Live and ultrasound-inactivated Lacticaseibacillus casei modulate the intestinal microbiota and improve biochemical and cardiovascular parameters in male rats fed a high-fat diet

Detalhes bibliográficos
Autor(a) principal: Brandao, Larissa Ramalho
Data de Publicação: 2021
Outros Autores: Brito Alves, Jose Luiz de, Costa, Whyara Karoline Almeida da, Ferreira, Georgianna de Araujo Henriques, Oliveira, Matthaws Pereira de, Gomes da Cruz, Adriano, Braga, Valdir de Andrade, Aquino, Jailane de Souza, Vidal, Hubert, Noronha, Melline Fontes, Cabral, Lucelia [UNESP], Pimentel, Tatiana Colombo, Magnani, Marciane
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1039/d1fo01064f
http://hdl.handle.net/11449/210346
Resumo: This study aimed to evaluate the effects of ingestion of live (9 log CFU mL(-1)) and ultrasound-inactivated (paraprobiotic, 20 kHz, 40 min) Lacticaseibacillus casei 01 cells for 28 days on healthy parameters (biochemical and cardiovascular) and intestinal microbiota (amplicon sequencing of 16S ribosomal RNA) of rats fed a high-fat diet. Twenty-four male Wistar rats were divided into four groups of six animals: CTL (standard diet), HFD (high-fat diet), HFD-LC (high-fat diet and live L. casei), and HFD-ILC (high-fat diet and inactivated L. casei). The administration of live and ultrasound-inactivated L. casei prevented the increase (p < 0.05) in cholesterol levels (total and LDL) and controlled the insulin resistance in rats fed a high-fat diet. Furthermore, it promoted a modulation of the intestinal microbial composition by increasing (p < 0.05) beneficial bacteria (Lachnospiraceae and Ruminoccocaceae) and decreasing (p < 0.05) harmful bacteria (Clostridiaceae, Enterobacteriaceae, and Helicobacteriacea), attenuating the effects promoted by the HFD ingestion. Only live cells could increase (p < 0.05) the HDL-cholesterol, while only inactivated cells caused attenuation (p < 0.05) of the blood pressure. Results show beneficial effects of live and inactivated L. casei 01 and indicate that ultrasound inactivation produces a paraprobiotic with similar or improved health properties compared to live cells.
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spelling Live and ultrasound-inactivated Lacticaseibacillus casei modulate the intestinal microbiota and improve biochemical and cardiovascular parameters in male rats fed a high-fat dietThis study aimed to evaluate the effects of ingestion of live (9 log CFU mL(-1)) and ultrasound-inactivated (paraprobiotic, 20 kHz, 40 min) Lacticaseibacillus casei 01 cells for 28 days on healthy parameters (biochemical and cardiovascular) and intestinal microbiota (amplicon sequencing of 16S ribosomal RNA) of rats fed a high-fat diet. Twenty-four male Wistar rats were divided into four groups of six animals: CTL (standard diet), HFD (high-fat diet), HFD-LC (high-fat diet and live L. casei), and HFD-ILC (high-fat diet and inactivated L. casei). The administration of live and ultrasound-inactivated L. casei prevented the increase (p < 0.05) in cholesterol levels (total and LDL) and controlled the insulin resistance in rats fed a high-fat diet. Furthermore, it promoted a modulation of the intestinal microbial composition by increasing (p < 0.05) beneficial bacteria (Lachnospiraceae and Ruminoccocaceae) and decreasing (p < 0.05) harmful bacteria (Clostridiaceae, Enterobacteriaceae, and Helicobacteriacea), attenuating the effects promoted by the HFD ingestion. Only live cells could increase (p < 0.05) the HDL-cholesterol, while only inactivated cells caused attenuation (p < 0.05) of the blood pressure. Results show beneficial effects of live and inactivated L. casei 01 and indicate that ultrasound inactivation produces a paraprobiotic with similar or improved health properties compared to live cells.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundacao de Apoio a Pesquisa do Estado da Paraiba (FAPESQ)Univ Fed Paraiba, Dept Food Engn, Technol, Joao Pessoa, Paraiba, BrazilUniv Fed Paraiba, Hlth Sci Ctr, Dept Nutr, Joao Pessoa, Paraiba, BrazilFed Inst Sci & Technol Rio De Janeiro, Dept Food, Rio De Janeiro, RJ, BrazilUniv Fed Paraiba, Dept Biotechnol, Biotechnol Ctr, Joao Pessoa, Paraiba, BrazilUniv Claude Bernard Lyon 1, Univ Lyon, CarMeN Cardio Metab Diabet & Nutr Lab, INSERM,INRAE,INSA Lyon, Oullins, FranceUniv Illinois, Res Resource Ctr, Res Informat Core, Chicago, IL USASao Paulo State Univ, Dept Gen & Appl Biol, Inst Biosci, Rio Claro, SP, BrazilFed Inst Parana, Campus Paranavai, Paranavai, BrazilSao Paulo State Univ, Dept Gen & Appl Biol, Inst Biosci, Rio Claro, SP, BrazilCNPq: 303437/2017-0CNPq: 405644/2018-3CAPES: 001CAPES: UFPB PrInt 88881.311776/2018-01Fundacao de Apoio a Pesquisa do Estado da Paraiba (FAPESQ): 007/2019 FAPESQ-PB-MCT/CNPqRoyal Soc ChemistryUniv Fed ParaibaFed Inst Sci & Technol Rio De JaneiroUniv Claude Bernard Lyon 1Univ IllinoisUniversidade Estadual Paulista (Unesp)Fed Inst ParanaBrandao, Larissa RamalhoBrito Alves, Jose Luiz deCosta, Whyara Karoline Almeida daFerreira, Georgianna de Araujo HenriquesOliveira, Matthaws Pereira deGomes da Cruz, AdrianoBraga, Valdir de AndradeAquino, Jailane de SouzaVidal, HubertNoronha, Melline FontesCabral, Lucelia [UNESP]Pimentel, Tatiana ColomboMagnani, Marciane2021-06-25T15:05:33Z2021-06-25T15:05:33Z2021-05-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14http://dx.doi.org/10.1039/d1fo01064fFood & Function. Cambridge: Royal Soc Chemistry, 14 p., 2021.2042-6496http://hdl.handle.net/11449/21034610.1039/d1fo01064fWOS:000651904500001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFood & Functioninfo:eu-repo/semantics/openAccess2021-10-23T20:17:27Zoai:repositorio.unesp.br:11449/210346Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:33:24.423786Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Live and ultrasound-inactivated Lacticaseibacillus casei modulate the intestinal microbiota and improve biochemical and cardiovascular parameters in male rats fed a high-fat diet
title Live and ultrasound-inactivated Lacticaseibacillus casei modulate the intestinal microbiota and improve biochemical and cardiovascular parameters in male rats fed a high-fat diet
spellingShingle Live and ultrasound-inactivated Lacticaseibacillus casei modulate the intestinal microbiota and improve biochemical and cardiovascular parameters in male rats fed a high-fat diet
Brandao, Larissa Ramalho
title_short Live and ultrasound-inactivated Lacticaseibacillus casei modulate the intestinal microbiota and improve biochemical and cardiovascular parameters in male rats fed a high-fat diet
title_full Live and ultrasound-inactivated Lacticaseibacillus casei modulate the intestinal microbiota and improve biochemical and cardiovascular parameters in male rats fed a high-fat diet
title_fullStr Live and ultrasound-inactivated Lacticaseibacillus casei modulate the intestinal microbiota and improve biochemical and cardiovascular parameters in male rats fed a high-fat diet
title_full_unstemmed Live and ultrasound-inactivated Lacticaseibacillus casei modulate the intestinal microbiota and improve biochemical and cardiovascular parameters in male rats fed a high-fat diet
title_sort Live and ultrasound-inactivated Lacticaseibacillus casei modulate the intestinal microbiota and improve biochemical and cardiovascular parameters in male rats fed a high-fat diet
author Brandao, Larissa Ramalho
author_facet Brandao, Larissa Ramalho
Brito Alves, Jose Luiz de
Costa, Whyara Karoline Almeida da
Ferreira, Georgianna de Araujo Henriques
Oliveira, Matthaws Pereira de
Gomes da Cruz, Adriano
Braga, Valdir de Andrade
Aquino, Jailane de Souza
Vidal, Hubert
Noronha, Melline Fontes
Cabral, Lucelia [UNESP]
Pimentel, Tatiana Colombo
Magnani, Marciane
author_role author
author2 Brito Alves, Jose Luiz de
Costa, Whyara Karoline Almeida da
Ferreira, Georgianna de Araujo Henriques
Oliveira, Matthaws Pereira de
Gomes da Cruz, Adriano
Braga, Valdir de Andrade
Aquino, Jailane de Souza
Vidal, Hubert
Noronha, Melline Fontes
Cabral, Lucelia [UNESP]
Pimentel, Tatiana Colombo
Magnani, Marciane
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Fed Paraiba
Fed Inst Sci & Technol Rio De Janeiro
Univ Claude Bernard Lyon 1
Univ Illinois
Universidade Estadual Paulista (Unesp)
Fed Inst Parana
dc.contributor.author.fl_str_mv Brandao, Larissa Ramalho
Brito Alves, Jose Luiz de
Costa, Whyara Karoline Almeida da
Ferreira, Georgianna de Araujo Henriques
Oliveira, Matthaws Pereira de
Gomes da Cruz, Adriano
Braga, Valdir de Andrade
Aquino, Jailane de Souza
Vidal, Hubert
Noronha, Melline Fontes
Cabral, Lucelia [UNESP]
Pimentel, Tatiana Colombo
Magnani, Marciane
description This study aimed to evaluate the effects of ingestion of live (9 log CFU mL(-1)) and ultrasound-inactivated (paraprobiotic, 20 kHz, 40 min) Lacticaseibacillus casei 01 cells for 28 days on healthy parameters (biochemical and cardiovascular) and intestinal microbiota (amplicon sequencing of 16S ribosomal RNA) of rats fed a high-fat diet. Twenty-four male Wistar rats were divided into four groups of six animals: CTL (standard diet), HFD (high-fat diet), HFD-LC (high-fat diet and live L. casei), and HFD-ILC (high-fat diet and inactivated L. casei). The administration of live and ultrasound-inactivated L. casei prevented the increase (p < 0.05) in cholesterol levels (total and LDL) and controlled the insulin resistance in rats fed a high-fat diet. Furthermore, it promoted a modulation of the intestinal microbial composition by increasing (p < 0.05) beneficial bacteria (Lachnospiraceae and Ruminoccocaceae) and decreasing (p < 0.05) harmful bacteria (Clostridiaceae, Enterobacteriaceae, and Helicobacteriacea), attenuating the effects promoted by the HFD ingestion. Only live cells could increase (p < 0.05) the HDL-cholesterol, while only inactivated cells caused attenuation (p < 0.05) of the blood pressure. Results show beneficial effects of live and inactivated L. casei 01 and indicate that ultrasound inactivation produces a paraprobiotic with similar or improved health properties compared to live cells.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T15:05:33Z
2021-06-25T15:05:33Z
2021-05-08
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1039/d1fo01064f
Food & Function. Cambridge: Royal Soc Chemistry, 14 p., 2021.
2042-6496
http://hdl.handle.net/11449/210346
10.1039/d1fo01064f
WOS:000651904500001
url http://dx.doi.org/10.1039/d1fo01064f
http://hdl.handle.net/11449/210346
identifier_str_mv Food & Function. Cambridge: Royal Soc Chemistry, 14 p., 2021.
2042-6496
10.1039/d1fo01064f
WOS:000651904500001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Food & Function
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14
dc.publisher.none.fl_str_mv Royal Soc Chemistry
publisher.none.fl_str_mv Royal Soc Chemistry
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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