Comparison of oxidative stress and the frequency of polymorphisms in the HFE gene between hemoglobin S trait blood donors and sickle cell disease patients
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4238/2011.December.8.4 http://hdl.handle.net/11449/42631 |
Resumo: | It is well documented that Hb S and iron affect blood cells, and trigger oxidative processes and generation of free radicals with potential for lipid peroxidation. We evaluated the frequency of polymorphisms in the HFE gene in Hb AS blood donors and how these polymorphisms influenced lipid peroxidation and antioxidant capacity. Blood samples were collected from 211 Hb AS blood donors, 119 Hb AA blood donors as a control group, and 28 sickle cell disease patients (Hb SS). The H63D allele was found at a frequency of 10.5% in the Hb AS samples, and the C282Y allele frequency was 0.7%. In the control group, the frequencies of the H63D and C282Y alleles were 13.4 and 2.1%, respectively. In the sickle-cell disease patients, the H63D and the C282Y allele frequencies were 10.7 and 3.5%, respectively. The frequencies of the C282Y and H63D polymorphisms in Hb AS blood donors are similar to those reported for the Brazilian population. Serum malondialdehyde values, indicative of lipid peroxidation, were highest in sickle cell patients, independent of the polymorphisms in the HFE gene, with significant differences, showing the influence of Hb S allele in the levels of lipid peroxidation. However, the trolox equivalent antioxidant capacity average levels, indicative of the antioxidant capacity, were reduced with significant differences, indicating that in spite of a lipid peroxidation raise, this is not followed by the increased of the antioxidant capacity, leading to oxidative stress. |
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Comparison of oxidative stress and the frequency of polymorphisms in the HFE gene between hemoglobin S trait blood donors and sickle cell disease patientsHemoglobinopathyHemoglobin SAntioxidant capacityHFEHemochromatosisIt is well documented that Hb S and iron affect blood cells, and trigger oxidative processes and generation of free radicals with potential for lipid peroxidation. We evaluated the frequency of polymorphisms in the HFE gene in Hb AS blood donors and how these polymorphisms influenced lipid peroxidation and antioxidant capacity. Blood samples were collected from 211 Hb AS blood donors, 119 Hb AA blood donors as a control group, and 28 sickle cell disease patients (Hb SS). The H63D allele was found at a frequency of 10.5% in the Hb AS samples, and the C282Y allele frequency was 0.7%. In the control group, the frequencies of the H63D and C282Y alleles were 13.4 and 2.1%, respectively. In the sickle-cell disease patients, the H63D and the C282Y allele frequencies were 10.7 and 3.5%, respectively. The frequencies of the C282Y and H63D polymorphisms in Hb AS blood donors are similar to those reported for the Brazilian population. Serum malondialdehyde values, indicative of lipid peroxidation, were highest in sickle cell patients, independent of the polymorphisms in the HFE gene, with significant differences, showing the influence of Hb S allele in the levels of lipid peroxidation. However, the trolox equivalent antioxidant capacity average levels, indicative of the antioxidant capacity, were reduced with significant differences, indicating that in spite of a lipid peroxidation raise, this is not followed by the increased of the antioxidant capacity, leading to oxidative stress.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual São Paulo Julio de Mesquita Filho, Inst Biociencias Letras & Ciencias Exatas, Lab Hemoglobinas & Genet Doencas Hematol, Sao Jose do Rio Preto, SP, BrazilHemoctr, Sao Jose do Rio Preto, SP, BrazilFaculdade de Medicina de São José do Rio Preto (FAMERP), Dept Biol Mol, Sao Jose do Rio Preto, SP, BrazilUniv São Paulo, Hemoctr Ribeirao Preto, BR-14049 Ribeirao Preto, SP, BrazilUniv Estadual São Paulo Julio de Mesquita Filho, Inst Biociencias Letras & Ciencias Exatas, Lab Hemoglobinas & Genet Doencas Hematol, Sao Jose do Rio Preto, SP, BrazilFunpec-editoraUniversidade Estadual Paulista (Unesp)HemoctrFaculdade de Medicina de São José do Rio Preto (FAMERP)Universidade de São Paulo (USP)Viana-Baracioli, L. M. S. [UNESP]Tukamoto Junior, N. C. [UNESP]Ricci Junior, O.Mattos, L. C.Angulo, I. L.Bonini-Domingos, C. R. [UNESP]2014-05-20T15:34:42Z2014-05-20T15:34:42Z2011-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3446-3454application/pdfhttp://dx.doi.org/10.4238/2011.December.8.4Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 10, n. 4, p. 3446-3454, 2011.1676-5680http://hdl.handle.net/11449/4263110.4238/2011.December.8.4WOS:000300617600119WOS000300617600119.pdf32794280661767190000-0002-4603-9467Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2023-10-21T06:03:48Zoai:repositorio.unesp.br:11449/42631Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:30:09.843232Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison of oxidative stress and the frequency of polymorphisms in the HFE gene between hemoglobin S trait blood donors and sickle cell disease patients |
title |
Comparison of oxidative stress and the frequency of polymorphisms in the HFE gene between hemoglobin S trait blood donors and sickle cell disease patients |
spellingShingle |
Comparison of oxidative stress and the frequency of polymorphisms in the HFE gene between hemoglobin S trait blood donors and sickle cell disease patients Viana-Baracioli, L. M. S. [UNESP] Hemoglobinopathy Hemoglobin S Antioxidant capacity HFE Hemochromatosis |
title_short |
Comparison of oxidative stress and the frequency of polymorphisms in the HFE gene between hemoglobin S trait blood donors and sickle cell disease patients |
title_full |
Comparison of oxidative stress and the frequency of polymorphisms in the HFE gene between hemoglobin S trait blood donors and sickle cell disease patients |
title_fullStr |
Comparison of oxidative stress and the frequency of polymorphisms in the HFE gene between hemoglobin S trait blood donors and sickle cell disease patients |
title_full_unstemmed |
Comparison of oxidative stress and the frequency of polymorphisms in the HFE gene between hemoglobin S trait blood donors and sickle cell disease patients |
title_sort |
Comparison of oxidative stress and the frequency of polymorphisms in the HFE gene between hemoglobin S trait blood donors and sickle cell disease patients |
author |
Viana-Baracioli, L. M. S. [UNESP] |
author_facet |
Viana-Baracioli, L. M. S. [UNESP] Tukamoto Junior, N. C. [UNESP] Ricci Junior, O. Mattos, L. C. Angulo, I. L. Bonini-Domingos, C. R. [UNESP] |
author_role |
author |
author2 |
Tukamoto Junior, N. C. [UNESP] Ricci Junior, O. Mattos, L. C. Angulo, I. L. Bonini-Domingos, C. R. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Hemoctr Faculdade de Medicina de São José do Rio Preto (FAMERP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Viana-Baracioli, L. M. S. [UNESP] Tukamoto Junior, N. C. [UNESP] Ricci Junior, O. Mattos, L. C. Angulo, I. L. Bonini-Domingos, C. R. [UNESP] |
dc.subject.por.fl_str_mv |
Hemoglobinopathy Hemoglobin S Antioxidant capacity HFE Hemochromatosis |
topic |
Hemoglobinopathy Hemoglobin S Antioxidant capacity HFE Hemochromatosis |
description |
It is well documented that Hb S and iron affect blood cells, and trigger oxidative processes and generation of free radicals with potential for lipid peroxidation. We evaluated the frequency of polymorphisms in the HFE gene in Hb AS blood donors and how these polymorphisms influenced lipid peroxidation and antioxidant capacity. Blood samples were collected from 211 Hb AS blood donors, 119 Hb AA blood donors as a control group, and 28 sickle cell disease patients (Hb SS). The H63D allele was found at a frequency of 10.5% in the Hb AS samples, and the C282Y allele frequency was 0.7%. In the control group, the frequencies of the H63D and C282Y alleles were 13.4 and 2.1%, respectively. In the sickle-cell disease patients, the H63D and the C282Y allele frequencies were 10.7 and 3.5%, respectively. The frequencies of the C282Y and H63D polymorphisms in Hb AS blood donors are similar to those reported for the Brazilian population. Serum malondialdehyde values, indicative of lipid peroxidation, were highest in sickle cell patients, independent of the polymorphisms in the HFE gene, with significant differences, showing the influence of Hb S allele in the levels of lipid peroxidation. However, the trolox equivalent antioxidant capacity average levels, indicative of the antioxidant capacity, were reduced with significant differences, indicating that in spite of a lipid peroxidation raise, this is not followed by the increased of the antioxidant capacity, leading to oxidative stress. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 2014-05-20T15:34:42Z 2014-05-20T15:34:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/2011.December.8.4 Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 10, n. 4, p. 3446-3454, 2011. 1676-5680 http://hdl.handle.net/11449/42631 10.4238/2011.December.8.4 WOS:000300617600119 WOS000300617600119.pdf 3279428066176719 0000-0002-4603-9467 |
url |
http://dx.doi.org/10.4238/2011.December.8.4 http://hdl.handle.net/11449/42631 |
identifier_str_mv |
Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 10, n. 4, p. 3446-3454, 2011. 1676-5680 10.4238/2011.December.8.4 WOS:000300617600119 WOS000300617600119.pdf 3279428066176719 0000-0002-4603-9467 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Research 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3446-3454 application/pdf |
dc.publisher.none.fl_str_mv |
Funpec-editora |
publisher.none.fl_str_mv |
Funpec-editora |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128521384493056 |