Antimicrobial Photodynamic Therapy Mediated by Fotenticine and Methylene Blue on Planktonic Growth, Biofilms, and Burn Infections of Acinetobacter baumannii
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/antibiotics11050619 http://hdl.handle.net/11449/241851 |
Resumo: | Antimicrobial photodynamic therapy (aPDT) is considered a promising alternative strat-egy to control Acinetobacter baumannii infections. In this study, we evaluated the action of aPDT mediated by a new photosensitizer derivative from chlorin e-6 (Fotoenticine—FTC) on A. baumannii, comparing its effects with methylene blue (MB). For this, aPDT was applied on A. baumannii in planktonic growth, biofilms, and burn infections in Galleria mellonella. The absorption of FTC and MB by bacterial cells was also evaluated using microscopic and spectrophotometric analysis. The results of planktonic cultures showed that aPDT reduced the number of viable cells compared to the non-treated group for the reference and multidrug-resistant A. baumannii strains. These reduc-tions varied from 1.4 to 2 log10 CFU for FTC and from 2 log10 CFU to total inhibition for MB. In biofilms, aPDT with MB reduced 3.9 log10 CFU of A. baumannii, whereas FTC had no effect on the cell counts. In G. mellonella, only MB-mediated aPDT had antimicrobial activity on burn injuries, increasing the larvae survival by 35%. Both photosensitizers were internalized by bacterial cells, but MB showed a higher absorption compared to FTC. In conclusion, MB had greater efficacy than FTC as a photosensitizer in aPDT against A. baumannii. |
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Antimicrobial Photodynamic Therapy Mediated by Fotenticine and Methylene Blue on Planktonic Growth, Biofilms, and Burn Infections of Acinetobacter baumanniiAcinetobacter baumanniiburnschlorinfotoenticineGalleria mellonellamethylene bluephotodynamic therapyAntimicrobial photodynamic therapy (aPDT) is considered a promising alternative strat-egy to control Acinetobacter baumannii infections. In this study, we evaluated the action of aPDT mediated by a new photosensitizer derivative from chlorin e-6 (Fotoenticine—FTC) on A. baumannii, comparing its effects with methylene blue (MB). For this, aPDT was applied on A. baumannii in planktonic growth, biofilms, and burn infections in Galleria mellonella. The absorption of FTC and MB by bacterial cells was also evaluated using microscopic and spectrophotometric analysis. The results of planktonic cultures showed that aPDT reduced the number of viable cells compared to the non-treated group for the reference and multidrug-resistant A. baumannii strains. These reduc-tions varied from 1.4 to 2 log10 CFU for FTC and from 2 log10 CFU to total inhibition for MB. In biofilms, aPDT with MB reduced 3.9 log10 CFU of A. baumannii, whereas FTC had no effect on the cell counts. In G. mellonella, only MB-mediated aPDT had antimicrobial activity on burn injuries, increasing the larvae survival by 35%. Both photosensitizers were internalized by bacterial cells, but MB showed a higher absorption compared to FTC. In conclusion, MB had greater efficacy than FTC as a photosensitizer in aPDT against A. baumannii.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Institute of Science and Technology (ICT) São Paulo State University (Unesp), São PauloPhotobiology Applied to Health (Photobios) University of Vale of Paraiba/UNIVAP, São PauloInstitute of Science and Technology (ICT) São Paulo State University (Unesp), São PauloCNPq: 408369/2018-3Universidade Estadual Paulista (UNESP)University of Vale of Paraiba/UNIVAPFigueiredo-Godoi, Lívia M. A. [UNESP]Garcia, Maíra T. [UNESP]Pinto, Juliana G.Ferreira-Strixino, JulianaFaustino, Eliseu Gabriel [UNESP]Pedroso, Lara Luise Castro [UNESP]Junqueira, Juliana C. [UNESP]2023-03-02T00:30:49Z2023-03-02T00:30:49Z2022-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/antibiotics11050619Antibiotics, v. 11, n. 5, 2022.2079-6382http://hdl.handle.net/11449/24185110.3390/antibiotics110506192-s2.0-85130193112Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAntibioticsinfo:eu-repo/semantics/openAccess2023-03-02T00:30:50Zoai:repositorio.unesp.br:11449/241851Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:15:55.816868Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antimicrobial Photodynamic Therapy Mediated by Fotenticine and Methylene Blue on Planktonic Growth, Biofilms, and Burn Infections of Acinetobacter baumannii |
title |
Antimicrobial Photodynamic Therapy Mediated by Fotenticine and Methylene Blue on Planktonic Growth, Biofilms, and Burn Infections of Acinetobacter baumannii |
spellingShingle |
Antimicrobial Photodynamic Therapy Mediated by Fotenticine and Methylene Blue on Planktonic Growth, Biofilms, and Burn Infections of Acinetobacter baumannii Figueiredo-Godoi, Lívia M. A. [UNESP] Acinetobacter baumannii burns chlorin fotoenticine Galleria mellonella methylene blue photodynamic therapy |
title_short |
Antimicrobial Photodynamic Therapy Mediated by Fotenticine and Methylene Blue on Planktonic Growth, Biofilms, and Burn Infections of Acinetobacter baumannii |
title_full |
Antimicrobial Photodynamic Therapy Mediated by Fotenticine and Methylene Blue on Planktonic Growth, Biofilms, and Burn Infections of Acinetobacter baumannii |
title_fullStr |
Antimicrobial Photodynamic Therapy Mediated by Fotenticine and Methylene Blue on Planktonic Growth, Biofilms, and Burn Infections of Acinetobacter baumannii |
title_full_unstemmed |
Antimicrobial Photodynamic Therapy Mediated by Fotenticine and Methylene Blue on Planktonic Growth, Biofilms, and Burn Infections of Acinetobacter baumannii |
title_sort |
Antimicrobial Photodynamic Therapy Mediated by Fotenticine and Methylene Blue on Planktonic Growth, Biofilms, and Burn Infections of Acinetobacter baumannii |
author |
Figueiredo-Godoi, Lívia M. A. [UNESP] |
author_facet |
Figueiredo-Godoi, Lívia M. A. [UNESP] Garcia, Maíra T. [UNESP] Pinto, Juliana G. Ferreira-Strixino, Juliana Faustino, Eliseu Gabriel [UNESP] Pedroso, Lara Luise Castro [UNESP] Junqueira, Juliana C. [UNESP] |
author_role |
author |
author2 |
Garcia, Maíra T. [UNESP] Pinto, Juliana G. Ferreira-Strixino, Juliana Faustino, Eliseu Gabriel [UNESP] Pedroso, Lara Luise Castro [UNESP] Junqueira, Juliana C. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Vale of Paraiba/UNIVAP |
dc.contributor.author.fl_str_mv |
Figueiredo-Godoi, Lívia M. A. [UNESP] Garcia, Maíra T. [UNESP] Pinto, Juliana G. Ferreira-Strixino, Juliana Faustino, Eliseu Gabriel [UNESP] Pedroso, Lara Luise Castro [UNESP] Junqueira, Juliana C. [UNESP] |
dc.subject.por.fl_str_mv |
Acinetobacter baumannii burns chlorin fotoenticine Galleria mellonella methylene blue photodynamic therapy |
topic |
Acinetobacter baumannii burns chlorin fotoenticine Galleria mellonella methylene blue photodynamic therapy |
description |
Antimicrobial photodynamic therapy (aPDT) is considered a promising alternative strat-egy to control Acinetobacter baumannii infections. In this study, we evaluated the action of aPDT mediated by a new photosensitizer derivative from chlorin e-6 (Fotoenticine—FTC) on A. baumannii, comparing its effects with methylene blue (MB). For this, aPDT was applied on A. baumannii in planktonic growth, biofilms, and burn infections in Galleria mellonella. The absorption of FTC and MB by bacterial cells was also evaluated using microscopic and spectrophotometric analysis. The results of planktonic cultures showed that aPDT reduced the number of viable cells compared to the non-treated group for the reference and multidrug-resistant A. baumannii strains. These reduc-tions varied from 1.4 to 2 log10 CFU for FTC and from 2 log10 CFU to total inhibition for MB. In biofilms, aPDT with MB reduced 3.9 log10 CFU of A. baumannii, whereas FTC had no effect on the cell counts. In G. mellonella, only MB-mediated aPDT had antimicrobial activity on burn injuries, increasing the larvae survival by 35%. Both photosensitizers were internalized by bacterial cells, but MB showed a higher absorption compared to FTC. In conclusion, MB had greater efficacy than FTC as a photosensitizer in aPDT against A. baumannii. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01 2023-03-02T00:30:49Z 2023-03-02T00:30:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/antibiotics11050619 Antibiotics, v. 11, n. 5, 2022. 2079-6382 http://hdl.handle.net/11449/241851 10.3390/antibiotics11050619 2-s2.0-85130193112 |
url |
http://dx.doi.org/10.3390/antibiotics11050619 http://hdl.handle.net/11449/241851 |
identifier_str_mv |
Antibiotics, v. 11, n. 5, 2022. 2079-6382 10.3390/antibiotics11050619 2-s2.0-85130193112 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Antibiotics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129411662217216 |