P-Glycoprotein and Androgen Receptor Expression Reveals Independence of Canine Prostate Cancer from Androgen Hormone Stimulation
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijms23031163 http://hdl.handle.net/11449/230240 |
Resumo: | Canine prostate cancer (PC) is an aggressive disease, and dogs can be considered comparative models for human PC. In recent years, canine PC has been shown to resemble human castrate-resistant prostate cancer. The influx and efflux of testosterone in prostatic luminal cells are regulated by P-glycoprotein (P-gp). Therefore, human PC generally lacks P-gp expression and maintains the expression of androgen receptors (ARs). However, this co-expression has not previ-ously been investigated in dogs. Therefore, this study aimed to evaluate AR and P-gp co-expression to elucidate these protein patterns in canine prostate samples. We identified AR/P-gp double im-munofluorescence co-expression of both proteins in normal luminal cells. However, in canine PC, cells lack AR expression and exhibit increased P-gp expression. These results were confirmed by gene expression analyses. Overall, our results strongly suggest that normal canine prostate testosterone influx may be regulated by P-gp expression, and that during progression to PC, prostatic cells lack AR expression and P-gp overexpress. P-gp expression in canine PC may be related to a phenotype of multiple drug resistance. |
id |
UNSP_c055ab2a7bab2db26afa434f3d0c70dd |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/230240 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
P-Glycoprotein and Androgen Receptor Expression Reveals Independence of Canine Prostate Cancer from Androgen Hormone StimulationABCB1Comparative oncologyProstatic diseaseTestosteroneCanine prostate cancer (PC) is an aggressive disease, and dogs can be considered comparative models for human PC. In recent years, canine PC has been shown to resemble human castrate-resistant prostate cancer. The influx and efflux of testosterone in prostatic luminal cells are regulated by P-glycoprotein (P-gp). Therefore, human PC generally lacks P-gp expression and maintains the expression of androgen receptors (ARs). However, this co-expression has not previ-ously been investigated in dogs. Therefore, this study aimed to evaluate AR and P-gp co-expression to elucidate these protein patterns in canine prostate samples. We identified AR/P-gp double im-munofluorescence co-expression of both proteins in normal luminal cells. However, in canine PC, cells lack AR expression and exhibit increased P-gp expression. These results were confirmed by gene expression analyses. Overall, our results strongly suggest that normal canine prostate testosterone influx may be regulated by P-gp expression, and that during progression to PC, prostatic cells lack AR expression and P-gp overexpress. P-gp expression in canine PC may be related to a phenotype of multiple drug resistance.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University—UNESPDepartment of Electrical Engineering School of Electrical Mechanical and Computer Engineering Federal University of Goias—UFGDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University—UNESPInstitute of Health Sciences Paulista University—UNIPDepartment of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University—UNESPDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University—UNESPFAPESP: 2015/25400-7Universidade Estadual Paulista (UNESP)Federal University of Goias—UFGPaulista University—UNIPCavalca, Alexandre Matheus Baesso [UNESP]Brandi, Andressa [UNESP]Fonseca-Alves, Ricardo HenriqueLaufer-Amorim, Renée [UNESP]Fonseca-Alves, Carlos Eduardo [UNESP]2022-04-29T08:38:41Z2022-04-29T08:38:41Z2022-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ijms23031163International Journal of Molecular Sciences, v. 23, n. 3, 2022.1422-00671661-6596http://hdl.handle.net/11449/23024010.3390/ijms230311632-s2.0-85123018176Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciencesinfo:eu-repo/semantics/openAccess2022-04-29T08:38:41Zoai:repositorio.unesp.br:11449/230240Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:02:30.981210Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
P-Glycoprotein and Androgen Receptor Expression Reveals Independence of Canine Prostate Cancer from Androgen Hormone Stimulation |
title |
P-Glycoprotein and Androgen Receptor Expression Reveals Independence of Canine Prostate Cancer from Androgen Hormone Stimulation |
spellingShingle |
P-Glycoprotein and Androgen Receptor Expression Reveals Independence of Canine Prostate Cancer from Androgen Hormone Stimulation Cavalca, Alexandre Matheus Baesso [UNESP] ABCB1 Comparative oncology Prostatic disease Testosterone |
title_short |
P-Glycoprotein and Androgen Receptor Expression Reveals Independence of Canine Prostate Cancer from Androgen Hormone Stimulation |
title_full |
P-Glycoprotein and Androgen Receptor Expression Reveals Independence of Canine Prostate Cancer from Androgen Hormone Stimulation |
title_fullStr |
P-Glycoprotein and Androgen Receptor Expression Reveals Independence of Canine Prostate Cancer from Androgen Hormone Stimulation |
title_full_unstemmed |
P-Glycoprotein and Androgen Receptor Expression Reveals Independence of Canine Prostate Cancer from Androgen Hormone Stimulation |
title_sort |
P-Glycoprotein and Androgen Receptor Expression Reveals Independence of Canine Prostate Cancer from Androgen Hormone Stimulation |
author |
Cavalca, Alexandre Matheus Baesso [UNESP] |
author_facet |
Cavalca, Alexandre Matheus Baesso [UNESP] Brandi, Andressa [UNESP] Fonseca-Alves, Ricardo Henrique Laufer-Amorim, Renée [UNESP] Fonseca-Alves, Carlos Eduardo [UNESP] |
author_role |
author |
author2 |
Brandi, Andressa [UNESP] Fonseca-Alves, Ricardo Henrique Laufer-Amorim, Renée [UNESP] Fonseca-Alves, Carlos Eduardo [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Federal University of Goias—UFG Paulista University—UNIP |
dc.contributor.author.fl_str_mv |
Cavalca, Alexandre Matheus Baesso [UNESP] Brandi, Andressa [UNESP] Fonseca-Alves, Ricardo Henrique Laufer-Amorim, Renée [UNESP] Fonseca-Alves, Carlos Eduardo [UNESP] |
dc.subject.por.fl_str_mv |
ABCB1 Comparative oncology Prostatic disease Testosterone |
topic |
ABCB1 Comparative oncology Prostatic disease Testosterone |
description |
Canine prostate cancer (PC) is an aggressive disease, and dogs can be considered comparative models for human PC. In recent years, canine PC has been shown to resemble human castrate-resistant prostate cancer. The influx and efflux of testosterone in prostatic luminal cells are regulated by P-glycoprotein (P-gp). Therefore, human PC generally lacks P-gp expression and maintains the expression of androgen receptors (ARs). However, this co-expression has not previ-ously been investigated in dogs. Therefore, this study aimed to evaluate AR and P-gp co-expression to elucidate these protein patterns in canine prostate samples. We identified AR/P-gp double im-munofluorescence co-expression of both proteins in normal luminal cells. However, in canine PC, cells lack AR expression and exhibit increased P-gp expression. These results were confirmed by gene expression analyses. Overall, our results strongly suggest that normal canine prostate testosterone influx may be regulated by P-gp expression, and that during progression to PC, prostatic cells lack AR expression and P-gp overexpress. P-gp expression in canine PC may be related to a phenotype of multiple drug resistance. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:38:41Z 2022-04-29T08:38:41Z 2022-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijms23031163 International Journal of Molecular Sciences, v. 23, n. 3, 2022. 1422-0067 1661-6596 http://hdl.handle.net/11449/230240 10.3390/ijms23031163 2-s2.0-85123018176 |
url |
http://dx.doi.org/10.3390/ijms23031163 http://hdl.handle.net/11449/230240 |
identifier_str_mv |
International Journal of Molecular Sciences, v. 23, n. 3, 2022. 1422-0067 1661-6596 10.3390/ijms23031163 2-s2.0-85123018176 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Molecular Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129276649668608 |