Effects of physiological levels of daidzein on cell proliferation of tumoral and non-tumoral breast cells lines
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.oapublishinglondon.com/article/1296 http://hdl.handle.net/11449/141434 |
Resumo: | Introduction: Soy isoflavones are associated with the lower breast cancer risk found in Asiatic populations. They can interfere in various stages of the carcinogenesis process, preventing the development of this pathology and, for this reason, its consumption became popular. Instead of the beneficial effects found in literature, most chemopreventive in vitro studies use non-physiological levels of daidzein (> 30 µM). In our study, the effects of maximal physiological levels of daidzein was investigated in two human breast cell lines: HB4a (non-tumoral) and MCF-7 (tumoral). Experiments to verify cytotoxicity, induction of cell death by apoptosis and, additionally, cell cycle arrest were performed. Materials and methods: Cytotoxic effect of daidzein (0.1 - 100 µM) was assessed using resazurinbased assay. Flow cytometry tests were performed to investigate apoptosis induction (annexin VFITC/PI) and cell cycle arrest (propidium iodide staining of DNA content) by daidzein at 10 and 25 µM. Changes in expression of apoptosis related genes after treatment of cells with daidzein (10 and 25 µM) were investigated using quantitative real time PCR. Results: Daidzein ranging from 0.1 to 100 µM (for 24 and 48h) were not cytotoxic to both cell lines. Concentrations of 10 and 25 µM did not induce apoptosis in these cells, instead of statistical significant changes in gene expression of some of the target genes (CASP-3, CASP-7, BAX, BCL-xL, COX-2). Both concentrations of daidzein arrested cell cycle in G0/G1 phase only in MCF- 7. Conclusion: Daidzein at maximal achievable physiologic serum levels selectively arrested cell cycle only in the tumoral MCF-7, and did not induce cell death by apoptosis, one of the major effects described in literature. We hope that our study helps in understanding the chemopreventive effects of low levels of soy isoflavones in breast carcinogenesis. |
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Effects of physiological levels of daidzein on cell proliferation of tumoral and non-tumoral breast cells linesIntroduction: Soy isoflavones are associated with the lower breast cancer risk found in Asiatic populations. They can interfere in various stages of the carcinogenesis process, preventing the development of this pathology and, for this reason, its consumption became popular. Instead of the beneficial effects found in literature, most chemopreventive in vitro studies use non-physiological levels of daidzein (> 30 µM). In our study, the effects of maximal physiological levels of daidzein was investigated in two human breast cell lines: HB4a (non-tumoral) and MCF-7 (tumoral). Experiments to verify cytotoxicity, induction of cell death by apoptosis and, additionally, cell cycle arrest were performed. Materials and methods: Cytotoxic effect of daidzein (0.1 - 100 µM) was assessed using resazurinbased assay. Flow cytometry tests were performed to investigate apoptosis induction (annexin VFITC/PI) and cell cycle arrest (propidium iodide staining of DNA content) by daidzein at 10 and 25 µM. Changes in expression of apoptosis related genes after treatment of cells with daidzein (10 and 25 µM) were investigated using quantitative real time PCR. Results: Daidzein ranging from 0.1 to 100 µM (for 24 and 48h) were not cytotoxic to both cell lines. Concentrations of 10 and 25 µM did not induce apoptosis in these cells, instead of statistical significant changes in gene expression of some of the target genes (CASP-3, CASP-7, BAX, BCL-xL, COX-2). Both concentrations of daidzein arrested cell cycle in G0/G1 phase only in MCF- 7. Conclusion: Daidzein at maximal achievable physiologic serum levels selectively arrested cell cycle only in the tumoral MCF-7, and did not induce cell death by apoptosis, one of the major effects described in literature. We hope that our study helps in understanding the chemopreventive effects of low levels of soy isoflavones in breast carcinogenesis.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade de São Paulo, Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão PretoUniversidade de São Paulo, Departamento de Clínica Médica, Faculdade de Medicina de Ribeirão PretoUniversidade Estadual de Londrina, Departamento de PatologiaUniversidade Estadual de Londrina, Departamento de BiologiaUniversidade Estadual Paulista, Instituto de Biociências de Rio ClaroCNPq: 471938/2008-4Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Universidade Estadual de Londrina (UEL)Tsuboy, Marcela Stefanini Ferreira [UNESP]Marcarini, Juliana Cristina [UNESP]Souza, Alecsandra Oliveira dePaula, Natalia Aparecida deLuiz, Rodrigo CabralDorta, Daniel JunqueiraMantovani, Mário SérgioRibeiro, Lucia Regina [UNESP]2016-07-07T12:38:18Z2016-07-07T12:38:18Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-9application/pdfhttp://www.oapublishinglondon.com/article/1296OA Cancer, v. 2, n. 1, p. 1-9, 2014.2053-3918http://hdl.handle.net/11449/141434ISSN2053-3918-2014-02-01-01-09.pdf7787314478210146796534287701605871507642858871921779939619083787663097092023663663391055416345389570878174185086Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOA Cancerinfo:eu-repo/semantics/openAccess2023-11-14T06:10:56Zoai:repositorio.unesp.br:11449/141434Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-14T06:10:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of physiological levels of daidzein on cell proliferation of tumoral and non-tumoral breast cells lines |
title |
Effects of physiological levels of daidzein on cell proliferation of tumoral and non-tumoral breast cells lines |
spellingShingle |
Effects of physiological levels of daidzein on cell proliferation of tumoral and non-tumoral breast cells lines Tsuboy, Marcela Stefanini Ferreira [UNESP] |
title_short |
Effects of physiological levels of daidzein on cell proliferation of tumoral and non-tumoral breast cells lines |
title_full |
Effects of physiological levels of daidzein on cell proliferation of tumoral and non-tumoral breast cells lines |
title_fullStr |
Effects of physiological levels of daidzein on cell proliferation of tumoral and non-tumoral breast cells lines |
title_full_unstemmed |
Effects of physiological levels of daidzein on cell proliferation of tumoral and non-tumoral breast cells lines |
title_sort |
Effects of physiological levels of daidzein on cell proliferation of tumoral and non-tumoral breast cells lines |
author |
Tsuboy, Marcela Stefanini Ferreira [UNESP] |
author_facet |
Tsuboy, Marcela Stefanini Ferreira [UNESP] Marcarini, Juliana Cristina [UNESP] Souza, Alecsandra Oliveira de Paula, Natalia Aparecida de Luiz, Rodrigo Cabral Dorta, Daniel Junqueira Mantovani, Mário Sérgio Ribeiro, Lucia Regina [UNESP] |
author_role |
author |
author2 |
Marcarini, Juliana Cristina [UNESP] Souza, Alecsandra Oliveira de Paula, Natalia Aparecida de Luiz, Rodrigo Cabral Dorta, Daniel Junqueira Mantovani, Mário Sérgio Ribeiro, Lucia Regina [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Universidade Estadual de Londrina (UEL) |
dc.contributor.author.fl_str_mv |
Tsuboy, Marcela Stefanini Ferreira [UNESP] Marcarini, Juliana Cristina [UNESP] Souza, Alecsandra Oliveira de Paula, Natalia Aparecida de Luiz, Rodrigo Cabral Dorta, Daniel Junqueira Mantovani, Mário Sérgio Ribeiro, Lucia Regina [UNESP] |
description |
Introduction: Soy isoflavones are associated with the lower breast cancer risk found in Asiatic populations. They can interfere in various stages of the carcinogenesis process, preventing the development of this pathology and, for this reason, its consumption became popular. Instead of the beneficial effects found in literature, most chemopreventive in vitro studies use non-physiological levels of daidzein (> 30 µM). In our study, the effects of maximal physiological levels of daidzein was investigated in two human breast cell lines: HB4a (non-tumoral) and MCF-7 (tumoral). Experiments to verify cytotoxicity, induction of cell death by apoptosis and, additionally, cell cycle arrest were performed. Materials and methods: Cytotoxic effect of daidzein (0.1 - 100 µM) was assessed using resazurinbased assay. Flow cytometry tests were performed to investigate apoptosis induction (annexin VFITC/PI) and cell cycle arrest (propidium iodide staining of DNA content) by daidzein at 10 and 25 µM. Changes in expression of apoptosis related genes after treatment of cells with daidzein (10 and 25 µM) were investigated using quantitative real time PCR. Results: Daidzein ranging from 0.1 to 100 µM (for 24 and 48h) were not cytotoxic to both cell lines. Concentrations of 10 and 25 µM did not induce apoptosis in these cells, instead of statistical significant changes in gene expression of some of the target genes (CASP-3, CASP-7, BAX, BCL-xL, COX-2). Both concentrations of daidzein arrested cell cycle in G0/G1 phase only in MCF- 7. Conclusion: Daidzein at maximal achievable physiologic serum levels selectively arrested cell cycle only in the tumoral MCF-7, and did not induce cell death by apoptosis, one of the major effects described in literature. We hope that our study helps in understanding the chemopreventive effects of low levels of soy isoflavones in breast carcinogenesis. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2016-07-07T12:38:18Z 2016-07-07T12:38:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.oapublishinglondon.com/article/1296 OA Cancer, v. 2, n. 1, p. 1-9, 2014. 2053-3918 http://hdl.handle.net/11449/141434 ISSN2053-3918-2014-02-01-01-09.pdf 7787314478210146 7965342877016058 7150764285887192 1779939619083787 6630970920236636 6339105541634538 9570878174185086 |
url |
http://www.oapublishinglondon.com/article/1296 http://hdl.handle.net/11449/141434 |
identifier_str_mv |
OA Cancer, v. 2, n. 1, p. 1-9, 2014. 2053-3918 ISSN2053-3918-2014-02-01-01-09.pdf 7787314478210146 7965342877016058 7150764285887192 1779939619083787 6630970920236636 6339105541634538 9570878174185086 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
OA Cancer |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-9 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046474516267008 |