Variant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ygeno.2021.11.008 http://hdl.handle.net/11449/229918 |
Resumo: | Nile tilapia (Oreochromis niloticus) is a species of worldwide importance for aquaculture. A crossbred lineage was developed through introgressive backcross breeding techniques and combines the high growth performance of the Chitralada (CHIT) lwith attractive reddish color of the Red Stirling (REDS) strains. Since the crossbreed has an unknown genetically improved background, the objective of this work was to characterize expression signatures that portray the advantageous phenotype of the crossbreeds. We characterized the microRNA transcriptome by high throughput sequencing (RNA-seq) and the proteome through mass spectrometry (ESI-Q-TOF-MS) and applied bioinformatics for the comparative analysis of such molecular data on the three strains. Crossbreed expressed a distinct set of miRNAs and proteins compared to the parents. They comprised several microRNAs regulate traits of economic interest. Proteomic profiles revealed differences between parental and crossbreed in expression of proteins associated with glycolisis. Distinctive miRNA and protein signatures contribute to the phenotype of crossbreed. |
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Variant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus)ESI-Q-TOFGrowthmiRNANile tilapiaProteomeRNA-SeqNile tilapia (Oreochromis niloticus) is a species of worldwide importance for aquaculture. A crossbred lineage was developed through introgressive backcross breeding techniques and combines the high growth performance of the Chitralada (CHIT) lwith attractive reddish color of the Red Stirling (REDS) strains. Since the crossbreed has an unknown genetically improved background, the objective of this work was to characterize expression signatures that portray the advantageous phenotype of the crossbreeds. We characterized the microRNA transcriptome by high throughput sequencing (RNA-seq) and the proteome through mass spectrometry (ESI-Q-TOF-MS) and applied bioinformatics for the comparative analysis of such molecular data on the three strains. Crossbreed expressed a distinct set of miRNAs and proteins compared to the parents. They comprised several microRNAs regulate traits of economic interest. Proteomic profiles revealed differences between parental and crossbreed in expression of proteins associated with glycolisis. Distinctive miRNA and protein signatures contribute to the phenotype of crossbreed.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Chemical and Biological Sciences Institute of Biosciences of Botucatu São Paulo State University (UNESP)Department of Biophysics and Pharmacology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Division Molecular Biotechnology and Functional Genomics Technical University of Applied Sciences WildauCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)Department of Structural and Functional Biology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Unit of Biotechnology University of Mogi das CruzesDepartment of Animal Sciences Federal University of LavrasDepartment of Chemical and Biological Sciences Institute of Biosciences of Botucatu São Paulo State University (UNESP)Department of Biophysics and Pharmacology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)Department of Structural and Functional Biology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Universidade Estadual Paulista (UNESP)Technical University of Applied Sciences WildauUniversity of Mogi das CruzesFederal University of LavrasHerkenhoff, Marcos Edgar [UNESP]Bovolenta, Luiz A. [UNESP]Broedel, Oliverdos Santos, Lucilene D. [UNESP]de Oliveira, Arthur C. [UNESP]Chuffa, Luiz G.A. [UNESP]Ribeiro, Amanda de O. [UNESP]Lupi, Luiz A. [UNESP]Dias, Marco A.D.Hilsdorf, Alexandre W.S.Frohme, MarcusPinhal, Danillo [UNESP]2022-04-29T08:36:39Z2022-04-29T08:36:39Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4303-4312http://dx.doi.org/10.1016/j.ygeno.2021.11.008Genomics, v. 113, n. 6, p. 4303-4312, 2021.1089-86460888-7543http://hdl.handle.net/11449/22991810.1016/j.ygeno.2021.11.0082-s2.0-85119451126Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenomicsinfo:eu-repo/semantics/openAccess2022-04-29T08:36:40Zoai:repositorio.unesp.br:11449/229918Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:13:34.360634Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Variant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus) |
title |
Variant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus) |
spellingShingle |
Variant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus) Herkenhoff, Marcos Edgar [UNESP] ESI-Q-TOF Growth miRNA Nile tilapia Proteome RNA-Seq |
title_short |
Variant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus) |
title_full |
Variant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus) |
title_fullStr |
Variant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus) |
title_full_unstemmed |
Variant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus) |
title_sort |
Variant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus) |
author |
Herkenhoff, Marcos Edgar [UNESP] |
author_facet |
Herkenhoff, Marcos Edgar [UNESP] Bovolenta, Luiz A. [UNESP] Broedel, Oliver dos Santos, Lucilene D. [UNESP] de Oliveira, Arthur C. [UNESP] Chuffa, Luiz G.A. [UNESP] Ribeiro, Amanda de O. [UNESP] Lupi, Luiz A. [UNESP] Dias, Marco A.D. Hilsdorf, Alexandre W.S. Frohme, Marcus Pinhal, Danillo [UNESP] |
author_role |
author |
author2 |
Bovolenta, Luiz A. [UNESP] Broedel, Oliver dos Santos, Lucilene D. [UNESP] de Oliveira, Arthur C. [UNESP] Chuffa, Luiz G.A. [UNESP] Ribeiro, Amanda de O. [UNESP] Lupi, Luiz A. [UNESP] Dias, Marco A.D. Hilsdorf, Alexandre W.S. Frohme, Marcus Pinhal, Danillo [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Technical University of Applied Sciences Wildau University of Mogi das Cruzes Federal University of Lavras |
dc.contributor.author.fl_str_mv |
Herkenhoff, Marcos Edgar [UNESP] Bovolenta, Luiz A. [UNESP] Broedel, Oliver dos Santos, Lucilene D. [UNESP] de Oliveira, Arthur C. [UNESP] Chuffa, Luiz G.A. [UNESP] Ribeiro, Amanda de O. [UNESP] Lupi, Luiz A. [UNESP] Dias, Marco A.D. Hilsdorf, Alexandre W.S. Frohme, Marcus Pinhal, Danillo [UNESP] |
dc.subject.por.fl_str_mv |
ESI-Q-TOF Growth miRNA Nile tilapia Proteome RNA-Seq |
topic |
ESI-Q-TOF Growth miRNA Nile tilapia Proteome RNA-Seq |
description |
Nile tilapia (Oreochromis niloticus) is a species of worldwide importance for aquaculture. A crossbred lineage was developed through introgressive backcross breeding techniques and combines the high growth performance of the Chitralada (CHIT) lwith attractive reddish color of the Red Stirling (REDS) strains. Since the crossbreed has an unknown genetically improved background, the objective of this work was to characterize expression signatures that portray the advantageous phenotype of the crossbreeds. We characterized the microRNA transcriptome by high throughput sequencing (RNA-seq) and the proteome through mass spectrometry (ESI-Q-TOF-MS) and applied bioinformatics for the comparative analysis of such molecular data on the three strains. Crossbreed expressed a distinct set of miRNAs and proteins compared to the parents. They comprised several microRNAs regulate traits of economic interest. Proteomic profiles revealed differences between parental and crossbreed in expression of proteins associated with glycolisis. Distinctive miRNA and protein signatures contribute to the phenotype of crossbreed. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-01 2022-04-29T08:36:39Z 2022-04-29T08:36:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ygeno.2021.11.008 Genomics, v. 113, n. 6, p. 4303-4312, 2021. 1089-8646 0888-7543 http://hdl.handle.net/11449/229918 10.1016/j.ygeno.2021.11.008 2-s2.0-85119451126 |
url |
http://dx.doi.org/10.1016/j.ygeno.2021.11.008 http://hdl.handle.net/11449/229918 |
identifier_str_mv |
Genomics, v. 113, n. 6, p. 4303-4312, 2021. 1089-8646 0888-7543 10.1016/j.ygeno.2021.11.008 2-s2.0-85119451126 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genomics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4303-4312 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128333256327168 |