Micropropagação de babosa (Aloe vera L.)
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo6_v9_n1.pdf http://hdl.handle.net/11449/69527 |
Resumo: | The demand for hiah aenetic and fit sanitary quality plantlets of medicinal plants is increasing. The babosa (Aloe vera L.) is propagated conventionally through lateral buds, which is a slowly, very expensive and low income practice. Thus, the objective of this work was the development of A. vera micropropagation protocol. Meristems of A. vera were submitted to asepsis tests, varying the time of immersion in 3% NaOCI solution, 70% alcohol solution and after were submitted in vitro multiplication with effect of combinations and concentrations of vegetal regulators BA (6-Benzylaminopurine), KIN (Kinetin), IAA (lndole-3-acetic acid) and NAA (á Naphthyl acetic acid) supplemented in the MS media culture during 180 days (6 subcultures). For the initial establishment, the best disinfestations treatment was the meristem immersion for 20 minutes in 3% NaOCI solution + 1 minute in 70% alcohol solution, repeating that sequence in the board and after in the laminar flow. In relation to in vitro multiplication, the best results were the use of MS media culture with out vegetal regulators in the first subculture (30 days), supplemented with 2.85 mmol L -1 of IAA + 4.44 mmol L -1 of BA in the second subculture (60 days), 2.85 mmol L -1 of IAA+ 8.88 mmol L -1 of BA in the third subculture (90 days), 2.85 mmol L -1 of IAA+22.2 mmol L -1 of BA in the fourth subculture (120 days), 4.44 mmol/L of BA in the fifth subculture (150 days) and again removing the vegetal regulators in the sixth subculture (180 days), reaching an average of 201 new plantlets of A. vera in a period of 6 months. |
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Micropropagação de babosa (Aloe vera L.)Micropropagation of Aloe vera L.AloeIn vitro cloningMedicinal plantsPlant growth regulatorPropagation6 n benzyladeninekinetinAloe veraasepsisin vitro studylaminar flowmeristemmicropropagationplant growthThe demand for hiah aenetic and fit sanitary quality plantlets of medicinal plants is increasing. The babosa (Aloe vera L.) is propagated conventionally through lateral buds, which is a slowly, very expensive and low income practice. Thus, the objective of this work was the development of A. vera micropropagation protocol. Meristems of A. vera were submitted to asepsis tests, varying the time of immersion in 3% NaOCI solution, 70% alcohol solution and after were submitted in vitro multiplication with effect of combinations and concentrations of vegetal regulators BA (6-Benzylaminopurine), KIN (Kinetin), IAA (lndole-3-acetic acid) and NAA (á Naphthyl acetic acid) supplemented in the MS media culture during 180 days (6 subcultures). For the initial establishment, the best disinfestations treatment was the meristem immersion for 20 minutes in 3% NaOCI solution + 1 minute in 70% alcohol solution, repeating that sequence in the board and after in the laminar flow. In relation to in vitro multiplication, the best results were the use of MS media culture with out vegetal regulators in the first subculture (30 days), supplemented with 2.85 mmol L -1 of IAA + 4.44 mmol L -1 of BA in the second subculture (60 days), 2.85 mmol L -1 of IAA+ 8.88 mmol L -1 of BA in the third subculture (90 days), 2.85 mmol L -1 of IAA+22.2 mmol L -1 of BA in the fourth subculture (120 days), 4.44 mmol/L of BA in the fifth subculture (150 days) and again removing the vegetal regulators in the sixth subculture (180 days), reaching an average of 201 new plantlets of A. vera in a period of 6 months.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FURB - Fundação Universidade Regional de Blumenau, Blumenau-SC, 89010-971CNPqUNESP - Universidade Estadual Paulista, Botucatu-SP, 18610-307UNESP - Universidade Estadual Paulista, Botucatu-SP, 18610-307FURB - Fundação Universidade Regional de BlumenauUniversidade Estadual Paulista (Unesp)Debiasi, C. [UNESP]Silva, C. G.Pescador, R.2014-05-27T11:22:24Z2014-05-27T11:22:24Z2007-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article36-43application/pdfhttp://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo6_v9_n1.pdfRevista Brasileira de Plantas Medicinais, v. 9, n. 1, p. 36-43, 2007.1516-0572http://hdl.handle.net/11449/695272-s2.0-342481402302-s2.0-34248140230.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Plantas Medicinais0,199info:eu-repo/semantics/openAccess2023-12-16T06:17:49Zoai:repositorio.unesp.br:11449/69527Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:28:03.127168Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Micropropagação de babosa (Aloe vera L.) Micropropagation of Aloe vera L. |
title |
Micropropagação de babosa (Aloe vera L.) |
spellingShingle |
Micropropagação de babosa (Aloe vera L.) Debiasi, C. [UNESP] Aloe In vitro cloning Medicinal plants Plant growth regulator Propagation 6 n benzyladenine kinetin Aloe vera asepsis in vitro study laminar flow meristem micropropagation plant growth |
title_short |
Micropropagação de babosa (Aloe vera L.) |
title_full |
Micropropagação de babosa (Aloe vera L.) |
title_fullStr |
Micropropagação de babosa (Aloe vera L.) |
title_full_unstemmed |
Micropropagação de babosa (Aloe vera L.) |
title_sort |
Micropropagação de babosa (Aloe vera L.) |
author |
Debiasi, C. [UNESP] |
author_facet |
Debiasi, C. [UNESP] Silva, C. G. Pescador, R. |
author_role |
author |
author2 |
Silva, C. G. Pescador, R. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
FURB - Fundação Universidade Regional de Blumenau Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Debiasi, C. [UNESP] Silva, C. G. Pescador, R. |
dc.subject.por.fl_str_mv |
Aloe In vitro cloning Medicinal plants Plant growth regulator Propagation 6 n benzyladenine kinetin Aloe vera asepsis in vitro study laminar flow meristem micropropagation plant growth |
topic |
Aloe In vitro cloning Medicinal plants Plant growth regulator Propagation 6 n benzyladenine kinetin Aloe vera asepsis in vitro study laminar flow meristem micropropagation plant growth |
description |
The demand for hiah aenetic and fit sanitary quality plantlets of medicinal plants is increasing. The babosa (Aloe vera L.) is propagated conventionally through lateral buds, which is a slowly, very expensive and low income practice. Thus, the objective of this work was the development of A. vera micropropagation protocol. Meristems of A. vera were submitted to asepsis tests, varying the time of immersion in 3% NaOCI solution, 70% alcohol solution and after were submitted in vitro multiplication with effect of combinations and concentrations of vegetal regulators BA (6-Benzylaminopurine), KIN (Kinetin), IAA (lndole-3-acetic acid) and NAA (á Naphthyl acetic acid) supplemented in the MS media culture during 180 days (6 subcultures). For the initial establishment, the best disinfestations treatment was the meristem immersion for 20 minutes in 3% NaOCI solution + 1 minute in 70% alcohol solution, repeating that sequence in the board and after in the laminar flow. In relation to in vitro multiplication, the best results were the use of MS media culture with out vegetal regulators in the first subculture (30 days), supplemented with 2.85 mmol L -1 of IAA + 4.44 mmol L -1 of BA in the second subculture (60 days), 2.85 mmol L -1 of IAA+ 8.88 mmol L -1 of BA in the third subculture (90 days), 2.85 mmol L -1 of IAA+22.2 mmol L -1 of BA in the fourth subculture (120 days), 4.44 mmol/L of BA in the fifth subculture (150 days) and again removing the vegetal regulators in the sixth subculture (180 days), reaching an average of 201 new plantlets of A. vera in a period of 6 months. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-02-01 2014-05-27T11:22:24Z 2014-05-27T11:22:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo6_v9_n1.pdf Revista Brasileira de Plantas Medicinais, v. 9, n. 1, p. 36-43, 2007. 1516-0572 http://hdl.handle.net/11449/69527 2-s2.0-34248140230 2-s2.0-34248140230.pdf |
url |
http://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo6_v9_n1.pdf http://hdl.handle.net/11449/69527 |
identifier_str_mv |
Revista Brasileira de Plantas Medicinais, v. 9, n. 1, p. 36-43, 2007. 1516-0572 2-s2.0-34248140230 2-s2.0-34248140230.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista Brasileira de Plantas Medicinais 0,199 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
36-43 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129205612838912 |