Propolis reduces Leishmania amazonensis-induced inflammation in the liver of BALB/c mice
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00436-015-4890-4 http://hdl.handle.net/11449/172359 |
Resumo: | Experimental models of mouse paw infection with L. amazonensis show an induction of a strong inflammatory response in the skin, and parasitic migration may occur to secondary organs with consequent tissue injury. There are few studies focusing on the resolution of damage in secondary organs caused by Leishmania species-related cutaneous leishmaniasis. We investigated the propolis treatment effect on liver inflammation induced by Leishmania amazonensis infection in the mouse paw. BALB/c mice were infected in the hind paw with L. amazonensis (107) promastigote forms. After 15 days, animals were treated daily with propolis (5 mg/kg), Glucantime (10 mg/kg), or with propolis plus Glucantime combined. After 60 days, mice were euthanized and livers were collected for inflammatory process analysis. Liver microscopic analysis showed that propolis reduced the inflammatory process compared to untreated infected control. There was a decrease of liver myeloperoxidase and N-acetyl-β-glucosaminidase activity levels, collagen fiber deposition, pro-inflammatory cytokine production, and plasma aspartate transaminase and alanine transaminase levels. Furthermore, propolis treatment enhanced anti-inflammatory cytokine levels and reversed hepatosplenomegaly. Our data demonstrated that daily low doses of Brazilian propolis reduced the secondary chronic inflammatory process in the liver caused by L. amazonensis subcutaneous infection in a susceptible mice strain. |
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Propolis reduces Leishmania amazonensis-induced inflammation in the liver of BALB/c miceGlucantimeInflammationLeishmania amazonensisLiverPropolisExperimental models of mouse paw infection with L. amazonensis show an induction of a strong inflammatory response in the skin, and parasitic migration may occur to secondary organs with consequent tissue injury. There are few studies focusing on the resolution of damage in secondary organs caused by Leishmania species-related cutaneous leishmaniasis. We investigated the propolis treatment effect on liver inflammation induced by Leishmania amazonensis infection in the mouse paw. BALB/c mice were infected in the hind paw with L. amazonensis (107) promastigote forms. After 15 days, animals were treated daily with propolis (5 mg/kg), Glucantime (10 mg/kg), or with propolis plus Glucantime combined. After 60 days, mice were euthanized and livers were collected for inflammatory process analysis. Liver microscopic analysis showed that propolis reduced the inflammatory process compared to untreated infected control. There was a decrease of liver myeloperoxidase and N-acetyl-β-glucosaminidase activity levels, collagen fiber deposition, pro-inflammatory cytokine production, and plasma aspartate transaminase and alanine transaminase levels. Furthermore, propolis treatment enhanced anti-inflammatory cytokine levels and reversed hepatosplenomegaly. Our data demonstrated that daily low doses of Brazilian propolis reduced the secondary chronic inflammatory process in the liver caused by L. amazonensis subcutaneous infection in a susceptible mice strain.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Departamento de Ciências Patológicas Centro de Ciências Biológicas Universidade Estadual de LondrinaDepartamento de Histologia Centro de Ciências Biológicas Universidade Estadual de LondrinaLaboratório de Mediadores Inflamatórios Universidade do Oeste do Paraná UNIOESTEDepartamento de Microbiologia e Imunologia Instituto de Biociências Universidade Estadual Paulista UNESPDepartamento de Microbiologia e Imunologia Instituto de Biociências Universidade Estadual Paulista UNESPUniversidade Estadual de Londrina (UEL)UNIOESTEUniversidade Estadual Paulista (Unesp)da Silva, Suelen S.Mizokami, Sandra S.Fanti, Jacqueline R.Miranda, Milena M.Kawakami, Natalia Y.Teixeira, Fernanda HumelAraújo, Eduardo J. A.Panis, CarolinaWatanabe, Maria A. E.Sforcin, José M. [UNESP]Pavanelli, Wander R.Verri, Waldiceu A.Felipe, IoniceConchon-Costa, Ivete2018-12-11T16:59:54Z2018-12-11T16:59:54Z2016-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1557-1566application/pdfhttp://dx.doi.org/10.1007/s00436-015-4890-4Parasitology Research, v. 115, n. 4, p. 1557-1566, 2016.1432-19550932-0113http://hdl.handle.net/11449/17235910.1007/s00436-015-4890-42-s2.0-849519655672-s2.0-84951965567.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology Research0,9910,991info:eu-repo/semantics/openAccess2023-10-02T06:08:39Zoai:repositorio.unesp.br:11449/172359Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:49:27.316188Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Propolis reduces Leishmania amazonensis-induced inflammation in the liver of BALB/c mice |
title |
Propolis reduces Leishmania amazonensis-induced inflammation in the liver of BALB/c mice |
spellingShingle |
Propolis reduces Leishmania amazonensis-induced inflammation in the liver of BALB/c mice da Silva, Suelen S. Glucantime Inflammation Leishmania amazonensis Liver Propolis |
title_short |
Propolis reduces Leishmania amazonensis-induced inflammation in the liver of BALB/c mice |
title_full |
Propolis reduces Leishmania amazonensis-induced inflammation in the liver of BALB/c mice |
title_fullStr |
Propolis reduces Leishmania amazonensis-induced inflammation in the liver of BALB/c mice |
title_full_unstemmed |
Propolis reduces Leishmania amazonensis-induced inflammation in the liver of BALB/c mice |
title_sort |
Propolis reduces Leishmania amazonensis-induced inflammation in the liver of BALB/c mice |
author |
da Silva, Suelen S. |
author_facet |
da Silva, Suelen S. Mizokami, Sandra S. Fanti, Jacqueline R. Miranda, Milena M. Kawakami, Natalia Y. Teixeira, Fernanda Humel Araújo, Eduardo J. A. Panis, Carolina Watanabe, Maria A. E. Sforcin, José M. [UNESP] Pavanelli, Wander R. Verri, Waldiceu A. Felipe, Ionice Conchon-Costa, Ivete |
author_role |
author |
author2 |
Mizokami, Sandra S. Fanti, Jacqueline R. Miranda, Milena M. Kawakami, Natalia Y. Teixeira, Fernanda Humel Araújo, Eduardo J. A. Panis, Carolina Watanabe, Maria A. E. Sforcin, José M. [UNESP] Pavanelli, Wander R. Verri, Waldiceu A. Felipe, Ionice Conchon-Costa, Ivete |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) UNIOESTE Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
da Silva, Suelen S. Mizokami, Sandra S. Fanti, Jacqueline R. Miranda, Milena M. Kawakami, Natalia Y. Teixeira, Fernanda Humel Araújo, Eduardo J. A. Panis, Carolina Watanabe, Maria A. E. Sforcin, José M. [UNESP] Pavanelli, Wander R. Verri, Waldiceu A. Felipe, Ionice Conchon-Costa, Ivete |
dc.subject.por.fl_str_mv |
Glucantime Inflammation Leishmania amazonensis Liver Propolis |
topic |
Glucantime Inflammation Leishmania amazonensis Liver Propolis |
description |
Experimental models of mouse paw infection with L. amazonensis show an induction of a strong inflammatory response in the skin, and parasitic migration may occur to secondary organs with consequent tissue injury. There are few studies focusing on the resolution of damage in secondary organs caused by Leishmania species-related cutaneous leishmaniasis. We investigated the propolis treatment effect on liver inflammation induced by Leishmania amazonensis infection in the mouse paw. BALB/c mice were infected in the hind paw with L. amazonensis (107) promastigote forms. After 15 days, animals were treated daily with propolis (5 mg/kg), Glucantime (10 mg/kg), or with propolis plus Glucantime combined. After 60 days, mice were euthanized and livers were collected for inflammatory process analysis. Liver microscopic analysis showed that propolis reduced the inflammatory process compared to untreated infected control. There was a decrease of liver myeloperoxidase and N-acetyl-β-glucosaminidase activity levels, collagen fiber deposition, pro-inflammatory cytokine production, and plasma aspartate transaminase and alanine transaminase levels. Furthermore, propolis treatment enhanced anti-inflammatory cytokine levels and reversed hepatosplenomegaly. Our data demonstrated that daily low doses of Brazilian propolis reduced the secondary chronic inflammatory process in the liver caused by L. amazonensis subcutaneous infection in a susceptible mice strain. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04-01 2018-12-11T16:59:54Z 2018-12-11T16:59:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00436-015-4890-4 Parasitology Research, v. 115, n. 4, p. 1557-1566, 2016. 1432-1955 0932-0113 http://hdl.handle.net/11449/172359 10.1007/s00436-015-4890-4 2-s2.0-84951965567 2-s2.0-84951965567.pdf |
url |
http://dx.doi.org/10.1007/s00436-015-4890-4 http://hdl.handle.net/11449/172359 |
identifier_str_mv |
Parasitology Research, v. 115, n. 4, p. 1557-1566, 2016. 1432-1955 0932-0113 10.1007/s00436-015-4890-4 2-s2.0-84951965567 2-s2.0-84951965567.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Parasitology Research 0,991 0,991 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1557-1566 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128279535681536 |