Asymmetric ketone reduction by immobilized Rhodotorula mucilaginosa
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/catal8040165 http://hdl.handle.net/11449/171007 |
Resumo: | In our previous study, Rhodotorula mucilaginosa (R. mucilaginosa) was selected via high throughput screening as a very active and selective whole-cell biocatalyst for the asymmetric reduction of ketones. In this study, the reduction of ketones to the desired chiral alcohols by immobilized cells of this strain was investigated. Characterization with Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) showed that whole R. mucilaginosa cells were successfully immobilized on support matrices composed of agar, calcium alginate, PVA-alginate and chitosan. The immobilized cells were applied to the enantioselective reduction of fourteen different aromatic ketones. Good to excellent results were achieved with R. mucilaginosa cells immobilized on agar and calcium alginate. The immobilized cells on the selected support matrix composed of agar exhibited a significant increase in pH tolerance at pH 3.5-9 and demonstrated highly improved thermal stability compared to free cells. The cells immobilized on agar retained 90% activity after 60 days storage at 4 ° C and retained almost 100% activity after 6 reuse cycles. In addition, the immobilization procedures are very simple and cause minimal pollution. These results suggest that the application of immobilized R. mucilaginosa can be practical on an industrial scale to produce chiral alcohols. |
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Asymmetric ketone reduction by immobilized Rhodotorula mucilaginosaAsymmetric reductionBiocatalystImmobilizationRhodotorula mucilaginosaStabilityIn our previous study, Rhodotorula mucilaginosa (R. mucilaginosa) was selected via high throughput screening as a very active and selective whole-cell biocatalyst for the asymmetric reduction of ketones. In this study, the reduction of ketones to the desired chiral alcohols by immobilized cells of this strain was investigated. Characterization with Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) showed that whole R. mucilaginosa cells were successfully immobilized on support matrices composed of agar, calcium alginate, PVA-alginate and chitosan. The immobilized cells were applied to the enantioselective reduction of fourteen different aromatic ketones. Good to excellent results were achieved with R. mucilaginosa cells immobilized on agar and calcium alginate. The immobilized cells on the selected support matrix composed of agar exhibited a significant increase in pH tolerance at pH 3.5-9 and demonstrated highly improved thermal stability compared to free cells. The cells immobilized on agar retained 90% activity after 60 days storage at 4 ° C and retained almost 100% activity after 6 reuse cycles. In addition, the immobilization procedures are very simple and cause minimal pollution. These results suggest that the application of immobilized R. mucilaginosa can be practical on an industrial scale to produce chiral alcohols.Sun Yat-sen UniversityNational Natural Science Foundation of ChinaSchool of Marine Sciences Sun Yat-Sen UniversityShanghai Institute of Materia Medica Chinese Academy of SciencesUniversity of Chinese Academy of SciencesDepartamento de Química Faculdade de Ciências UNESPDepartamento de Química Faculdade de Ciências UNESPSun Yat-sen University: 17lgpy58National Natural Science Foundation of China: 41706148Sun Yat-Sen UniversityChinese Academy of SciencesUniversity of Chinese Academy of SciencesUniversidade Estadual Paulista (Unesp)Liu, HuiDuan, Wen-Dide Souza, Fayene Zeferino Ribeiro [UNESP]Liu, LanChen, Bi-Shuang2018-12-11T16:53:19Z2018-12-11T16:53:19Z2018-04-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.3390/catal8040165Catalysts, v. 8, n. 4, 2018.2073-4344http://hdl.handle.net/11449/17100710.3390/catal80401652-s2.0-850471484572-s2.0-85047148457.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCatalysts0,855info:eu-repo/semantics/openAccess2023-12-26T06:15:43Zoai:repositorio.unesp.br:11449/171007Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:19:19.727532Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Asymmetric ketone reduction by immobilized Rhodotorula mucilaginosa |
title |
Asymmetric ketone reduction by immobilized Rhodotorula mucilaginosa |
spellingShingle |
Asymmetric ketone reduction by immobilized Rhodotorula mucilaginosa Liu, Hui Asymmetric reduction Biocatalyst Immobilization Rhodotorula mucilaginosa Stability |
title_short |
Asymmetric ketone reduction by immobilized Rhodotorula mucilaginosa |
title_full |
Asymmetric ketone reduction by immobilized Rhodotorula mucilaginosa |
title_fullStr |
Asymmetric ketone reduction by immobilized Rhodotorula mucilaginosa |
title_full_unstemmed |
Asymmetric ketone reduction by immobilized Rhodotorula mucilaginosa |
title_sort |
Asymmetric ketone reduction by immobilized Rhodotorula mucilaginosa |
author |
Liu, Hui |
author_facet |
Liu, Hui Duan, Wen-Di de Souza, Fayene Zeferino Ribeiro [UNESP] Liu, Lan Chen, Bi-Shuang |
author_role |
author |
author2 |
Duan, Wen-Di de Souza, Fayene Zeferino Ribeiro [UNESP] Liu, Lan Chen, Bi-Shuang |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Sun Yat-Sen University Chinese Academy of Sciences University of Chinese Academy of Sciences Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Liu, Hui Duan, Wen-Di de Souza, Fayene Zeferino Ribeiro [UNESP] Liu, Lan Chen, Bi-Shuang |
dc.subject.por.fl_str_mv |
Asymmetric reduction Biocatalyst Immobilization Rhodotorula mucilaginosa Stability |
topic |
Asymmetric reduction Biocatalyst Immobilization Rhodotorula mucilaginosa Stability |
description |
In our previous study, Rhodotorula mucilaginosa (R. mucilaginosa) was selected via high throughput screening as a very active and selective whole-cell biocatalyst for the asymmetric reduction of ketones. In this study, the reduction of ketones to the desired chiral alcohols by immobilized cells of this strain was investigated. Characterization with Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) showed that whole R. mucilaginosa cells were successfully immobilized on support matrices composed of agar, calcium alginate, PVA-alginate and chitosan. The immobilized cells were applied to the enantioselective reduction of fourteen different aromatic ketones. Good to excellent results were achieved with R. mucilaginosa cells immobilized on agar and calcium alginate. The immobilized cells on the selected support matrix composed of agar exhibited a significant increase in pH tolerance at pH 3.5-9 and demonstrated highly improved thermal stability compared to free cells. The cells immobilized on agar retained 90% activity after 60 days storage at 4 ° C and retained almost 100% activity after 6 reuse cycles. In addition, the immobilization procedures are very simple and cause minimal pollution. These results suggest that the application of immobilized R. mucilaginosa can be practical on an industrial scale to produce chiral alcohols. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:53:19Z 2018-12-11T16:53:19Z 2018-04-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/catal8040165 Catalysts, v. 8, n. 4, 2018. 2073-4344 http://hdl.handle.net/11449/171007 10.3390/catal8040165 2-s2.0-85047148457 2-s2.0-85047148457.pdf |
url |
http://dx.doi.org/10.3390/catal8040165 http://hdl.handle.net/11449/171007 |
identifier_str_mv |
Catalysts, v. 8, n. 4, 2018. 2073-4344 10.3390/catal8040165 2-s2.0-85047148457 2-s2.0-85047148457.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Catalysts 0,855 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129308892332032 |