MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0036-46652009000100007 http://hdl.handle.net/11449/7433 |
Resumo: | The microbiological monitoring of the water used for hemodialysis is extremely important, especially because of the debilitated immune system of patients suffering from chronic renal insufficiency. To investigate the occurrence and species diversity of bacteria in waters, water samples were collected monthly from a hemodialysis center in upstate São Paulo and tap water samples at the terminal sites of the distribution system was sampled repeatedly (22 times) at each of five points in the distribution system; a further 36 samples were taken from cannulae in 19 hemodialysis machines that were ready for the next patient, four samples from the reuse system and 13 from the water storage system. To identify bacteria, samples were filtered through 0.22 mu m-pore membranes; for mycobacteria, 0.45 mu m pores were used. Conventional microbiological and molecular methods were used in the analysis. Bacteria were isolated from the distribution system (128 isolates), kidney machine water (43) and reuse system (3). Among these isolates, 32 were Gram-positive rods, 120 Gram-negative rods, 20 Gram-positive cocci and 11 mycobacteria. We propose the continual monitoring of the water supplies in hemodialysis centers and the adoption of effective prophylactic measures that minimize the exposure of these immunodeficient patients to contaminated sources of water. |
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MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEMDialysisWaterMicrobiological contaminationWater treatmentDialysis fluid qualityBacterial countThe microbiological monitoring of the water used for hemodialysis is extremely important, especially because of the debilitated immune system of patients suffering from chronic renal insufficiency. To investigate the occurrence and species diversity of bacteria in waters, water samples were collected monthly from a hemodialysis center in upstate São Paulo and tap water samples at the terminal sites of the distribution system was sampled repeatedly (22 times) at each of five points in the distribution system; a further 36 samples were taken from cannulae in 19 hemodialysis machines that were ready for the next patient, four samples from the reuse system and 13 from the water storage system. To identify bacteria, samples were filtered through 0.22 mu m-pore membranes; for mycobacteria, 0.45 mu m pores were used. Conventional microbiological and molecular methods were used in the analysis. Bacteria were isolated from the distribution system (128 isolates), kidney machine water (43) and reuse system (3). Among these isolates, 32 were Gram-positive rods, 120 Gram-negative rods, 20 Gram-positive cocci and 11 mycobacteria. We propose the continual monitoring of the water supplies in hemodialysis centers and the adoption of effective prophylactic measures that minimize the exposure of these immunodeficient patients to contaminated sources of water.Univ Franca, Lab Res Appl Microbiol, Franca, SP, BrazilState Univ São Paulo, Dept Biol Sci, Araraquara, SP, BrazilState Univ São Paulo, Dept Biol Sci, Araraquara, SP, BrazilInst Medicina Tropical São PauloUniv FrancaUniversidade Estadual Paulista (Unesp)Montanari, Lilian BuenoSartori, Flavio Garciade Oliveira Cardoso, Miguel JorgeVaro, Samuel DutraPires, Regina HelenaLeite, Clarice Queico Fujimura [UNESP]Prince, Karina [UNESP]Gomes Martins, Carlos Henrique2014-05-20T13:24:11Z2014-05-20T13:24:11Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article37-43http://dx.doi.org/10.1590/S0036-46652009000100007Revista do Instituto de Medicina Tropical de São Paulo. São Paulo: Inst Medicina Tropical São Paulo, v. 51, n. 1, p. 37-43, 2009.0036-4665http://hdl.handle.net/11449/743310.1590/S0036-46652009000100007S0036-46652009000100007WOS:000263551900007S0036-46652009000100007.pdf2114570774349859Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista do Instituto de Medicina Tropical de São Paulo1.4890,669info:eu-repo/semantics/openAccess2024-06-24T13:07:51Zoai:repositorio.unesp.br:11449/7433Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:00:36.687580Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM |
title |
MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM |
spellingShingle |
MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM Montanari, Lilian Bueno Dialysis Water Microbiological contamination Water treatment Dialysis fluid quality Bacterial count |
title_short |
MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM |
title_full |
MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM |
title_fullStr |
MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM |
title_full_unstemmed |
MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM |
title_sort |
MICROBIOLOGICAL CONTAMINATION of A HEMODIALYSIS CENTER WATER DISTRIBUTION SYSTEM |
author |
Montanari, Lilian Bueno |
author_facet |
Montanari, Lilian Bueno Sartori, Flavio Garcia de Oliveira Cardoso, Miguel Jorge Varo, Samuel Dutra Pires, Regina Helena Leite, Clarice Queico Fujimura [UNESP] Prince, Karina [UNESP] Gomes Martins, Carlos Henrique |
author_role |
author |
author2 |
Sartori, Flavio Garcia de Oliveira Cardoso, Miguel Jorge Varo, Samuel Dutra Pires, Regina Helena Leite, Clarice Queico Fujimura [UNESP] Prince, Karina [UNESP] Gomes Martins, Carlos Henrique |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Franca Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Montanari, Lilian Bueno Sartori, Flavio Garcia de Oliveira Cardoso, Miguel Jorge Varo, Samuel Dutra Pires, Regina Helena Leite, Clarice Queico Fujimura [UNESP] Prince, Karina [UNESP] Gomes Martins, Carlos Henrique |
dc.subject.por.fl_str_mv |
Dialysis Water Microbiological contamination Water treatment Dialysis fluid quality Bacterial count |
topic |
Dialysis Water Microbiological contamination Water treatment Dialysis fluid quality Bacterial count |
description |
The microbiological monitoring of the water used for hemodialysis is extremely important, especially because of the debilitated immune system of patients suffering from chronic renal insufficiency. To investigate the occurrence and species diversity of bacteria in waters, water samples were collected monthly from a hemodialysis center in upstate São Paulo and tap water samples at the terminal sites of the distribution system was sampled repeatedly (22 times) at each of five points in the distribution system; a further 36 samples were taken from cannulae in 19 hemodialysis machines that were ready for the next patient, four samples from the reuse system and 13 from the water storage system. To identify bacteria, samples were filtered through 0.22 mu m-pore membranes; for mycobacteria, 0.45 mu m pores were used. Conventional microbiological and molecular methods were used in the analysis. Bacteria were isolated from the distribution system (128 isolates), kidney machine water (43) and reuse system (3). Among these isolates, 32 were Gram-positive rods, 120 Gram-negative rods, 20 Gram-positive cocci and 11 mycobacteria. We propose the continual monitoring of the water supplies in hemodialysis centers and the adoption of effective prophylactic measures that minimize the exposure of these immunodeficient patients to contaminated sources of water. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 2014-05-20T13:24:11Z 2014-05-20T13:24:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0036-46652009000100007 Revista do Instituto de Medicina Tropical de São Paulo. São Paulo: Inst Medicina Tropical São Paulo, v. 51, n. 1, p. 37-43, 2009. 0036-4665 http://hdl.handle.net/11449/7433 10.1590/S0036-46652009000100007 S0036-46652009000100007 WOS:000263551900007 S0036-46652009000100007.pdf 2114570774349859 |
url |
http://dx.doi.org/10.1590/S0036-46652009000100007 http://hdl.handle.net/11449/7433 |
identifier_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo. São Paulo: Inst Medicina Tropical São Paulo, v. 51, n. 1, p. 37-43, 2009. 0036-4665 10.1590/S0036-46652009000100007 S0036-46652009000100007 WOS:000263551900007 S0036-46652009000100007.pdf 2114570774349859 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo 1.489 0,669 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
37-43 |
dc.publisher.none.fl_str_mv |
Inst Medicina Tropical São Paulo |
publisher.none.fl_str_mv |
Inst Medicina Tropical São Paulo |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129011652493312 |