MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM

Detalhes bibliográficos
Autor(a) principal: Montanari, Lilian Bueno
Data de Publicação: 2009
Outros Autores: Sartori, Flavio Garcia, de Oliveira Cardoso, Miguel Jorge, Varo, Samuel Dutra, Pires, Regina Helena, Leite, Clarice Queico Fujimura [UNESP], Prince, Karina [UNESP], Gomes Martins, Carlos Henrique
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0036-46652009000100007
http://hdl.handle.net/11449/7433
Resumo: The microbiological monitoring of the water used for hemodialysis is extremely important, especially because of the debilitated immune system of patients suffering from chronic renal insufficiency. To investigate the occurrence and species diversity of bacteria in waters, water samples were collected monthly from a hemodialysis center in upstate São Paulo and tap water samples at the terminal sites of the distribution system was sampled repeatedly (22 times) at each of five points in the distribution system; a further 36 samples were taken from cannulae in 19 hemodialysis machines that were ready for the next patient, four samples from the reuse system and 13 from the water storage system. To identify bacteria, samples were filtered through 0.22 mu m-pore membranes; for mycobacteria, 0.45 mu m pores were used. Conventional microbiological and molecular methods were used in the analysis. Bacteria were isolated from the distribution system (128 isolates), kidney machine water (43) and reuse system (3). Among these isolates, 32 were Gram-positive rods, 120 Gram-negative rods, 20 Gram-positive cocci and 11 mycobacteria. We propose the continual monitoring of the water supplies in hemodialysis centers and the adoption of effective prophylactic measures that minimize the exposure of these immunodeficient patients to contaminated sources of water.
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spelling MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEMDialysisWaterMicrobiological contaminationWater treatmentDialysis fluid qualityBacterial countThe microbiological monitoring of the water used for hemodialysis is extremely important, especially because of the debilitated immune system of patients suffering from chronic renal insufficiency. To investigate the occurrence and species diversity of bacteria in waters, water samples were collected monthly from a hemodialysis center in upstate São Paulo and tap water samples at the terminal sites of the distribution system was sampled repeatedly (22 times) at each of five points in the distribution system; a further 36 samples were taken from cannulae in 19 hemodialysis machines that were ready for the next patient, four samples from the reuse system and 13 from the water storage system. To identify bacteria, samples were filtered through 0.22 mu m-pore membranes; for mycobacteria, 0.45 mu m pores were used. Conventional microbiological and molecular methods were used in the analysis. Bacteria were isolated from the distribution system (128 isolates), kidney machine water (43) and reuse system (3). Among these isolates, 32 were Gram-positive rods, 120 Gram-negative rods, 20 Gram-positive cocci and 11 mycobacteria. We propose the continual monitoring of the water supplies in hemodialysis centers and the adoption of effective prophylactic measures that minimize the exposure of these immunodeficient patients to contaminated sources of water.Univ Franca, Lab Res Appl Microbiol, Franca, SP, BrazilState Univ São Paulo, Dept Biol Sci, Araraquara, SP, BrazilState Univ São Paulo, Dept Biol Sci, Araraquara, SP, BrazilInst Medicina Tropical São PauloUniv FrancaUniversidade Estadual Paulista (Unesp)Montanari, Lilian BuenoSartori, Flavio Garciade Oliveira Cardoso, Miguel JorgeVaro, Samuel DutraPires, Regina HelenaLeite, Clarice Queico Fujimura [UNESP]Prince, Karina [UNESP]Gomes Martins, Carlos Henrique2014-05-20T13:24:11Z2014-05-20T13:24:11Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article37-43http://dx.doi.org/10.1590/S0036-46652009000100007Revista do Instituto de Medicina Tropical de São Paulo. São Paulo: Inst Medicina Tropical São Paulo, v. 51, n. 1, p. 37-43, 2009.0036-4665http://hdl.handle.net/11449/743310.1590/S0036-46652009000100007S0036-46652009000100007WOS:000263551900007S0036-46652009000100007.pdf2114570774349859Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista do Instituto de Medicina Tropical de São Paulo1.4890,669info:eu-repo/semantics/openAccess2024-06-24T13:07:51Zoai:repositorio.unesp.br:11449/7433Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:00:36.687580Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM
title MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM
spellingShingle MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM
Montanari, Lilian Bueno
Dialysis
Water
Microbiological contamination
Water treatment
Dialysis fluid quality
Bacterial count
title_short MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM
title_full MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM
title_fullStr MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM
title_full_unstemmed MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM
title_sort MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM
author Montanari, Lilian Bueno
author_facet Montanari, Lilian Bueno
Sartori, Flavio Garcia
de Oliveira Cardoso, Miguel Jorge
Varo, Samuel Dutra
Pires, Regina Helena
Leite, Clarice Queico Fujimura [UNESP]
Prince, Karina [UNESP]
Gomes Martins, Carlos Henrique
author_role author
author2 Sartori, Flavio Garcia
de Oliveira Cardoso, Miguel Jorge
Varo, Samuel Dutra
Pires, Regina Helena
Leite, Clarice Queico Fujimura [UNESP]
Prince, Karina [UNESP]
Gomes Martins, Carlos Henrique
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Franca
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Montanari, Lilian Bueno
Sartori, Flavio Garcia
de Oliveira Cardoso, Miguel Jorge
Varo, Samuel Dutra
Pires, Regina Helena
Leite, Clarice Queico Fujimura [UNESP]
Prince, Karina [UNESP]
Gomes Martins, Carlos Henrique
dc.subject.por.fl_str_mv Dialysis
Water
Microbiological contamination
Water treatment
Dialysis fluid quality
Bacterial count
topic Dialysis
Water
Microbiological contamination
Water treatment
Dialysis fluid quality
Bacterial count
description The microbiological monitoring of the water used for hemodialysis is extremely important, especially because of the debilitated immune system of patients suffering from chronic renal insufficiency. To investigate the occurrence and species diversity of bacteria in waters, water samples were collected monthly from a hemodialysis center in upstate São Paulo and tap water samples at the terminal sites of the distribution system was sampled repeatedly (22 times) at each of five points in the distribution system; a further 36 samples were taken from cannulae in 19 hemodialysis machines that were ready for the next patient, four samples from the reuse system and 13 from the water storage system. To identify bacteria, samples were filtered through 0.22 mu m-pore membranes; for mycobacteria, 0.45 mu m pores were used. Conventional microbiological and molecular methods were used in the analysis. Bacteria were isolated from the distribution system (128 isolates), kidney machine water (43) and reuse system (3). Among these isolates, 32 were Gram-positive rods, 120 Gram-negative rods, 20 Gram-positive cocci and 11 mycobacteria. We propose the continual monitoring of the water supplies in hemodialysis centers and the adoption of effective prophylactic measures that minimize the exposure of these immunodeficient patients to contaminated sources of water.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
2014-05-20T13:24:11Z
2014-05-20T13:24:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0036-46652009000100007
Revista do Instituto de Medicina Tropical de São Paulo. São Paulo: Inst Medicina Tropical São Paulo, v. 51, n. 1, p. 37-43, 2009.
0036-4665
http://hdl.handle.net/11449/7433
10.1590/S0036-46652009000100007
S0036-46652009000100007
WOS:000263551900007
S0036-46652009000100007.pdf
2114570774349859
url http://dx.doi.org/10.1590/S0036-46652009000100007
http://hdl.handle.net/11449/7433
identifier_str_mv Revista do Instituto de Medicina Tropical de São Paulo. São Paulo: Inst Medicina Tropical São Paulo, v. 51, n. 1, p. 37-43, 2009.
0036-4665
10.1590/S0036-46652009000100007
S0036-46652009000100007
WOS:000263551900007
S0036-46652009000100007.pdf
2114570774349859
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo
1.489
0,669
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 37-43
dc.publisher.none.fl_str_mv Inst Medicina Tropical São Paulo
publisher.none.fl_str_mv Inst Medicina Tropical São Paulo
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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