Ação sinérgica de componentes da própolis sobre produção de citocinas e atividade bactericida de monócitos humanos

• Autor(a) principal: Cardoso, Eliza de Oliveira [UNESP]
• Data de Publicação: 2014
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/145393; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2016-09-20/000869512.pdf
• topic: Própole - Uso terapêutico; Citocinas; Monocitos; Fatores imunológicos; Própolis

Propolis is a natural product with several biological properties. It shows a very complex chemical composition, what may change according to the botanical sources. In the last decades researchers have focused on the activity of its components, in order to investigate their involvement in propolis action. However, little is known concerning the synergism of its constituents on the immune system. Thus, this work aimed to investigate whether the immunomodulatory effects of propolis on human monocytes are due to the interaction between three phenolic acids (caffeic, dihydrocinnamic and p-coumaric acid). Human monocytes were obtained from 10 healthy donors and incubated with such components and their combinations (cafeic+dihydrocinnamic; caffeic+p-coumaric; dihydrocinnamic+p-coumaric; and caffeic+dihydrocinnamic+pcoumaric) according to their concentrations in propolis composition, simultaneously or not with LPS. A possible cytotoxicity was assessed by the colorimetric MTT assay. After 18h incubation, supernatants were collected and the production of pro- and antiinflammatory cytokines (TNF-α and IL-10) was evaluated by enzyme-linked immunosorbent assay (ELISA). To evaluate the bactericidal activity, monocytes were incubated with components and challenged with Escherichia coli, and the percentage of bactericidal activity was calculated. Significant differences between treatments were determined by analysis of variance (ANOVA), followed by Dunnett's test (P<0.05). Without LPS, propolis and the associations between its acids stimulated the cytokines production. With inflammatory stimulus, the LPS-induce TNF-α and IL-10 production was maintained by propolis, its acids and their associations. Cinnamic acid increased IL-10 production and the bactericidal activity of monocytes, without affecting cell viability. These preliminary results suggest that these compounds may be involved in the immunomodulatory action of propolis, but further studies on the...