Modulation of gene expression and inflammation but not DNA damage after sevoflurane anesthesia

Detalhes bibliográficos
Autor(a) principal: Silva, Mariane A. P. [UNESP]
Data de Publicação: 2023
Outros Autores: Braz, Leandro G. [UNESP], Braz, José Reinaldo C. [UNESP], Braz, Mariana G. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/em.22539
http://hdl.handle.net/11449/247191
Resumo: This study assessed, for the first time, the expression of the genes hOGG1, TP53, and IL-6 in leukocytes by real-time quantitative polymerase chain reaction in surgical patients before (baseline), during (2 h of anesthesia) and 1 day after sevoflurane anesthesia. Additionally, DNA damage was detected by the comet assay, serum interleukin (IL)-6 was detected by flow cytometry, and differential leukocyte counting was also performed. TP53 and hOGG1 expression was downregulated on the day after anesthesia compared to before anesthesia. However, IL-6 expression did not change, and no DNA damage induction was observed during or after anesthesia. At the systemic level, mild neutrophilia and an increase in IL-6 levels occurred after anesthesia. Our findings suggest that sevoflurane anesthesia downregulates gene expression (hOGG1 and TP53) and contributes to an inflammatory status (increased systemic IL-6 and mild neutrophilia) but is not associated with DNA damage in patients without comorbidities who undergo minor elective surgery.
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spelling Modulation of gene expression and inflammation but not DNA damage after sevoflurane anesthesiacomet assaygene expressioninflammationinhalational anestheticminor surgical proceduresThis study assessed, for the first time, the expression of the genes hOGG1, TP53, and IL-6 in leukocytes by real-time quantitative polymerase chain reaction in surgical patients before (baseline), during (2 h of anesthesia) and 1 day after sevoflurane anesthesia. Additionally, DNA damage was detected by the comet assay, serum interleukin (IL)-6 was detected by flow cytometry, and differential leukocyte counting was also performed. TP53 and hOGG1 expression was downregulated on the day after anesthesia compared to before anesthesia. However, IL-6 expression did not change, and no DNA damage induction was observed during or after anesthesia. At the systemic level, mild neutrophilia and an increase in IL-6 levels occurred after anesthesia. Our findings suggest that sevoflurane anesthesia downregulates gene expression (hOGG1 and TP53) and contributes to an inflammatory status (increased systemic IL-6 and mild neutrophilia) but is not associated with DNA damage in patients without comorbidities who undergo minor elective surgery.GENOTOX Laboratory Botucatu Medical School São Paulo State University – UNESP, São PauloGENOTOX Laboratory Botucatu Medical School São Paulo State University – UNESP, São PauloUniversidade Estadual Paulista (UNESP)Silva, Mariane A. P. [UNESP]Braz, Leandro G. [UNESP]Braz, José Reinaldo C. [UNESP]Braz, Mariana G. [UNESP]2023-07-29T13:08:50Z2023-07-29T13:08:50Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/em.22539Environmental and Molecular Mutagenesis.1098-22800893-6692http://hdl.handle.net/11449/24719110.1002/em.225392-s2.0-85152780350Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental and Molecular Mutagenesisinfo:eu-repo/semantics/openAccess2023-07-29T13:08:50Zoai:repositorio.unesp.br:11449/247191Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:10:06.789060Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Modulation of gene expression and inflammation but not DNA damage after sevoflurane anesthesia
title Modulation of gene expression and inflammation but not DNA damage after sevoflurane anesthesia
spellingShingle Modulation of gene expression and inflammation but not DNA damage after sevoflurane anesthesia
Silva, Mariane A. P. [UNESP]
comet assay
gene expression
inflammation
inhalational anesthetic
minor surgical procedures
title_short Modulation of gene expression and inflammation but not DNA damage after sevoflurane anesthesia
title_full Modulation of gene expression and inflammation but not DNA damage after sevoflurane anesthesia
title_fullStr Modulation of gene expression and inflammation but not DNA damage after sevoflurane anesthesia
title_full_unstemmed Modulation of gene expression and inflammation but not DNA damage after sevoflurane anesthesia
title_sort Modulation of gene expression and inflammation but not DNA damage after sevoflurane anesthesia
author Silva, Mariane A. P. [UNESP]
author_facet Silva, Mariane A. P. [UNESP]
Braz, Leandro G. [UNESP]
Braz, José Reinaldo C. [UNESP]
Braz, Mariana G. [UNESP]
author_role author
author2 Braz, Leandro G. [UNESP]
Braz, José Reinaldo C. [UNESP]
Braz, Mariana G. [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Silva, Mariane A. P. [UNESP]
Braz, Leandro G. [UNESP]
Braz, José Reinaldo C. [UNESP]
Braz, Mariana G. [UNESP]
dc.subject.por.fl_str_mv comet assay
gene expression
inflammation
inhalational anesthetic
minor surgical procedures
topic comet assay
gene expression
inflammation
inhalational anesthetic
minor surgical procedures
description This study assessed, for the first time, the expression of the genes hOGG1, TP53, and IL-6 in leukocytes by real-time quantitative polymerase chain reaction in surgical patients before (baseline), during (2 h of anesthesia) and 1 day after sevoflurane anesthesia. Additionally, DNA damage was detected by the comet assay, serum interleukin (IL)-6 was detected by flow cytometry, and differential leukocyte counting was also performed. TP53 and hOGG1 expression was downregulated on the day after anesthesia compared to before anesthesia. However, IL-6 expression did not change, and no DNA damage induction was observed during or after anesthesia. At the systemic level, mild neutrophilia and an increase in IL-6 levels occurred after anesthesia. Our findings suggest that sevoflurane anesthesia downregulates gene expression (hOGG1 and TP53) and contributes to an inflammatory status (increased systemic IL-6 and mild neutrophilia) but is not associated with DNA damage in patients without comorbidities who undergo minor elective surgery.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:08:50Z
2023-07-29T13:08:50Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/em.22539
Environmental and Molecular Mutagenesis.
1098-2280
0893-6692
http://hdl.handle.net/11449/247191
10.1002/em.22539
2-s2.0-85152780350
url http://dx.doi.org/10.1002/em.22539
http://hdl.handle.net/11449/247191
identifier_str_mv Environmental and Molecular Mutagenesis.
1098-2280
0893-6692
10.1002/em.22539
2-s2.0-85152780350
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Environmental and Molecular Mutagenesis
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128473394315264