Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosa
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1472-6882-9-15 http://hdl.handle.net/11449/26333 |
Resumo: | Background: Angiogenesis, a complex multistep process that comprehends proliferation, migration and anastomosis of endothelial cells (EC), has a major role in the development of pathologic conditions such as inflammatory diseases, tumor growth and metastasis. Brazilian flora, the most diverse in the world, is an interesting spot to prospect for new chemical leads, being an important source of new anticancer drugs. Plant-derived alkaloids have traditionally been of interest due to their pronounced physiological activities. We investigated the anti-angiogenic potential of the naturally occurring guanidine alkaloid pterogynidine (Pt) isolated from the Brazilian plant Alchornea glandulosa. The purpose of this study was to examine which features of the angiogenic process could be disturbed by Pt.Methods: Human umbilical vein endothelial cells (HUVEC) were incubated with 8 mu M Pt and cell viability, proliferation, apoptosis, invasion and capillary-like structures formation were addressed. Nuclear factor kappa B (NF kappa B), a transcription factor implicated in these processes, was also evaluated in HUVEC incubated with Pt. Quantifications were expressed as mean +/- SD of five independent experiments and one-way analysis of variance (ANOVA) followed by the Dunnet test was used.Results: A significant decrease in proliferation and invasion capacity and an effective increase in apoptosis as assessed by bromodeoxyuridine (BrdU), double-chamber and terminal transferase dUTP nick end labeling (TUNEL) assay, respectively, have been found. Pt also led to a drastic reduction in the number of capillary-like structures formation when HUVEC were cultured on growth factor reduced-Matrigel (GFR-Matrigel) coated plates. In addition, incubation of HUVEC with Pt resulted in reduced NF kappa B activity.Conclusion: These findings emphasize the potential use of Pt against pathological situations where angiogenesis is stimulated as tumor development. |
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Repositório Institucional da UNESP |
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Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosaBackground: Angiogenesis, a complex multistep process that comprehends proliferation, migration and anastomosis of endothelial cells (EC), has a major role in the development of pathologic conditions such as inflammatory diseases, tumor growth and metastasis. Brazilian flora, the most diverse in the world, is an interesting spot to prospect for new chemical leads, being an important source of new anticancer drugs. Plant-derived alkaloids have traditionally been of interest due to their pronounced physiological activities. We investigated the anti-angiogenic potential of the naturally occurring guanidine alkaloid pterogynidine (Pt) isolated from the Brazilian plant Alchornea glandulosa. The purpose of this study was to examine which features of the angiogenic process could be disturbed by Pt.Methods: Human umbilical vein endothelial cells (HUVEC) were incubated with 8 mu M Pt and cell viability, proliferation, apoptosis, invasion and capillary-like structures formation were addressed. Nuclear factor kappa B (NF kappa B), a transcription factor implicated in these processes, was also evaluated in HUVEC incubated with Pt. Quantifications were expressed as mean +/- SD of five independent experiments and one-way analysis of variance (ANOVA) followed by the Dunnet test was used.Results: A significant decrease in proliferation and invasion capacity and an effective increase in apoptosis as assessed by bromodeoxyuridine (BrdU), double-chamber and terminal transferase dUTP nick end labeling (TUNEL) assay, respectively, have been found. Pt also led to a drastic reduction in the number of capillary-like structures formation when HUVEC were cultured on growth factor reduced-Matrigel (GFR-Matrigel) coated plates. In addition, incubation of HUVEC with Pt resulted in reduced NF kappa B activity.Conclusion: These findings emphasize the potential use of Pt against pathological situations where angiogenesis is stimulated as tumor development.FCTCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)ERAB, European Advisory BoardFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State Univ, UNESP, Dept Clin Anal, Fac Pharmaceut Sci Araraquara, BR-14801902 São Paulo, BrazilUniv Porto, Fac Med, Dept Biochem FCT U38, Oporto, PortugalSão Paulo State Univ, UNESP, Dept Organ Chem, Araraquara Inst Chem, BR-14801902 São Paulo, BrazilSão Paulo State Univ, UNESP, Dept Clin Anal, Fac Pharmaceut Sci Araraquara, BR-14801902 São Paulo, BrazilSão Paulo State Univ, UNESP, Dept Organ Chem, Araraquara Inst Chem, BR-14801902 São Paulo, BrazilCAPES: 1008/07-2ERAB, European Advisory Board: EA0641FAPESP: 03/02176-7Biomed Central Ltd.Universidade Estadual Paulista (Unesp)Univ PortoLopes, Flavia C. M. [UNESP]Rocha, AnaPirraco, AnaRegasini, Luis O. [UNESP]Silva, Dulce Helena Siqueira [UNESP]Bolzani, Vanderlan da Silva [UNESP]Azevedo, IsabelCarlos, Iracilda Z. [UNESP]Soares, Raquel2014-05-20T14:21:11Z2014-05-20T14:21:11Z2009-05-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://dx.doi.org/10.1186/1472-6882-9-15Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 9, p. 11, 2009.1472-6882http://hdl.handle.net/11449/2633310.1186/1472-6882-9-15WOS:000267598400001WOS000267598400001.pdf470200490423124844840836852516730000-0002-1516-7765Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Complementary and Alternative Medicine2.1090,858info:eu-repo/semantics/openAccess2024-01-04T06:30:19Zoai:repositorio.unesp.br:11449/26333Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-04T06:30:19Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosa |
title |
Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosa |
spellingShingle |
Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosa Lopes, Flavia C. M. [UNESP] |
title_short |
Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosa |
title_full |
Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosa |
title_fullStr |
Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosa |
title_full_unstemmed |
Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosa |
title_sort |
Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosa |
author |
Lopes, Flavia C. M. [UNESP] |
author_facet |
Lopes, Flavia C. M. [UNESP] Rocha, Ana Pirraco, Ana Regasini, Luis O. [UNESP] Silva, Dulce Helena Siqueira [UNESP] Bolzani, Vanderlan da Silva [UNESP] Azevedo, Isabel Carlos, Iracilda Z. [UNESP] Soares, Raquel |
author_role |
author |
author2 |
Rocha, Ana Pirraco, Ana Regasini, Luis O. [UNESP] Silva, Dulce Helena Siqueira [UNESP] Bolzani, Vanderlan da Silva [UNESP] Azevedo, Isabel Carlos, Iracilda Z. [UNESP] Soares, Raquel |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Porto |
dc.contributor.author.fl_str_mv |
Lopes, Flavia C. M. [UNESP] Rocha, Ana Pirraco, Ana Regasini, Luis O. [UNESP] Silva, Dulce Helena Siqueira [UNESP] Bolzani, Vanderlan da Silva [UNESP] Azevedo, Isabel Carlos, Iracilda Z. [UNESP] Soares, Raquel |
description |
Background: Angiogenesis, a complex multistep process that comprehends proliferation, migration and anastomosis of endothelial cells (EC), has a major role in the development of pathologic conditions such as inflammatory diseases, tumor growth and metastasis. Brazilian flora, the most diverse in the world, is an interesting spot to prospect for new chemical leads, being an important source of new anticancer drugs. Plant-derived alkaloids have traditionally been of interest due to their pronounced physiological activities. We investigated the anti-angiogenic potential of the naturally occurring guanidine alkaloid pterogynidine (Pt) isolated from the Brazilian plant Alchornea glandulosa. The purpose of this study was to examine which features of the angiogenic process could be disturbed by Pt.Methods: Human umbilical vein endothelial cells (HUVEC) were incubated with 8 mu M Pt and cell viability, proliferation, apoptosis, invasion and capillary-like structures formation were addressed. Nuclear factor kappa B (NF kappa B), a transcription factor implicated in these processes, was also evaluated in HUVEC incubated with Pt. Quantifications were expressed as mean +/- SD of five independent experiments and one-way analysis of variance (ANOVA) followed by the Dunnet test was used.Results: A significant decrease in proliferation and invasion capacity and an effective increase in apoptosis as assessed by bromodeoxyuridine (BrdU), double-chamber and terminal transferase dUTP nick end labeling (TUNEL) assay, respectively, have been found. Pt also led to a drastic reduction in the number of capillary-like structures formation when HUVEC were cultured on growth factor reduced-Matrigel (GFR-Matrigel) coated plates. In addition, incubation of HUVEC with Pt resulted in reduced NF kappa B activity.Conclusion: These findings emphasize the potential use of Pt against pathological situations where angiogenesis is stimulated as tumor development. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-05-22 2014-05-20T14:21:11Z 2014-05-20T14:21:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1472-6882-9-15 Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 9, p. 11, 2009. 1472-6882 http://hdl.handle.net/11449/26333 10.1186/1472-6882-9-15 WOS:000267598400001 WOS000267598400001.pdf 4702004904231248 4484083685251673 0000-0002-1516-7765 |
url |
http://dx.doi.org/10.1186/1472-6882-9-15 http://hdl.handle.net/11449/26333 |
identifier_str_mv |
Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 9, p. 11, 2009. 1472-6882 10.1186/1472-6882-9-15 WOS:000267598400001 WOS000267598400001.pdf 4702004904231248 4484083685251673 0000-0002-1516-7765 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Complementary and Alternative Medicine 2.109 0,858 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965518846754816 |