Deciphering the role of ttrA and pduA genes for Salmonella enterica serovars in a chicken infection model
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/03079457.2021.1909703 http://hdl.handle.net/11449/207703 |
Resumo: | Salmonella enterica serovars use self-induced intestinal inflammation to increase electron acceptor availability and to obtain a growth advantage in the host gut. There is evidence suggesting that the ability of Salmonella to use tetrathionate and 1,2-propanediol provides an advantage in murine infection. Thus, we present here the first study to evaluate both systemic infection and faecal excretion in commercial poultry challenged by Salmonella Enteritidis (SE) and S. Typhimurium (STM) harbouring deletions in ttrA and pduA genes, which are crucial to the metabolism of tetrathionate and 1,2-propanediol, respectively. Mutant strains were excreted at higher rates when compared to the wild-type strains. The highest rates were observed with white egg-layer and brown egg-layer chicks (67.5%), and broiler chicks (56.7%) challenged by SEΔttrAΔpduA, and brown egg-layer chicks (64.8%) challenged by STMΔttrAΔpduA. SEΔttrAΔpduA presented higher bacterial counts in the liver and spleen of the three chicken lineages and caecal contents from the broiler chickens, whereas STMΔttrAΔpduA presented higher counts in the liver and spleen of the broiler and brown-egg chickens for 28 days post-infection (P < 0.05). The ttrA and pduA genes do not appear to be major virulence determinants in faecal excretion or invasiveness for SE and STM in chickens. RESEARCH HIGHLIGHTSttrA and pudA do not impair gut colonization or systemic infection in chicks. Mutant strains were present in higher numbers in broilers than in laying chicks. Mutants of SE and STM showed greater pathogenicity in broiler chicks than layers. |
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Deciphering the role of ttrA and pduA genes for Salmonella enterica serovars in a chicken infection model1,2-propanediolparatyphoid infectionpoultrySalmonella EnteritidisSalmonella TyphimuriumsalmonellosistetrathionateSalmonella enterica serovars use self-induced intestinal inflammation to increase electron acceptor availability and to obtain a growth advantage in the host gut. There is evidence suggesting that the ability of Salmonella to use tetrathionate and 1,2-propanediol provides an advantage in murine infection. Thus, we present here the first study to evaluate both systemic infection and faecal excretion in commercial poultry challenged by Salmonella Enteritidis (SE) and S. Typhimurium (STM) harbouring deletions in ttrA and pduA genes, which are crucial to the metabolism of tetrathionate and 1,2-propanediol, respectively. Mutant strains were excreted at higher rates when compared to the wild-type strains. The highest rates were observed with white egg-layer and brown egg-layer chicks (67.5%), and broiler chicks (56.7%) challenged by SEΔttrAΔpduA, and brown egg-layer chicks (64.8%) challenged by STMΔttrAΔpduA. SEΔttrAΔpduA presented higher bacterial counts in the liver and spleen of the three chicken lineages and caecal contents from the broiler chickens, whereas STMΔttrAΔpduA presented higher counts in the liver and spleen of the broiler and brown-egg chickens for 28 days post-infection (P < 0.05). The ttrA and pduA genes do not appear to be major virulence determinants in faecal excretion or invasiveness for SE and STM in chickens. RESEARCH HIGHLIGHTSttrA and pudA do not impair gut colonization or systemic infection in chicks. Mutant strains were present in higher numbers in broilers than in laying chicks. Mutants of SE and STM showed greater pathogenicity in broiler chicks than layers.Laboratory of Avian Pathology Department of Pathology Theriogenology and One Health Sao Paulo State University (FCAV-Unesp)Department of Preventive Veterinary Medicine Veterinary School Federal University of Minas Gerais (UFMG)School of Veterinary Medicine and Science University of SurreyLaboratory of Avian Pathology Department of Pathology Theriogenology and One Health Sao Paulo State University (FCAV-Unesp)Universidade Estadual Paulista (Unesp)Universidade Federal de Minas Gerais (UFMG)University of SurreySaraiva, M. M.S. [UNESP]Rodrigues Alves, L. B. [UNESP]Monte, D. F.M. [UNESP]Ferreira, T. S. [UNESP]Benevides, V. P. [UNESP]Barbosa, F. O. [UNESP]Freitas Neto, O. C.Almeida, A. M. [UNESP]Barrow, P. A.Berchieri Junior, A. [UNESP]2021-06-25T10:59:32Z2021-06-25T10:59:32Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1080/03079457.2021.1909703Avian Pathology.1465-33380307-9457http://hdl.handle.net/11449/20770310.1080/03079457.2021.19097032-s2.0-85105394526Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAvian Pathologyinfo:eu-repo/semantics/openAccess2021-10-23T17:45:56Zoai:repositorio.unesp.br:11449/207703Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T17:45:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Deciphering the role of ttrA and pduA genes for Salmonella enterica serovars in a chicken infection model |
title |
Deciphering the role of ttrA and pduA genes for Salmonella enterica serovars in a chicken infection model |
spellingShingle |
Deciphering the role of ttrA and pduA genes for Salmonella enterica serovars in a chicken infection model Saraiva, M. M.S. [UNESP] 1,2-propanediol paratyphoid infection poultry Salmonella Enteritidis Salmonella Typhimurium salmonellosis tetrathionate |
title_short |
Deciphering the role of ttrA and pduA genes for Salmonella enterica serovars in a chicken infection model |
title_full |
Deciphering the role of ttrA and pduA genes for Salmonella enterica serovars in a chicken infection model |
title_fullStr |
Deciphering the role of ttrA and pduA genes for Salmonella enterica serovars in a chicken infection model |
title_full_unstemmed |
Deciphering the role of ttrA and pduA genes for Salmonella enterica serovars in a chicken infection model |
title_sort |
Deciphering the role of ttrA and pduA genes for Salmonella enterica serovars in a chicken infection model |
author |
Saraiva, M. M.S. [UNESP] |
author_facet |
Saraiva, M. M.S. [UNESP] Rodrigues Alves, L. B. [UNESP] Monte, D. F.M. [UNESP] Ferreira, T. S. [UNESP] Benevides, V. P. [UNESP] Barbosa, F. O. [UNESP] Freitas Neto, O. C. Almeida, A. M. [UNESP] Barrow, P. A. Berchieri Junior, A. [UNESP] |
author_role |
author |
author2 |
Rodrigues Alves, L. B. [UNESP] Monte, D. F.M. [UNESP] Ferreira, T. S. [UNESP] Benevides, V. P. [UNESP] Barbosa, F. O. [UNESP] Freitas Neto, O. C. Almeida, A. M. [UNESP] Barrow, P. A. Berchieri Junior, A. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Minas Gerais (UFMG) University of Surrey |
dc.contributor.author.fl_str_mv |
Saraiva, M. M.S. [UNESP] Rodrigues Alves, L. B. [UNESP] Monte, D. F.M. [UNESP] Ferreira, T. S. [UNESP] Benevides, V. P. [UNESP] Barbosa, F. O. [UNESP] Freitas Neto, O. C. Almeida, A. M. [UNESP] Barrow, P. A. Berchieri Junior, A. [UNESP] |
dc.subject.por.fl_str_mv |
1,2-propanediol paratyphoid infection poultry Salmonella Enteritidis Salmonella Typhimurium salmonellosis tetrathionate |
topic |
1,2-propanediol paratyphoid infection poultry Salmonella Enteritidis Salmonella Typhimurium salmonellosis tetrathionate |
description |
Salmonella enterica serovars use self-induced intestinal inflammation to increase electron acceptor availability and to obtain a growth advantage in the host gut. There is evidence suggesting that the ability of Salmonella to use tetrathionate and 1,2-propanediol provides an advantage in murine infection. Thus, we present here the first study to evaluate both systemic infection and faecal excretion in commercial poultry challenged by Salmonella Enteritidis (SE) and S. Typhimurium (STM) harbouring deletions in ttrA and pduA genes, which are crucial to the metabolism of tetrathionate and 1,2-propanediol, respectively. Mutant strains were excreted at higher rates when compared to the wild-type strains. The highest rates were observed with white egg-layer and brown egg-layer chicks (67.5%), and broiler chicks (56.7%) challenged by SEΔttrAΔpduA, and brown egg-layer chicks (64.8%) challenged by STMΔttrAΔpduA. SEΔttrAΔpduA presented higher bacterial counts in the liver and spleen of the three chicken lineages and caecal contents from the broiler chickens, whereas STMΔttrAΔpduA presented higher counts in the liver and spleen of the broiler and brown-egg chickens for 28 days post-infection (P < 0.05). The ttrA and pduA genes do not appear to be major virulence determinants in faecal excretion or invasiveness for SE and STM in chickens. RESEARCH HIGHLIGHTSttrA and pudA do not impair gut colonization or systemic infection in chicks. Mutant strains were present in higher numbers in broilers than in laying chicks. Mutants of SE and STM showed greater pathogenicity in broiler chicks than layers. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:59:32Z 2021-06-25T10:59:32Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/03079457.2021.1909703 Avian Pathology. 1465-3338 0307-9457 http://hdl.handle.net/11449/207703 10.1080/03079457.2021.1909703 2-s2.0-85105394526 |
url |
http://dx.doi.org/10.1080/03079457.2021.1909703 http://hdl.handle.net/11449/207703 |
identifier_str_mv |
Avian Pathology. 1465-3338 0307-9457 10.1080/03079457.2021.1909703 2-s2.0-85105394526 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Avian Pathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965562491633664 |