Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)

Detalhes bibliográficos
Autor(a) principal: Novelli, Valdenice M. [UNESP]
Data de Publicação: 2006
Outros Autores: Cristofani, Mariangela, Souza, Alessandra A., Machado, Marcos A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1415-47572006000100018
http://hdl.handle.net/11449/30481
Resumo: The aims of this study were to develop simple sequence repeat markers (SSRs or microsatellite markers) in citrus and to evaluate the efficiency of these markers for characterization of sweet orange. We developed SSRs from a genomic library of 'Pêra IAC' sweet orange enriched for AG/TC, GT/CA, TCA/AGT and AAC/TTG sequence repeats. We selected 279 sequences from which 171 primer pairs were designed of which 113 with the best banding patterns were selected. Characterization of sweet orange microsatellite loci revealed that AG/TC was the most abundant (69%) microsatellite class isolated, followed by GT/CA (15.9%), TCA/AGT (8%) and AAC/TTG (6.2%). The number of alleles ranged from 1 to 4, with a mean of 2 alleles per locus. Four microsatellite loci developed in this study were found to be useful for sweet orange DNA typing. The data obtained from microsatellites loci considered polymorphic will be useful as tools in the selection of zygotic and nucellar plants, identification of seedlings etc. for the cultivars Pêra IAC, Lanceta, Pêra GS 2000, Lamb Summer, Lima, Lima Tardia, Lima Verde, Mimo do Céu, Valência Folha Murcha, Valência Folha Concha, Natal Murcha, Sangüínea and Baía Gigante.
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spelling Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)citrus speciesgermplasm bankpolymorphismmolecular markersThe aims of this study were to develop simple sequence repeat markers (SSRs or microsatellite markers) in citrus and to evaluate the efficiency of these markers for characterization of sweet orange. We developed SSRs from a genomic library of 'Pêra IAC' sweet orange enriched for AG/TC, GT/CA, TCA/AGT and AAC/TTG sequence repeats. We selected 279 sequences from which 171 primer pairs were designed of which 113 with the best banding patterns were selected. Characterization of sweet orange microsatellite loci revealed that AG/TC was the most abundant (69%) microsatellite class isolated, followed by GT/CA (15.9%), TCA/AGT (8%) and AAC/TTG (6.2%). The number of alleles ranged from 1 to 4, with a mean of 2 alleles per locus. Four microsatellite loci developed in this study were found to be useful for sweet orange DNA typing. The data obtained from microsatellites loci considered polymorphic will be useful as tools in the selection of zygotic and nucellar plants, identification of seedlings etc. for the cultivars Pêra IAC, Lanceta, Pêra GS 2000, Lamb Summer, Lima, Lima Tardia, Lima Verde, Mimo do Céu, Valência Folha Murcha, Valência Folha Concha, Natal Murcha, Sangüínea and Baía Gigante.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista Júlio de Mesquita Filho Instituto de BiociênciasInstituto Agronômico de Campinas Centro APTA Citros Sylvio MoreiraUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de BiociênciasSociedade Brasileira de GenéticaUniversidade Estadual Paulista (Unesp)Agência Paulista de Tecnologia dos Agronegócios (APTA)Novelli, Valdenice M. [UNESP]Cristofani, MariangelaSouza, Alessandra A.Machado, Marcos A.2014-05-20T15:17:28Z2014-05-20T15:17:28Z2006-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article90-96application/pdfhttp://dx.doi.org/10.1590/S1415-47572006000100018Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 29, n. 1, p. 90-96, 2006.1415-4757http://hdl.handle.net/11449/3048110.1590/S1415-47572006000100018S1415-47572006000100018S1415-47572006000100018.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-10-16T06:06:41Zoai:repositorio.unesp.br:11449/30481Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-16T06:06:41Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)
title Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)
spellingShingle Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)
Novelli, Valdenice M. [UNESP]
citrus species
germplasm bank
polymorphism
molecular markers
title_short Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)
title_full Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)
title_fullStr Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)
title_full_unstemmed Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)
title_sort Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)
author Novelli, Valdenice M. [UNESP]
author_facet Novelli, Valdenice M. [UNESP]
Cristofani, Mariangela
Souza, Alessandra A.
Machado, Marcos A.
author_role author
author2 Cristofani, Mariangela
Souza, Alessandra A.
Machado, Marcos A.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Agência Paulista de Tecnologia dos Agronegócios (APTA)
dc.contributor.author.fl_str_mv Novelli, Valdenice M. [UNESP]
Cristofani, Mariangela
Souza, Alessandra A.
Machado, Marcos A.
dc.subject.por.fl_str_mv citrus species
germplasm bank
polymorphism
molecular markers
topic citrus species
germplasm bank
polymorphism
molecular markers
description The aims of this study were to develop simple sequence repeat markers (SSRs or microsatellite markers) in citrus and to evaluate the efficiency of these markers for characterization of sweet orange. We developed SSRs from a genomic library of 'Pêra IAC' sweet orange enriched for AG/TC, GT/CA, TCA/AGT and AAC/TTG sequence repeats. We selected 279 sequences from which 171 primer pairs were designed of which 113 with the best banding patterns were selected. Characterization of sweet orange microsatellite loci revealed that AG/TC was the most abundant (69%) microsatellite class isolated, followed by GT/CA (15.9%), TCA/AGT (8%) and AAC/TTG (6.2%). The number of alleles ranged from 1 to 4, with a mean of 2 alleles per locus. Four microsatellite loci developed in this study were found to be useful for sweet orange DNA typing. The data obtained from microsatellites loci considered polymorphic will be useful as tools in the selection of zygotic and nucellar plants, identification of seedlings etc. for the cultivars Pêra IAC, Lanceta, Pêra GS 2000, Lamb Summer, Lima, Lima Tardia, Lima Verde, Mimo do Céu, Valência Folha Murcha, Valência Folha Concha, Natal Murcha, Sangüínea and Baía Gigante.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-01
2014-05-20T15:17:28Z
2014-05-20T15:17:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572006000100018
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 29, n. 1, p. 90-96, 2006.
1415-4757
http://hdl.handle.net/11449/30481
10.1590/S1415-47572006000100018
S1415-47572006000100018
S1415-47572006000100018.pdf
url http://dx.doi.org/10.1590/S1415-47572006000100018
http://hdl.handle.net/11449/30481
identifier_str_mv Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 29, n. 1, p. 90-96, 2006.
1415-4757
10.1590/S1415-47572006000100018
S1415-47572006000100018
S1415-47572006000100018.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Biology
1.493
0,638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 90-96
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1803046020559405056

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