Effect of dietary iron supplementation on the equine fecal microbiome
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/245616 |
Resumo: | Iron is an essential element for all living organisms, including bacteria, as several virulence factors and replication components are influenced by iron concentration. The objective of this study was to determine whether the composition and diversity of the fecal microbiota of adult horses are affected by supplemental dietary iron. Ten clinically healthy horses were randomly divided into a control and an iron-supplemented group (n = 5). The treated group was supplemented with oral ferrous sulphate monohydrate (720 ppm of iron), whereas the control group received 320 ppm of iron daily for 15 d. Fecal samples were collected before and 5, 10, 15, and 30 d after supplementation and frozen at 280 degrees C. DNA was sequenced using an Illumina MiSeq platform and data were analyzed using the software Mothur and linear discriminant analysis (LDA) effect size (LEfSe). Iron supplementation caused no change in the overall composition of the fecal microbiota, but some minor changes were observed in the low-abundant bacteria, as well as an increased alpha diversity after 15 d of supplementation. Significant differences in community composition of the fecal microbiota over time were observed in both groups, highlighting the importance of a control group, as there are variables that cannot be controlled in microbiome studies. |
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Effect of dietary iron supplementation on the equine fecal microbiomeIron is an essential element for all living organisms, including bacteria, as several virulence factors and replication components are influenced by iron concentration. The objective of this study was to determine whether the composition and diversity of the fecal microbiota of adult horses are affected by supplemental dietary iron. Ten clinically healthy horses were randomly divided into a control and an iron-supplemented group (n = 5). The treated group was supplemented with oral ferrous sulphate monohydrate (720 ppm of iron), whereas the control group received 320 ppm of iron daily for 15 d. Fecal samples were collected before and 5, 10, 15, and 30 d after supplementation and frozen at 280 degrees C. DNA was sequenced using an Illumina MiSeq platform and data were analyzed using the software Mothur and linear discriminant analysis (LDA) effect size (LEfSe). Iron supplementation caused no change in the overall composition of the fecal microbiota, but some minor changes were observed in the low-abundant bacteria, as well as an increased alpha diversity after 15 d of supplementation. Significant differences in community composition of the fecal microbiota over time were observed in both groups, highlighting the importance of a control group, as there are variables that cannot be controlled in microbiome studies.Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP, BrazilSao Paulo State Univ, Coll Vet Med & Anim Sci, Dept Vet Clin Sci, Botucatu, SP, BrazilUniv Guelph, Ontario Vet Coll, Dept Clin Studies, Guelph, ON, CanadaMcMaster Univ, Farncombe Family Digest Hlth Res Inst, Dept Med, Hamilton, ON, CanadaUniv Montreal, Vet Med Fac, Dept Biomed, St Hyacinthe, PQ, CanadaSao Paulo State Univ, Coll Vet Med & Anim Sci, Dept Vet Clin Sci, Botucatu, SP, BrazilCanadian Vet Med AssocUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Univ GuelphMcMaster UnivUniv MontrealArantes, Julia AssisBorges, Alexandre Secorun [UNESP]Zakia, Luiza StachewskiSurette, Michael GordonWeese, Jeffrey ScottCosta, Marcio CarvalhoArroyo, Luis Guillermo2023-07-29T12:00:10Z2023-07-29T12:00:10Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article97-104Canadian Journal of Veterinary Research-revue Canadienne de Recherche Veterinaire. Ottawa: Canadian Vet Med Assoc, v. 87, n. 2, p. 97-104, 2023.http://hdl.handle.net/11449/245616WOS:000968665800002Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCanadian Journal Of Veterinary Research-revue Canadienne De Recherche Veterinaireinfo:eu-repo/semantics/openAccess2023-07-29T12:00:10Zoai:repositorio.unesp.br:11449/245616Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T12:00:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of dietary iron supplementation on the equine fecal microbiome |
title |
Effect of dietary iron supplementation on the equine fecal microbiome |
spellingShingle |
Effect of dietary iron supplementation on the equine fecal microbiome Arantes, Julia Assis |
title_short |
Effect of dietary iron supplementation on the equine fecal microbiome |
title_full |
Effect of dietary iron supplementation on the equine fecal microbiome |
title_fullStr |
Effect of dietary iron supplementation on the equine fecal microbiome |
title_full_unstemmed |
Effect of dietary iron supplementation on the equine fecal microbiome |
title_sort |
Effect of dietary iron supplementation on the equine fecal microbiome |
author |
Arantes, Julia Assis |
author_facet |
Arantes, Julia Assis Borges, Alexandre Secorun [UNESP] Zakia, Luiza Stachewski Surette, Michael Gordon Weese, Jeffrey Scott Costa, Marcio Carvalho Arroyo, Luis Guillermo |
author_role |
author |
author2 |
Borges, Alexandre Secorun [UNESP] Zakia, Luiza Stachewski Surette, Michael Gordon Weese, Jeffrey Scott Costa, Marcio Carvalho Arroyo, Luis Guillermo |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Univ Guelph McMaster Univ Univ Montreal |
dc.contributor.author.fl_str_mv |
Arantes, Julia Assis Borges, Alexandre Secorun [UNESP] Zakia, Luiza Stachewski Surette, Michael Gordon Weese, Jeffrey Scott Costa, Marcio Carvalho Arroyo, Luis Guillermo |
description |
Iron is an essential element for all living organisms, including bacteria, as several virulence factors and replication components are influenced by iron concentration. The objective of this study was to determine whether the composition and diversity of the fecal microbiota of adult horses are affected by supplemental dietary iron. Ten clinically healthy horses were randomly divided into a control and an iron-supplemented group (n = 5). The treated group was supplemented with oral ferrous sulphate monohydrate (720 ppm of iron), whereas the control group received 320 ppm of iron daily for 15 d. Fecal samples were collected before and 5, 10, 15, and 30 d after supplementation and frozen at 280 degrees C. DNA was sequenced using an Illumina MiSeq platform and data were analyzed using the software Mothur and linear discriminant analysis (LDA) effect size (LEfSe). Iron supplementation caused no change in the overall composition of the fecal microbiota, but some minor changes were observed in the low-abundant bacteria, as well as an increased alpha diversity after 15 d of supplementation. Significant differences in community composition of the fecal microbiota over time were observed in both groups, highlighting the importance of a control group, as there are variables that cannot be controlled in microbiome studies. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:00:10Z 2023-07-29T12:00:10Z 2023-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Canadian Journal of Veterinary Research-revue Canadienne de Recherche Veterinaire. Ottawa: Canadian Vet Med Assoc, v. 87, n. 2, p. 97-104, 2023. http://hdl.handle.net/11449/245616 WOS:000968665800002 |
identifier_str_mv |
Canadian Journal of Veterinary Research-revue Canadienne de Recherche Veterinaire. Ottawa: Canadian Vet Med Assoc, v. 87, n. 2, p. 97-104, 2023. WOS:000968665800002 |
url |
http://hdl.handle.net/11449/245616 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Canadian Journal Of Veterinary Research-revue Canadienne De Recherche Veterinaire |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
97-104 |
dc.publisher.none.fl_str_mv |
Canadian Vet Med Assoc |
publisher.none.fl_str_mv |
Canadian Vet Med Assoc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790364526968832 |