 161-165, 2003. 0018-0661 http://hdl.handle.net/11449/18324 10.1034/j.1601-5223.2003.01503.x WOS:000187074000001 WOS000187074000001.pdf 1926863917378909 8858800699425352 0804793944846367 0000-0003-4600-0367 0000-0003-3534-974X |
url |
http://dx.doi.org/10.1034/j.1601-5223.2003.01503.x http://hdl.handle.net/11449/18324 |
identifier_str_mv |
Hereditas. Copenhagen: Blackwell Munksgaard, v. 138, n. 3, p. 161-165, 2003. 0018-0661 10.1034/j.1601-5223.2003.01503.x WOS:000187074000001 WOS000187074000001.pdf 1926863917378909 8858800699425352 0804793944846367 0000-0003-4600-0367 0000-0003-3534-974X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Hereditas 0,278 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
161-165 application/pdf |
dc.publisher.none.fl_str_mv |
Blackwell Munksgaard |
publisher.none.fl_str_mv |
Blackwell Munksgaard |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129447431241728 |