Granulomatous reaction in the hamster cheek pouch induced by killed Paracoccidioides brasiliensis.

Detalhes bibliográficos
Autor(a) principal: Arruda, M. S. [UNESP]
Data de Publicação: 1995
Outros Autores: Montenegro, M. R. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/219182
Resumo: We have studied the role of Paracoccidioides brasiliensis viability in the morphology of paracoccidioidomycotic granulomas in the hamster cheek pouch, an immunologically privileged site. Naive (N = 75) and previously sensitized (N = 50) two-month old male hamsters (Mesocricetus auratus) were inoculated into the pouch with 5 x 10(5) live or heat- or formalin-killed fungi. Previously sensitized animals presented a positive footpad test and immunodiffusion demonstrable antibodies (titer 1/32), at the time of sacrifice; naive animals were always negative for those immunological tests. The histological results showed that, like viable P. brasiliensis, killed fungi evoke typical epithelioid granulomas in 100% of animals, even in the absence of immunodiffusion or footpad test detectable immune response. The granulomas elicited by killed fungi were devoid of giant cells or a mononuclear cell halo, suggesting that live proliferating fungi or their products may be involved in these events.
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spelling Granulomatous reaction in the hamster cheek pouch induced by killed Paracoccidioides brasiliensis.We have studied the role of Paracoccidioides brasiliensis viability in the morphology of paracoccidioidomycotic granulomas in the hamster cheek pouch, an immunologically privileged site. Naive (N = 75) and previously sensitized (N = 50) two-month old male hamsters (Mesocricetus auratus) were inoculated into the pouch with 5 x 10(5) live or heat- or formalin-killed fungi. Previously sensitized animals presented a positive footpad test and immunodiffusion demonstrable antibodies (titer 1/32), at the time of sacrifice; naive animals were always negative for those immunological tests. The histological results showed that, like viable P. brasiliensis, killed fungi evoke typical epithelioid granulomas in 100% of animals, even in the absence of immunodiffusion or footpad test detectable immune response. The granulomas elicited by killed fungi were devoid of giant cells or a mononuclear cell halo, suggesting that live proliferating fungi or their products may be involved in these events.Departamento de Ciências Biológicas Faculdade de Ciências Universidade Estadual PaulistaDepartamento de Ciências Biológicas Faculdade de Ciências Universidade Estadual PaulistaUniversidade Estadual Paulista (UNESP)Arruda, M. S. [UNESP]Montenegro, M. R. [UNESP]2022-04-28T18:54:08Z2022-04-28T18:54:08Z1995-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article209-212Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], v. 28, n. 2, p. 209-212, 1995.0100-879Xhttp://hdl.handle.net/11449/2191822-s2.0-0029242136Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]info:eu-repo/semantics/openAccess2022-04-28T18:54:08Zoai:repositorio.unesp.br:11449/219182Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:13:35.883072Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Granulomatous reaction in the hamster cheek pouch induced by killed Paracoccidioides brasiliensis.
title Granulomatous reaction in the hamster cheek pouch induced by killed Paracoccidioides brasiliensis.
spellingShingle Granulomatous reaction in the hamster cheek pouch induced by killed Paracoccidioides brasiliensis.
Arruda, M. S. [UNESP]
title_short Granulomatous reaction in the hamster cheek pouch induced by killed Paracoccidioides brasiliensis.
title_full Granulomatous reaction in the hamster cheek pouch induced by killed Paracoccidioides brasiliensis.
title_fullStr Granulomatous reaction in the hamster cheek pouch induced by killed Paracoccidioides brasiliensis.
title_full_unstemmed Granulomatous reaction in the hamster cheek pouch induced by killed Paracoccidioides brasiliensis.
title_sort Granulomatous reaction in the hamster cheek pouch induced by killed Paracoccidioides brasiliensis.
author Arruda, M. S. [UNESP]
author_facet Arruda, M. S. [UNESP]
Montenegro, M. R. [UNESP]
author_role author
author2 Montenegro, M. R. [UNESP]
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Arruda, M. S. [UNESP]
Montenegro, M. R. [UNESP]
description We have studied the role of Paracoccidioides brasiliensis viability in the morphology of paracoccidioidomycotic granulomas in the hamster cheek pouch, an immunologically privileged site. Naive (N = 75) and previously sensitized (N = 50) two-month old male hamsters (Mesocricetus auratus) were inoculated into the pouch with 5 x 10(5) live or heat- or formalin-killed fungi. Previously sensitized animals presented a positive footpad test and immunodiffusion demonstrable antibodies (titer 1/32), at the time of sacrifice; naive animals were always negative for those immunological tests. The histological results showed that, like viable P. brasiliensis, killed fungi evoke typical epithelioid granulomas in 100% of animals, even in the absence of immunodiffusion or footpad test detectable immune response. The granulomas elicited by killed fungi were devoid of giant cells or a mononuclear cell halo, suggesting that live proliferating fungi or their products may be involved in these events.
publishDate 1995
dc.date.none.fl_str_mv 1995-01-01
2022-04-28T18:54:08Z
2022-04-28T18:54:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], v. 28, n. 2, p. 209-212, 1995.
0100-879X
http://hdl.handle.net/11449/219182
2-s2.0-0029242136
identifier_str_mv Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], v. 28, n. 2, p. 209-212, 1995.
0100-879X
2-s2.0-0029242136
url http://hdl.handle.net/11449/219182
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 209-212
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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