Low-temperature plasma: An effective approach against Candida albicans biofilm
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1615/PlasmaMed.2014012361 http://hdl.handle.net/11449/227927 |
Resumo: | This study evaluated the antifungal potential of low-temperature plasma (LTP) on a 72-hour Candida albicans biofilm. A growth inhibition zone test was conducted with agar plates inoculated with C. albicans and submitted to LTP and argon application at 3 and 10 mm for 10, 30, 60, 90, and 120 seconds. The groups for biofilm assays were 60 seconds of LTP application with a tip-to-sample distance of 3 mm (LTP-3) and 10 mm (LTP-10); –application of only argon gas for 60 seconds with a tip-to-sample distance of 3 mm (Ar-3) and 10 mm (Ar-10); and no treatment. The C. albicans biofilm was grown on saliva-coated discs. The medium was replaced every 24 hours. Confocal laser scanning microscopy revealed the proportion of live and dead cells, and variable pressure scanning electron microscopy (VPSEM) showed biofilm/cell structure. No inhibition zone was observed for control and either Ar groups. For the LTP groups, a progressively increasing of inhibition zone diameter was observed for different treatment durations. The LTP-3 and LTP-10 groups presented higher proportions of dead cells compared with the Ar-3 and Ar-10 groups. VPSEM revealed cell perforations in the LTP-3 and LTP-10 groups. A short period of LTP exposure demonstrated an antifungal effect on C. albicans biofilm. |
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Low-temperature plasma: An effective approach against Candida albicans biofilmCandida albicansLow-temperature plasmaOral biofilmThis study evaluated the antifungal potential of low-temperature plasma (LTP) on a 72-hour Candida albicans biofilm. A growth inhibition zone test was conducted with agar plates inoculated with C. albicans and submitted to LTP and argon application at 3 and 10 mm for 10, 30, 60, 90, and 120 seconds. The groups for biofilm assays were 60 seconds of LTP application with a tip-to-sample distance of 3 mm (LTP-3) and 10 mm (LTP-10); –application of only argon gas for 60 seconds with a tip-to-sample distance of 3 mm (Ar-3) and 10 mm (Ar-10); and no treatment. The C. albicans biofilm was grown on saliva-coated discs. The medium was replaced every 24 hours. Confocal laser scanning microscopy revealed the proportion of live and dead cells, and variable pressure scanning electron microscopy (VPSEM) showed biofilm/cell structure. No inhibition zone was observed for control and either Ar groups. For the LTP groups, a progressively increasing of inhibition zone diameter was observed for different treatment durations. The LTP-3 and LTP-10 groups presented higher proportions of dead cells compared with the Ar-3 and Ar-10 groups. VPSEM revealed cell perforations in the LTP-3 and LTP-10 groups. A short period of LTP exposure demonstrated an antifungal effect on C. albicans biofilm.Department of Dental Materials and Prosthodontics Araçatuba Dental School Sao Paulo State UniversityDepartment of Basic Science and Craniofacial Biology New York University College of DentistryDepartment of Biomaterials and Biomimetics New York University College of DentistryDepartment of Dental Materials and Prosthodontics Araçatuba Dental School Sao Paulo State UniversityUniversidade Estadual Paulista (UNESP)New York University College of DentistryDelben, Juliana Aparecida [UNESP]Murata, Ramiro MendonçaWei, XiCastro, Myrella LessioAssunção, Wirley Gonçalves [UNESP]da Silva, Nelson Renato França AlvesDuarte, Simone2022-04-29T07:25:51Z2022-04-29T07:25:51Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article231-244http://dx.doi.org/10.1615/PlasmaMed.2014012361Plasma Medicine, v. 4, n. 1-4, p. 231-244, 2014.1947-57721947-5764http://hdl.handle.net/11449/22792710.1615/PlasmaMed.20140123612-s2.0-84922708062Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlasma Medicineinfo:eu-repo/semantics/openAccess2022-04-29T07:25:51Zoai:repositorio.unesp.br:11449/227927Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T07:25:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Low-temperature plasma: An effective approach against Candida albicans biofilm |
title |
Low-temperature plasma: An effective approach against Candida albicans biofilm |
spellingShingle |
Low-temperature plasma: An effective approach against Candida albicans biofilm Delben, Juliana Aparecida [UNESP] Candida albicans Low-temperature plasma Oral biofilm |
title_short |
Low-temperature plasma: An effective approach against Candida albicans biofilm |
title_full |
Low-temperature plasma: An effective approach against Candida albicans biofilm |
title_fullStr |
Low-temperature plasma: An effective approach against Candida albicans biofilm |
title_full_unstemmed |
Low-temperature plasma: An effective approach against Candida albicans biofilm |
title_sort |
Low-temperature plasma: An effective approach against Candida albicans biofilm |
author |
Delben, Juliana Aparecida [UNESP] |
author_facet |
Delben, Juliana Aparecida [UNESP] Murata, Ramiro Mendonça Wei, Xi Castro, Myrella Lessio Assunção, Wirley Gonçalves [UNESP] da Silva, Nelson Renato França Alves Duarte, Simone |
author_role |
author |
author2 |
Murata, Ramiro Mendonça Wei, Xi Castro, Myrella Lessio Assunção, Wirley Gonçalves [UNESP] da Silva, Nelson Renato França Alves Duarte, Simone |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) New York University College of Dentistry |
dc.contributor.author.fl_str_mv |
Delben, Juliana Aparecida [UNESP] Murata, Ramiro Mendonça Wei, Xi Castro, Myrella Lessio Assunção, Wirley Gonçalves [UNESP] da Silva, Nelson Renato França Alves Duarte, Simone |
dc.subject.por.fl_str_mv |
Candida albicans Low-temperature plasma Oral biofilm |
topic |
Candida albicans Low-temperature plasma Oral biofilm |
description |
This study evaluated the antifungal potential of low-temperature plasma (LTP) on a 72-hour Candida albicans biofilm. A growth inhibition zone test was conducted with agar plates inoculated with C. albicans and submitted to LTP and argon application at 3 and 10 mm for 10, 30, 60, 90, and 120 seconds. The groups for biofilm assays were 60 seconds of LTP application with a tip-to-sample distance of 3 mm (LTP-3) and 10 mm (LTP-10); –application of only argon gas for 60 seconds with a tip-to-sample distance of 3 mm (Ar-3) and 10 mm (Ar-10); and no treatment. The C. albicans biofilm was grown on saliva-coated discs. The medium was replaced every 24 hours. Confocal laser scanning microscopy revealed the proportion of live and dead cells, and variable pressure scanning electron microscopy (VPSEM) showed biofilm/cell structure. No inhibition zone was observed for control and either Ar groups. For the LTP groups, a progressively increasing of inhibition zone diameter was observed for different treatment durations. The LTP-3 and LTP-10 groups presented higher proportions of dead cells compared with the Ar-3 and Ar-10 groups. VPSEM revealed cell perforations in the LTP-3 and LTP-10 groups. A short period of LTP exposure demonstrated an antifungal effect on C. albicans biofilm. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 2022-04-29T07:25:51Z 2022-04-29T07:25:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1615/PlasmaMed.2014012361 Plasma Medicine, v. 4, n. 1-4, p. 231-244, 2014. 1947-5772 1947-5764 http://hdl.handle.net/11449/227927 10.1615/PlasmaMed.2014012361 2-s2.0-84922708062 |
url |
http://dx.doi.org/10.1615/PlasmaMed.2014012361 http://hdl.handle.net/11449/227927 |
identifier_str_mv |
Plasma Medicine, v. 4, n. 1-4, p. 231-244, 2014. 1947-5772 1947-5764 10.1615/PlasmaMed.2014012361 2-s2.0-84922708062 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plasma Medicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
231-244 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965069374652416 |