Ocorrência e caracterização molecular de Cryptosporidium em cordeiros
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0102-09352009000400033 http://hdl.handle.net/11449/14867 |
Resumo: | The occurrence of Cryptosporidium in fecal samples of lambs was determined in order to test correlations among the type of animal raising, feces consistence, index of oocysts elimination, animal age, and molecular characterization of species and genotypes involved in this parasitism. A total of 460 fecal samples were collected from lambs during the first 30 days of life, in various screeds of 21 farms. All samples were analyzed using malachite green negative coloration stain under microscopy. For molecular characterization of Cryptosporidium, on positive samples under microscopy, a nested PCR protocol was used for amplification of fragmentts from 18S rRNA gene or actin gene. It was found 6.7% of animals expeling oocysts of Cryptosporidium in feces of eight farms, without statistics relation among the analyzed indexes. The species and genotype involved were C. parvum type A 18S rRNA, C. parvum type B 18S rRNA and cervid genotype, which present a zoonotic potential of disease. |
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Ocorrência e caracterização molecular de Cryptosporidium em cordeirosOccurrence and molecular characterization of Cryptosporidium in lambsThe occurrence of Cryptosporidium in fecal samples of lambs was determined in order to test correlations among the type of animal raising, feces consistence, index of oocysts elimination, animal age, and molecular characterization of species and genotypes involved in this parasitism. A total of 460 fecal samples were collected from lambs during the first 30 days of life, in various screeds of 21 farms. All samples were analyzed using malachite green negative coloration stain under microscopy. For molecular characterization of Cryptosporidium, on positive samples under microscopy, a nested PCR protocol was used for amplification of fragmentts from 18S rRNA gene or actin gene. It was found 6.7% of animals expeling oocysts of Cryptosporidium in feces of eight farms, without statistics relation among the analyzed indexes. The species and genotype involved were C. parvum type A 18S rRNA, C. parvum type B 18S rRNA and cervid genotype, which present a zoonotic potential of disease.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP, FO, BR-16050680 Aracatuba, SP, BrazilUNESP, FO, BR-16050680 Aracatuba, SP, BrazilUniversidade Federal de Minas Gerais (UFMG), Escola de VeterináriaUniversidade Estadual Paulista (Unesp)Féres, F. C. [UNESP]Lombardi, A. L. [UNESP]Carvalho, M. P. P. [UNESP]Mendes, Luiz Claudio Nogueira [UNESP]Peiró, Juliana Regina [UNESP]Cadioli, Fabiano Antonio [UNESP]Meireles, Marcelo Vasconcelos [UNESP]Perri, Silvia Helena Venturoli [UNESP]Feitosa, Francisco Leydson Formiga [UNESP]2013-09-30T19:50:03Z2014-05-20T13:42:45Z2013-09-30T19:50:03Z2014-05-20T13:42:45Z2009-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1002-1005application/pdfhttp://dx.doi.org/10.1590/S0102-09352009000400033Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 61, n. 4, p. 1002-1005, 2009.0102-0935http://hdl.handle.net/11449/1486710.1590/S0102-09352009000400033S0102-09352009000400033WOS:000272404400033S0102-09352009000400033.pdf2232625970712015297612987455469838842890760710830903513897615274081083759820263463205878717780380000-0003-0063-5172SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporArquivo Brasileiro de Medicina Veterinária e Zootecnia0.2860,248info:eu-repo/semantics/openAccess2024-01-23T07:09:50Zoai:repositorio.unesp.br:11449/14867Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-23T07:09:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ocorrência e caracterização molecular de Cryptosporidium em cordeiros Occurrence and molecular characterization of Cryptosporidium in lambs |
title |
Ocorrência e caracterização molecular de Cryptosporidium em cordeiros |
spellingShingle |
Ocorrência e caracterização molecular de Cryptosporidium em cordeiros Féres, F. C. [UNESP] |
title_short |
Ocorrência e caracterização molecular de Cryptosporidium em cordeiros |
title_full |
Ocorrência e caracterização molecular de Cryptosporidium em cordeiros |
title_fullStr |
Ocorrência e caracterização molecular de Cryptosporidium em cordeiros |
title_full_unstemmed |
Ocorrência e caracterização molecular de Cryptosporidium em cordeiros |
title_sort |
Ocorrência e caracterização molecular de Cryptosporidium em cordeiros |
author |
Féres, F. C. [UNESP] |
author_facet |
Féres, F. C. [UNESP] Lombardi, A. L. [UNESP] Carvalho, M. P. P. [UNESP] Mendes, Luiz Claudio Nogueira [UNESP] Peiró, Juliana Regina [UNESP] Cadioli, Fabiano Antonio [UNESP] Meireles, Marcelo Vasconcelos [UNESP] Perri, Silvia Helena Venturoli [UNESP] Feitosa, Francisco Leydson Formiga [UNESP] |
author_role |
author |
author2 |
Lombardi, A. L. [UNESP] Carvalho, M. P. P. [UNESP] Mendes, Luiz Claudio Nogueira [UNESP] Peiró, Juliana Regina [UNESP] Cadioli, Fabiano Antonio [UNESP] Meireles, Marcelo Vasconcelos [UNESP] Perri, Silvia Helena Venturoli [UNESP] Feitosa, Francisco Leydson Formiga [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Féres, F. C. [UNESP] Lombardi, A. L. [UNESP] Carvalho, M. P. P. [UNESP] Mendes, Luiz Claudio Nogueira [UNESP] Peiró, Juliana Regina [UNESP] Cadioli, Fabiano Antonio [UNESP] Meireles, Marcelo Vasconcelos [UNESP] Perri, Silvia Helena Venturoli [UNESP] Feitosa, Francisco Leydson Formiga [UNESP] |
description |
The occurrence of Cryptosporidium in fecal samples of lambs was determined in order to test correlations among the type of animal raising, feces consistence, index of oocysts elimination, animal age, and molecular characterization of species and genotypes involved in this parasitism. A total of 460 fecal samples were collected from lambs during the first 30 days of life, in various screeds of 21 farms. All samples were analyzed using malachite green negative coloration stain under microscopy. For molecular characterization of Cryptosporidium, on positive samples under microscopy, a nested PCR protocol was used for amplification of fragmentts from 18S rRNA gene or actin gene. It was found 6.7% of animals expeling oocysts of Cryptosporidium in feces of eight farms, without statistics relation among the analyzed indexes. The species and genotype involved were C. parvum type A 18S rRNA, C. parvum type B 18S rRNA and cervid genotype, which present a zoonotic potential of disease. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-08-01 2013-09-30T19:50:03Z 2013-09-30T19:50:03Z 2014-05-20T13:42:45Z 2014-05-20T13:42:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-09352009000400033 Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 61, n. 4, p. 1002-1005, 2009. 0102-0935 http://hdl.handle.net/11449/14867 10.1590/S0102-09352009000400033 S0102-09352009000400033 WOS:000272404400033 S0102-09352009000400033.pdf 2232625970712015 2976129874554698 3884289076071083 0903513897615274 0810837598202634 6320587871778038 0000-0003-0063-5172 |
url |
http://dx.doi.org/10.1590/S0102-09352009000400033 http://hdl.handle.net/11449/14867 |
identifier_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 61, n. 4, p. 1002-1005, 2009. 0102-0935 10.1590/S0102-09352009000400033 S0102-09352009000400033 WOS:000272404400033 S0102-09352009000400033.pdf 2232625970712015 2976129874554698 3884289076071083 0903513897615274 0810837598202634 6320587871778038 0000-0003-0063-5172 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia 0.286 0,248 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1002-1005 application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965705698803712 |