Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil

Detalhes bibliográficos
Autor(a) principal: Braga, Maria do Socorro Costa de Oliveira
Data de Publicação: 2017
Outros Autores: Costa, Francisca Neide, Gomes, Débora Regina Maia, Xavier, Daniele Rosa, André, Marcos Rogério [UNESP], Gonçalves, Luiz Ricardo [UNESP], Freschi, Carla Roberta [UNESP], Machado, Rosangela Zacarias [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1984-29612017046
http://hdl.handle.net/11449/175299
Resumo: Equine piroplasmosisis, a tick-borne disease caused by the intra-erythrocytic protozoans Babesia caballi and Theileria equi, has economic importance due to the international trade and the increased movement of horses all over the world. The goal of this study was to evaluate the occurrence of phylogenetic diversity of T. equi and B. caballi genotypes among infected equids from São Luís Island, state of Maranhão, northeastern Brazil. Between December of 2011 and June of 2012, EDTA-blood and serum samples were collected from 139 equids (90 donkeys, 39 horses and 10 mules). From 139 serum samples submitted to ELISA assay, IgG antibodies to T. equi and B. caballi were detected in 19.4% (27/139) and 25.2% (35/139), respectively. Among sampled animals, 21.6% (30/139) and 55.4% (77/139) were positive for cPCR assays for T. equi and B. caballi, based on ema-1 and rap-1 genes, respectively. Overall, the T. equi sequences (n=7) submitted to Maximum Likelihood analysis (based on a 18S rRNA fragment of 1700 bp after alignment) grouped into three main groups, which were subdivided in eight clusters. The present work showed that different genotypes of T. equi and B. caballi circulate among equids in Brazil.
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spelling Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern BrazilDiversidade genética de espécies de piroplasmídeos em equídeos na ilha de São Luís, Nordeste do Brasil18S rRNABabesia caballiPhylogenyTheileria equiEquine piroplasmosisis, a tick-borne disease caused by the intra-erythrocytic protozoans Babesia caballi and Theileria equi, has economic importance due to the international trade and the increased movement of horses all over the world. The goal of this study was to evaluate the occurrence of phylogenetic diversity of T. equi and B. caballi genotypes among infected equids from São Luís Island, state of Maranhão, northeastern Brazil. Between December of 2011 and June of 2012, EDTA-blood and serum samples were collected from 139 equids (90 donkeys, 39 horses and 10 mules). From 139 serum samples submitted to ELISA assay, IgG antibodies to T. equi and B. caballi were detected in 19.4% (27/139) and 25.2% (35/139), respectively. Among sampled animals, 21.6% (30/139) and 55.4% (77/139) were positive for cPCR assays for T. equi and B. caballi, based on ema-1 and rap-1 genes, respectively. Overall, the T. equi sequences (n=7) submitted to Maximum Likelihood analysis (based on a 18S rRNA fragment of 1700 bp after alignment) grouped into three main groups, which were subdivided in eight clusters. The present work showed that different genotypes of T. equi and B. caballi circulate among equids in Brazil.Universidade Estadual do Maranhão-UEMALaboratório de Imunoparasitologia Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias-FCAV Universidade Estadual Paulista-UNESPLaboratório de Imunoparasitologia Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias-FCAV Universidade Estadual Paulista-UNESPUniversidade Estadual de Maringá (UEM)Universidade Estadual Paulista (Unesp)Braga, Maria do Socorro Costa de OliveiraCosta, Francisca NeideGomes, Débora Regina MaiaXavier, Daniele RosaAndré, Marcos Rogério [UNESP]Gonçalves, Luiz Ricardo [UNESP]Freschi, Carla Roberta [UNESP]Machado, Rosangela Zacarias [UNESP]2018-12-11T17:15:12Z2018-12-11T17:15:12Z2017-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article331-339application/pdfhttp://dx.doi.org/10.1590/S1984-29612017046Revista Brasileira de Parasitologia Veterinaria, v. 26, n. 3, p. 331-339, 2017.0103-846Xhttp://hdl.handle.net/11449/17529910.1590/S1984-29612017046S1984-296120170003003312-s2.0-85030669895S1984-29612017000300331.pdf9139899895580513Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2024-06-07T13:02:38Zoai:repositorio.unesp.br:11449/175299Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:15:17.539838Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil
Diversidade genética de espécies de piroplasmídeos em equídeos na ilha de São Luís, Nordeste do Brasil
title Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil
spellingShingle Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil
Braga, Maria do Socorro Costa de Oliveira
18S rRNA
Babesia caballi
Phylogeny
Theileria equi
title_short Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil
title_full Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil
title_fullStr Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil
title_full_unstemmed Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil
title_sort Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil
author Braga, Maria do Socorro Costa de Oliveira
author_facet Braga, Maria do Socorro Costa de Oliveira
Costa, Francisca Neide
Gomes, Débora Regina Maia
Xavier, Daniele Rosa
André, Marcos Rogério [UNESP]
Gonçalves, Luiz Ricardo [UNESP]
Freschi, Carla Roberta [UNESP]
Machado, Rosangela Zacarias [UNESP]
author_role author
author2 Costa, Francisca Neide
Gomes, Débora Regina Maia
Xavier, Daniele Rosa
André, Marcos Rogério [UNESP]
Gonçalves, Luiz Ricardo [UNESP]
Freschi, Carla Roberta [UNESP]
Machado, Rosangela Zacarias [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Maringá (UEM)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Braga, Maria do Socorro Costa de Oliveira
Costa, Francisca Neide
Gomes, Débora Regina Maia
Xavier, Daniele Rosa
André, Marcos Rogério [UNESP]
Gonçalves, Luiz Ricardo [UNESP]
Freschi, Carla Roberta [UNESP]
Machado, Rosangela Zacarias [UNESP]
dc.subject.por.fl_str_mv 18S rRNA
Babesia caballi
Phylogeny
Theileria equi
topic 18S rRNA
Babesia caballi
Phylogeny
Theileria equi
description Equine piroplasmosisis, a tick-borne disease caused by the intra-erythrocytic protozoans Babesia caballi and Theileria equi, has economic importance due to the international trade and the increased movement of horses all over the world. The goal of this study was to evaluate the occurrence of phylogenetic diversity of T. equi and B. caballi genotypes among infected equids from São Luís Island, state of Maranhão, northeastern Brazil. Between December of 2011 and June of 2012, EDTA-blood and serum samples were collected from 139 equids (90 donkeys, 39 horses and 10 mules). From 139 serum samples submitted to ELISA assay, IgG antibodies to T. equi and B. caballi were detected in 19.4% (27/139) and 25.2% (35/139), respectively. Among sampled animals, 21.6% (30/139) and 55.4% (77/139) were positive for cPCR assays for T. equi and B. caballi, based on ema-1 and rap-1 genes, respectively. Overall, the T. equi sequences (n=7) submitted to Maximum Likelihood analysis (based on a 18S rRNA fragment of 1700 bp after alignment) grouped into three main groups, which were subdivided in eight clusters. The present work showed that different genotypes of T. equi and B. caballi circulate among equids in Brazil.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-01
2018-12-11T17:15:12Z
2018-12-11T17:15:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1984-29612017046
Revista Brasileira de Parasitologia Veterinaria, v. 26, n. 3, p. 331-339, 2017.
0103-846X
http://hdl.handle.net/11449/175299
10.1590/S1984-29612017046
S1984-29612017000300331
2-s2.0-85030669895
S1984-29612017000300331.pdf
9139899895580513
url http://dx.doi.org/10.1590/S1984-29612017046
http://hdl.handle.net/11449/175299
identifier_str_mv Revista Brasileira de Parasitologia Veterinaria, v. 26, n. 3, p. 331-339, 2017.
0103-846X
10.1590/S1984-29612017046
S1984-29612017000300331
2-s2.0-85030669895
S1984-29612017000300331.pdf
9139899895580513
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Parasitologia Veterinaria
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 331-339
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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