Chemical characterization of Brazilian savannah Byrsonima species (muricis) and their impact on genomic instability and chemopreventive effects
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.mrgentox.2023.503586 http://hdl.handle.net/11449/248298 |
Resumo: | The identification of new drugs with few or no adverse effects is of great interest worldwide. In cancer therapy, natural products have been used as chemopreventive and chemotherapeutic agents. Plants from the Brazilian savannah belonging to the Byrsonima genus are popularly known as muricis and have attracted much attention due to their various pharmacological activities. However, there are currently no data on these plants concerning their use as chemopreventive or chemotherapeutic agents in human cell lines. The present study assessed the potential of B. correifolia, B. verbascifolia, B. crassifolia, and B. intermedia extracts as natural alternatives in the prevention and/or treatment of cancer. The chemical constituents present in each extract were analyzed by electrospray ionization–mass spectrometry (ESI-MSN). The mutagenic/antimutagenic (micronucleus assay), genotoxic/antigenotoxic (comet assay), apoptotic/necrotic (acridine orange/ethidium bromide uptake), and oxidative/antioxidative (CM-H2DCFDA) effects of the extracts and their influence on gene expression (RT[sbnd]qPCR) were investigated in nonmetabolizing gastric (MNP01) and metabolizing hepatocarcinoma (HepG2) epithelial cells to evaluate the effects of metabolism on the biological activities of the extracts. The genotoxicity, mutagenicity, and apoptotic effects observed in HepG2 cells with B. correifolia and B. verbascifolia extracts are probably associated with the presence of proanthocyanidins and amentoflavone. In MNP01 cells, none of the four extracts showed mutagenic effects. B. crassifolia and B. intermedia extracts exhibited strong antimutagenicity and enhanced detoxification in HepG2 cells and antioxidant capacities in both types of cells, possibly due to the presence of gallic and quinic acids, which possess chemopreventive properties. This study identifies for the first time B. correifolia and B. verbascifolia extracts as potential agents against hepatocarcinoma and B. crassifolia and B. intermedia extracts as putative chemopreventive agents. |
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Chemical characterization of Brazilian savannah Byrsonima species (muricis) and their impact on genomic instability and chemopreventive effectsAnticancerApoptosisin vitroMedicinal plantMutagenesisPhytochemicalsThe identification of new drugs with few or no adverse effects is of great interest worldwide. In cancer therapy, natural products have been used as chemopreventive and chemotherapeutic agents. Plants from the Brazilian savannah belonging to the Byrsonima genus are popularly known as muricis and have attracted much attention due to their various pharmacological activities. However, there are currently no data on these plants concerning their use as chemopreventive or chemotherapeutic agents in human cell lines. The present study assessed the potential of B. correifolia, B. verbascifolia, B. crassifolia, and B. intermedia extracts as natural alternatives in the prevention and/or treatment of cancer. The chemical constituents present in each extract were analyzed by electrospray ionization–mass spectrometry (ESI-MSN). The mutagenic/antimutagenic (micronucleus assay), genotoxic/antigenotoxic (comet assay), apoptotic/necrotic (acridine orange/ethidium bromide uptake), and oxidative/antioxidative (CM-H2DCFDA) effects of the extracts and their influence on gene expression (RT[sbnd]qPCR) were investigated in nonmetabolizing gastric (MNP01) and metabolizing hepatocarcinoma (HepG2) epithelial cells to evaluate the effects of metabolism on the biological activities of the extracts. The genotoxicity, mutagenicity, and apoptotic effects observed in HepG2 cells with B. correifolia and B. verbascifolia extracts are probably associated with the presence of proanthocyanidins and amentoflavone. In MNP01 cells, none of the four extracts showed mutagenic effects. B. crassifolia and B. intermedia extracts exhibited strong antimutagenicity and enhanced detoxification in HepG2 cells and antioxidant capacities in both types of cells, possibly due to the presence of gallic and quinic acids, which possess chemopreventive properties. This study identifies for the first time B. correifolia and B. verbascifolia extracts as potential agents against hepatocarcinoma and B. crassifolia and B. intermedia extracts as putative chemopreventive agents.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of General Biology State University of LondrinaDepartment of Chemistry São Paulo State University “Júlio de Mesquita Filho”School of Arts Science and Humanities São Paulo UniversityDepartment of Biological Sciences Faculty of Pharmaceutical Sciences of Araraquara São Paulo State University “Júlio de Mesquita Filho”Institute of Biosciences São Paulo State UniversityDepartment of Chemistry São Paulo State University “Júlio de Mesquita Filho”Department of Biological Sciences Faculty of Pharmaceutical Sciences of Araraquara São Paulo State University “Júlio de Mesquita Filho”Institute of Biosciences São Paulo State UniversityCAPES: 001FAPESP: 2009/52237-9Universidade Estadual de Londrina (UEL)Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Specian, Ana Flávia L.Tuttis, KatiuskaSerpeloni, Juliana M.Ribeiro, Diego L.Nunes, Higor LopesTangerina, Marcelo M.P. [UNESP]Sannomiya, MiriamVaranda, Eliana A. [UNESP]Vilegas, Wagner [UNESP]Cólus, Ilce Mara S.2023-07-29T13:39:58Z2023-07-29T13:39:58Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.mrgentox.2023.503586Mutation Research - Genetic Toxicology and Environmental Mutagenesis, v. 887.1879-35921383-5718http://hdl.handle.net/11449/24829810.1016/j.mrgentox.2023.5035862-s2.0-85147268302Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMutation Research - Genetic Toxicology and Environmental Mutagenesisinfo:eu-repo/semantics/openAccess2024-06-24T13:07:13Zoai:repositorio.unesp.br:11449/248298Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:11:11.671479Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chemical characterization of Brazilian savannah Byrsonima species (muricis) and their impact on genomic instability and chemopreventive effects |
title |
Chemical characterization of Brazilian savannah Byrsonima species (muricis) and their impact on genomic instability and chemopreventive effects |
spellingShingle |
Chemical characterization of Brazilian savannah Byrsonima species (muricis) and their impact on genomic instability and chemopreventive effects Specian, Ana Flávia L. Anticancer Apoptosis in vitro Medicinal plant Mutagenesis Phytochemicals |
title_short |
Chemical characterization of Brazilian savannah Byrsonima species (muricis) and their impact on genomic instability and chemopreventive effects |
title_full |
Chemical characterization of Brazilian savannah Byrsonima species (muricis) and their impact on genomic instability and chemopreventive effects |
title_fullStr |
Chemical characterization of Brazilian savannah Byrsonima species (muricis) and their impact on genomic instability and chemopreventive effects |
title_full_unstemmed |
Chemical characterization of Brazilian savannah Byrsonima species (muricis) and their impact on genomic instability and chemopreventive effects |
title_sort |
Chemical characterization of Brazilian savannah Byrsonima species (muricis) and their impact on genomic instability and chemopreventive effects |
author |
Specian, Ana Flávia L. |
author_facet |
Specian, Ana Flávia L. Tuttis, Katiuska Serpeloni, Juliana M. Ribeiro, Diego L. Nunes, Higor Lopes Tangerina, Marcelo M.P. [UNESP] Sannomiya, Miriam Varanda, Eliana A. [UNESP] Vilegas, Wagner [UNESP] Cólus, Ilce Mara S. |
author_role |
author |
author2 |
Tuttis, Katiuska Serpeloni, Juliana M. Ribeiro, Diego L. Nunes, Higor Lopes Tangerina, Marcelo M.P. [UNESP] Sannomiya, Miriam Varanda, Eliana A. [UNESP] Vilegas, Wagner [UNESP] Cólus, Ilce Mara S. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Specian, Ana Flávia L. Tuttis, Katiuska Serpeloni, Juliana M. Ribeiro, Diego L. Nunes, Higor Lopes Tangerina, Marcelo M.P. [UNESP] Sannomiya, Miriam Varanda, Eliana A. [UNESP] Vilegas, Wagner [UNESP] Cólus, Ilce Mara S. |
dc.subject.por.fl_str_mv |
Anticancer Apoptosis in vitro Medicinal plant Mutagenesis Phytochemicals |
topic |
Anticancer Apoptosis in vitro Medicinal plant Mutagenesis Phytochemicals |
description |
The identification of new drugs with few or no adverse effects is of great interest worldwide. In cancer therapy, natural products have been used as chemopreventive and chemotherapeutic agents. Plants from the Brazilian savannah belonging to the Byrsonima genus are popularly known as muricis and have attracted much attention due to their various pharmacological activities. However, there are currently no data on these plants concerning their use as chemopreventive or chemotherapeutic agents in human cell lines. The present study assessed the potential of B. correifolia, B. verbascifolia, B. crassifolia, and B. intermedia extracts as natural alternatives in the prevention and/or treatment of cancer. The chemical constituents present in each extract were analyzed by electrospray ionization–mass spectrometry (ESI-MSN). The mutagenic/antimutagenic (micronucleus assay), genotoxic/antigenotoxic (comet assay), apoptotic/necrotic (acridine orange/ethidium bromide uptake), and oxidative/antioxidative (CM-H2DCFDA) effects of the extracts and their influence on gene expression (RT[sbnd]qPCR) were investigated in nonmetabolizing gastric (MNP01) and metabolizing hepatocarcinoma (HepG2) epithelial cells to evaluate the effects of metabolism on the biological activities of the extracts. The genotoxicity, mutagenicity, and apoptotic effects observed in HepG2 cells with B. correifolia and B. verbascifolia extracts are probably associated with the presence of proanthocyanidins and amentoflavone. In MNP01 cells, none of the four extracts showed mutagenic effects. B. crassifolia and B. intermedia extracts exhibited strong antimutagenicity and enhanced detoxification in HepG2 cells and antioxidant capacities in both types of cells, possibly due to the presence of gallic and quinic acids, which possess chemopreventive properties. This study identifies for the first time B. correifolia and B. verbascifolia extracts as potential agents against hepatocarcinoma and B. crassifolia and B. intermedia extracts as putative chemopreventive agents. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:39:58Z 2023-07-29T13:39:58Z 2023-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.mrgentox.2023.503586 Mutation Research - Genetic Toxicology and Environmental Mutagenesis, v. 887. 1879-3592 1383-5718 http://hdl.handle.net/11449/248298 10.1016/j.mrgentox.2023.503586 2-s2.0-85147268302 |
url |
http://dx.doi.org/10.1016/j.mrgentox.2023.503586 http://hdl.handle.net/11449/248298 |
identifier_str_mv |
Mutation Research - Genetic Toxicology and Environmental Mutagenesis, v. 887. 1879-3592 1383-5718 10.1016/j.mrgentox.2023.503586 2-s2.0-85147268302 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mutation Research - Genetic Toxicology and Environmental Mutagenesis |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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_version_ |
1808128475866857472 |