Overall-Mouth Disinfection by Photodynamic Therapy Using Curcumin
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1089/pho.2011.3053 http://hdl.handle.net/11449/7375 |
Resumo: | Background data: Photodynamic therapy is a technique that involves the activation of photosensitizers by light in the presence of tissue oxygen, resulting in the production of reactive radicals capable of inducing cell death. Objective: The present study assessed the overall susceptibility of pathogens of salivary flora to photodynamic therapy after sensitization with curcumin and exposure to blue light at 450 nm. Methods: A randomized trial was executed with 13 adult volunteers. Three different groups were analyzed: L-D- (no light, no drug; control group), L-D+ (treated only with the drug; curcumin group) and L+D+ (treated with drug and light; photodynamic therapy group). Non-stimulated saliva samples were collected for bacterial counts at baseline and after the experimental phase, and adverse events experienced were recorded. Serial dilutions were performed, and the resulting samples were cultured on blood agar plates in microaerophilic conditions. The number of colony-forming units was then determined. Results: There was a considerable difference between the two experimental groups with regard to effectiveness of bacterial reduction. In the L-D+ group, the bacterial decline was considerably smaller (9%) than in the L+D+ group, with a 68% decrease in bacteria. A statistically significant reduction in the bacterial population was observed only in the photodynamic therapy group (p < 0.05). Conclusions: Photodynamic therapy was found to be effective in the reduction of salivary microorganisms. No significant reduction was found for the group in which only curcumin was used, proving the absence of dark toxicity of the drug. This work shows that overall disinfection of the mouth can be done with a simple procedure involving photodynamic action. |
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Repositório Institucional da UNESP |
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spelling |
Overall-Mouth Disinfection by Photodynamic Therapy Using CurcuminBackground data: Photodynamic therapy is a technique that involves the activation of photosensitizers by light in the presence of tissue oxygen, resulting in the production of reactive radicals capable of inducing cell death. Objective: The present study assessed the overall susceptibility of pathogens of salivary flora to photodynamic therapy after sensitization with curcumin and exposure to blue light at 450 nm. Methods: A randomized trial was executed with 13 adult volunteers. Three different groups were analyzed: L-D- (no light, no drug; control group), L-D+ (treated only with the drug; curcumin group) and L+D+ (treated with drug and light; photodynamic therapy group). Non-stimulated saliva samples were collected for bacterial counts at baseline and after the experimental phase, and adverse events experienced were recorded. Serial dilutions were performed, and the resulting samples were cultured on blood agar plates in microaerophilic conditions. The number of colony-forming units was then determined. Results: There was a considerable difference between the two experimental groups with regard to effectiveness of bacterial reduction. In the L-D+ group, the bacterial decline was considerably smaller (9%) than in the L+D+ group, with a 68% decrease in bacteria. A statistically significant reduction in the bacterial population was observed only in the photodynamic therapy group (p < 0.05). Conclusions: Photodynamic therapy was found to be effective in the reduction of salivary microorganisms. No significant reduction was found for the group in which only curcumin was used, proving the absence of dark toxicity of the drug. This work shows that overall disinfection of the mouth can be done with a simple procedure involving photodynamic action.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Financiadora de Estudos e Projetos (FINEP)Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE)Medical and Dental Equipment GnatusUniv Pernambuco, Dent Sch Pernambuco, Camaragibe, PE, BrazilUniv São Paulo State UNESP, Sch Pharmaceut Sci, Dept Clin Anal, Araraquara, SP, BrazilUniv Fed Sao Carlos, Inst Phys Sao Carlos, BR-13560 Sao Carlos, SP, BrazilUniv São Paulo State UNESP, Sch Pharmaceut Sci, Dept Clin Anal, Araraquara, SP, BrazilMary Ann Liebert, Inc.Univ PernambucoUniversidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Araujo, Natalia CostaFontana, Carla Raquel [UNESP]Martinez Gerbi, Marleny ElizabethBagnato, Vanderlei Salvador2014-05-20T13:24:02Z2014-05-20T13:24:02Z2012-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article96-101application/pdfhttp://dx.doi.org/10.1089/pho.2011.3053Photomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc., v. 30, n. 2, p. 96-101, 2012.1549-5418http://hdl.handle.net/11449/737510.1089/pho.2011.3053WOS:000300041100007WOS000300041100007.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotomedicine and Laser Surgery1.6200,443info:eu-repo/semantics/openAccess2024-06-21T15:18:45Zoai:repositorio.unesp.br:11449/7375Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:52:45.913797Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Overall-Mouth Disinfection by Photodynamic Therapy Using Curcumin |
title |
Overall-Mouth Disinfection by Photodynamic Therapy Using Curcumin |
spellingShingle |
Overall-Mouth Disinfection by Photodynamic Therapy Using Curcumin Araujo, Natalia Costa |
title_short |
Overall-Mouth Disinfection by Photodynamic Therapy Using Curcumin |
title_full |
Overall-Mouth Disinfection by Photodynamic Therapy Using Curcumin |
title_fullStr |
Overall-Mouth Disinfection by Photodynamic Therapy Using Curcumin |
title_full_unstemmed |
Overall-Mouth Disinfection by Photodynamic Therapy Using Curcumin |
title_sort |
Overall-Mouth Disinfection by Photodynamic Therapy Using Curcumin |
author |
Araujo, Natalia Costa |
author_facet |
Araujo, Natalia Costa Fontana, Carla Raquel [UNESP] Martinez Gerbi, Marleny Elizabeth Bagnato, Vanderlei Salvador |
author_role |
author |
author2 |
Fontana, Carla Raquel [UNESP] Martinez Gerbi, Marleny Elizabeth Bagnato, Vanderlei Salvador |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Univ Pernambuco Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Araujo, Natalia Costa Fontana, Carla Raquel [UNESP] Martinez Gerbi, Marleny Elizabeth Bagnato, Vanderlei Salvador |
description |
Background data: Photodynamic therapy is a technique that involves the activation of photosensitizers by light in the presence of tissue oxygen, resulting in the production of reactive radicals capable of inducing cell death. Objective: The present study assessed the overall susceptibility of pathogens of salivary flora to photodynamic therapy after sensitization with curcumin and exposure to blue light at 450 nm. Methods: A randomized trial was executed with 13 adult volunteers. Three different groups were analyzed: L-D- (no light, no drug; control group), L-D+ (treated only with the drug; curcumin group) and L+D+ (treated with drug and light; photodynamic therapy group). Non-stimulated saliva samples were collected for bacterial counts at baseline and after the experimental phase, and adverse events experienced were recorded. Serial dilutions were performed, and the resulting samples were cultured on blood agar plates in microaerophilic conditions. The number of colony-forming units was then determined. Results: There was a considerable difference between the two experimental groups with regard to effectiveness of bacterial reduction. In the L-D+ group, the bacterial decline was considerably smaller (9%) than in the L+D+ group, with a 68% decrease in bacteria. A statistically significant reduction in the bacterial population was observed only in the photodynamic therapy group (p < 0.05). Conclusions: Photodynamic therapy was found to be effective in the reduction of salivary microorganisms. No significant reduction was found for the group in which only curcumin was used, proving the absence of dark toxicity of the drug. This work shows that overall disinfection of the mouth can be done with a simple procedure involving photodynamic action. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-02-01 2014-05-20T13:24:02Z 2014-05-20T13:24:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1089/pho.2011.3053 Photomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc., v. 30, n. 2, p. 96-101, 2012. 1549-5418 http://hdl.handle.net/11449/7375 10.1089/pho.2011.3053 WOS:000300041100007 WOS000300041100007.pdf |
url |
http://dx.doi.org/10.1089/pho.2011.3053 http://hdl.handle.net/11449/7375 |
identifier_str_mv |
Photomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc., v. 30, n. 2, p. 96-101, 2012. 1549-5418 10.1089/pho.2011.3053 WOS:000300041100007 WOS000300041100007.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photomedicine and Laser Surgery 1.620 0,443 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
96-101 application/pdf |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert, Inc. |
publisher.none.fl_str_mv |
Mary Ann Liebert, Inc. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128714421043200 |