Probiotics study with Streptococcus salivarius and its ability to produce bacteriocins and adherence to KB cells
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1807-2577.02919 http://hdl.handle.net/11449/183793 |
Resumo: | AbstractIntroduction Streptococcus salivarius is a dominant oral species and the best suitable candidate for probiotic of the oral cavity. Since Streptococcus salivarius is able to produce bacteriocins against Streptococcus pyogenes interest has been focused on the use of it as a probiotic to avoid sore throats by Streptococcus pyogenes.Objective This study is for selecting Streptococcus salivarius strains for potential use as probiotics for the oral mucosa, that is, production of bacteriocin against Streptococcus pyogenes and the ability to bind to KB cells.Material and method Tongue material from 45 students was collected and seeded on Mitis Salivarius Agar plaques. The strains were tested by the production of bacteriocin-like substances (BLIS) against S. pyogenes, biochemically and PCR for identification of S. salivarius. The best strains were tested for adherence to KB cells. Briefly, S. salivarius strains were cultured in broth, washed and suspended at 108cells/ml. KB cells were inoculated into plaques, washed and incubated with the bacteria, for adhesion. These were washed for lysis of the KB cells and release bacteria for determination of CFU.Result The bacteriocin test showed that 133 strains presented inhibition of S. pyogenes. The samples tested for adhesion to KB cells, presented different profiles and only three strains presenting high adhesion capacity.Conclusion The selection of strains of Streptococcus salivarius with high inhibitory activity against Streptococcus pyogenes, as well as adherence to KB cells leads us to the next future step, that is, to use the best strains for in vivo colonization tests |
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Probiotics study with Streptococcus salivarius and its ability to produce bacteriocins and adherence to KB cellsEstudo de probióticos Streptococcus salivarius e sua habilidade de produzir bacteriocinas e aderência à células KBProbioticsStreptococcus salivariusStreptococcus pyogenesantagonismbacteriocinadhesion KB cellProbióticosStreptococcus salivariusStreptococcus pyogenesantagonismobacteriocinaaderência a células KBAbstractIntroduction Streptococcus salivarius is a dominant oral species and the best suitable candidate for probiotic of the oral cavity. Since Streptococcus salivarius is able to produce bacteriocins against Streptococcus pyogenes interest has been focused on the use of it as a probiotic to avoid sore throats by Streptococcus pyogenes.Objective This study is for selecting Streptococcus salivarius strains for potential use as probiotics for the oral mucosa, that is, production of bacteriocin against Streptococcus pyogenes and the ability to bind to KB cells.Material and method Tongue material from 45 students was collected and seeded on Mitis Salivarius Agar plaques. The strains were tested by the production of bacteriocin-like substances (BLIS) against S. pyogenes, biochemically and PCR for identification of S. salivarius. The best strains were tested for adherence to KB cells. Briefly, S. salivarius strains were cultured in broth, washed and suspended at 108cells/ml. KB cells were inoculated into plaques, washed and incubated with the bacteria, for adhesion. These were washed for lysis of the KB cells and release bacteria for determination of CFU.Result The bacteriocin test showed that 133 strains presented inhibition of S. pyogenes. The samples tested for adhesion to KB cells, presented different profiles and only three strains presenting high adhesion capacity.Conclusion The selection of strains of Streptococcus salivarius with high inhibitory activity against Streptococcus pyogenes, as well as adherence to KB cells leads us to the next future step, that is, to use the best strains for in vivo colonization testsResumoIntrodução Streptococcus salivarius é uma espécie dominante na cavidade bucal e tem sido indicada como um ótimo candidato para uso como probiótico. Visto que a espécie Streptococcus salivarius é capaz de produzir bacteriocinas contra Streptococcus pyogenes, desenvolveu-se interesse no uso desse microrganismo como probiótico, para evitar amigdalites causadas por Streptococcus pyogenes.Objetivo A pesquisa em questão tem o objetivo de selecionar cepas de Streptococcus salivarius para seu uso potencial como probióticos na cavidade bucal, ou seja, produção de bacteriocinas contra Streptococcus pyogenes e habilidade de aderência à células KB.Material e método Coletou-se material de língua de 45 estudantes e semeou-se em placas de ágar Mitis Salivarius. As amostras foram testadas para verificar a produção de substâncias semelhantes à bacteriocina (BLIS) contra S. pyogenes, bioquimicamente e através de PCR para identificação de S. salivarius. As melhores cepas foram testadas quanto aderência à células KB. Resumidamente, as cepas de S. salivarius foram cultivadas em caldo, lavadas e suspensas à correspondência de 108 cels/ml. As células KB foram inoculadas em placas, lavadas e incubadas com as bactérias, para adesão. Estas foram lavadas para lise das células KB e liberação das bactérias para determinação de UFC.Resultado O teste de bacteriocina, mostrou que 133 cepas apresentaram atividade inibitória contra Streptococcus pyogenes. As cepas testadas para aderência à células KB, apresentaram diferentes perfis e somente três com alta capacidade de adesão. Conclusão: A seleção de cepas de Streptococcus salivarius com alta atividade inibitória contra Streptococcus pyogenes, bem como aderência a células KB, pode nos levar ao próximo passo, ou seja, o uso das melhores cepas para o estudo de colonização in vivo.Universidade Estadual Paulista Instituto de Ciência e TecnologiaCHT Quimipel Brazil Química Ltda Técnica de LaboratórioDuas Rodas Centro de InovaçãoUniversidade Estadual Paulista Instituto de Ciência e TecnologiaUniversidade Estadual Paulista Júlio de Mesquita FilhoUniversidade Estadual Paulista (Unesp)CHT Quimipel Brazil Química Ltda Técnica de LaboratórioDuas Rodas Centro de InovaçãoFantinato, VeraCamargo, Heloísa RamalhoSousa, Ana Lúcia Orlandinni Pilleggi De2019-10-03T17:31:40Z2019-10-03T17:31:40Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1807-2577.02919Revista de Odontologia da UNESP. Universidade Estadual Paulista Júlio de Mesquita Filho, v. 48, p. -, 2019.1807-2577http://hdl.handle.net/11449/18379310.1590/1807-2577.02919S1807-25772019000100417S1807-25772019000100417.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista de Odontologia da UNESPinfo:eu-repo/semantics/openAccess2023-10-15T06:03:16Zoai:repositorio.unesp.br:11449/183793Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-15T06:03:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Probiotics study with Streptococcus salivarius and its ability to produce bacteriocins and adherence to KB cells Estudo de probióticos Streptococcus salivarius e sua habilidade de produzir bacteriocinas e aderência à células KB |
title |
Probiotics study with Streptococcus salivarius and its ability to produce bacteriocins and adherence to KB cells |
spellingShingle |
Probiotics study with Streptococcus salivarius and its ability to produce bacteriocins and adherence to KB cells Fantinato, Vera Probiotics Streptococcus salivarius Streptococcus pyogenes antagonism bacteriocin adhesion KB cell Probióticos Streptococcus salivarius Streptococcus pyogenes antagonismo bacteriocina aderência a células KB |
title_short |
Probiotics study with Streptococcus salivarius and its ability to produce bacteriocins and adherence to KB cells |
title_full |
Probiotics study with Streptococcus salivarius and its ability to produce bacteriocins and adherence to KB cells |
title_fullStr |
Probiotics study with Streptococcus salivarius and its ability to produce bacteriocins and adherence to KB cells |
title_full_unstemmed |
Probiotics study with Streptococcus salivarius and its ability to produce bacteriocins and adherence to KB cells |
title_sort |
Probiotics study with Streptococcus salivarius and its ability to produce bacteriocins and adherence to KB cells |
author |
Fantinato, Vera |
author_facet |
Fantinato, Vera Camargo, Heloísa Ramalho Sousa, Ana Lúcia Orlandinni Pilleggi De |
author_role |
author |
author2 |
Camargo, Heloísa Ramalho Sousa, Ana Lúcia Orlandinni Pilleggi De |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) CHT Quimipel Brazil Química Ltda Técnica de Laboratório Duas Rodas Centro de Inovação |
dc.contributor.author.fl_str_mv |
Fantinato, Vera Camargo, Heloísa Ramalho Sousa, Ana Lúcia Orlandinni Pilleggi De |
dc.subject.por.fl_str_mv |
Probiotics Streptococcus salivarius Streptococcus pyogenes antagonism bacteriocin adhesion KB cell Probióticos Streptococcus salivarius Streptococcus pyogenes antagonismo bacteriocina aderência a células KB |
topic |
Probiotics Streptococcus salivarius Streptococcus pyogenes antagonism bacteriocin adhesion KB cell Probióticos Streptococcus salivarius Streptococcus pyogenes antagonismo bacteriocina aderência a células KB |
description |
AbstractIntroduction Streptococcus salivarius is a dominant oral species and the best suitable candidate for probiotic of the oral cavity. Since Streptococcus salivarius is able to produce bacteriocins against Streptococcus pyogenes interest has been focused on the use of it as a probiotic to avoid sore throats by Streptococcus pyogenes.Objective This study is for selecting Streptococcus salivarius strains for potential use as probiotics for the oral mucosa, that is, production of bacteriocin against Streptococcus pyogenes and the ability to bind to KB cells.Material and method Tongue material from 45 students was collected and seeded on Mitis Salivarius Agar plaques. The strains were tested by the production of bacteriocin-like substances (BLIS) against S. pyogenes, biochemically and PCR for identification of S. salivarius. The best strains were tested for adherence to KB cells. Briefly, S. salivarius strains were cultured in broth, washed and suspended at 108cells/ml. KB cells were inoculated into plaques, washed and incubated with the bacteria, for adhesion. These were washed for lysis of the KB cells and release bacteria for determination of CFU.Result The bacteriocin test showed that 133 strains presented inhibition of S. pyogenes. The samples tested for adhesion to KB cells, presented different profiles and only three strains presenting high adhesion capacity.Conclusion The selection of strains of Streptococcus salivarius with high inhibitory activity against Streptococcus pyogenes, as well as adherence to KB cells leads us to the next future step, that is, to use the best strains for in vivo colonization tests |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-03T17:31:40Z 2019-10-03T17:31:40Z 2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1807-2577.02919 Revista de Odontologia da UNESP. Universidade Estadual Paulista Júlio de Mesquita Filho, v. 48, p. -, 2019. 1807-2577 http://hdl.handle.net/11449/183793 10.1590/1807-2577.02919 S1807-25772019000100417 S1807-25772019000100417.pdf |
url |
http://dx.doi.org/10.1590/1807-2577.02919 http://hdl.handle.net/11449/183793 |
identifier_str_mv |
Revista de Odontologia da UNESP. Universidade Estadual Paulista Júlio de Mesquita Filho, v. 48, p. -, 2019. 1807-2577 10.1590/1807-2577.02919 S1807-25772019000100417 S1807-25772019000100417.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista de Odontologia da UNESP |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista Júlio de Mesquita Filho |
publisher.none.fl_str_mv |
Universidade Estadual Paulista Júlio de Mesquita Filho |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964565258108928 |