Genetic analysis of Giardia duodenalis isolates from children of low-income families living in an economically successful region in Southeastern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-9946202062020 http://hdl.handle.net/11449/196746 |
Resumo: | Giardia duodenalis is one of the most important and widespread gastrointestinal parasites in the world. Despite its relevance as a causative agent of diarrhea, asymptomatic giardiasis occurs frequently. especially in low resources settings in which children are exposed to many risk factors. Based on microscopic examination and the polymerase chain reaction (PCR) amplification and sequencing of beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes, we assessed G. duodenalis occurrence and genetic diversity in isolates of children attending a daycare center and living in low income families, in an economically successful region. Considering both, microscopic examination and PCR/sequencing methods, the overall prevalence of Giardia infection was 51.4%, with the highest frequency in children aged 1-4 years old (p<0.05). Genotyping of 50 isolates revealed that the assemblage A was found in 60% of the samples (30/50), followed by the assemblage B in 38% (19/50) and 2% of mixed-assemblage infections (1/50). At the sub-assemblage level, isolates genotyped as A were All and among isolates B, BIII and BIV were identified. Both assemblages A and B were detected in children of all age groups . however assemblage A was more prevalent. The detection of anthroponotic assemblages and sub-assemblages (AII, BM and BIV) reinforces human-to-human transmission, mainly in children of all age groups when they have not yet received toilet training, making them more vulnerable to infection. |
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Genetic analysis of Giardia duodenalis isolates from children of low-income families living in an economically successful region in Southeastern BrazilGiardia duodenalisGenetic diversityChildrenDaycareLow incomeGiardia duodenalis is one of the most important and widespread gastrointestinal parasites in the world. Despite its relevance as a causative agent of diarrhea, asymptomatic giardiasis occurs frequently. especially in low resources settings in which children are exposed to many risk factors. Based on microscopic examination and the polymerase chain reaction (PCR) amplification and sequencing of beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes, we assessed G. duodenalis occurrence and genetic diversity in isolates of children attending a daycare center and living in low income families, in an economically successful region. Considering both, microscopic examination and PCR/sequencing methods, the overall prevalence of Giardia infection was 51.4%, with the highest frequency in children aged 1-4 years old (p<0.05). Genotyping of 50 isolates revealed that the assemblage A was found in 60% of the samples (30/50), followed by the assemblage B in 38% (19/50) and 2% of mixed-assemblage infections (1/50). At the sub-assemblage level, isolates genotyped as A were All and among isolates B, BIII and BIV were identified. Both assemblages A and B were detected in children of all age groups . however assemblage A was more prevalent. The detection of anthroponotic assemblages and sub-assemblages (AII, BM and BIV) reinforces human-to-human transmission, mainly in children of all age groups when they have not yet received toilet training, making them more vulnerable to infection.Ctr Paula Souza, ETEC Darcy Pereira Moraes, Itapetininga, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Parasitol, Dist Rubiao Jr S-N,Caixa Postal 510, BR-18618970 Botucatu, SP, BrazilFac Integradas Bauru, Bauru, SP, BrazilUniv Sagrad Coracao, Dept Ciencias Saude, Bauru, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Parasitol, Dist Rubiao Jr S-N,Caixa Postal 510, BR-18618970 Botucatu, SP, BrazilInst Medicina Tropical Sao PauloCtr Paula SouzaUniversidade Estadual Paulista (Unesp)Fac Integradas BauruUniv Sagrad CoracaoTrevisani Correa, Claudia RosanaOliveira-Arbex, Ana Paula [UNESP]David, Erica Boarato [UNESP]Guimaraes, Semiramis [UNESP]2020-12-10T19:54:55Z2020-12-10T19:54:55Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.1590/S1678-9946202062020Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 62, 10 p., 2020.0036-4665http://hdl.handle.net/11449/19674610.1590/S1678-9946202062020S0036-46652020000100212WOS:000522849300002S0036-46652020000100212.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Do Instituto De Medicina Tropical De Sao Pauloinfo:eu-repo/semantics/openAccess2023-10-17T06:10:44Zoai:repositorio.unesp.br:11449/196746Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:12:37.375882Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic analysis of Giardia duodenalis isolates from children of low-income families living in an economically successful region in Southeastern Brazil |
title |
Genetic analysis of Giardia duodenalis isolates from children of low-income families living in an economically successful region in Southeastern Brazil |
spellingShingle |
Genetic analysis of Giardia duodenalis isolates from children of low-income families living in an economically successful region in Southeastern Brazil Trevisani Correa, Claudia Rosana Giardia duodenalis Genetic diversity Children Daycare Low income |
title_short |
Genetic analysis of Giardia duodenalis isolates from children of low-income families living in an economically successful region in Southeastern Brazil |
title_full |
Genetic analysis of Giardia duodenalis isolates from children of low-income families living in an economically successful region in Southeastern Brazil |
title_fullStr |
Genetic analysis of Giardia duodenalis isolates from children of low-income families living in an economically successful region in Southeastern Brazil |
title_full_unstemmed |
Genetic analysis of Giardia duodenalis isolates from children of low-income families living in an economically successful region in Southeastern Brazil |
title_sort |
Genetic analysis of Giardia duodenalis isolates from children of low-income families living in an economically successful region in Southeastern Brazil |
author |
Trevisani Correa, Claudia Rosana |
author_facet |
Trevisani Correa, Claudia Rosana Oliveira-Arbex, Ana Paula [UNESP] David, Erica Boarato [UNESP] Guimaraes, Semiramis [UNESP] |
author_role |
author |
author2 |
Oliveira-Arbex, Ana Paula [UNESP] David, Erica Boarato [UNESP] Guimaraes, Semiramis [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Ctr Paula Souza Universidade Estadual Paulista (Unesp) Fac Integradas Bauru Univ Sagrad Coracao |
dc.contributor.author.fl_str_mv |
Trevisani Correa, Claudia Rosana Oliveira-Arbex, Ana Paula [UNESP] David, Erica Boarato [UNESP] Guimaraes, Semiramis [UNESP] |
dc.subject.por.fl_str_mv |
Giardia duodenalis Genetic diversity Children Daycare Low income |
topic |
Giardia duodenalis Genetic diversity Children Daycare Low income |
description |
Giardia duodenalis is one of the most important and widespread gastrointestinal parasites in the world. Despite its relevance as a causative agent of diarrhea, asymptomatic giardiasis occurs frequently. especially in low resources settings in which children are exposed to many risk factors. Based on microscopic examination and the polymerase chain reaction (PCR) amplification and sequencing of beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes, we assessed G. duodenalis occurrence and genetic diversity in isolates of children attending a daycare center and living in low income families, in an economically successful region. Considering both, microscopic examination and PCR/sequencing methods, the overall prevalence of Giardia infection was 51.4%, with the highest frequency in children aged 1-4 years old (p<0.05). Genotyping of 50 isolates revealed that the assemblage A was found in 60% of the samples (30/50), followed by the assemblage B in 38% (19/50) and 2% of mixed-assemblage infections (1/50). At the sub-assemblage level, isolates genotyped as A were All and among isolates B, BIII and BIV were identified. Both assemblages A and B were detected in children of all age groups . however assemblage A was more prevalent. The detection of anthroponotic assemblages and sub-assemblages (AII, BM and BIV) reinforces human-to-human transmission, mainly in children of all age groups when they have not yet received toilet training, making them more vulnerable to infection. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T19:54:55Z 2020-12-10T19:54:55Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-9946202062020 Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 62, 10 p., 2020. 0036-4665 http://hdl.handle.net/11449/196746 10.1590/S1678-9946202062020 S0036-46652020000100212 WOS:000522849300002 S0036-46652020000100212.pdf |
url |
http://dx.doi.org/10.1590/S1678-9946202062020 http://hdl.handle.net/11449/196746 |
identifier_str_mv |
Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 62, 10 p., 2020. 0036-4665 10.1590/S1678-9946202062020 S0036-46652020000100212 WOS:000522849300002 S0036-46652020000100212.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Revista Do Instituto De Medicina Tropical De Sao Paulo |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.publisher.none.fl_str_mv |
Inst Medicina Tropical Sao Paulo |
publisher.none.fl_str_mv |
Inst Medicina Tropical Sao Paulo |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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