Characterization and polymorphism screening of IGF-I and prolactin genes in Nelore heifers

Detalhes bibliográficos
Autor(a) principal: Massolini Laureano, Monyka Marianna
Data de Publicação: 2009
Outros Autores: Otaviano, Antonio Roberto, Ferreira Lima, Andre Luis, Costa, Raphael Bermal, Dias Salman, Ana Karina, Desiderio Sena, Janete Apparecida [UNESP], Tonhati, Humberto [UNESP], Albuquerque, Lucia Galvão de [UNESP]
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.aspajournal.it/index.php/ijas/article/view/35
http://hdl.handle.net/11449/1063
Resumo: Insulin growth factor I (IGF-I) and prolactin (PRL) are peptide hormones that exert complementary effects on reproductive traits by acting on folliculogenesis. In view of the lack of information about the IGF-I and PRL genes in Bos indicus, the objective of this study was to partially characterize the promoter regions of these genes and to screen animals of different ages at first pregnancy for the presence of polymorphisms in these regions. In addition, we determined whether polymorphisms influence the regulation of the two hormone genes, evaluating their association with sexual precocity.The animals were divided into three groups according to age at first pregnancy: 1) 100 heifers considered to be sexually precocious that became pregnant at 15-16 months of age, 2) 100 heifers that became pregnant during the normal breeding season at 24 months of age, and 3) 100 heifers that did not become pregnant until 24 months of age. For the IGF-I gene, PCR-RFLP-SnaBI analysis showed the presence of genotypes AB and BB at frequencies of 0.02 and 0.98, respectively. Sequencing of the IGF-I gene fragment revealed a single nitrogen base change from cytosine to thymine, corresponding to the restriction site of SnaBI. The polymorphisms identified in the 5'-flanking region of the IGF-I gene may serve as a basis for future studies of molecular markers in cattle. For the PRL gene, PCR-RFLP-HaeIII analysis showed the presence of only one migration pattern, a finding characterizing the region studied as monomorphic. The study of other regions in the IGF-I and PRL genes might provide molecular data that can be used in the future for the selection of sexually precocious animals.
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spelling Characterization and polymorphism screening of IGF-I and prolactin genes in Nelore heifersBeef cattleMolecular markerRFLPSexual precocityInsulin growth factor I (IGF-I) and prolactin (PRL) are peptide hormones that exert complementary effects on reproductive traits by acting on folliculogenesis. In view of the lack of information about the IGF-I and PRL genes in Bos indicus, the objective of this study was to partially characterize the promoter regions of these genes and to screen animals of different ages at first pregnancy for the presence of polymorphisms in these regions. In addition, we determined whether polymorphisms influence the regulation of the two hormone genes, evaluating their association with sexual precocity.The animals were divided into three groups according to age at first pregnancy: 1) 100 heifers considered to be sexually precocious that became pregnant at 15-16 months of age, 2) 100 heifers that became pregnant during the normal breeding season at 24 months of age, and 3) 100 heifers that did not become pregnant until 24 months of age. For the IGF-I gene, PCR-RFLP-SnaBI analysis showed the presence of genotypes AB and BB at frequencies of 0.02 and 0.98, respectively. Sequencing of the IGF-I gene fragment revealed a single nitrogen base change from cytosine to thymine, corresponding to the restriction site of SnaBI. The polymorphisms identified in the 5'-flanking region of the IGF-I gene may serve as a basis for future studies of molecular markers in cattle. For the PRL gene, PCR-RFLP-HaeIII analysis showed the presence of only one migration pattern, a finding characterizing the region studied as monomorphic. The study of other regions in the IGF-I and PRL genes might provide molecular data that can be used in the future for the selection of sexually precocious animals.Univ Estadual Paulista, Dept Zootecnia, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP, BrazilCtr Pesquisa Agroflorestais Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Rondonia, Porto Velho, BrazilUniv Estadual Paulista, Dept Biol Aplicada Agropecuaria, BR-14884900 Jaboticabal, SP, BrazilCtr Nacl Desenvolvimento Cient & Tecnol, Brasilia, DF, BrazilUniv Estadual Paulista, Dept Zootecnia, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP, BrazilUniv Estadual Paulista, Dept Biol Aplicada Agropecuaria, BR-14884900 Jaboticabal, SP, BrazilAssoc Sci Produzione Animale-aspaUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Massolini Laureano, Monyka MariannaOtaviano, Antonio RobertoFerreira Lima, Andre LuisCosta, Raphael BermalDias Salman, Ana KarinaDesiderio Sena, Janete Apparecida [UNESP]Tonhati, Humberto [UNESP]Albuquerque, Lucia Galvão de [UNESP]2014-05-20T13:13:12Z2014-05-20T13:13:12Z2009-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject277-283application/pdfhttp://www.aspajournal.it/index.php/ijas/article/view/35Italian Journal of Animal Science. Reggio Emilia: Assoc Sci Produzione Animale-aspa, v. 8, n. 2, p. 277-283, 2009.1594-4077http://hdl.handle.net/11449/1063WOS:000268338200014WOS000268338200014.pdf74452549608581595866981114947883Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengItalian Journal of Animal Science0.9900,434info:eu-repo/semantics/openAccess2024-06-07T18:46:20Zoai:repositorio.unesp.br:11449/1063Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:44:42.124596Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Characterization and polymorphism screening of IGF-I and prolactin genes in Nelore heifers
title Characterization and polymorphism screening of IGF-I and prolactin genes in Nelore heifers
spellingShingle Characterization and polymorphism screening of IGF-I and prolactin genes in Nelore heifers
Massolini Laureano, Monyka Marianna
Beef cattle
Molecular marker
RFLP
Sexual precocity
title_short Characterization and polymorphism screening of IGF-I and prolactin genes in Nelore heifers
title_full Characterization and polymorphism screening of IGF-I and prolactin genes in Nelore heifers
title_fullStr Characterization and polymorphism screening of IGF-I and prolactin genes in Nelore heifers
title_full_unstemmed Characterization and polymorphism screening of IGF-I and prolactin genes in Nelore heifers
title_sort Characterization and polymorphism screening of IGF-I and prolactin genes in Nelore heifers
author Massolini Laureano, Monyka Marianna
author_facet Massolini Laureano, Monyka Marianna
Otaviano, Antonio Roberto
Ferreira Lima, Andre Luis
Costa, Raphael Bermal
Dias Salman, Ana Karina
Desiderio Sena, Janete Apparecida [UNESP]
Tonhati, Humberto [UNESP]
Albuquerque, Lucia Galvão de [UNESP]
author_role author
author2 Otaviano, Antonio Roberto
Ferreira Lima, Andre Luis
Costa, Raphael Bermal
Dias Salman, Ana Karina
Desiderio Sena, Janete Apparecida [UNESP]
Tonhati, Humberto [UNESP]
Albuquerque, Lucia Galvão de [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Massolini Laureano, Monyka Marianna
Otaviano, Antonio Roberto
Ferreira Lima, Andre Luis
Costa, Raphael Bermal
Dias Salman, Ana Karina
Desiderio Sena, Janete Apparecida [UNESP]
Tonhati, Humberto [UNESP]
Albuquerque, Lucia Galvão de [UNESP]
dc.subject.por.fl_str_mv Beef cattle
Molecular marker
RFLP
Sexual precocity
topic Beef cattle
Molecular marker
RFLP
Sexual precocity
description Insulin growth factor I (IGF-I) and prolactin (PRL) are peptide hormones that exert complementary effects on reproductive traits by acting on folliculogenesis. In view of the lack of information about the IGF-I and PRL genes in Bos indicus, the objective of this study was to partially characterize the promoter regions of these genes and to screen animals of different ages at first pregnancy for the presence of polymorphisms in these regions. In addition, we determined whether polymorphisms influence the regulation of the two hormone genes, evaluating their association with sexual precocity.The animals were divided into three groups according to age at first pregnancy: 1) 100 heifers considered to be sexually precocious that became pregnant at 15-16 months of age, 2) 100 heifers that became pregnant during the normal breeding season at 24 months of age, and 3) 100 heifers that did not become pregnant until 24 months of age. For the IGF-I gene, PCR-RFLP-SnaBI analysis showed the presence of genotypes AB and BB at frequencies of 0.02 and 0.98, respectively. Sequencing of the IGF-I gene fragment revealed a single nitrogen base change from cytosine to thymine, corresponding to the restriction site of SnaBI. The polymorphisms identified in the 5'-flanking region of the IGF-I gene may serve as a basis for future studies of molecular markers in cattle. For the PRL gene, PCR-RFLP-HaeIII analysis showed the presence of only one migration pattern, a finding characterizing the region studied as monomorphic. The study of other regions in the IGF-I and PRL genes might provide molecular data that can be used in the future for the selection of sexually precocious animals.
publishDate 2009
dc.date.none.fl_str_mv 2009-04-01
2014-05-20T13:13:12Z
2014-05-20T13:13:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.aspajournal.it/index.php/ijas/article/view/35
Italian Journal of Animal Science. Reggio Emilia: Assoc Sci Produzione Animale-aspa, v. 8, n. 2, p. 277-283, 2009.
1594-4077
http://hdl.handle.net/11449/1063
WOS:000268338200014
WOS000268338200014.pdf
7445254960858159
5866981114947883
url http://www.aspajournal.it/index.php/ijas/article/view/35
http://hdl.handle.net/11449/1063
identifier_str_mv Italian Journal of Animal Science. Reggio Emilia: Assoc Sci Produzione Animale-aspa, v. 8, n. 2, p. 277-283, 2009.
1594-4077
WOS:000268338200014
WOS000268338200014.pdf
7445254960858159
5866981114947883
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Italian Journal of Animal Science
0.990
0,434
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 277-283
application/pdf
dc.publisher.none.fl_str_mv Assoc Sci Produzione Animale-aspa
publisher.none.fl_str_mv Assoc Sci Produzione Animale-aspa
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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