Chemical and morphological analysis of dentin collagen degradation after Nd:YAG laser irradiation

Detalhes bibliográficos
Autor(a) principal: Lopes, Stephanie Ribeiro [UNESP]
Data de Publicação: 2022
Outros Autores: da Silva, Tânia Mara, Maselli, Andrea [UNESP], Gonçalves, Lucélia Lemes, Campos, Tiago Moreira Bastos, Gonçalves, Sérgio Eduardo de Paiva [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s41547-022-00149-y
http://hdl.handle.net/11449/230595
Resumo: Objective: The present study aimed to evaluate root dentin collagen degradation after Nd:YAG laser irradiation by using polarized light microscopy and spectroscopy analysis. Background: Quantification of collagen degradation is an important parameter to evaluate dentin caries progression. Methods: Discs (2 mm in thickness × 6 mm in diameter) of bovine root dentin were obtained and submitted to demineralization by artificial caries lesion formation. The specimens were randomly divided into two groups according to the following treatments: C—control (no treatment) and L—Nd:YAG laser irradiation (1064 nm; 60 mJ; 10 Hz; 48 J/cm2; non-contact; 30 s). After the treatment, the specimens were exposed to the collagenase enzyme challenge for 5 days. Chemical analysis of the dentin surface was performed by FT-IR spectroscopy. Specimens were sectioned longitudinally to prepare slides for polarized light microscopy evaluation to provide the measure of depth of the degraded collagen in the demineralized area. Data were submitted to the Student’s t-test (p < 0.05). Results: According to the t-Student test (t-value = 5.089), it was observed that the Nd:YAG laser showed statistically significant differences when compared to the control group (p < 0.001). Chemical differences in dentin were observed through the reduction of amide, carbonate, and phosphate groups present in the collagen matrix after laser irradiation. Conclusions: Nd:YAG laser promoted chemical and morphological changes in the surface of the root dentin, showing effectiveness in reducing collagen degradation.
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spelling Chemical and morphological analysis of dentin collagen degradation after Nd:YAG laser irradiationCariesCollagen degradationFT-IR spectroscopyMicroscopyNd:YAG laserObjective: The present study aimed to evaluate root dentin collagen degradation after Nd:YAG laser irradiation by using polarized light microscopy and spectroscopy analysis. Background: Quantification of collagen degradation is an important parameter to evaluate dentin caries progression. Methods: Discs (2 mm in thickness × 6 mm in diameter) of bovine root dentin were obtained and submitted to demineralization by artificial caries lesion formation. The specimens were randomly divided into two groups according to the following treatments: C—control (no treatment) and L—Nd:YAG laser irradiation (1064 nm; 60 mJ; 10 Hz; 48 J/cm2; non-contact; 30 s). After the treatment, the specimens were exposed to the collagenase enzyme challenge for 5 days. Chemical analysis of the dentin surface was performed by FT-IR spectroscopy. Specimens were sectioned longitudinally to prepare slides for polarized light microscopy evaluation to provide the measure of depth of the degraded collagen in the demineralized area. Data were submitted to the Student’s t-test (p < 0.05). Results: According to the t-Student test (t-value = 5.089), it was observed that the Nd:YAG laser showed statistically significant differences when compared to the control group (p < 0.001). Chemical differences in dentin were observed through the reduction of amide, carbonate, and phosphate groups present in the collagen matrix after laser irradiation. Conclusions: Nd:YAG laser promoted chemical and morphological changes in the surface of the root dentin, showing effectiveness in reducing collagen degradation.Department of Restorative Dentistry Institute of Science and Technology of São José dos Campos Sao Paulo State University (UNESP), Av. Engenheiro Francisco José Longo, 777, Jardim São Dimas, São José dos CamposDepartment of Restorative Dentistry Anhanguera University of São Paulo, São José dos CamposDepartment of Physics Technological Institute of Aeronautics (ITA), São José dos CamposDepartment of Restorative Dentistry Institute of Science and Technology of São José dos Campos Sao Paulo State University (UNESP), Av. Engenheiro Francisco José Longo, 777, Jardim São Dimas, São José dos CamposUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Technological Institute of Aeronautics (ITA)Lopes, Stephanie Ribeiro [UNESP]da Silva, Tânia MaraMaselli, Andrea [UNESP]Gonçalves, Lucélia LemesCampos, Tiago Moreira BastosGonçalves, Sérgio Eduardo de Paiva [UNESP]2022-04-29T08:40:54Z2022-04-29T08:40:54Z2022-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article47-53http://dx.doi.org/10.1007/s41547-022-00149-yLasers in Dental Science, v. 6, n. 1, p. 47-53, 2022.2367-2587http://hdl.handle.net/11449/23059510.1007/s41547-022-00149-y2-s2.0-85126564107Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Dental Scienceinfo:eu-repo/semantics/openAccess2022-04-29T08:40:54Zoai:repositorio.unesp.br:11449/230595Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:54:18.065391Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Chemical and morphological analysis of dentin collagen degradation after Nd:YAG laser irradiation
title Chemical and morphological analysis of dentin collagen degradation after Nd:YAG laser irradiation
spellingShingle Chemical and morphological analysis of dentin collagen degradation after Nd:YAG laser irradiation
Lopes, Stephanie Ribeiro [UNESP]
Caries
Collagen degradation
FT-IR spectroscopy
Microscopy
Nd:YAG laser
title_short Chemical and morphological analysis of dentin collagen degradation after Nd:YAG laser irradiation
title_full Chemical and morphological analysis of dentin collagen degradation after Nd:YAG laser irradiation
title_fullStr Chemical and morphological analysis of dentin collagen degradation after Nd:YAG laser irradiation
title_full_unstemmed Chemical and morphological analysis of dentin collagen degradation after Nd:YAG laser irradiation
title_sort Chemical and morphological analysis of dentin collagen degradation after Nd:YAG laser irradiation
author Lopes, Stephanie Ribeiro [UNESP]
author_facet Lopes, Stephanie Ribeiro [UNESP]
da Silva, Tânia Mara
Maselli, Andrea [UNESP]
Gonçalves, Lucélia Lemes
Campos, Tiago Moreira Bastos
Gonçalves, Sérgio Eduardo de Paiva [UNESP]
author_role author
author2 da Silva, Tânia Mara
Maselli, Andrea [UNESP]
Gonçalves, Lucélia Lemes
Campos, Tiago Moreira Bastos
Gonçalves, Sérgio Eduardo de Paiva [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
Technological Institute of Aeronautics (ITA)
dc.contributor.author.fl_str_mv Lopes, Stephanie Ribeiro [UNESP]
da Silva, Tânia Mara
Maselli, Andrea [UNESP]
Gonçalves, Lucélia Lemes
Campos, Tiago Moreira Bastos
Gonçalves, Sérgio Eduardo de Paiva [UNESP]
dc.subject.por.fl_str_mv Caries
Collagen degradation
FT-IR spectroscopy
Microscopy
Nd:YAG laser
topic Caries
Collagen degradation
FT-IR spectroscopy
Microscopy
Nd:YAG laser
description Objective: The present study aimed to evaluate root dentin collagen degradation after Nd:YAG laser irradiation by using polarized light microscopy and spectroscopy analysis. Background: Quantification of collagen degradation is an important parameter to evaluate dentin caries progression. Methods: Discs (2 mm in thickness × 6 mm in diameter) of bovine root dentin were obtained and submitted to demineralization by artificial caries lesion formation. The specimens were randomly divided into two groups according to the following treatments: C—control (no treatment) and L—Nd:YAG laser irradiation (1064 nm; 60 mJ; 10 Hz; 48 J/cm2; non-contact; 30 s). After the treatment, the specimens were exposed to the collagenase enzyme challenge for 5 days. Chemical analysis of the dentin surface was performed by FT-IR spectroscopy. Specimens were sectioned longitudinally to prepare slides for polarized light microscopy evaluation to provide the measure of depth of the degraded collagen in the demineralized area. Data were submitted to the Student’s t-test (p < 0.05). Results: According to the t-Student test (t-value = 5.089), it was observed that the Nd:YAG laser showed statistically significant differences when compared to the control group (p < 0.001). Chemical differences in dentin were observed through the reduction of amide, carbonate, and phosphate groups present in the collagen matrix after laser irradiation. Conclusions: Nd:YAG laser promoted chemical and morphological changes in the surface of the root dentin, showing effectiveness in reducing collagen degradation.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:40:54Z
2022-04-29T08:40:54Z
2022-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s41547-022-00149-y
Lasers in Dental Science, v. 6, n. 1, p. 47-53, 2022.
2367-2587
http://hdl.handle.net/11449/230595
10.1007/s41547-022-00149-y
2-s2.0-85126564107
url http://dx.doi.org/10.1007/s41547-022-00149-y
http://hdl.handle.net/11449/230595
identifier_str_mv Lasers in Dental Science, v. 6, n. 1, p. 47-53, 2022.
2367-2587
10.1007/s41547-022-00149-y
2-s2.0-85126564107
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lasers in Dental Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 47-53
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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