Karyotypic diversity in four species of the genus Gymnotus Linnaeus, 1758 (Teleostei, Gymnotiformes, Gymnotidae): Physical mapping of ribosomal genes and telomeric sequences
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3897/compcytogen.v5i3.1375 http://hdl.handle.net/11449/226503 |
Resumo: | Conventional (Giemsa, C-Banding, Ag-NORs, CMA3) and molecular (5S rDNA, 18S rDNA, telomeric sequences) cytogenetic studies were carried out in specimens of ten distinct fish populations of the genus Gymnotus (G. sylvius Albert and Fernandes-Matioli, 1999, G. inaequilabiatus Valenciennes, 1839, G. pantherinus Steindachner, 1908, and G. cf. carapo Linnaeus, 1758) from different Brazilian hydrographic basins. G. sylvius presented a diploid number of 40 chromosomes (22m+12sm+6st), G. pantherinus presented 52 chromosomes (32m+18sm+2st), while G. inaequilabiatus (42m+10sm+2a) and G. cf. carapo (38m+12sm+4st) presented 54 chromosomes. The C-banding technique revealed centromeric marks in all chromosomes of all species. Besides that, conspicuous blocks of heterochromatin were found interstitially on the chromosomes of G. inaequilabiatus, G. cf. carapo, and G. pantherinus. All four species showed single nucleolus organizing regions confirmed by results obtained through Ag-NORs and FISH experiments using 18S rDNA probes, which showed the NORs localized on the first chromosome pair in G. inaequilabiatus, G. cf. carapo, and G. pantherinus, and on pair 2 in G. sylvius. CMA3 staining revealed additional unrelated NORs marks in G. sylvius and G. pantherinus. The 5S rDNA probes revealed signals on one pair in G. sylvius and two pairs in G. pantherinus; G. inaequilabiatus had about seventeen pairs marked, and G. cf. carapo had about fifteen pairs marked. It is considered that the high amount of heterochromatin identified in the chromosomes of G. inaequilabiatus and G. cf. carapo could have facilitated the dispersion of 5S rDNA in these species. Interstitial signals were detected on the first metacentric pair of G. sylvius by telomeric probes (TTAGGG)n indicating the possible occurrence of chromosomal fusions in this species. The present study reveals valuable cytotaxonomic markers for this group and allows a more precise evaluation of the processes involved in the karyotype differentiation and the interrelationships among different species of the genus Gymnotus. © P.C. Scacchetti et al. |
id |
UNSP_bf76adf1df258da12eaf1c6578466245 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/226503 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Karyotypic diversity in four species of the genus Gymnotus Linnaeus, 1758 (Teleostei, Gymnotiformes, Gymnotidae): Physical mapping of ribosomal genes and telomeric sequencesChromosomal rearrangementsCytogeneticsFISHHeterochromatinrDNAConventional (Giemsa, C-Banding, Ag-NORs, CMA3) and molecular (5S rDNA, 18S rDNA, telomeric sequences) cytogenetic studies were carried out in specimens of ten distinct fish populations of the genus Gymnotus (G. sylvius Albert and Fernandes-Matioli, 1999, G. inaequilabiatus Valenciennes, 1839, G. pantherinus Steindachner, 1908, and G. cf. carapo Linnaeus, 1758) from different Brazilian hydrographic basins. G. sylvius presented a diploid number of 40 chromosomes (22m+12sm+6st), G. pantherinus presented 52 chromosomes (32m+18sm+2st), while G. inaequilabiatus (42m+10sm+2a) and G. cf. carapo (38m+12sm+4st) presented 54 chromosomes. The C-banding technique revealed centromeric marks in all chromosomes of all species. Besides that, conspicuous blocks of heterochromatin were found interstitially on the chromosomes of G. inaequilabiatus, G. cf. carapo, and G. pantherinus. All four species showed single nucleolus organizing regions confirmed by results obtained through Ag-NORs and FISH experiments using 18S rDNA probes, which showed the NORs localized on the first chromosome pair in G. inaequilabiatus, G. cf. carapo, and G. pantherinus, and on pair 2 in G. sylvius. CMA3 staining revealed additional unrelated NORs marks in G. sylvius and G. pantherinus. The 5S rDNA probes revealed signals on one pair in G. sylvius and two pairs in G. pantherinus; G. inaequilabiatus had about seventeen pairs marked, and G. cf. carapo had about fifteen pairs marked. It is considered that the high amount of heterochromatin identified in the chromosomes of G. inaequilabiatus and G. cf. carapo could have facilitated the dispersion of 5S rDNA in these species. Interstitial signals were detected on the first metacentric pair of G. sylvius by telomeric probes (TTAGGG)n indicating the possible occurrence of chromosomal fusions in this species. The present study reveals valuable cytotaxonomic markers for this group and allows a more precise evaluation of the processes involved in the karyotype differentiation and the interrelationships among different species of the genus Gymnotus. © P.C. Scacchetti et al.Laboratório de Biologia e Genética de Peixes Instituto de Biociências de Botucatu Universidade Estadual Paulista (UNESP) Departamento de Morfologia Distrito de Rubião Junior, Botucatu, SP, 18618-970Laboratório de Biologia e Genética de Peixes Instituto de Biociências de Botucatu Universidade Estadual Paulista (UNESP) Departamento de Morfologia Distrito de Rubião Junior, Botucatu, SP, 18618-970Universidade Estadual Paulista (UNESP)Scacchetti, Priscilla Cardim [UNESP]Pansonato-Alves, José Carlos [UNESP]Utsunomia, Ricardo [UNESP]Oliveira, Claudio [UNESP]Foresti, Fausto [UNESP]2022-04-29T00:49:42Z2022-04-29T00:49:42Z2011-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article223-235http://dx.doi.org/10.3897/compcytogen.v5i3.1375Comparative Cytogenetics, v. 5, n. 3, p. 223-235, 2011.1993-078X1993-0771http://hdl.handle.net/11449/22650310.3897/compcytogen.v5i3.13752-s2.0-80052276111Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Cytogeneticsinfo:eu-repo/semantics/openAccess2022-04-29T00:49:42Zoai:repositorio.unesp.br:11449/226503Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:27:44.710063Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Karyotypic diversity in four species of the genus Gymnotus Linnaeus, 1758 (Teleostei, Gymnotiformes, Gymnotidae): Physical mapping of ribosomal genes and telomeric sequences |
title |
Karyotypic diversity in four species of the genus Gymnotus Linnaeus, 1758 (Teleostei, Gymnotiformes, Gymnotidae): Physical mapping of ribosomal genes and telomeric sequences |
spellingShingle |
Karyotypic diversity in four species of the genus Gymnotus Linnaeus, 1758 (Teleostei, Gymnotiformes, Gymnotidae): Physical mapping of ribosomal genes and telomeric sequences Scacchetti, Priscilla Cardim [UNESP] Chromosomal rearrangements Cytogenetics FISH Heterochromatin rDNA |
title_short |
Karyotypic diversity in four species of the genus Gymnotus Linnaeus, 1758 (Teleostei, Gymnotiformes, Gymnotidae): Physical mapping of ribosomal genes and telomeric sequences |
title_full |
Karyotypic diversity in four species of the genus Gymnotus Linnaeus, 1758 (Teleostei, Gymnotiformes, Gymnotidae): Physical mapping of ribosomal genes and telomeric sequences |
title_fullStr |
Karyotypic diversity in four species of the genus Gymnotus Linnaeus, 1758 (Teleostei, Gymnotiformes, Gymnotidae): Physical mapping of ribosomal genes and telomeric sequences |
title_full_unstemmed |
Karyotypic diversity in four species of the genus Gymnotus Linnaeus, 1758 (Teleostei, Gymnotiformes, Gymnotidae): Physical mapping of ribosomal genes and telomeric sequences |
title_sort |
Karyotypic diversity in four species of the genus Gymnotus Linnaeus, 1758 (Teleostei, Gymnotiformes, Gymnotidae): Physical mapping of ribosomal genes and telomeric sequences |
author |
Scacchetti, Priscilla Cardim [UNESP] |
author_facet |
Scacchetti, Priscilla Cardim [UNESP] Pansonato-Alves, José Carlos [UNESP] Utsunomia, Ricardo [UNESP] Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
author_role |
author |
author2 |
Pansonato-Alves, José Carlos [UNESP] Utsunomia, Ricardo [UNESP] Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Scacchetti, Priscilla Cardim [UNESP] Pansonato-Alves, José Carlos [UNESP] Utsunomia, Ricardo [UNESP] Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
dc.subject.por.fl_str_mv |
Chromosomal rearrangements Cytogenetics FISH Heterochromatin rDNA |
topic |
Chromosomal rearrangements Cytogenetics FISH Heterochromatin rDNA |
description |
Conventional (Giemsa, C-Banding, Ag-NORs, CMA3) and molecular (5S rDNA, 18S rDNA, telomeric sequences) cytogenetic studies were carried out in specimens of ten distinct fish populations of the genus Gymnotus (G. sylvius Albert and Fernandes-Matioli, 1999, G. inaequilabiatus Valenciennes, 1839, G. pantherinus Steindachner, 1908, and G. cf. carapo Linnaeus, 1758) from different Brazilian hydrographic basins. G. sylvius presented a diploid number of 40 chromosomes (22m+12sm+6st), G. pantherinus presented 52 chromosomes (32m+18sm+2st), while G. inaequilabiatus (42m+10sm+2a) and G. cf. carapo (38m+12sm+4st) presented 54 chromosomes. The C-banding technique revealed centromeric marks in all chromosomes of all species. Besides that, conspicuous blocks of heterochromatin were found interstitially on the chromosomes of G. inaequilabiatus, G. cf. carapo, and G. pantherinus. All four species showed single nucleolus organizing regions confirmed by results obtained through Ag-NORs and FISH experiments using 18S rDNA probes, which showed the NORs localized on the first chromosome pair in G. inaequilabiatus, G. cf. carapo, and G. pantherinus, and on pair 2 in G. sylvius. CMA3 staining revealed additional unrelated NORs marks in G. sylvius and G. pantherinus. The 5S rDNA probes revealed signals on one pair in G. sylvius and two pairs in G. pantherinus; G. inaequilabiatus had about seventeen pairs marked, and G. cf. carapo had about fifteen pairs marked. It is considered that the high amount of heterochromatin identified in the chromosomes of G. inaequilabiatus and G. cf. carapo could have facilitated the dispersion of 5S rDNA in these species. Interstitial signals were detected on the first metacentric pair of G. sylvius by telomeric probes (TTAGGG)n indicating the possible occurrence of chromosomal fusions in this species. The present study reveals valuable cytotaxonomic markers for this group and allows a more precise evaluation of the processes involved in the karyotype differentiation and the interrelationships among different species of the genus Gymnotus. © P.C. Scacchetti et al. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 2022-04-29T00:49:42Z 2022-04-29T00:49:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3897/compcytogen.v5i3.1375 Comparative Cytogenetics, v. 5, n. 3, p. 223-235, 2011. 1993-078X 1993-0771 http://hdl.handle.net/11449/226503 10.3897/compcytogen.v5i3.1375 2-s2.0-80052276111 |
url |
http://dx.doi.org/10.3897/compcytogen.v5i3.1375 http://hdl.handle.net/11449/226503 |
identifier_str_mv |
Comparative Cytogenetics, v. 5, n. 3, p. 223-235, 2011. 1993-078X 1993-0771 10.3897/compcytogen.v5i3.1375 2-s2.0-80052276111 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Cytogenetics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
223-235 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129322758701056 |