Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-021-98713-2 http://hdl.handle.net/11449/233616 |
Resumo: | This study investigated the role 5-lypoxigenase (5-LO) on alveolar socket healing in aged female mice treated with zoledronic acid (ZL). Forty 129/Sv female mice (64–68 weeks old), 20 wild type (WT) and 20 5-LO knockout (5LOKO) were equally distributed according to ZL treatment: WT Control, WT ZL, 5LOKO Control, and 5LOKO ZL. ZL groups were treated with an intraperitoneal injection of 250 µg/Kg of ZL, while controls were treated with saline. Treatments were administered once a week, starting four weeks before surgery for tooth extraction and until 7 and 21 days post-surgery. Mice were euthanized for a comprehensive microscopic analysis (microCT, histomorphometry and immunohistochemistry). WT ZL mice presented intense inflammatory infiltrate (7 days), delayed bone formation (21 days), reduced collagenous matrix quality, and a deficiency in Runx-2 + , TRAP + , and macrophages as compared to controls. 5LOKO ZL animals presented decreased number of Runx-2 + cells in comparison to 5LOKO Control at 7 days, but no major changes in bone healing as compared to WT or 5LOKO mice at 21 days. The knockout of 5LO favored intramembranous bone healing in aged female mice, with a direct impact on inflammatory response and bone metabolism on the development of ONJ-like lesions. |
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Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acidThis study investigated the role 5-lypoxigenase (5-LO) on alveolar socket healing in aged female mice treated with zoledronic acid (ZL). Forty 129/Sv female mice (64–68 weeks old), 20 wild type (WT) and 20 5-LO knockout (5LOKO) were equally distributed according to ZL treatment: WT Control, WT ZL, 5LOKO Control, and 5LOKO ZL. ZL groups were treated with an intraperitoneal injection of 250 µg/Kg of ZL, while controls were treated with saline. Treatments were administered once a week, starting four weeks before surgery for tooth extraction and until 7 and 21 days post-surgery. Mice were euthanized for a comprehensive microscopic analysis (microCT, histomorphometry and immunohistochemistry). WT ZL mice presented intense inflammatory infiltrate (7 days), delayed bone formation (21 days), reduced collagenous matrix quality, and a deficiency in Runx-2 + , TRAP + , and macrophages as compared to controls. 5LOKO ZL animals presented decreased number of Runx-2 + cells in comparison to 5LOKO Control at 7 days, but no major changes in bone healing as compared to WT or 5LOKO mice at 21 days. The knockout of 5LO favored intramembranous bone healing in aged female mice, with a direct impact on inflammatory response and bone metabolism on the development of ONJ-like lesions.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Basic Sciences School of Dentistry São Paulo State University (UNESP)Department of Bioengineering University of Texas at DallasDepartment of Health Sciences Centro Universitário Sagrado CoraçãoBauru School of Dentistry University of São Paulo FOB-USPDepartment of Surgery School of Dentistry São Paulo State University (UNESP)Center for Craniofacial Research University of Texas Health Science Center at Houston School of DentistryDepartment of Basic Sciences School of Dentistry São Paulo State University (UNESP)Department of Surgery School of Dentistry São Paulo State University (UNESP)FAPESP: #2018/08913-9FAPESP: #2018/19409-0FAPESP: #2019/16067-3Universidade Estadual Paulista (UNESP)University of Texas at DallasCentro Universitário Sagrado CoraçãoUniversidade de São Paulo (USP)School of DentistryMahmoud, Ramez H. [UNESP]Biguetti, Claudia C.Simionato, Gustavo B. [UNESP]Custódio, Isabela C.Silva, Raquel B. P. [UNESP]Duarte, Marco A. H.Faverani, Leonardo P. [UNESP]Ervolino, Edilson [UNESP]Fakhouri, Walid D.Matsumoto, Mariza A. [UNESP]2022-05-01T09:30:59Z2022-05-01T09:30:59Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-021-98713-2Scientific Reports, v. 11, n. 1, 2021.2045-2322http://hdl.handle.net/11449/23361610.1038/s41598-021-98713-22-s2.0-85116365055Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2022-05-01T09:30:59Zoai:repositorio.unesp.br:11449/233616Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:45:41.049877Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid |
title |
Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid |
spellingShingle |
Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid Mahmoud, Ramez H. [UNESP] |
title_short |
Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid |
title_full |
Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid |
title_fullStr |
Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid |
title_full_unstemmed |
Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid |
title_sort |
Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid |
author |
Mahmoud, Ramez H. [UNESP] |
author_facet |
Mahmoud, Ramez H. [UNESP] Biguetti, Claudia C. Simionato, Gustavo B. [UNESP] Custódio, Isabela C. Silva, Raquel B. P. [UNESP] Duarte, Marco A. H. Faverani, Leonardo P. [UNESP] Ervolino, Edilson [UNESP] Fakhouri, Walid D. Matsumoto, Mariza A. [UNESP] |
author_role |
author |
author2 |
Biguetti, Claudia C. Simionato, Gustavo B. [UNESP] Custódio, Isabela C. Silva, Raquel B. P. [UNESP] Duarte, Marco A. H. Faverani, Leonardo P. [UNESP] Ervolino, Edilson [UNESP] Fakhouri, Walid D. Matsumoto, Mariza A. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Texas at Dallas Centro Universitário Sagrado Coração Universidade de São Paulo (USP) School of Dentistry |
dc.contributor.author.fl_str_mv |
Mahmoud, Ramez H. [UNESP] Biguetti, Claudia C. Simionato, Gustavo B. [UNESP] Custódio, Isabela C. Silva, Raquel B. P. [UNESP] Duarte, Marco A. H. Faverani, Leonardo P. [UNESP] Ervolino, Edilson [UNESP] Fakhouri, Walid D. Matsumoto, Mariza A. [UNESP] |
description |
This study investigated the role 5-lypoxigenase (5-LO) on alveolar socket healing in aged female mice treated with zoledronic acid (ZL). Forty 129/Sv female mice (64–68 weeks old), 20 wild type (WT) and 20 5-LO knockout (5LOKO) were equally distributed according to ZL treatment: WT Control, WT ZL, 5LOKO Control, and 5LOKO ZL. ZL groups were treated with an intraperitoneal injection of 250 µg/Kg of ZL, while controls were treated with saline. Treatments were administered once a week, starting four weeks before surgery for tooth extraction and until 7 and 21 days post-surgery. Mice were euthanized for a comprehensive microscopic analysis (microCT, histomorphometry and immunohistochemistry). WT ZL mice presented intense inflammatory infiltrate (7 days), delayed bone formation (21 days), reduced collagenous matrix quality, and a deficiency in Runx-2 + , TRAP + , and macrophages as compared to controls. 5LOKO ZL animals presented decreased number of Runx-2 + cells in comparison to 5LOKO Control at 7 days, but no major changes in bone healing as compared to WT or 5LOKO mice at 21 days. The knockout of 5LO favored intramembranous bone healing in aged female mice, with a direct impact on inflammatory response and bone metabolism on the development of ONJ-like lesions. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-01 2022-05-01T09:30:59Z 2022-05-01T09:30:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-021-98713-2 Scientific Reports, v. 11, n. 1, 2021. 2045-2322 http://hdl.handle.net/11449/233616 10.1038/s41598-021-98713-2 2-s2.0-85116365055 |
url |
http://dx.doi.org/10.1038/s41598-021-98713-2 http://hdl.handle.net/11449/233616 |
identifier_str_mv |
Scientific Reports, v. 11, n. 1, 2021. 2045-2322 10.1038/s41598-021-98713-2 2-s2.0-85116365055 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129115029504000 |