Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1984-29612017046 http://hdl.handle.net/11449/175299 |
Resumo: | Equine piroplasmosisis, a tick-borne disease caused by the intra-erythrocytic protozoans Babesia caballi and Theileria equi, has economic importance due to the international trade and the increased movement of horses all over the world. The goal of this study was to evaluate the occurrence of phylogenetic diversity of T. equi and B. caballi genotypes among infected equids from São Luís Island, state of Maranhão, northeastern Brazil. Between December of 2011 and June of 2012, EDTA-blood and serum samples were collected from 139 equids (90 donkeys, 39 horses and 10 mules). From 139 serum samples submitted to ELISA assay, IgG antibodies to T. equi and B. caballi were detected in 19.4% (27/139) and 25.2% (35/139), respectively. Among sampled animals, 21.6% (30/139) and 55.4% (77/139) were positive for cPCR assays for T. equi and B. caballi, based on ema-1 and rap-1 genes, respectively. Overall, the T. equi sequences (n=7) submitted to Maximum Likelihood analysis (based on a 18S rRNA fragment of 1700 bp after alignment) grouped into three main groups, which were subdivided in eight clusters. The present work showed that different genotypes of T. equi and B. caballi circulate among equids in Brazil. |
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Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern BrazilDiversidade genética de espécies de piroplasmídeos em equídeos na ilha de São Luís, Nordeste do Brasil18S rRNABabesia caballiPhylogenyTheileria equiEquine piroplasmosisis, a tick-borne disease caused by the intra-erythrocytic protozoans Babesia caballi and Theileria equi, has economic importance due to the international trade and the increased movement of horses all over the world. The goal of this study was to evaluate the occurrence of phylogenetic diversity of T. equi and B. caballi genotypes among infected equids from São Luís Island, state of Maranhão, northeastern Brazil. Between December of 2011 and June of 2012, EDTA-blood and serum samples were collected from 139 equids (90 donkeys, 39 horses and 10 mules). From 139 serum samples submitted to ELISA assay, IgG antibodies to T. equi and B. caballi were detected in 19.4% (27/139) and 25.2% (35/139), respectively. Among sampled animals, 21.6% (30/139) and 55.4% (77/139) were positive for cPCR assays for T. equi and B. caballi, based on ema-1 and rap-1 genes, respectively. Overall, the T. equi sequences (n=7) submitted to Maximum Likelihood analysis (based on a 18S rRNA fragment of 1700 bp after alignment) grouped into three main groups, which were subdivided in eight clusters. The present work showed that different genotypes of T. equi and B. caballi circulate among equids in Brazil.Universidade Estadual do Maranhão-UEMALaboratório de Imunoparasitologia Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias-FCAV Universidade Estadual Paulista-UNESPLaboratório de Imunoparasitologia Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias-FCAV Universidade Estadual Paulista-UNESPUniversidade Estadual de Maringá (UEM)Universidade Estadual Paulista (Unesp)Braga, Maria do Socorro Costa de OliveiraCosta, Francisca NeideGomes, Débora Regina MaiaXavier, Daniele RosaAndré, Marcos Rogério [UNESP]Gonçalves, Luiz Ricardo [UNESP]Freschi, Carla Roberta [UNESP]Machado, Rosangela Zacarias [UNESP]2018-12-11T17:15:12Z2018-12-11T17:15:12Z2017-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article331-339application/pdfhttp://dx.doi.org/10.1590/S1984-29612017046Revista Brasileira de Parasitologia Veterinaria, v. 26, n. 3, p. 331-339, 2017.0103-846Xhttp://hdl.handle.net/11449/17529910.1590/S1984-29612017046S1984-296120170003003312-s2.0-85030669895S1984-29612017000300331.pdf9139899895580513Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2024-06-07T13:02:38Zoai:repositorio.unesp.br:11449/175299Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:15:17.539838Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil Diversidade genética de espécies de piroplasmídeos em equídeos na ilha de São Luís, Nordeste do Brasil |
title |
Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil |
spellingShingle |
Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil Braga, Maria do Socorro Costa de Oliveira 18S rRNA Babesia caballi Phylogeny Theileria equi |
title_short |
Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil |
title_full |
Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil |
title_fullStr |
Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil |
title_full_unstemmed |
Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil |
title_sort |
Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil |
author |
Braga, Maria do Socorro Costa de Oliveira |
author_facet |
Braga, Maria do Socorro Costa de Oliveira Costa, Francisca Neide Gomes, Débora Regina Maia Xavier, Daniele Rosa André, Marcos Rogério [UNESP] Gonçalves, Luiz Ricardo [UNESP] Freschi, Carla Roberta [UNESP] Machado, Rosangela Zacarias [UNESP] |
author_role |
author |
author2 |
Costa, Francisca Neide Gomes, Débora Regina Maia Xavier, Daniele Rosa André, Marcos Rogério [UNESP] Gonçalves, Luiz Ricardo [UNESP] Freschi, Carla Roberta [UNESP] Machado, Rosangela Zacarias [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Maringá (UEM) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Braga, Maria do Socorro Costa de Oliveira Costa, Francisca Neide Gomes, Débora Regina Maia Xavier, Daniele Rosa André, Marcos Rogério [UNESP] Gonçalves, Luiz Ricardo [UNESP] Freschi, Carla Roberta [UNESP] Machado, Rosangela Zacarias [UNESP] |
dc.subject.por.fl_str_mv |
18S rRNA Babesia caballi Phylogeny Theileria equi |
topic |
18S rRNA Babesia caballi Phylogeny Theileria equi |
description |
Equine piroplasmosisis, a tick-borne disease caused by the intra-erythrocytic protozoans Babesia caballi and Theileria equi, has economic importance due to the international trade and the increased movement of horses all over the world. The goal of this study was to evaluate the occurrence of phylogenetic diversity of T. equi and B. caballi genotypes among infected equids from São Luís Island, state of Maranhão, northeastern Brazil. Between December of 2011 and June of 2012, EDTA-blood and serum samples were collected from 139 equids (90 donkeys, 39 horses and 10 mules). From 139 serum samples submitted to ELISA assay, IgG antibodies to T. equi and B. caballi were detected in 19.4% (27/139) and 25.2% (35/139), respectively. Among sampled animals, 21.6% (30/139) and 55.4% (77/139) were positive for cPCR assays for T. equi and B. caballi, based on ema-1 and rap-1 genes, respectively. Overall, the T. equi sequences (n=7) submitted to Maximum Likelihood analysis (based on a 18S rRNA fragment of 1700 bp after alignment) grouped into three main groups, which were subdivided in eight clusters. The present work showed that different genotypes of T. equi and B. caballi circulate among equids in Brazil. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-01 2018-12-11T17:15:12Z 2018-12-11T17:15:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1984-29612017046 Revista Brasileira de Parasitologia Veterinaria, v. 26, n. 3, p. 331-339, 2017. 0103-846X http://hdl.handle.net/11449/175299 10.1590/S1984-29612017046 S1984-29612017000300331 2-s2.0-85030669895 S1984-29612017000300331.pdf 9139899895580513 |
url |
http://dx.doi.org/10.1590/S1984-29612017046 http://hdl.handle.net/11449/175299 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 26, n. 3, p. 331-339, 2017. 0103-846X 10.1590/S1984-29612017046 S1984-29612017000300331 2-s2.0-85030669895 S1984-29612017000300331.pdf 9139899895580513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
331-339 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129179657437184 |