Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0177679 http://hdl.handle.net/11449/162800 |
Resumo: | Skeletal muscle is capable of phenotypic adaptation to environmental factors, such as nutrient availability, by altering the balance between muscle catabolism and anabolism that in turn coordinates muscle growth. Small noncoding RNAs, known as microRNAs (miRNAs), repress the expression of target mRNAs, and many studies have demonstrated that miRNAs regulate the mRNAs of catabolic and anabolic genes. We evaluated muscle morphology, gene expression of components involved in catabolism, anabolism and energetic metabolism and miRNAs expression in both the fast and slow muscle of juvenile pacu (Piaractus mesopotamicus) during food restriction and refeeding. Our analysis revealed that short periods of food restriction followed by refeeding predominantly affected fast muscle, with changes in muscle fiber diameter and miRNAs expression. There was an increase in the mRNA levels of catabolic pathways components (FBXO25, ATG12, BCL2) and energetic metabolism-related genes (PGC1 alpha and SDHA), together with a decrease in PPAR beta/delta mRNA levels. Interestingly, an increase in mRNA levels of anabolic genes (PI3K and mTORC1 complex: mTOR, mLST8 and RAPTOR) was also observed during food restriction. After refeeding, muscle morphology showed similar patterns of the control group; the majority of genes were slightly up-or down-regulated in fast and slow muscle, respectively; the levels of all miRNAs increased in fast muscle and some of them decreased in slow muscle. Our findings demonstrated that a short period of food restriction in juvenile pacu had a considerable impact on fast muscle, increasing the expression of anabolic (PI3K and mTORC1 complex: mTOR, mLST8 and RAPTOR) and energetic metabolism genes. The miRNAs (miR-1, miR-206, miR-199 and miR-23a) were more expressed during refeeding and while their target genes (IGF-1, mTOR, PGC1 alpha and MAFbx), presented a decreased expression. The alterations in mTORC1 complex observed during fasting may have influenced the rates of protein synthesis by using amino acids from protein degradation as an alternative mechanism to preserve muscle phenotype and metabolic demand maintenance. |
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Repositório Institucional da UNESP |
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Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus)Skeletal muscle is capable of phenotypic adaptation to environmental factors, such as nutrient availability, by altering the balance between muscle catabolism and anabolism that in turn coordinates muscle growth. Small noncoding RNAs, known as microRNAs (miRNAs), repress the expression of target mRNAs, and many studies have demonstrated that miRNAs regulate the mRNAs of catabolic and anabolic genes. We evaluated muscle morphology, gene expression of components involved in catabolism, anabolism and energetic metabolism and miRNAs expression in both the fast and slow muscle of juvenile pacu (Piaractus mesopotamicus) during food restriction and refeeding. Our analysis revealed that short periods of food restriction followed by refeeding predominantly affected fast muscle, with changes in muscle fiber diameter and miRNAs expression. There was an increase in the mRNA levels of catabolic pathways components (FBXO25, ATG12, BCL2) and energetic metabolism-related genes (PGC1 alpha and SDHA), together with a decrease in PPAR beta/delta mRNA levels. Interestingly, an increase in mRNA levels of anabolic genes (PI3K and mTORC1 complex: mTOR, mLST8 and RAPTOR) was also observed during food restriction. After refeeding, muscle morphology showed similar patterns of the control group; the majority of genes were slightly up-or down-regulated in fast and slow muscle, respectively; the levels of all miRNAs increased in fast muscle and some of them decreased in slow muscle. Our findings demonstrated that a short period of food restriction in juvenile pacu had a considerable impact on fast muscle, increasing the expression of anabolic (PI3K and mTORC1 complex: mTOR, mLST8 and RAPTOR) and energetic metabolism genes. The miRNAs (miR-1, miR-206, miR-199 and miR-23a) were more expressed during refeeding and while their target genes (IGF-1, mTOR, PGC1 alpha and MAFbx), presented a decreased expression. The alterations in mTORC1 complex observed during fasting may have influenced the rates of protein synthesis by using amino acids from protein degradation as an alternative mechanism to preserve muscle phenotype and metabolic demand maintenance.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, Botucatu, SP, BrazilSao Paulo State Univ, Aquaculture Ctr, Jaboticabal, SP, BrazilSao Paulo State Univ, Inst Biosci Botucatu, Dept Biostat, Botucatu, SP, BrazilSao Paulo Agcy Agribusiness Technol, Presidente Prudente, SP, BrazilUniv Western Sao Paulo UNOESTE, Presidente Prudente, SP, BrazilSao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, Botucatu, SP, BrazilSao Paulo State Univ, Aquaculture Ctr, Jaboticabal, SP, BrazilSao Paulo State Univ, Inst Biosci Botucatu, Dept Biostat, Botucatu, SP, BrazilFAPESP: 2013/25915-1FAPESP: 2014/16949-2FAPESP: 2016/00725-3CNPq: 447233/2014CNPq: 302656/2015-4Public Library ScienceUniversidade Estadual Paulista (Unesp)Sao Paulo Agcy Agribusiness TechnolUniv Western Sao Paulo UNOESTEPaula, Tassiana Gutierrez de [UNESP]Thomazini Zanella, Bruna Tereza [UNESP]Almeida Fantinatti, Bruno Evaristo de [UNESP]Moraes, Leonardo Nazario de [UNESP]Silva Duran, Bruno Oliveira da [UNESP]Oliveira, Caroline Bredariol de [UNESP]Simoes Salomao, Rondinelle Artur [UNESP]Silva, Rafaela Nunes da [UNESP]Padovani, Carlos Roberto [UNESP]Santos, Vander Bruno dosMareco, Edson AssuncaoCarvalho, Robson Francisco [UNESP]Dal-Pai-Silva, Maeli [UNESP]2018-11-26T17:31:26Z2018-11-26T17:31:26Z2017-05-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20application/pdfhttp://dx.doi.org/10.1371/journal.pone.0177679Plos One. San Francisco: Public Library Science, v. 12, n. 5, 20 p., 2017.1932-6203http://hdl.handle.net/11449/16280010.1371/journal.pone.0177679WOS:000401473500032WOS000401473500032.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One1,164info:eu-repo/semantics/openAccess2023-11-04T06:13:00Zoai:repositorio.unesp.br:11449/162800Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-04T06:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus) |
title |
Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus) |
spellingShingle |
Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus) Paula, Tassiana Gutierrez de [UNESP] |
title_short |
Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus) |
title_full |
Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus) |
title_fullStr |
Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus) |
title_full_unstemmed |
Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus) |
title_sort |
Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus) |
author |
Paula, Tassiana Gutierrez de [UNESP] |
author_facet |
Paula, Tassiana Gutierrez de [UNESP] Thomazini Zanella, Bruna Tereza [UNESP] Almeida Fantinatti, Bruno Evaristo de [UNESP] Moraes, Leonardo Nazario de [UNESP] Silva Duran, Bruno Oliveira da [UNESP] Oliveira, Caroline Bredariol de [UNESP] Simoes Salomao, Rondinelle Artur [UNESP] Silva, Rafaela Nunes da [UNESP] Padovani, Carlos Roberto [UNESP] Santos, Vander Bruno dos Mareco, Edson Assuncao Carvalho, Robson Francisco [UNESP] Dal-Pai-Silva, Maeli [UNESP] |
author_role |
author |
author2 |
Thomazini Zanella, Bruna Tereza [UNESP] Almeida Fantinatti, Bruno Evaristo de [UNESP] Moraes, Leonardo Nazario de [UNESP] Silva Duran, Bruno Oliveira da [UNESP] Oliveira, Caroline Bredariol de [UNESP] Simoes Salomao, Rondinelle Artur [UNESP] Silva, Rafaela Nunes da [UNESP] Padovani, Carlos Roberto [UNESP] Santos, Vander Bruno dos Mareco, Edson Assuncao Carvalho, Robson Francisco [UNESP] Dal-Pai-Silva, Maeli [UNESP] |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Sao Paulo Agcy Agribusiness Technol Univ Western Sao Paulo UNOESTE |
dc.contributor.author.fl_str_mv |
Paula, Tassiana Gutierrez de [UNESP] Thomazini Zanella, Bruna Tereza [UNESP] Almeida Fantinatti, Bruno Evaristo de [UNESP] Moraes, Leonardo Nazario de [UNESP] Silva Duran, Bruno Oliveira da [UNESP] Oliveira, Caroline Bredariol de [UNESP] Simoes Salomao, Rondinelle Artur [UNESP] Silva, Rafaela Nunes da [UNESP] Padovani, Carlos Roberto [UNESP] Santos, Vander Bruno dos Mareco, Edson Assuncao Carvalho, Robson Francisco [UNESP] Dal-Pai-Silva, Maeli [UNESP] |
description |
Skeletal muscle is capable of phenotypic adaptation to environmental factors, such as nutrient availability, by altering the balance between muscle catabolism and anabolism that in turn coordinates muscle growth. Small noncoding RNAs, known as microRNAs (miRNAs), repress the expression of target mRNAs, and many studies have demonstrated that miRNAs regulate the mRNAs of catabolic and anabolic genes. We evaluated muscle morphology, gene expression of components involved in catabolism, anabolism and energetic metabolism and miRNAs expression in both the fast and slow muscle of juvenile pacu (Piaractus mesopotamicus) during food restriction and refeeding. Our analysis revealed that short periods of food restriction followed by refeeding predominantly affected fast muscle, with changes in muscle fiber diameter and miRNAs expression. There was an increase in the mRNA levels of catabolic pathways components (FBXO25, ATG12, BCL2) and energetic metabolism-related genes (PGC1 alpha and SDHA), together with a decrease in PPAR beta/delta mRNA levels. Interestingly, an increase in mRNA levels of anabolic genes (PI3K and mTORC1 complex: mTOR, mLST8 and RAPTOR) was also observed during food restriction. After refeeding, muscle morphology showed similar patterns of the control group; the majority of genes were slightly up-or down-regulated in fast and slow muscle, respectively; the levels of all miRNAs increased in fast muscle and some of them decreased in slow muscle. Our findings demonstrated that a short period of food restriction in juvenile pacu had a considerable impact on fast muscle, increasing the expression of anabolic (PI3K and mTORC1 complex: mTOR, mLST8 and RAPTOR) and energetic metabolism genes. The miRNAs (miR-1, miR-206, miR-199 and miR-23a) were more expressed during refeeding and while their target genes (IGF-1, mTOR, PGC1 alpha and MAFbx), presented a decreased expression. The alterations in mTORC1 complex observed during fasting may have influenced the rates of protein synthesis by using amino acids from protein degradation as an alternative mechanism to preserve muscle phenotype and metabolic demand maintenance. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-15 2018-11-26T17:31:26Z 2018-11-26T17:31:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0177679 Plos One. San Francisco: Public Library Science, v. 12, n. 5, 20 p., 2017. 1932-6203 http://hdl.handle.net/11449/162800 10.1371/journal.pone.0177679 WOS:000401473500032 WOS000401473500032.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0177679 http://hdl.handle.net/11449/162800 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 12, n. 5, 20 p., 2017. 1932-6203 10.1371/journal.pone.0177679 WOS:000401473500032 WOS000401473500032.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
20 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964820086194176 |