Estudo de novas alternativas biológicas na eliminação de microrganismos envolvidos nas infecções endodônticas

• Autor(a) principal: Lourenço, Lucas Faria Morini [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/155207; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-05-08/000880892.pdf
• topic: Biofilmes; Silimarina; Enterococcus faecalis; Biofilms

Endodontic infections occur because of the microorganisms invasion of the root canal system and can reach the periradicular tissues. The first step in endodontic therapy consisted on the elimination of microorganisms and prevention of reinfection of root canals. Herbal substances have been incorporated in various dental products, due to its anti-inflammatory, antibacterial and antifungal activities, besides no promote antibiotic resistance and be biocompatible at therapeutic concentrations, creating new biological perspectives for dental treatment. The objective of this study was to evaluate the antimicrobial action of silymarin on planktonic culture and on biofilms of Enterococcus faecalis (ATCC 51299). Planctonic assays were performed to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silymarin. Biofilms of E. faecalis were exposed to 5X and 10X MIC silymarin (SIL) and the control chlorhexidine digluconate (CHX). After exposure for 24h, the cultures were plated on Mueller-Hinton agar and incubated for 24 hours to count the colony forming units (CFU) per ml. The data were statistically analyzed, considering p <0.05. The results showed that the SIL at 1.25mg/mL was considered CIM because reduced 51.62% of E. faecalis growth, however, only SIL at 5mg/mL was sufficient to eliminate 100% of E. faecalis and considered CBM. In biofilm tests, SIL 10x MIC was the agent who showed the best effect against biofilms of E. faecalis, reducing five logarithmic houses of E. faecalis growth. SIL 5x and 10x CHX showed similar results, reducing two logarithmic houses of E. faecalis growth. CHX 5X did not differ from controls. Concludes that silymarin has action against E. faecalis in planktonic and biofilm conditions, showing a promising biological agent for the elimination of microorganisms involved in endodontic infections