Polymorphisms in TLR4 Gene Associated With Somatic Cell Score in Water Buffaloes (Bubalus bubalis)
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fvets.2020.568249 http://hdl.handle.net/11449/208152 |
Resumo: | Considering the importance of the diseases affecting the productive performance of animals in the dairy industry worldwide, it is necessary to implement tools that help to control and limit the occurrence of such diseases. As the increased somatic cell counts (SCC) are a direct expression of the inflammatory process, they are candidates to become the usual parameter for assessing udder health regarding milk quality and for monitoring mastitis incidences. Toll-Like Receptors are membrane proteins that play a key role in immunity, recognizing pathogens and, subsequently, activating immune responses. The present study was conducted to identify single nucleotide polymorphisms in the TLR4 gene of buffaloes and to analyze its associations with somatic cell counts. DNA samples of 120 Murrah buffaloes were used. The whole coding region of the TLR4 gene was amplified by polymerase chain reaction reactions and sequenced for polymorphism scanning. A total of 13 polymorphisms were identified for the sequenced regions of the TLR4, most of which are in the coding region. The association with the somatic cell score was highly significant (p < 0.001) for all identified polymorphisms of TLR4 gene (g.54621T>A, g.54429G>T, g.54407T>A, g.46616C>A, g.46613T>G, g.46612A>G, g.46611C>A, g.46609T>G, g.46541C>G, g.46526C>A, g.46516T>C, g.46376C>T, g.46372T>C). Therefore, it is suggested that the markers of the TLR4 gene can be used as molecular markers for mastitis resistance in buffaloes, due to their association with somatic cell counts. |
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Polymorphisms in TLR4 Gene Associated With Somatic Cell Score in Water Buffaloes (Bubalus bubalis)mastitismilk yieldmolecular marker analysisSNPstop codonudder healthConsidering the importance of the diseases affecting the productive performance of animals in the dairy industry worldwide, it is necessary to implement tools that help to control and limit the occurrence of such diseases. As the increased somatic cell counts (SCC) are a direct expression of the inflammatory process, they are candidates to become the usual parameter for assessing udder health regarding milk quality and for monitoring mastitis incidences. Toll-Like Receptors are membrane proteins that play a key role in immunity, recognizing pathogens and, subsequently, activating immune responses. The present study was conducted to identify single nucleotide polymorphisms in the TLR4 gene of buffaloes and to analyze its associations with somatic cell counts. DNA samples of 120 Murrah buffaloes were used. The whole coding region of the TLR4 gene was amplified by polymerase chain reaction reactions and sequenced for polymorphism scanning. A total of 13 polymorphisms were identified for the sequenced regions of the TLR4, most of which are in the coding region. The association with the somatic cell score was highly significant (p < 0.001) for all identified polymorphisms of TLR4 gene (g.54621T>A, g.54429G>T, g.54407T>A, g.46616C>A, g.46613T>G, g.46612A>G, g.46611C>A, g.46609T>G, g.46541C>G, g.46526C>A, g.46516T>C, g.46376C>T, g.46372T>C). Therefore, it is suggested that the markers of the TLR4 gene can be used as molecular markers for mastitis resistance in buffaloes, due to their association with somatic cell counts.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Animal Science School of Agricultural and Veterinarian Science São Paulo State University (UNESP)Department of Animal Biosciences Centre for Genetic Improvement of Livestock University of GuelphFacultad de Ciências Agrárias Universidad Francisco de Paula SantanderDepartment of Animal and Avian Sciences University of MarylandEscola de Medicina Veterinária e Zootecnia Universidade Federal da BahiaDepartment of Animal Science School of Agricultural and Veterinarian Science São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)University of GuelphUniversidad Francisco de Paula SantanderUniversity of MarylandUniversidade Federal da Bahia (UFBA)Roldan-Montes, Valentina [UNESP]Cardoso, Diercles Francisco [UNESP]Hurtado-Lugo, Naudin Alejandrodo Nascimento, André Vieira [UNESP]Santos, Daniel Jordan de Abreu [UNESP]Scalez, Daiane Cristina Becker [UNESP]de Freitas, Ana Cláudia [UNESP]Herrera, Ana Cristina [UNESP]Albuquerque, Lucia Galvão [UNESP]de Camargo, Gregório Miguel FerreiraTonhati, Humberto [UNESP]2021-06-25T11:07:16Z2021-06-25T11:07:16Z2020-11-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fvets.2020.568249Frontiers in Veterinary Science, v. 7.2297-1769http://hdl.handle.net/11449/20815210.3389/fvets.2020.5682492-s2.0-85096223728Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Veterinary Scienceinfo:eu-repo/semantics/openAccess2024-06-07T18:43:47Zoai:repositorio.unesp.br:11449/208152Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:10:50.759289Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Polymorphisms in TLR4 Gene Associated With Somatic Cell Score in Water Buffaloes (Bubalus bubalis) |
title |
Polymorphisms in TLR4 Gene Associated With Somatic Cell Score in Water Buffaloes (Bubalus bubalis) |
spellingShingle |
Polymorphisms in TLR4 Gene Associated With Somatic Cell Score in Water Buffaloes (Bubalus bubalis) Roldan-Montes, Valentina [UNESP] mastitis milk yield molecular marker analysis SNP stop codon udder health |
title_short |
Polymorphisms in TLR4 Gene Associated With Somatic Cell Score in Water Buffaloes (Bubalus bubalis) |
title_full |
Polymorphisms in TLR4 Gene Associated With Somatic Cell Score in Water Buffaloes (Bubalus bubalis) |
title_fullStr |
Polymorphisms in TLR4 Gene Associated With Somatic Cell Score in Water Buffaloes (Bubalus bubalis) |
title_full_unstemmed |
Polymorphisms in TLR4 Gene Associated With Somatic Cell Score in Water Buffaloes (Bubalus bubalis) |
title_sort |
Polymorphisms in TLR4 Gene Associated With Somatic Cell Score in Water Buffaloes (Bubalus bubalis) |
author |
Roldan-Montes, Valentina [UNESP] |
author_facet |
Roldan-Montes, Valentina [UNESP] Cardoso, Diercles Francisco [UNESP] Hurtado-Lugo, Naudin Alejandro do Nascimento, André Vieira [UNESP] Santos, Daniel Jordan de Abreu [UNESP] Scalez, Daiane Cristina Becker [UNESP] de Freitas, Ana Cláudia [UNESP] Herrera, Ana Cristina [UNESP] Albuquerque, Lucia Galvão [UNESP] de Camargo, Gregório Miguel Ferreira Tonhati, Humberto [UNESP] |
author_role |
author |
author2 |
Cardoso, Diercles Francisco [UNESP] Hurtado-Lugo, Naudin Alejandro do Nascimento, André Vieira [UNESP] Santos, Daniel Jordan de Abreu [UNESP] Scalez, Daiane Cristina Becker [UNESP] de Freitas, Ana Cláudia [UNESP] Herrera, Ana Cristina [UNESP] Albuquerque, Lucia Galvão [UNESP] de Camargo, Gregório Miguel Ferreira Tonhati, Humberto [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Guelph Universidad Francisco de Paula Santander University of Maryland Universidade Federal da Bahia (UFBA) |
dc.contributor.author.fl_str_mv |
Roldan-Montes, Valentina [UNESP] Cardoso, Diercles Francisco [UNESP] Hurtado-Lugo, Naudin Alejandro do Nascimento, André Vieira [UNESP] Santos, Daniel Jordan de Abreu [UNESP] Scalez, Daiane Cristina Becker [UNESP] de Freitas, Ana Cláudia [UNESP] Herrera, Ana Cristina [UNESP] Albuquerque, Lucia Galvão [UNESP] de Camargo, Gregório Miguel Ferreira Tonhati, Humberto [UNESP] |
dc.subject.por.fl_str_mv |
mastitis milk yield molecular marker analysis SNP stop codon udder health |
topic |
mastitis milk yield molecular marker analysis SNP stop codon udder health |
description |
Considering the importance of the diseases affecting the productive performance of animals in the dairy industry worldwide, it is necessary to implement tools that help to control and limit the occurrence of such diseases. As the increased somatic cell counts (SCC) are a direct expression of the inflammatory process, they are candidates to become the usual parameter for assessing udder health regarding milk quality and for monitoring mastitis incidences. Toll-Like Receptors are membrane proteins that play a key role in immunity, recognizing pathogens and, subsequently, activating immune responses. The present study was conducted to identify single nucleotide polymorphisms in the TLR4 gene of buffaloes and to analyze its associations with somatic cell counts. DNA samples of 120 Murrah buffaloes were used. The whole coding region of the TLR4 gene was amplified by polymerase chain reaction reactions and sequenced for polymorphism scanning. A total of 13 polymorphisms were identified for the sequenced regions of the TLR4, most of which are in the coding region. The association with the somatic cell score was highly significant (p < 0.001) for all identified polymorphisms of TLR4 gene (g.54621T>A, g.54429G>T, g.54407T>A, g.46616C>A, g.46613T>G, g.46612A>G, g.46611C>A, g.46609T>G, g.46541C>G, g.46526C>A, g.46516T>C, g.46376C>T, g.46372T>C). Therefore, it is suggested that the markers of the TLR4 gene can be used as molecular markers for mastitis resistance in buffaloes, due to their association with somatic cell counts. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-05 2021-06-25T11:07:16Z 2021-06-25T11:07:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fvets.2020.568249 Frontiers in Veterinary Science, v. 7. 2297-1769 http://hdl.handle.net/11449/208152 10.3389/fvets.2020.568249 2-s2.0-85096223728 |
url |
http://dx.doi.org/10.3389/fvets.2020.568249 http://hdl.handle.net/11449/208152 |
identifier_str_mv |
Frontiers in Veterinary Science, v. 7. 2297-1769 10.3389/fvets.2020.568249 2-s2.0-85096223728 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Veterinary Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129294804713472 |