Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus)
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-736X2016000200008 http://hdl.handle.net/11449/172833 |
Resumo: | Wounds of large tracts with loss of tissue viability and delayed healing by secondary intention are cases where it is necessary the use of reconstructive surgical techniques. The platelet rich plasma (PRP) is a product with a higher platelet concentration, adjuvant in the healing process of reconstructive surgeries, assisting in the processes of hemostasis and stimulation of angiogenesis. Thus, a study was devised to assess the efficacy of the gel produced from the platelet rich plasma (PRP) in the forward axial flaps thoracodorsal pattern in rabbits to evaluate the ability to promote the integration of the flap the recipient bed. We used 30 white rabbits New Zealand, separated into two groups of 15 animals, comprising the platelet rich plasma groups (GPRP), in which we used the gel before the synthesis of the surgical wound , and control (CG), in which only saline was used. To obtain the PRP, the blood was collected from the animals, was determined and the platelet count before preparation of the gel. At the beginning and end of the experiment the animals were weighed for analysis of average weight gain. After surgery began macroscopic in the 3rd, 7th and 14th day reviews, and we assessed the presence or absence of exudate, skin integrity, edema, redness and necrosis. After this step, yielded the material for the manufacture of surgical wound, followed by microscopic histological slides. We evaluated vascular proliferation, presence of polymorphonuclear and mononuclear cells, fibroblast proliferation, collagen deposition, reepithelialization and hemorrhage. The data were subjected to statistical analysis (Student t test, t paired, and Kruskal Wallis test, and p<0.05). The average weight gain was not significant between the groups; platelet concentration in the final sample of PRP was significantly higher when compared with the original; exudate and necrosis were significantly higher in the control group compared to PRP group, vascular proliferation and reepithelialization were significantly greater in PRP group, whereas the presence of mononuclear cells, polymorphonuclear cells, proliferation of fibroblasts, collagen and hemorrhage were not significant between groups. Obtained on the third day, as the difference significantly greater exudate variable in the control group compared to the PRP group; on the seventh day exudate and flushing were significantly higher in the control group, and the fourteenth day exudate and integrity of the skin were significantly higher in the control group. The results of this study showed that the use of platelet rich plasma gel in the form of reconstructive surgery was able to stimulate angiogenesis in the wound favoring the healing process. |
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Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus)Use of platelet rich plasma to stimulate angiogenesis in the thoracodorsal axial pattern flap in rabbits (Oryctolagus cuniculus)CicatrizationPRPRabbitReconstructive surgeryRevascularizationThoracodorsal axial pattern flapWoundWounds of large tracts with loss of tissue viability and delayed healing by secondary intention are cases where it is necessary the use of reconstructive surgical techniques. The platelet rich plasma (PRP) is a product with a higher platelet concentration, adjuvant in the healing process of reconstructive surgeries, assisting in the processes of hemostasis and stimulation of angiogenesis. Thus, a study was devised to assess the efficacy of the gel produced from the platelet rich plasma (PRP) in the forward axial flaps thoracodorsal pattern in rabbits to evaluate the ability to promote the integration of the flap the recipient bed. We used 30 white rabbits New Zealand, separated into two groups of 15 animals, comprising the platelet rich plasma groups (GPRP), in which we used the gel before the synthesis of the surgical wound , and control (CG), in which only saline was used. To obtain the PRP, the blood was collected from the animals, was determined and the platelet count before preparation of the gel. At the beginning and end of the experiment the animals were weighed for analysis of average weight gain. After surgery began macroscopic in the 3rd, 7th and 14th day reviews, and we assessed the presence or absence of exudate, skin integrity, edema, redness and necrosis. After this step, yielded the material for the manufacture of surgical wound, followed by microscopic histological slides. We evaluated vascular proliferation, presence of polymorphonuclear and mononuclear cells, fibroblast proliferation, collagen deposition, reepithelialization and hemorrhage. The data were subjected to statistical analysis (Student t test, t paired, and Kruskal Wallis test, and p<0.05). The average weight gain was not significant between the groups; platelet concentration in the final sample of PRP was significantly higher when compared with the original; exudate and necrosis were significantly higher in the control group compared to PRP group, vascular proliferation and reepithelialization were significantly greater in PRP group, whereas the presence of mononuclear cells, polymorphonuclear cells, proliferation of fibroblasts, collagen and hemorrhage were not significant between groups. Obtained on the third day, as the difference significantly greater exudate variable in the control group compared to the PRP group; on the seventh day exudate and flushing were significantly higher in the control group, and the fourteenth day exudate and integrity of the skin were significantly higher in the control group. The results of this study showed that the use of platelet rich plasma gel in the form of reconstructive surgery was able to stimulate angiogenesis in the wound favoring the healing process.Departamento de Clínica e Cirurgia Veterinária Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal (FCAV) Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/nDepartamento de Estatística FCAV-Unesp, Via de Acesso Professor Paulo Donato Castellane s/nDepartamento de Clínica e Cirurgia Veterinária Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal (FCAV) Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/nDepartamento de Estatística FCAV-Unesp, Via de Acesso Professor Paulo Donato Castellane s/nUniversidade Estadual Paulista (Unesp)Pazzini, Josiane M. [UNESP]De Nardi, Andrigo B. [UNESP]Huppes, Rafael R. [UNESP]Gering, Ana P. [UNESP]Ferreira, Marília G.P.A. [UNESP]Silveira, Camila P.B. [UNESP]Luzzi, Mayara C. [UNESP]Oliveira, João A. [UNESP]2018-12-11T17:02:21Z2018-12-11T17:02:21Z2016-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article108-118application/pdfhttp://dx.doi.org/10.1590/S0100-736X2016000200008Pesquisa Veterinaria Brasileira, v. 36, n. 2, p. 108-118, 2016.1678-51500100-736Xhttp://hdl.handle.net/11449/17283310.1590/S0100-736X2016000200008S0100-736X20160002001082-s2.0-84963755287S0100-736X2016000200108.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2024-06-06T14:09:12Zoai:repositorio.unesp.br:11449/172833Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:30:17.763870Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus) Use of platelet rich plasma to stimulate angiogenesis in the thoracodorsal axial pattern flap in rabbits (Oryctolagus cuniculus) |
title |
Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus) |
spellingShingle |
Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus) Pazzini, Josiane M. [UNESP] Cicatrization PRP Rabbit Reconstructive surgery Revascularization Thoracodorsal axial pattern flap Wound |
title_short |
Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus) |
title_full |
Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus) |
title_fullStr |
Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus) |
title_full_unstemmed |
Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus) |
title_sort |
Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus) |
author |
Pazzini, Josiane M. [UNESP] |
author_facet |
Pazzini, Josiane M. [UNESP] De Nardi, Andrigo B. [UNESP] Huppes, Rafael R. [UNESP] Gering, Ana P. [UNESP] Ferreira, Marília G.P.A. [UNESP] Silveira, Camila P.B. [UNESP] Luzzi, Mayara C. [UNESP] Oliveira, João A. [UNESP] |
author_role |
author |
author2 |
De Nardi, Andrigo B. [UNESP] Huppes, Rafael R. [UNESP] Gering, Ana P. [UNESP] Ferreira, Marília G.P.A. [UNESP] Silveira, Camila P.B. [UNESP] Luzzi, Mayara C. [UNESP] Oliveira, João A. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pazzini, Josiane M. [UNESP] De Nardi, Andrigo B. [UNESP] Huppes, Rafael R. [UNESP] Gering, Ana P. [UNESP] Ferreira, Marília G.P.A. [UNESP] Silveira, Camila P.B. [UNESP] Luzzi, Mayara C. [UNESP] Oliveira, João A. [UNESP] |
dc.subject.por.fl_str_mv |
Cicatrization PRP Rabbit Reconstructive surgery Revascularization Thoracodorsal axial pattern flap Wound |
topic |
Cicatrization PRP Rabbit Reconstructive surgery Revascularization Thoracodorsal axial pattern flap Wound |
description |
Wounds of large tracts with loss of tissue viability and delayed healing by secondary intention are cases where it is necessary the use of reconstructive surgical techniques. The platelet rich plasma (PRP) is a product with a higher platelet concentration, adjuvant in the healing process of reconstructive surgeries, assisting in the processes of hemostasis and stimulation of angiogenesis. Thus, a study was devised to assess the efficacy of the gel produced from the platelet rich plasma (PRP) in the forward axial flaps thoracodorsal pattern in rabbits to evaluate the ability to promote the integration of the flap the recipient bed. We used 30 white rabbits New Zealand, separated into two groups of 15 animals, comprising the platelet rich plasma groups (GPRP), in which we used the gel before the synthesis of the surgical wound , and control (CG), in which only saline was used. To obtain the PRP, the blood was collected from the animals, was determined and the platelet count before preparation of the gel. At the beginning and end of the experiment the animals were weighed for analysis of average weight gain. After surgery began macroscopic in the 3rd, 7th and 14th day reviews, and we assessed the presence or absence of exudate, skin integrity, edema, redness and necrosis. After this step, yielded the material for the manufacture of surgical wound, followed by microscopic histological slides. We evaluated vascular proliferation, presence of polymorphonuclear and mononuclear cells, fibroblast proliferation, collagen deposition, reepithelialization and hemorrhage. The data were subjected to statistical analysis (Student t test, t paired, and Kruskal Wallis test, and p<0.05). The average weight gain was not significant between the groups; platelet concentration in the final sample of PRP was significantly higher when compared with the original; exudate and necrosis were significantly higher in the control group compared to PRP group, vascular proliferation and reepithelialization were significantly greater in PRP group, whereas the presence of mononuclear cells, polymorphonuclear cells, proliferation of fibroblasts, collagen and hemorrhage were not significant between groups. Obtained on the third day, as the difference significantly greater exudate variable in the control group compared to the PRP group; on the seventh day exudate and flushing were significantly higher in the control group, and the fourteenth day exudate and integrity of the skin were significantly higher in the control group. The results of this study showed that the use of platelet rich plasma gel in the form of reconstructive surgery was able to stimulate angiogenesis in the wound favoring the healing process. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-01 2018-12-11T17:02:21Z 2018-12-11T17:02:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-736X2016000200008 Pesquisa Veterinaria Brasileira, v. 36, n. 2, p. 108-118, 2016. 1678-5150 0100-736X http://hdl.handle.net/11449/172833 10.1590/S0100-736X2016000200008 S0100-736X2016000200108 2-s2.0-84963755287 S0100-736X2016000200108.pdf |
url |
http://dx.doi.org/10.1590/S0100-736X2016000200008 http://hdl.handle.net/11449/172833 |
identifier_str_mv |
Pesquisa Veterinaria Brasileira, v. 36, n. 2, p. 108-118, 2016. 1678-5150 0100-736X 10.1590/S0100-736X2016000200008 S0100-736X2016000200108 2-s2.0-84963755287 S0100-736X2016000200108.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Pesquisa Veterinaria Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
108-118 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128522372251648 |