Assessing genetic diversity for a pre-breeding program in piaractus mesopotamicus by snps and ssrs
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/genes10090668 http://hdl.handle.net/11449/199426 |
Resumo: | The pacu (Piaractus mesopotamicus) is a Neotropical fish with remarkable productive performance for aquaculture. Knowledge of genetic resources in Neotropical fish is essential for their applications in breeding programs. The aim of this study was to characterize the genetic diversity of seven farmed populations of pacu which will constitute the basis for a broodstock foundation for coming breeding programs in Brazil. Analysis of one wild population (Paraná River) was used as a reference to compare genetic parameters in the farmed populations. The analyses were performed using 32 single-nucleotide polymorphisms (SNP) and 8 simple sequence repeat (SSR) markers. No significant differences in genetic diversity between populations estimated through the number of alleles and allelic richness, observed heterozygosity, expected heterozygosity, and minimum allele frequency were detected (p > 0.05). Low genetic diversity was observed in all farmed stocks and the wild population. Moreover, we detected low genetic structure when comparing farmed and wild populations for SNPs (FST = 0.07; K = 3) and SSRs (FST = 0.08; K = 2). Analysis of molecular variance (AMOVA) demonstrated that genetic variation was mostly within populations. Kinship analysis showed that most fish farms included related individuals at a proportion of at least 25%. Our results suggest that the basal broodstock for pacu breeding programs should be founded with individuals from different fish farms for higher genetic diversity and to avoid inbreeding risks. |
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Assessing genetic diversity for a pre-breeding program in piaractus mesopotamicus by snps and ssrsAquacultureBreeding programsGenetic variabilityInbreedingThe pacu (Piaractus mesopotamicus) is a Neotropical fish with remarkable productive performance for aquaculture. Knowledge of genetic resources in Neotropical fish is essential for their applications in breeding programs. The aim of this study was to characterize the genetic diversity of seven farmed populations of pacu which will constitute the basis for a broodstock foundation for coming breeding programs in Brazil. Analysis of one wild population (Paraná River) was used as a reference to compare genetic parameters in the farmed populations. The analyses were performed using 32 single-nucleotide polymorphisms (SNP) and 8 simple sequence repeat (SSR) markers. No significant differences in genetic diversity between populations estimated through the number of alleles and allelic richness, observed heterozygosity, expected heterozygosity, and minimum allele frequency were detected (p > 0.05). Low genetic diversity was observed in all farmed stocks and the wild population. Moreover, we detected low genetic structure when comparing farmed and wild populations for SNPs (FST = 0.07; K = 3) and SSRs (FST = 0.08; K = 2). Analysis of molecular variance (AMOVA) demonstrated that genetic variation was mostly within populations. Kinship analysis showed that most fish farms included related individuals at a proportion of at least 25%. Our results suggest that the basal broodstock for pacu breeding programs should be founded with individuals from different fish farms for higher genetic diversity and to avoid inbreeding risks.Aquaculture Center of Unesp São Paulo State University (Unesp)Laboratorio Mixto de Biotecnología Acuática - Universidad Nacional de Rosario Facultad de Ciencias Bioquímicas y Farmacéuticas - Ministerio de Ciencia Tecnología e Innovación productiva de Santa Fe.Centro Científico y Tecnológico Acuario del Río ParanáConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Institute of Biosciences São Paulo State University (Unesp)Facultad de Veterinaria Universidad de Santiago de Compostela (USC)Instituto de Acuicultura Universidad de Santiago de Compostela (USC)São Paulo State University (Unesp) School of SciencesAquaculture Center of Unesp São Paulo State University (Unesp)Institute of Biosciences São Paulo State University (Unesp)São Paulo State University (Unesp) School of SciencesUniversidade Estadual Paulista (Unesp)Tecnología e Innovación productiva de Santa Fe.Centro Científico y Tecnológico Acuario del Río ParanáConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Universidad de Santiago de Compostela (USC)Mastrochirico-Filho, Vito Antonio [UNESP]Pazo, Felipe DelHata, Milene Elissa [UNESP]Villanova, Gabriela VaninaForesti, Fausto [UNESP]Vera, ManuelMartínez, PaulinoPorto-Foresti, Fábio [UNESP]Hashimoto, Diogo Teruo [UNESP]2020-12-12T01:39:26Z2020-12-12T01:39:26Z2019-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/genes10090668Genes, v. 10, n. 9, 2019.2073-4425http://hdl.handle.net/11449/19942610.3390/genes100906682-s2.0-85072223894Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenesinfo:eu-repo/semantics/openAccess2024-04-09T15:43:44Zoai:repositorio.unesp.br:11449/199426Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:43:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Assessing genetic diversity for a pre-breeding program in piaractus mesopotamicus by snps and ssrs |
title |
Assessing genetic diversity for a pre-breeding program in piaractus mesopotamicus by snps and ssrs |
spellingShingle |
Assessing genetic diversity for a pre-breeding program in piaractus mesopotamicus by snps and ssrs Mastrochirico-Filho, Vito Antonio [UNESP] Aquaculture Breeding programs Genetic variability Inbreeding |
title_short |
Assessing genetic diversity for a pre-breeding program in piaractus mesopotamicus by snps and ssrs |
title_full |
Assessing genetic diversity for a pre-breeding program in piaractus mesopotamicus by snps and ssrs |
title_fullStr |
Assessing genetic diversity for a pre-breeding program in piaractus mesopotamicus by snps and ssrs |
title_full_unstemmed |
Assessing genetic diversity for a pre-breeding program in piaractus mesopotamicus by snps and ssrs |
title_sort |
Assessing genetic diversity for a pre-breeding program in piaractus mesopotamicus by snps and ssrs |
author |
Mastrochirico-Filho, Vito Antonio [UNESP] |
author_facet |
Mastrochirico-Filho, Vito Antonio [UNESP] Pazo, Felipe Del Hata, Milene Elissa [UNESP] Villanova, Gabriela Vanina Foresti, Fausto [UNESP] Vera, Manuel Martínez, Paulino Porto-Foresti, Fábio [UNESP] Hashimoto, Diogo Teruo [UNESP] |
author_role |
author |
author2 |
Pazo, Felipe Del Hata, Milene Elissa [UNESP] Villanova, Gabriela Vanina Foresti, Fausto [UNESP] Vera, Manuel Martínez, Paulino Porto-Foresti, Fábio [UNESP] Hashimoto, Diogo Teruo [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Tecnología e Innovación productiva de Santa Fe.Centro Científico y Tecnológico Acuario del Río Paraná Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) Universidad de Santiago de Compostela (USC) |
dc.contributor.author.fl_str_mv |
Mastrochirico-Filho, Vito Antonio [UNESP] Pazo, Felipe Del Hata, Milene Elissa [UNESP] Villanova, Gabriela Vanina Foresti, Fausto [UNESP] Vera, Manuel Martínez, Paulino Porto-Foresti, Fábio [UNESP] Hashimoto, Diogo Teruo [UNESP] |
dc.subject.por.fl_str_mv |
Aquaculture Breeding programs Genetic variability Inbreeding |
topic |
Aquaculture Breeding programs Genetic variability Inbreeding |
description |
The pacu (Piaractus mesopotamicus) is a Neotropical fish with remarkable productive performance for aquaculture. Knowledge of genetic resources in Neotropical fish is essential for their applications in breeding programs. The aim of this study was to characterize the genetic diversity of seven farmed populations of pacu which will constitute the basis for a broodstock foundation for coming breeding programs in Brazil. Analysis of one wild population (Paraná River) was used as a reference to compare genetic parameters in the farmed populations. The analyses were performed using 32 single-nucleotide polymorphisms (SNP) and 8 simple sequence repeat (SSR) markers. No significant differences in genetic diversity between populations estimated through the number of alleles and allelic richness, observed heterozygosity, expected heterozygosity, and minimum allele frequency were detected (p > 0.05). Low genetic diversity was observed in all farmed stocks and the wild population. Moreover, we detected low genetic structure when comparing farmed and wild populations for SNPs (FST = 0.07; K = 3) and SSRs (FST = 0.08; K = 2). Analysis of molecular variance (AMOVA) demonstrated that genetic variation was mostly within populations. Kinship analysis showed that most fish farms included related individuals at a proportion of at least 25%. Our results suggest that the basal broodstock for pacu breeding programs should be founded with individuals from different fish farms for higher genetic diversity and to avoid inbreeding risks. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-01 2020-12-12T01:39:26Z 2020-12-12T01:39:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/genes10090668 Genes, v. 10, n. 9, 2019. 2073-4425 http://hdl.handle.net/11449/199426 10.3390/genes10090668 2-s2.0-85072223894 |
url |
http://dx.doi.org/10.3390/genes10090668 http://hdl.handle.net/11449/199426 |
identifier_str_mv |
Genes, v. 10, n. 9, 2019. 2073-4425 10.3390/genes10090668 2-s2.0-85072223894 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genes |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803650363091320832 |