Placental scaffolds have the ability to support adipose-derived cells differentiation into osteogenic and chondrogenic lineages

Detalhes bibliográficos
Autor(a) principal: Baracho Trindade Hill, Amanda [UNESP]
Data de Publicação: 2020
Outros Autores: Speri Alves, Antonio Alexandre, Silva Nunes Barreto, Rodrigo da, Fernandes Bressan, Fabiana, Miglino, Maria Angelica, Mansano Garcia, Joaquim [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/term.3124
http://hdl.handle.net/11449/209452
Resumo: Prudent choices of cell sources and biomaterials, as well as meticulous cultivation of the tissue microenvironment, are essential to improving outcomes of tissue engineering treatments. With the goal of providing a high-quality alternative for bone and cartilage tissue engineering, we investigated the capability of bovine placental scaffolds to support adipose-derived cell differentiation into osteogenic and chondrogenic lineages. Decellularized bovine placenta, a high-quality scaffold with practical scalability, was chosen as the biomaterial due to its rich extracellular matrix, well-developed vasculature, high availability, low cost, and simplicity of collection. Adipose-derived cells were chosen as the cell source as they are easy to isolate, nontumorigenic, and flexibly differentiable. The bovine model was chosen for its advantages in translational medicine over the mouse model. When seeded onto the scaffolds, the isolated cells adhered to the scaffolds with cell projections, established cell-scaffold communication and proliferated while maintaining cell-cell communication. Throughout a 21-day culture period, osteogenically differentiated cells secreted mineralized matrix, and calcium deposits were observed throughout the scaffold. Under chondrogenic specific differentiation conditions, the cells modified their morphology from fibroblast-like to round cells and cartilage lacunas were observed as well as the deposit of cartilaginous matrix on the placental scaffolds. This experiment provides evidence, for the first time, that bovine placental scaffolds have the potential to support bovine mesenchymal stem cell adherence and differentiation into osteogenic and chondrogenic lineages. Therefore, the constructed material could be used for bone and cartilage tissue engineering.
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spelling Placental scaffolds have the ability to support adipose-derived cells differentiation into osteogenic and chondrogenic lineagesbone tissue engineeringcartilage tissue engineeringcowsdecellularizationmesenchymal stem cellsplacentarecellularizationtranslational medicinePrudent choices of cell sources and biomaterials, as well as meticulous cultivation of the tissue microenvironment, are essential to improving outcomes of tissue engineering treatments. With the goal of providing a high-quality alternative for bone and cartilage tissue engineering, we investigated the capability of bovine placental scaffolds to support adipose-derived cell differentiation into osteogenic and chondrogenic lineages. Decellularized bovine placenta, a high-quality scaffold with practical scalability, was chosen as the biomaterial due to its rich extracellular matrix, well-developed vasculature, high availability, low cost, and simplicity of collection. Adipose-derived cells were chosen as the cell source as they are easy to isolate, nontumorigenic, and flexibly differentiable. The bovine model was chosen for its advantages in translational medicine over the mouse model. When seeded onto the scaffolds, the isolated cells adhered to the scaffolds with cell projections, established cell-scaffold communication and proliferated while maintaining cell-cell communication. Throughout a 21-day culture period, osteogenically differentiated cells secreted mineralized matrix, and calcium deposits were observed throughout the scaffold. Under chondrogenic specific differentiation conditions, the cells modified their morphology from fibroblast-like to round cells and cartilage lacunas were observed as well as the deposit of cartilaginous matrix on the placental scaffolds. This experiment provides evidence, for the first time, that bovine placental scaffolds have the potential to support bovine mesenchymal stem cell adherence and differentiation into osteogenic and chondrogenic lineages. Therefore, the constructed material could be used for bone and cartilage tissue engineering.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Montreal, Reprod & Fertil Res Ctr, St Hyacinthe, PQ, CanadaSao Paulo State Univ, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, BrazilUniv Sao Paulo, Sch Vet Med & Anim Sci, Sao Paulo, BrazilUniv Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Sao Paulo, BrazilSao Paulo State Univ, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, BrazilFAPESP: 2014/50844-3FAPESP: 2015/14535-9FAPESP: 2015/26818-5Wiley-BlackwellUniv MontrealUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Baracho Trindade Hill, Amanda [UNESP]Speri Alves, Antonio AlexandreSilva Nunes Barreto, Rodrigo daFernandes Bressan, FabianaMiglino, Maria AngelicaMansano Garcia, Joaquim [UNESP]2021-06-25T12:19:08Z2021-06-25T12:19:08Z2020-09-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1661-1672http://dx.doi.org/10.1002/term.3124Journal Of Tissue Engineering And Regenerative Medicine. Hoboken: Wiley, v. 14, n. 11, p. 1661-1672, 2020.1932-6254http://hdl.handle.net/11449/20945210.1002/term.3124WOS:000568744200001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Tissue Engineering And Regenerative Medicineinfo:eu-repo/semantics/openAccess2024-06-06T18:09:46Zoai:repositorio.unesp.br:11449/209452Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:21:39.966901Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Placental scaffolds have the ability to support adipose-derived cells differentiation into osteogenic and chondrogenic lineages
title Placental scaffolds have the ability to support adipose-derived cells differentiation into osteogenic and chondrogenic lineages
spellingShingle Placental scaffolds have the ability to support adipose-derived cells differentiation into osteogenic and chondrogenic lineages
Baracho Trindade Hill, Amanda [UNESP]
bone tissue engineering
cartilage tissue engineering
cows
decellularization
mesenchymal stem cells
placenta
recellularization
translational medicine
title_short Placental scaffolds have the ability to support adipose-derived cells differentiation into osteogenic and chondrogenic lineages
title_full Placental scaffolds have the ability to support adipose-derived cells differentiation into osteogenic and chondrogenic lineages
title_fullStr Placental scaffolds have the ability to support adipose-derived cells differentiation into osteogenic and chondrogenic lineages
title_full_unstemmed Placental scaffolds have the ability to support adipose-derived cells differentiation into osteogenic and chondrogenic lineages
title_sort Placental scaffolds have the ability to support adipose-derived cells differentiation into osteogenic and chondrogenic lineages
author Baracho Trindade Hill, Amanda [UNESP]
author_facet Baracho Trindade Hill, Amanda [UNESP]
Speri Alves, Antonio Alexandre
Silva Nunes Barreto, Rodrigo da
Fernandes Bressan, Fabiana
Miglino, Maria Angelica
Mansano Garcia, Joaquim [UNESP]
author_role author
author2 Speri Alves, Antonio Alexandre
Silva Nunes Barreto, Rodrigo da
Fernandes Bressan, Fabiana
Miglino, Maria Angelica
Mansano Garcia, Joaquim [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Montreal
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Baracho Trindade Hill, Amanda [UNESP]
Speri Alves, Antonio Alexandre
Silva Nunes Barreto, Rodrigo da
Fernandes Bressan, Fabiana
Miglino, Maria Angelica
Mansano Garcia, Joaquim [UNESP]
dc.subject.por.fl_str_mv bone tissue engineering
cartilage tissue engineering
cows
decellularization
mesenchymal stem cells
placenta
recellularization
translational medicine
topic bone tissue engineering
cartilage tissue engineering
cows
decellularization
mesenchymal stem cells
placenta
recellularization
translational medicine
description Prudent choices of cell sources and biomaterials, as well as meticulous cultivation of the tissue microenvironment, are essential to improving outcomes of tissue engineering treatments. With the goal of providing a high-quality alternative for bone and cartilage tissue engineering, we investigated the capability of bovine placental scaffolds to support adipose-derived cell differentiation into osteogenic and chondrogenic lineages. Decellularized bovine placenta, a high-quality scaffold with practical scalability, was chosen as the biomaterial due to its rich extracellular matrix, well-developed vasculature, high availability, low cost, and simplicity of collection. Adipose-derived cells were chosen as the cell source as they are easy to isolate, nontumorigenic, and flexibly differentiable. The bovine model was chosen for its advantages in translational medicine over the mouse model. When seeded onto the scaffolds, the isolated cells adhered to the scaffolds with cell projections, established cell-scaffold communication and proliferated while maintaining cell-cell communication. Throughout a 21-day culture period, osteogenically differentiated cells secreted mineralized matrix, and calcium deposits were observed throughout the scaffold. Under chondrogenic specific differentiation conditions, the cells modified their morphology from fibroblast-like to round cells and cartilage lacunas were observed as well as the deposit of cartilaginous matrix on the placental scaffolds. This experiment provides evidence, for the first time, that bovine placental scaffolds have the potential to support bovine mesenchymal stem cell adherence and differentiation into osteogenic and chondrogenic lineages. Therefore, the constructed material could be used for bone and cartilage tissue engineering.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-10
2021-06-25T12:19:08Z
2021-06-25T12:19:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/term.3124
Journal Of Tissue Engineering And Regenerative Medicine. Hoboken: Wiley, v. 14, n. 11, p. 1661-1672, 2020.
1932-6254
http://hdl.handle.net/11449/209452
10.1002/term.3124
WOS:000568744200001
url http://dx.doi.org/10.1002/term.3124
http://hdl.handle.net/11449/209452
identifier_str_mv Journal Of Tissue Engineering And Regenerative Medicine. Hoboken: Wiley, v. 14, n. 11, p. 1661-1672, 2020.
1932-6254
10.1002/term.3124
WOS:000568744200001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Tissue Engineering And Regenerative Medicine
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1661-1672
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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