Investigating the Modes of Action of the Antimicrobial Chalcones BC1 and T9A
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/molecules25204596 http://hdl.handle.net/11449/209614 |
Resumo: | Xanthomonas citri subsp. citri (X. citri) is an important phytopathogen and causes Asiatic Citrus Canker (ACC). To control ACC, copper sprays are commonly used. As copper is an environmentally damaging heavy metal, new antimicrobials are needed to combat citrus canker. Here, we explored the antimicrobial activity of chalcones, specifically the methoxychalcone BC1 and the hydroxychalcone T9A, against X. citri and the model organism Bacillus subtilis. BC1 and T9A prevented growth of X. citri and B. subtilis in concentrations varying from 20 mu g/mL to 40 mu g/mL. BC1 and T9A decreased incorporation of radiolabeled precursors of DNA, RNA, protein, and peptidoglycan in X. citri and B. subtilis. Both compounds mildly affected respiratory activity in X. citri, but T9A strongly decreased respiratory activity in B. subtilis. In line with that finding, intracellular ATP decreased strongly in B. subtilis upon T9A treatment, whereas BC1 increased intracellular ATP. In X. citri, both compounds resulted in a decrease in intracellular ATP. Cell division seems not to be affected in X. citri, and, although in B. subtilis the formation of FtsZ-rings is affected, a FtsZ GTPase activity assay suggests that this is an indirect effect. The chalcones studied here represent a sustainable alternative to copper for the control of ACC, and further studies are ongoing to elucidate their precise modes of action. |
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Investigating the Modes of Action of the Antimicrobial Chalcones BC1 and T9AchalconeAsiatic citrus cankerBacillus subtilisXanthomonas citriXanthomonas citri subsp. citri (X. citri) is an important phytopathogen and causes Asiatic Citrus Canker (ACC). To control ACC, copper sprays are commonly used. As copper is an environmentally damaging heavy metal, new antimicrobials are needed to combat citrus canker. Here, we explored the antimicrobial activity of chalcones, specifically the methoxychalcone BC1 and the hydroxychalcone T9A, against X. citri and the model organism Bacillus subtilis. BC1 and T9A prevented growth of X. citri and B. subtilis in concentrations varying from 20 mu g/mL to 40 mu g/mL. BC1 and T9A decreased incorporation of radiolabeled precursors of DNA, RNA, protein, and peptidoglycan in X. citri and B. subtilis. Both compounds mildly affected respiratory activity in X. citri, but T9A strongly decreased respiratory activity in B. subtilis. In line with that finding, intracellular ATP decreased strongly in B. subtilis upon T9A treatment, whereas BC1 increased intracellular ATP. In X. citri, both compounds resulted in a decrease in intracellular ATP. Cell division seems not to be affected in X. citri, and, although in B. subtilis the formation of FtsZ-rings is affected, a FtsZ GTPase activity assay suggests that this is an indirect effect. The chalcones studied here represent a sustainable alternative to copper for the control of ACC, and further studies are ongoing to elucidate their precise modes of action.Nederlandse Organisatie voor Wetenschappelijk OnderzoekFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Dept Bioquim & Microbiol, Inst Biociencias, BR-13050690 Rio Claro, SP, BrazilUniv Groningen, Dept Mol Microbiol, Groningen Biomol Sci & Biotechnol Inst, NL-9747 AG Groningen, NetherlandsUniv Estadual Paulista, Dept Quim & Ciencias Ambientais, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilUniv Estadual Paulista, Dept Bioquim & Microbiol, Inst Biociencias, BR-13050690 Rio Claro, SP, BrazilUniv Estadual Paulista, Dept Quim & Ciencias Ambientais, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilNederlandse Organisatie voor Wetenschappelijk Onderzoek: 729.004.005FAPESP: 2017/50216-0 ; 2014/18330-0; 2018/15083-2; 2014/18577-5; 2016/08084-7; 2014/18719-4; 2016/07108-0CNPq: 246986/2013-1; 471129/2013-5; 306251/2016-7; 429322/2018-6MdpiUniversidade Estadual Paulista (Unesp)Univ GroningenMorao, Luana G. [UNESP]Lorenzoni, Andre S. G.Chakraborty, ParichitaAyusso, Gabriela M. [UNESP]Cavalca, Lucia B.Santos, Mariana B. [UNESP]Marques, Beatriz C. [UNESP]Dilarri, Guilherme [UNESP]Zamuner, Caio [UNESP]Regasini, Luis O. [UNESP]Ferreira, Henrique [UNESP]Scheffers, Dirk-Jan2021-06-25T12:23:53Z2021-06-25T12:23:53Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14http://dx.doi.org/10.3390/molecules25204596Molecules. Basel: Mdpi, v. 25, n. 20, 14 p., 2020.http://hdl.handle.net/11449/20961410.3390/molecules25204596WOS:000585602400001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMoleculesinfo:eu-repo/semantics/openAccess2021-10-23T19:28:18Zoai:repositorio.unesp.br:11449/209614Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:55:03.478737Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Investigating the Modes of Action of the Antimicrobial Chalcones BC1 and T9A |
title |
Investigating the Modes of Action of the Antimicrobial Chalcones BC1 and T9A |
spellingShingle |
Investigating the Modes of Action of the Antimicrobial Chalcones BC1 and T9A Morao, Luana G. [UNESP] chalcone Asiatic citrus canker Bacillus subtilis Xanthomonas citri |
title_short |
Investigating the Modes of Action of the Antimicrobial Chalcones BC1 and T9A |
title_full |
Investigating the Modes of Action of the Antimicrobial Chalcones BC1 and T9A |
title_fullStr |
Investigating the Modes of Action of the Antimicrobial Chalcones BC1 and T9A |
title_full_unstemmed |
Investigating the Modes of Action of the Antimicrobial Chalcones BC1 and T9A |
title_sort |
Investigating the Modes of Action of the Antimicrobial Chalcones BC1 and T9A |
author |
Morao, Luana G. [UNESP] |
author_facet |
Morao, Luana G. [UNESP] Lorenzoni, Andre S. G. Chakraborty, Parichita Ayusso, Gabriela M. [UNESP] Cavalca, Lucia B. Santos, Mariana B. [UNESP] Marques, Beatriz C. [UNESP] Dilarri, Guilherme [UNESP] Zamuner, Caio [UNESP] Regasini, Luis O. [UNESP] Ferreira, Henrique [UNESP] Scheffers, Dirk-Jan |
author_role |
author |
author2 |
Lorenzoni, Andre S. G. Chakraborty, Parichita Ayusso, Gabriela M. [UNESP] Cavalca, Lucia B. Santos, Mariana B. [UNESP] Marques, Beatriz C. [UNESP] Dilarri, Guilherme [UNESP] Zamuner, Caio [UNESP] Regasini, Luis O. [UNESP] Ferreira, Henrique [UNESP] Scheffers, Dirk-Jan |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Groningen |
dc.contributor.author.fl_str_mv |
Morao, Luana G. [UNESP] Lorenzoni, Andre S. G. Chakraborty, Parichita Ayusso, Gabriela M. [UNESP] Cavalca, Lucia B. Santos, Mariana B. [UNESP] Marques, Beatriz C. [UNESP] Dilarri, Guilherme [UNESP] Zamuner, Caio [UNESP] Regasini, Luis O. [UNESP] Ferreira, Henrique [UNESP] Scheffers, Dirk-Jan |
dc.subject.por.fl_str_mv |
chalcone Asiatic citrus canker Bacillus subtilis Xanthomonas citri |
topic |
chalcone Asiatic citrus canker Bacillus subtilis Xanthomonas citri |
description |
Xanthomonas citri subsp. citri (X. citri) is an important phytopathogen and causes Asiatic Citrus Canker (ACC). To control ACC, copper sprays are commonly used. As copper is an environmentally damaging heavy metal, new antimicrobials are needed to combat citrus canker. Here, we explored the antimicrobial activity of chalcones, specifically the methoxychalcone BC1 and the hydroxychalcone T9A, against X. citri and the model organism Bacillus subtilis. BC1 and T9A prevented growth of X. citri and B. subtilis in concentrations varying from 20 mu g/mL to 40 mu g/mL. BC1 and T9A decreased incorporation of radiolabeled precursors of DNA, RNA, protein, and peptidoglycan in X. citri and B. subtilis. Both compounds mildly affected respiratory activity in X. citri, but T9A strongly decreased respiratory activity in B. subtilis. In line with that finding, intracellular ATP decreased strongly in B. subtilis upon T9A treatment, whereas BC1 increased intracellular ATP. In X. citri, both compounds resulted in a decrease in intracellular ATP. Cell division seems not to be affected in X. citri, and, although in B. subtilis the formation of FtsZ-rings is affected, a FtsZ GTPase activity assay suggests that this is an indirect effect. The chalcones studied here represent a sustainable alternative to copper for the control of ACC, and further studies are ongoing to elucidate their precise modes of action. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-01 2021-06-25T12:23:53Z 2021-06-25T12:23:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/molecules25204596 Molecules. Basel: Mdpi, v. 25, n. 20, 14 p., 2020. http://hdl.handle.net/11449/209614 10.3390/molecules25204596 WOS:000585602400001 |
url |
http://dx.doi.org/10.3390/molecules25204596 http://hdl.handle.net/11449/209614 |
identifier_str_mv |
Molecules. Basel: Mdpi, v. 25, n. 20, 14 p., 2020. 10.3390/molecules25204596 WOS:000585602400001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecules |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 |
dc.publisher.none.fl_str_mv |
Mdpi |
publisher.none.fl_str_mv |
Mdpi |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129263269838848 |