Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus)

Detalhes bibliográficos
Autor(a) principal: Pazzini, Josiane M. [UNESP]
Data de Publicação: 2016
Outros Autores: De Nardi, Andrigo B. [UNESP], Huppes, Rafael R. [UNESP], Gering, Ana P. [UNESP], Ferreira, Marília G.P.A. [UNESP], Silveira, Camila P.B. [UNESP], Luzzi, Mayara C. [UNESP], Oliveira, João A. [UNESP]
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-736X2016000200008
http://hdl.handle.net/11449/172833
Resumo: Wounds of large tracts with loss of tissue viability and delayed healing by secondary intention are cases where it is necessary the use of reconstructive surgical techniques. The platelet rich plasma (PRP) is a product with a higher platelet concentration, adjuvant in the healing process of reconstructive surgeries, assisting in the processes of hemostasis and stimulation of angiogenesis. Thus, a study was devised to assess the efficacy of the gel produced from the platelet rich plasma (PRP) in the forward axial flaps thoracodorsal pattern in rabbits to evaluate the ability to promote the integration of the flap the recipient bed. We used 30 white rabbits New Zealand, separated into two groups of 15 animals, comprising the platelet rich plasma groups (GPRP), in which we used the gel before the synthesis of the surgical wound , and control (CG), in which only saline was used. To obtain the PRP, the blood was collected from the animals, was determined and the platelet count before preparation of the gel. At the beginning and end of the experiment the animals were weighed for analysis of average weight gain. After surgery began macroscopic in the 3rd, 7th and 14th day reviews, and we assessed the presence or absence of exudate, skin integrity, edema, redness and necrosis. After this step, yielded the material for the manufacture of surgical wound, followed by microscopic histological slides. We evaluated vascular proliferation, presence of polymorphonuclear and mononuclear cells, fibroblast proliferation, collagen deposition, reepithelialization and hemorrhage. The data were subjected to statistical analysis (Student t test, t paired, and Kruskal Wallis test, and p<0.05). The average weight gain was not significant between the groups; platelet concentration in the final sample of PRP was significantly higher when compared with the original; exudate and necrosis were significantly higher in the control group compared to PRP group, vascular proliferation and reepithelialization were significantly greater in PRP group, whereas the presence of mononuclear cells, polymorphonuclear cells, proliferation of fibroblasts, collagen and hemorrhage were not significant between groups. Obtained on the third day, as the difference significantly greater exudate variable in the control group compared to the PRP group; on the seventh day exudate and flushing were significantly higher in the control group, and the fourteenth day exudate and integrity of the skin were significantly higher in the control group. The results of this study showed that the use of platelet rich plasma gel in the form of reconstructive surgery was able to stimulate angiogenesis in the wound favoring the healing process.
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spelling Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus)Use of platelet rich plasma to stimulate angiogenesis in the thoracodorsal axial pattern flap in rabbits (Oryctolagus cuniculus)CicatrizationPRPRabbitReconstructive surgeryRevascularizationThoracodorsal axial pattern flapWoundWounds of large tracts with loss of tissue viability and delayed healing by secondary intention are cases where it is necessary the use of reconstructive surgical techniques. The platelet rich plasma (PRP) is a product with a higher platelet concentration, adjuvant in the healing process of reconstructive surgeries, assisting in the processes of hemostasis and stimulation of angiogenesis. Thus, a study was devised to assess the efficacy of the gel produced from the platelet rich plasma (PRP) in the forward axial flaps thoracodorsal pattern in rabbits to evaluate the ability to promote the integration of the flap the recipient bed. We used 30 white rabbits New Zealand, separated into two groups of 15 animals, comprising the platelet rich plasma groups (GPRP), in which we used the gel before the synthesis of the surgical wound , and control (CG), in which only saline was used. To obtain the PRP, the blood was collected from the animals, was determined and the platelet count before preparation of the gel. At the beginning and end of the experiment the animals were weighed for analysis of average weight gain. After surgery began macroscopic in the 3rd, 7th and 14th day reviews, and we assessed the presence or absence of exudate, skin integrity, edema, redness and necrosis. After this step, yielded the material for the manufacture of surgical wound, followed by microscopic histological slides. We evaluated vascular proliferation, presence of polymorphonuclear and mononuclear cells, fibroblast proliferation, collagen deposition, reepithelialization and hemorrhage. The data were subjected to statistical analysis (Student t test, t paired, and Kruskal Wallis test, and p<0.05). The average weight gain was not significant between the groups; platelet concentration in the final sample of PRP was significantly higher when compared with the original; exudate and necrosis were significantly higher in the control group compared to PRP group, vascular proliferation and reepithelialization were significantly greater in PRP group, whereas the presence of mononuclear cells, polymorphonuclear cells, proliferation of fibroblasts, collagen and hemorrhage were not significant between groups. Obtained on the third day, as the difference significantly greater exudate variable in the control group compared to the PRP group; on the seventh day exudate and flushing were significantly higher in the control group, and the fourteenth day exudate and integrity of the skin were significantly higher in the control group. The results of this study showed that the use of platelet rich plasma gel in the form of reconstructive surgery was able to stimulate angiogenesis in the wound favoring the healing process.Departamento de Clínica e Cirurgia Veterinária Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal (FCAV) Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/nDepartamento de Estatística FCAV-Unesp, Via de Acesso Professor Paulo Donato Castellane s/nDepartamento de Clínica e Cirurgia Veterinária Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal (FCAV) Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/nDepartamento de Estatística FCAV-Unesp, Via de Acesso Professor Paulo Donato Castellane s/nUniversidade Estadual Paulista (Unesp)Pazzini, Josiane M. [UNESP]De Nardi, Andrigo B. [UNESP]Huppes, Rafael R. [UNESP]Gering, Ana P. [UNESP]Ferreira, Marília G.P.A. [UNESP]Silveira, Camila P.B. [UNESP]Luzzi, Mayara C. [UNESP]Oliveira, João A. [UNESP]2018-12-11T17:02:21Z2018-12-11T17:02:21Z2016-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article108-118application/pdfhttp://dx.doi.org/10.1590/S0100-736X2016000200008Pesquisa Veterinaria Brasileira, v. 36, n. 2, p. 108-118, 2016.1678-51500100-736Xhttp://hdl.handle.net/11449/17283310.1590/S0100-736X2016000200008S0100-736X20160002001082-s2.0-84963755287S0100-736X2016000200108.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2024-06-06T14:09:12Zoai:repositorio.unesp.br:11449/172833Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:30:17.763870Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus)
Use of platelet rich plasma to stimulate angiogenesis in the thoracodorsal axial pattern flap in rabbits (Oryctolagus cuniculus)
title Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus)
spellingShingle Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus)
Pazzini, Josiane M. [UNESP]
Cicatrization
PRP
Rabbit
Reconstructive surgery
Revascularization
Thoracodorsal axial pattern flap
Wound
title_short Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus)
title_full Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus)
title_fullStr Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus)
title_full_unstemmed Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus)
title_sort Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus)
author Pazzini, Josiane M. [UNESP]
author_facet Pazzini, Josiane M. [UNESP]
De Nardi, Andrigo B. [UNESP]
Huppes, Rafael R. [UNESP]
Gering, Ana P. [UNESP]
Ferreira, Marília G.P.A. [UNESP]
Silveira, Camila P.B. [UNESP]
Luzzi, Mayara C. [UNESP]
Oliveira, João A. [UNESP]
author_role author
author2 De Nardi, Andrigo B. [UNESP]
Huppes, Rafael R. [UNESP]
Gering, Ana P. [UNESP]
Ferreira, Marília G.P.A. [UNESP]
Silveira, Camila P.B. [UNESP]
Luzzi, Mayara C. [UNESP]
Oliveira, João A. [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Pazzini, Josiane M. [UNESP]
De Nardi, Andrigo B. [UNESP]
Huppes, Rafael R. [UNESP]
Gering, Ana P. [UNESP]
Ferreira, Marília G.P.A. [UNESP]
Silveira, Camila P.B. [UNESP]
Luzzi, Mayara C. [UNESP]
Oliveira, João A. [UNESP]
dc.subject.por.fl_str_mv Cicatrization
PRP
Rabbit
Reconstructive surgery
Revascularization
Thoracodorsal axial pattern flap
Wound
topic Cicatrization
PRP
Rabbit
Reconstructive surgery
Revascularization
Thoracodorsal axial pattern flap
Wound
description Wounds of large tracts with loss of tissue viability and delayed healing by secondary intention are cases where it is necessary the use of reconstructive surgical techniques. The platelet rich plasma (PRP) is a product with a higher platelet concentration, adjuvant in the healing process of reconstructive surgeries, assisting in the processes of hemostasis and stimulation of angiogenesis. Thus, a study was devised to assess the efficacy of the gel produced from the platelet rich plasma (PRP) in the forward axial flaps thoracodorsal pattern in rabbits to evaluate the ability to promote the integration of the flap the recipient bed. We used 30 white rabbits New Zealand, separated into two groups of 15 animals, comprising the platelet rich plasma groups (GPRP), in which we used the gel before the synthesis of the surgical wound , and control (CG), in which only saline was used. To obtain the PRP, the blood was collected from the animals, was determined and the platelet count before preparation of the gel. At the beginning and end of the experiment the animals were weighed for analysis of average weight gain. After surgery began macroscopic in the 3rd, 7th and 14th day reviews, and we assessed the presence or absence of exudate, skin integrity, edema, redness and necrosis. After this step, yielded the material for the manufacture of surgical wound, followed by microscopic histological slides. We evaluated vascular proliferation, presence of polymorphonuclear and mononuclear cells, fibroblast proliferation, collagen deposition, reepithelialization and hemorrhage. The data were subjected to statistical analysis (Student t test, t paired, and Kruskal Wallis test, and p<0.05). The average weight gain was not significant between the groups; platelet concentration in the final sample of PRP was significantly higher when compared with the original; exudate and necrosis were significantly higher in the control group compared to PRP group, vascular proliferation and reepithelialization were significantly greater in PRP group, whereas the presence of mononuclear cells, polymorphonuclear cells, proliferation of fibroblasts, collagen and hemorrhage were not significant between groups. Obtained on the third day, as the difference significantly greater exudate variable in the control group compared to the PRP group; on the seventh day exudate and flushing were significantly higher in the control group, and the fourteenth day exudate and integrity of the skin were significantly higher in the control group. The results of this study showed that the use of platelet rich plasma gel in the form of reconstructive surgery was able to stimulate angiogenesis in the wound favoring the healing process.
publishDate 2016
dc.date.none.fl_str_mv 2016-02-01
2018-12-11T17:02:21Z
2018-12-11T17:02:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-736X2016000200008
Pesquisa Veterinaria Brasileira, v. 36, n. 2, p. 108-118, 2016.
1678-5150
0100-736X
http://hdl.handle.net/11449/172833
10.1590/S0100-736X2016000200008
S0100-736X2016000200108
2-s2.0-84963755287
S0100-736X2016000200108.pdf
url http://dx.doi.org/10.1590/S0100-736X2016000200008
http://hdl.handle.net/11449/172833
identifier_str_mv Pesquisa Veterinaria Brasileira, v. 36, n. 2, p. 108-118, 2016.
1678-5150
0100-736X
10.1590/S0100-736X2016000200008
S0100-736X2016000200108
2-s2.0-84963755287
S0100-736X2016000200108.pdf
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Pesquisa Veterinaria Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 108-118
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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