Evidence for post-zygotic self-incompatibility in Handroanthus impetiginosus (Bignoniaceae)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00497-017-0300-7 http://hdl.handle.net/11449/162891 |
Resumo: | Late-acting self-incompatibility (LSI) has been defined as a genetically controlled self-sterility mechanism that prevents seed set by selfing, despite normal pollen tube growth and ovule penetration in self-pollinated pistils. In species of the Bignoniaceae with LSI, such as Handroanthus impetiginosus, the selfed pistils are characterized by a marked delay in ovule penetration, fertilization, and endosperm initiation, followed by uniform pistil abscission. This highlights the contentious possibility of a post-zygotic self-incompatibility system. However, previous studies were unable to confirm fusion of the sperm and egg cell nuclei in selfed ovules. In the present study, the cytology of the embryo sac, double fertilization, and pistil longevity was investigated in H. impetiginosus using comparative nuclei microspectrofluorometry of DAPI-stained sections of self- vs. unpollinated pistils. Differences in both pistil longevity and ovary size between self- and unpollinated flowers at the time of pistil abscission were significant. Zygotes with double the DNA content in their nuclei relative to unfertilized egg cell nuclei were verified in selfed ovules from the first day after pollination onward, and G1 karyogamy appeared to have occurred. Our cytological analysis clearly indicates that ovules of self-pollinated pistils in H. impetiginosus are fertilized before pistil abscission but no embryogenesis initiation occurs, which strongly supports the idea of a post-zygotic self-incompatibility mechanism. |
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Evidence for post-zygotic self-incompatibility in Handroanthus impetiginosus (Bignoniaceae)Late-acting self-incompatibilitySelf-sterilityDNA microspectrofluorometryKaryogamyFertilizationFemale gametophyteBreeding systemLate-acting self-incompatibility (LSI) has been defined as a genetically controlled self-sterility mechanism that prevents seed set by selfing, despite normal pollen tube growth and ovule penetration in self-pollinated pistils. In species of the Bignoniaceae with LSI, such as Handroanthus impetiginosus, the selfed pistils are characterized by a marked delay in ovule penetration, fertilization, and endosperm initiation, followed by uniform pistil abscission. This highlights the contentious possibility of a post-zygotic self-incompatibility system. However, previous studies were unable to confirm fusion of the sperm and egg cell nuclei in selfed ovules. In the present study, the cytology of the embryo sac, double fertilization, and pistil longevity was investigated in H. impetiginosus using comparative nuclei microspectrofluorometry of DAPI-stained sections of self- vs. unpollinated pistils. Differences in both pistil longevity and ovary size between self- and unpollinated flowers at the time of pistil abscission were significant. Zygotes with double the DNA content in their nuclei relative to unfertilized egg cell nuclei were verified in selfed ovules from the first day after pollination onward, and G1 karyogamy appeared to have occurred. Our cytological analysis clearly indicates that ovules of self-pollinated pistils in H. impetiginosus are fertilized before pistil abscission but no embryogenesis initiation occurs, which strongly supports the idea of a post-zygotic self-incompatibility mechanism.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Zool & Bot, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Zool & Bot, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilFAPESP: 2005/59234-4SpringerUniversidade Estadual Paulista (Unesp)Bittencourt Junior, Nelson Sabino [UNESP]2018-11-26T17:34:50Z2018-11-26T17:34:50Z2017-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article69-79application/pdfhttp://dx.doi.org/10.1007/s00497-017-0300-7Plant Reproduction. New York: Springer, v. 30, n. 2, p. 69-79, 2017.2194-7953http://hdl.handle.net/11449/16289110.1007/s00497-017-0300-7WOS:000403546900001WOS000403546900001.pdf7010826495155959Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlant Reproduction1,597info:eu-repo/semantics/openAccess2023-12-18T06:21:13Zoai:repositorio.unesp.br:11449/162891Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:41:58.328974Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evidence for post-zygotic self-incompatibility in Handroanthus impetiginosus (Bignoniaceae) |
title |
Evidence for post-zygotic self-incompatibility in Handroanthus impetiginosus (Bignoniaceae) |
spellingShingle |
Evidence for post-zygotic self-incompatibility in Handroanthus impetiginosus (Bignoniaceae) Bittencourt Junior, Nelson Sabino [UNESP] Late-acting self-incompatibility Self-sterility DNA microspectrofluorometry Karyogamy Fertilization Female gametophyte Breeding system |
title_short |
Evidence for post-zygotic self-incompatibility in Handroanthus impetiginosus (Bignoniaceae) |
title_full |
Evidence for post-zygotic self-incompatibility in Handroanthus impetiginosus (Bignoniaceae) |
title_fullStr |
Evidence for post-zygotic self-incompatibility in Handroanthus impetiginosus (Bignoniaceae) |
title_full_unstemmed |
Evidence for post-zygotic self-incompatibility in Handroanthus impetiginosus (Bignoniaceae) |
title_sort |
Evidence for post-zygotic self-incompatibility in Handroanthus impetiginosus (Bignoniaceae) |
author |
Bittencourt Junior, Nelson Sabino [UNESP] |
author_facet |
Bittencourt Junior, Nelson Sabino [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bittencourt Junior, Nelson Sabino [UNESP] |
dc.subject.por.fl_str_mv |
Late-acting self-incompatibility Self-sterility DNA microspectrofluorometry Karyogamy Fertilization Female gametophyte Breeding system |
topic |
Late-acting self-incompatibility Self-sterility DNA microspectrofluorometry Karyogamy Fertilization Female gametophyte Breeding system |
description |
Late-acting self-incompatibility (LSI) has been defined as a genetically controlled self-sterility mechanism that prevents seed set by selfing, despite normal pollen tube growth and ovule penetration in self-pollinated pistils. In species of the Bignoniaceae with LSI, such as Handroanthus impetiginosus, the selfed pistils are characterized by a marked delay in ovule penetration, fertilization, and endosperm initiation, followed by uniform pistil abscission. This highlights the contentious possibility of a post-zygotic self-incompatibility system. However, previous studies were unable to confirm fusion of the sperm and egg cell nuclei in selfed ovules. In the present study, the cytology of the embryo sac, double fertilization, and pistil longevity was investigated in H. impetiginosus using comparative nuclei microspectrofluorometry of DAPI-stained sections of self- vs. unpollinated pistils. Differences in both pistil longevity and ovary size between self- and unpollinated flowers at the time of pistil abscission were significant. Zygotes with double the DNA content in their nuclei relative to unfertilized egg cell nuclei were verified in selfed ovules from the first day after pollination onward, and G1 karyogamy appeared to have occurred. Our cytological analysis clearly indicates that ovules of self-pollinated pistils in H. impetiginosus are fertilized before pistil abscission but no embryogenesis initiation occurs, which strongly supports the idea of a post-zygotic self-incompatibility mechanism. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 2018-11-26T17:34:50Z 2018-11-26T17:34:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00497-017-0300-7 Plant Reproduction. New York: Springer, v. 30, n. 2, p. 69-79, 2017. 2194-7953 http://hdl.handle.net/11449/162891 10.1007/s00497-017-0300-7 WOS:000403546900001 WOS000403546900001.pdf 7010826495155959 |
url |
http://dx.doi.org/10.1007/s00497-017-0300-7 http://hdl.handle.net/11449/162891 |
identifier_str_mv |
Plant Reproduction. New York: Springer, v. 30, n. 2, p. 69-79, 2017. 2194-7953 10.1007/s00497-017-0300-7 WOS:000403546900001 WOS000403546900001.pdf 7010826495155959 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plant Reproduction 1,597 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
69-79 application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129236351844352 |