In Vitro Development and Mitochondrial Gene Expression in Brown Brocket Deer (Mazama gouazoubira) Embryos Obtained by Interspecific Somatic Cell Nuclear Transfer
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1089/cell.2019.0069 http://hdl.handle.net/11449/199253 |
Resumo: | The genetic diversity of Neotropical deer is increasingly jeopardized, owing to declining population size. Thus, the formation of cryobanking of somatic cells is important for the preservation of these species using cloning. The transformation of these cells into viable embryos has been hampered by a lack of endangered species oocytes. Accordingly, the aim of this study was to produce brown brocket deer embryos by interspecific somatic cell nuclear transfer (iSCNT), using goat or cattle oocytes as cytoplasts, and to elucidate embryo mitochondrial activity by measuring the expression levels of ATP6, COX3, and ND5. Cattle embryos produced by in vitro fertilization (IVF) were used as a control. There were no differences in the development of embryos produced by traditional SCNT and iSCNT when using either the goat cytoplasts (38.4% vs. 25.0% cleaved and 40.0% vs. 50.0% morula rates, respectively) or cattle cytoplast (72.8% vs. 65.5% cleaved and 11.3% vs. 5.9% blastocyst rates, respectively). Concerning the gene expression, no significant difference was observed when goat oocytes were used as cytoplasts. However, when using cattle oocytes and 16S as a reference gene, the iSCNT upregulated COX3, when compared with SCNT group. In contrast, when GAPDH was used as a reference gene, all the evaluated genes were upregulated in the iSCNT group, when compared with the IVF group. When compared with the SCNT group, only the expression of ATP6 was statistically different. In conclusion, it was demonstrated that interspecific nuclear transfer is a potentially useful tool for conservation programs of endangered similar deer species. |
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In Vitro Development and Mitochondrial Gene Expression in Brown Brocket Deer (Mazama gouazoubira) Embryos Obtained by Interspecific Somatic Cell Nuclear Transfercloningconservationdeergene expressionmitochondrianuclear transferThe genetic diversity of Neotropical deer is increasingly jeopardized, owing to declining population size. Thus, the formation of cryobanking of somatic cells is important for the preservation of these species using cloning. The transformation of these cells into viable embryos has been hampered by a lack of endangered species oocytes. Accordingly, the aim of this study was to produce brown brocket deer embryos by interspecific somatic cell nuclear transfer (iSCNT), using goat or cattle oocytes as cytoplasts, and to elucidate embryo mitochondrial activity by measuring the expression levels of ATP6, COX3, and ND5. Cattle embryos produced by in vitro fertilization (IVF) were used as a control. There were no differences in the development of embryos produced by traditional SCNT and iSCNT when using either the goat cytoplasts (38.4% vs. 25.0% cleaved and 40.0% vs. 50.0% morula rates, respectively) or cattle cytoplast (72.8% vs. 65.5% cleaved and 11.3% vs. 5.9% blastocyst rates, respectively). Concerning the gene expression, no significant difference was observed when goat oocytes were used as cytoplasts. However, when using cattle oocytes and 16S as a reference gene, the iSCNT upregulated COX3, when compared with SCNT group. In contrast, when GAPDH was used as a reference gene, all the evaluated genes were upregulated in the iSCNT group, when compared with the IVF group. When compared with the SCNT group, only the expression of ATP6 was statistically different. In conclusion, it was demonstrated that interspecific nuclear transfer is a potentially useful tool for conservation programs of endangered similar deer species.Laboratory of Physiology Control of Reproduction Faculty of Veterinary Ceará State University (UECE)Laboratory of Animal Biotechnology National University Toribio Rodriguez de MendozaDepartment of Biology University of SaskatchewanDepartment of Animal Science Deer Research and Conservation Center São Paulo State University (UNESP)Molecular Genetics Research Unit University Center Fametro (UNIFAMETRO)Department of Animal Science Deer Research and Conservation Center São Paulo State University (UNESP)Ceará State University (UECE)National University Toribio Rodriguez de MendozaUniversity of SaskatchewanUniversidade Estadual Paulista (Unesp)University Center Fametro (UNIFAMETRO)Magalhães, Lívia C.Cortez, Jenin V.Bhat, Maajid H.Sampaio, Ana Clara N.P.C.Freitas, Jeferson L.S.Duarte, José M.B. [UNESP]Melo, Luciana M.Freitas, Vicente J.F.2020-12-12T01:34:49Z2020-12-12T01:34:49Z2020-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article208-216http://dx.doi.org/10.1089/cell.2019.0069Cellular Reprogramming, v. 22, n. 4, p. 208-216, 2020.2152-49982152-4971http://hdl.handle.net/11449/19925310.1089/cell.2019.00692-s2.0-85089404989Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellular Reprogramminginfo:eu-repo/semantics/openAccess2021-10-23T05:43:35Zoai:repositorio.unesp.br:11449/199253Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T05:43:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In Vitro Development and Mitochondrial Gene Expression in Brown Brocket Deer (Mazama gouazoubira) Embryos Obtained by Interspecific Somatic Cell Nuclear Transfer |
title |
In Vitro Development and Mitochondrial Gene Expression in Brown Brocket Deer (Mazama gouazoubira) Embryos Obtained by Interspecific Somatic Cell Nuclear Transfer |
spellingShingle |
In Vitro Development and Mitochondrial Gene Expression in Brown Brocket Deer (Mazama gouazoubira) Embryos Obtained by Interspecific Somatic Cell Nuclear Transfer Magalhães, Lívia C. cloning conservation deer gene expression mitochondria nuclear transfer |
title_short |
In Vitro Development and Mitochondrial Gene Expression in Brown Brocket Deer (Mazama gouazoubira) Embryos Obtained by Interspecific Somatic Cell Nuclear Transfer |
title_full |
In Vitro Development and Mitochondrial Gene Expression in Brown Brocket Deer (Mazama gouazoubira) Embryos Obtained by Interspecific Somatic Cell Nuclear Transfer |
title_fullStr |
In Vitro Development and Mitochondrial Gene Expression in Brown Brocket Deer (Mazama gouazoubira) Embryos Obtained by Interspecific Somatic Cell Nuclear Transfer |
title_full_unstemmed |
In Vitro Development and Mitochondrial Gene Expression in Brown Brocket Deer (Mazama gouazoubira) Embryos Obtained by Interspecific Somatic Cell Nuclear Transfer |
title_sort |
In Vitro Development and Mitochondrial Gene Expression in Brown Brocket Deer (Mazama gouazoubira) Embryos Obtained by Interspecific Somatic Cell Nuclear Transfer |
author |
Magalhães, Lívia C. |
author_facet |
Magalhães, Lívia C. Cortez, Jenin V. Bhat, Maajid H. Sampaio, Ana Clara N.P.C. Freitas, Jeferson L.S. Duarte, José M.B. [UNESP] Melo, Luciana M. Freitas, Vicente J.F. |
author_role |
author |
author2 |
Cortez, Jenin V. Bhat, Maajid H. Sampaio, Ana Clara N.P.C. Freitas, Jeferson L.S. Duarte, José M.B. [UNESP] Melo, Luciana M. Freitas, Vicente J.F. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Ceará State University (UECE) National University Toribio Rodriguez de Mendoza University of Saskatchewan Universidade Estadual Paulista (Unesp) University Center Fametro (UNIFAMETRO) |
dc.contributor.author.fl_str_mv |
Magalhães, Lívia C. Cortez, Jenin V. Bhat, Maajid H. Sampaio, Ana Clara N.P.C. Freitas, Jeferson L.S. Duarte, José M.B. [UNESP] Melo, Luciana M. Freitas, Vicente J.F. |
dc.subject.por.fl_str_mv |
cloning conservation deer gene expression mitochondria nuclear transfer |
topic |
cloning conservation deer gene expression mitochondria nuclear transfer |
description |
The genetic diversity of Neotropical deer is increasingly jeopardized, owing to declining population size. Thus, the formation of cryobanking of somatic cells is important for the preservation of these species using cloning. The transformation of these cells into viable embryos has been hampered by a lack of endangered species oocytes. Accordingly, the aim of this study was to produce brown brocket deer embryos by interspecific somatic cell nuclear transfer (iSCNT), using goat or cattle oocytes as cytoplasts, and to elucidate embryo mitochondrial activity by measuring the expression levels of ATP6, COX3, and ND5. Cattle embryos produced by in vitro fertilization (IVF) were used as a control. There were no differences in the development of embryos produced by traditional SCNT and iSCNT when using either the goat cytoplasts (38.4% vs. 25.0% cleaved and 40.0% vs. 50.0% morula rates, respectively) or cattle cytoplast (72.8% vs. 65.5% cleaved and 11.3% vs. 5.9% blastocyst rates, respectively). Concerning the gene expression, no significant difference was observed when goat oocytes were used as cytoplasts. However, when using cattle oocytes and 16S as a reference gene, the iSCNT upregulated COX3, when compared with SCNT group. In contrast, when GAPDH was used as a reference gene, all the evaluated genes were upregulated in the iSCNT group, when compared with the IVF group. When compared with the SCNT group, only the expression of ATP6 was statistically different. In conclusion, it was demonstrated that interspecific nuclear transfer is a potentially useful tool for conservation programs of endangered similar deer species. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:34:49Z 2020-12-12T01:34:49Z 2020-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1089/cell.2019.0069 Cellular Reprogramming, v. 22, n. 4, p. 208-216, 2020. 2152-4998 2152-4971 http://hdl.handle.net/11449/199253 10.1089/cell.2019.0069 2-s2.0-85089404989 |
url |
http://dx.doi.org/10.1089/cell.2019.0069 http://hdl.handle.net/11449/199253 |
identifier_str_mv |
Cellular Reprogramming, v. 22, n. 4, p. 208-216, 2020. 2152-4998 2152-4971 10.1089/cell.2019.0069 2-s2.0-85089404989 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cellular Reprogramming |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
208-216 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965683682902016 |