Cerebellar Insulin/IGF-1 signaling in diabetic rats: Effects of exercise training
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.neulet.2016.12.059 http://hdl.handle.net/11449/178560 |
Resumo: | The Diabetes Mellitus (DM) is a chronic disease associated with loss of brain regions such as the cerebellum, increasing the risk of developing neurodegenerative diseases such as Parkinson's disease (PD). In the brain of diabetic and PD organisms the insulin/IGF-1 signaling is altered. Exercise training is an effective intervention for the prevention of neurodegerative diseases since it release neurotrophic factors and regulating insulin/IGF-1 signaling in the brain. This study aimed to evaluate the proteins involved in the insulin/IGF-1 pathway in the cerebellum of diabetic rats subjected to exercise training protocol. Wistar rats were distributed in four groups: sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). Diabetes was induced by Alloxan (ALX) (32 mg/kg b.w.). The training program consisted in swimming 5 days/week, 1 h/day, during 6 weeks, supporting an overload corresponding to 90% of the anaerobic threshold. At the end, cerebellum was extracted to determinate the protein expression of GSK-3β, IRβ and IGF-1R and the phosphorylation of β-amyloid, Tau, ERK1 + ERK2 by Western Blot analysis. All dependent variables were analyzed by one-way analysis of variance with significance level of 5%. Diabetes causes hyperglycemia in both diabetic groups; however, in TD, there was a reduction in hyperglycemia compared to SD. Diabetes increased Tau and β-amyloid phosphorylation in both SD and TD groups. Furthermore, aerobic exercise increased ERK1 + ERK2 expression in TC. The data showed that in cerebellum of diabetic rats induced by alloxan there are some proteins expression like Parkinson cerebellum increased, and the exercise training was not able to modulate the expression of these proteins. |
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Cerebellar Insulin/IGF-1 signaling in diabetic rats: Effects of exercise trainingCerebellumDiabetesExerciseThe Diabetes Mellitus (DM) is a chronic disease associated with loss of brain regions such as the cerebellum, increasing the risk of developing neurodegenerative diseases such as Parkinson's disease (PD). In the brain of diabetic and PD organisms the insulin/IGF-1 signaling is altered. Exercise training is an effective intervention for the prevention of neurodegerative diseases since it release neurotrophic factors and regulating insulin/IGF-1 signaling in the brain. This study aimed to evaluate the proteins involved in the insulin/IGF-1 pathway in the cerebellum of diabetic rats subjected to exercise training protocol. Wistar rats were distributed in four groups: sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). Diabetes was induced by Alloxan (ALX) (32 mg/kg b.w.). The training program consisted in swimming 5 days/week, 1 h/day, during 6 weeks, supporting an overload corresponding to 90% of the anaerobic threshold. At the end, cerebellum was extracted to determinate the protein expression of GSK-3β, IRβ and IGF-1R and the phosphorylation of β-amyloid, Tau, ERK1 + ERK2 by Western Blot analysis. All dependent variables were analyzed by one-way analysis of variance with significance level of 5%. Diabetes causes hyperglycemia in both diabetic groups; however, in TD, there was a reduction in hyperglycemia compared to SD. Diabetes increased Tau and β-amyloid phosphorylation in both SD and TD groups. Furthermore, aerobic exercise increased ERK1 + ERK2 expression in TC. The data showed that in cerebellum of diabetic rats induced by alloxan there are some proteins expression like Parkinson cerebellum increased, and the exercise training was not able to modulate the expression of these proteins.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biosciences São Paulo Federal University (UNIFESP)Sport Science Course University of Campinas UNICAMPDepartment of Physical Education São Paulo State University (UNESP)Department of Physical Education Catholic University Center UnisalesianoDepartament of Physical Education University Center of Patos de MinasDepartment of Physical Education São Paulo State University (UNESP)CNPq: 142587/2007-9FAPESP: 2010/18257-0Universidade Federal de São Paulo (UNIFESP)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Catholic University Center UnisalesianoUniversity Center of Patos de MinasBorges, Mariana EirasRibeiro, Alessandra MussiPauli, José RodrigoArantes, Luciana MendonçaLuciano, Eliete [UNESP]de Moura, Leandro Pereirade Almeida Leme, José Alexandre CuriacosMedeiros, AlessandraBertolini, Natália Oliveira [UNESP]Sibuya, Clarice Yoshiko [UNESP]Gomes, Ricardo José2018-12-11T17:30:55Z2018-12-11T17:30:55Z2017-02-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article157-161application/pdfhttp://dx.doi.org/10.1016/j.neulet.2016.12.059Neuroscience Letters, v. 639, p. 157-161.1872-79720304-3940http://hdl.handle.net/11449/17856010.1016/j.neulet.2016.12.0592-s2.0-850086588892-s2.0-85008658889.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeuroscience Letters0,946info:eu-repo/semantics/openAccess2023-11-01T06:15:19Zoai:repositorio.unesp.br:11449/178560Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:40:06.000779Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cerebellar Insulin/IGF-1 signaling in diabetic rats: Effects of exercise training |
title |
Cerebellar Insulin/IGF-1 signaling in diabetic rats: Effects of exercise training |
spellingShingle |
Cerebellar Insulin/IGF-1 signaling in diabetic rats: Effects of exercise training Borges, Mariana Eiras Cerebellum Diabetes Exercise |
title_short |
Cerebellar Insulin/IGF-1 signaling in diabetic rats: Effects of exercise training |
title_full |
Cerebellar Insulin/IGF-1 signaling in diabetic rats: Effects of exercise training |
title_fullStr |
Cerebellar Insulin/IGF-1 signaling in diabetic rats: Effects of exercise training |
title_full_unstemmed |
Cerebellar Insulin/IGF-1 signaling in diabetic rats: Effects of exercise training |
title_sort |
Cerebellar Insulin/IGF-1 signaling in diabetic rats: Effects of exercise training |
author |
Borges, Mariana Eiras |
author_facet |
Borges, Mariana Eiras Ribeiro, Alessandra Mussi Pauli, José Rodrigo Arantes, Luciana Mendonça Luciano, Eliete [UNESP] de Moura, Leandro Pereira de Almeida Leme, José Alexandre Curiacos Medeiros, Alessandra Bertolini, Natália Oliveira [UNESP] Sibuya, Clarice Yoshiko [UNESP] Gomes, Ricardo José |
author_role |
author |
author2 |
Ribeiro, Alessandra Mussi Pauli, José Rodrigo Arantes, Luciana Mendonça Luciano, Eliete [UNESP] de Moura, Leandro Pereira de Almeida Leme, José Alexandre Curiacos Medeiros, Alessandra Bertolini, Natália Oliveira [UNESP] Sibuya, Clarice Yoshiko [UNESP] Gomes, Ricardo José |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) Catholic University Center Unisalesiano University Center of Patos de Minas |
dc.contributor.author.fl_str_mv |
Borges, Mariana Eiras Ribeiro, Alessandra Mussi Pauli, José Rodrigo Arantes, Luciana Mendonça Luciano, Eliete [UNESP] de Moura, Leandro Pereira de Almeida Leme, José Alexandre Curiacos Medeiros, Alessandra Bertolini, Natália Oliveira [UNESP] Sibuya, Clarice Yoshiko [UNESP] Gomes, Ricardo José |
dc.subject.por.fl_str_mv |
Cerebellum Diabetes Exercise |
topic |
Cerebellum Diabetes Exercise |
description |
The Diabetes Mellitus (DM) is a chronic disease associated with loss of brain regions such as the cerebellum, increasing the risk of developing neurodegenerative diseases such as Parkinson's disease (PD). In the brain of diabetic and PD organisms the insulin/IGF-1 signaling is altered. Exercise training is an effective intervention for the prevention of neurodegerative diseases since it release neurotrophic factors and regulating insulin/IGF-1 signaling in the brain. This study aimed to evaluate the proteins involved in the insulin/IGF-1 pathway in the cerebellum of diabetic rats subjected to exercise training protocol. Wistar rats were distributed in four groups: sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). Diabetes was induced by Alloxan (ALX) (32 mg/kg b.w.). The training program consisted in swimming 5 days/week, 1 h/day, during 6 weeks, supporting an overload corresponding to 90% of the anaerobic threshold. At the end, cerebellum was extracted to determinate the protein expression of GSK-3β, IRβ and IGF-1R and the phosphorylation of β-amyloid, Tau, ERK1 + ERK2 by Western Blot analysis. All dependent variables were analyzed by one-way analysis of variance with significance level of 5%. Diabetes causes hyperglycemia in both diabetic groups; however, in TD, there was a reduction in hyperglycemia compared to SD. Diabetes increased Tau and β-amyloid phosphorylation in both SD and TD groups. Furthermore, aerobic exercise increased ERK1 + ERK2 expression in TC. The data showed that in cerebellum of diabetic rats induced by alloxan there are some proteins expression like Parkinson cerebellum increased, and the exercise training was not able to modulate the expression of these proteins. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-03 2018-12-11T17:30:55Z 2018-12-11T17:30:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.neulet.2016.12.059 Neuroscience Letters, v. 639, p. 157-161. 1872-7972 0304-3940 http://hdl.handle.net/11449/178560 10.1016/j.neulet.2016.12.059 2-s2.0-85008658889 2-s2.0-85008658889.pdf |
url |
http://dx.doi.org/10.1016/j.neulet.2016.12.059 http://hdl.handle.net/11449/178560 |
identifier_str_mv |
Neuroscience Letters, v. 639, p. 157-161. 1872-7972 0304-3940 10.1016/j.neulet.2016.12.059 2-s2.0-85008658889 2-s2.0-85008658889.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Neuroscience Letters 0,946 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
157-161 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128685350322176 |