Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex

Detalhes bibliográficos
Autor(a) principal: Aleixo, Nadia Andrade
Data de Publicação: 2022
Outros Autores: Gomes, Pietra Stefany da Silva, Silva, Patrícia Bento da [UNESP], Sato, Mariana Rillo [UNESP], Campos, Débora Leite [UNESP], Barud, Hernane da Silva, Castro, Guillermo Raul, Islan, German Abel, Toledo, Constanza, Karp, Federico, Chorilli, Marlus [UNESP], Pavan, Fernando Rogério [UNESP], Resende, Flávia Aparecida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1080/02652048.2022.2025935
http://hdl.handle.net/11449/230267
Resumo: This study aimed to encapsulate and characterise a potential anti-tuberculosis copper complex (CuCl2(INH)2.H2O:I1) into polymeric nanoparticles (PNs) of polymethacrylate copolymers (Eudragit®, Eu) developed by nanoprecipitation method. NE30D, S100 and, E100 polymers were tested. The physicochemical characterisations were performed by DLS, TEM, FTIR, encapsulation efficiency and, in vitro release studies. Encapsulation of I1 in PN-NE30D, PN-E100, and PN-S100 was 26.3%, 94.5%, 22.6%, respectively. The particle size and zeta potentials were 82.3 nm and −24.5 mV for PNs-NE30D, 304.4 nm and +18.7 mV for PNs-E100, and 517.9 nm and −6.9 mV for PNs-S100, respectively. All PDIs were under 0.5. The formulations showed an I1 controlled release at alkaline pH with 29.7% from PNs-NE30D, 7.9% from PNs-E100 and, 28.1% from PNs-S100 at 1 h incubation. PNs were stable for at least 3 months. Particularly, PNs-NE30D demonstrated moderate inhibition of M. tuberculosis and low cytotoxic activity. None of the PNs induced mutagenicity.
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spelling Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complexCopper(II) complexesEudragit®mutagenicitypolymeric matricesresazurin assayThis study aimed to encapsulate and characterise a potential anti-tuberculosis copper complex (CuCl2(INH)2.H2O:I1) into polymeric nanoparticles (PNs) of polymethacrylate copolymers (Eudragit®, Eu) developed by nanoprecipitation method. NE30D, S100 and, E100 polymers were tested. The physicochemical characterisations were performed by DLS, TEM, FTIR, encapsulation efficiency and, in vitro release studies. Encapsulation of I1 in PN-NE30D, PN-E100, and PN-S100 was 26.3%, 94.5%, 22.6%, respectively. The particle size and zeta potentials were 82.3 nm and −24.5 mV for PNs-NE30D, 304.4 nm and +18.7 mV for PNs-E100, and 517.9 nm and −6.9 mV for PNs-S100, respectively. All PDIs were under 0.5. The formulations showed an I1 controlled release at alkaline pH with 29.7% from PNs-NE30D, 7.9% from PNs-E100 and, 28.1% from PNs-S100 at 1 h incubation. PNs were stable for at least 3 months. Particularly, PNs-NE30D demonstrated moderate inhibition of M. tuberculosis and low cytotoxic activity. None of the PNs induced mutagenicity.Department of Biological Sciences and Health University of Araraquara (UNIARA)Department of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State University (UNESP)Nanobiotechnology Laboratory Institute of Biological Sciences Department of Genetics and Morphology University of BrasiliaDepartment of Biological Sciences School of Pharmaceutical Sciences São Paulo State University (UNESP)Facultad de Ciencias Exactas Departmento de Química CINDEFI Laboratorio de Nanobiomateriales Universidad Nacional de La Plata–CONICET (CCT La Plata)Max Planck Laboratory for Structural Biology Chemistry and Molecular Biophysics of Rosario (MPLbioR UNR-MPIbpC) Partner Laboratory of the Max Planck Institute for Biophysical Chemistry (MPIbpC MPG) Universidad Nacional de Rosario Centro de Estudios Interdisciplinarios (CEI)Laboratorio de Química Fina (UNL-CONICET) Universidad Nacional del Litoral (UNL) INTECDepartment of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State University (UNESP)Department of Biological Sciences School of Pharmaceutical Sciences São Paulo State University (UNESP)University of Araraquara (UNIARA)Universidade Estadual Paulista (UNESP)University of BrasiliaUniversidad Nacional de La Plata–CONICET (CCT La Plata)Centro de Estudios Interdisciplinarios (CEI)INTECAleixo, Nadia AndradeGomes, Pietra Stefany da SilvaSilva, Patrícia Bento da [UNESP]Sato, Mariana Rillo [UNESP]Campos, Débora Leite [UNESP]Barud, Hernane da SilvaCastro, Guillermo RaulIslan, German AbelToledo, ConstanzaKarp, FedericoChorilli, Marlus [UNESP]Pavan, Fernando Rogério [UNESP]Resende, Flávia Aparecida2022-04-29T08:38:47Z2022-04-29T08:38:47Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article61-71http://dx.doi.org/10.1080/02652048.2022.2025935Journal of Microencapsulation, v. 39, n. 1, p. 61-71, 2022.1464-52460265-2048http://hdl.handle.net/11449/23026710.1080/02652048.2022.20259352-s2.0-85123395042Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Microencapsulationinfo:eu-repo/semantics/openAccess2024-06-24T13:45:50Zoai:repositorio.unesp.br:11449/230267Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:46:14.433329Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
title Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
spellingShingle Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
Aleixo, Nadia Andrade
Copper(II) complexes
Eudragit®
mutagenicity
polymeric matrices
resazurin assay
title_short Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
title_full Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
title_fullStr Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
title_full_unstemmed Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
title_sort Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
author Aleixo, Nadia Andrade
author_facet Aleixo, Nadia Andrade
Gomes, Pietra Stefany da Silva
Silva, Patrícia Bento da [UNESP]
Sato, Mariana Rillo [UNESP]
Campos, Débora Leite [UNESP]
Barud, Hernane da Silva
Castro, Guillermo Raul
Islan, German Abel
Toledo, Constanza
Karp, Federico
Chorilli, Marlus [UNESP]
Pavan, Fernando Rogério [UNESP]
Resende, Flávia Aparecida
author_role author
author2 Gomes, Pietra Stefany da Silva
Silva, Patrícia Bento da [UNESP]
Sato, Mariana Rillo [UNESP]
Campos, Débora Leite [UNESP]
Barud, Hernane da Silva
Castro, Guillermo Raul
Islan, German Abel
Toledo, Constanza
Karp, Federico
Chorilli, Marlus [UNESP]
Pavan, Fernando Rogério [UNESP]
Resende, Flávia Aparecida
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv University of Araraquara (UNIARA)
Universidade Estadual Paulista (UNESP)
University of Brasilia
Universidad Nacional de La Plata–CONICET (CCT La Plata)
Centro de Estudios Interdisciplinarios (CEI)
INTEC
dc.contributor.author.fl_str_mv Aleixo, Nadia Andrade
Gomes, Pietra Stefany da Silva
Silva, Patrícia Bento da [UNESP]
Sato, Mariana Rillo [UNESP]
Campos, Débora Leite [UNESP]
Barud, Hernane da Silva
Castro, Guillermo Raul
Islan, German Abel
Toledo, Constanza
Karp, Federico
Chorilli, Marlus [UNESP]
Pavan, Fernando Rogério [UNESP]
Resende, Flávia Aparecida
dc.subject.por.fl_str_mv Copper(II) complexes
Eudragit®
mutagenicity
polymeric matrices
resazurin assay
topic Copper(II) complexes
Eudragit®
mutagenicity
polymeric matrices
resazurin assay
description This study aimed to encapsulate and characterise a potential anti-tuberculosis copper complex (CuCl2(INH)2.H2O:I1) into polymeric nanoparticles (PNs) of polymethacrylate copolymers (Eudragit®, Eu) developed by nanoprecipitation method. NE30D, S100 and, E100 polymers were tested. The physicochemical characterisations were performed by DLS, TEM, FTIR, encapsulation efficiency and, in vitro release studies. Encapsulation of I1 in PN-NE30D, PN-E100, and PN-S100 was 26.3%, 94.5%, 22.6%, respectively. The particle size and zeta potentials were 82.3 nm and −24.5 mV for PNs-NE30D, 304.4 nm and +18.7 mV for PNs-E100, and 517.9 nm and −6.9 mV for PNs-S100, respectively. All PDIs were under 0.5. The formulations showed an I1 controlled release at alkaline pH with 29.7% from PNs-NE30D, 7.9% from PNs-E100 and, 28.1% from PNs-S100 at 1 h incubation. PNs were stable for at least 3 months. Particularly, PNs-NE30D demonstrated moderate inhibition of M. tuberculosis and low cytotoxic activity. None of the PNs induced mutagenicity.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:38:47Z
2022-04-29T08:38:47Z
2022-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/02652048.2022.2025935
Journal of Microencapsulation, v. 39, n. 1, p. 61-71, 2022.
1464-5246
0265-2048
http://hdl.handle.net/11449/230267
10.1080/02652048.2022.2025935
2-s2.0-85123395042
url http://dx.doi.org/10.1080/02652048.2022.2025935
http://hdl.handle.net/11449/230267
identifier_str_mv Journal of Microencapsulation, v. 39, n. 1, p. 61-71, 2022.
1464-5246
0265-2048
10.1080/02652048.2022.2025935
2-s2.0-85123395042
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Microencapsulation
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 61-71
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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