Ligninase and Cellulase Activity of Lentinula edodes (Berk.) Pegler Strains in Different Culture Media
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/218869 |
Resumo: | This study was conducted with the objective of evaluating the growth of six strains of Lentinula edodes (LE1, LE2, LE3, LE4, LE5 and LE6) in five culture media and characterizes strains for enzyme production. The culture medium that provided the best mycelial growth of the strains was used for semi-quantitative tests of ligninase and cellulase activity. Samples for enzyme assays and determination of total proteins were collected at 7, 14, 21 and 28 days of incubation to building an enzymatic curve. The strain LE5 is the most aggressive, with higher mycelial growth rate and mycelial mass production in the basic medium added of 0.01% yeast extract. The addition of biotin and thiamine to the culture medium is not sufficient to substitute yeast extract. The performance of the strains with respect to the production of cellulase and ligninase was different. The LE6 strain excelled in the production of laccase, manganese peroxidase and beta-glucosidase with 28 days of incubation and endo-beta-1,4-glucanase with 14 days of incubation, while strain LE5 excelled in the production of exo-beta-1,4-glucanase with 7 days of incubation. |
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Ligninase and Cellulase Activity of Lentinula edodes (Berk.) Pegler Strains in Different Culture MediaThis study was conducted with the objective of evaluating the growth of six strains of Lentinula edodes (LE1, LE2, LE3, LE4, LE5 and LE6) in five culture media and characterizes strains for enzyme production. The culture medium that provided the best mycelial growth of the strains was used for semi-quantitative tests of ligninase and cellulase activity. Samples for enzyme assays and determination of total proteins were collected at 7, 14, 21 and 28 days of incubation to building an enzymatic curve. The strain LE5 is the most aggressive, with higher mycelial growth rate and mycelial mass production in the basic medium added of 0.01% yeast extract. The addition of biotin and thiamine to the culture medium is not sufficient to substitute yeast extract. The performance of the strains with respect to the production of cellulase and ligninase was different. The LE6 strain excelled in the production of laccase, manganese peroxidase and beta-glucosidase with 28 days of incubation and endo-beta-1,4-glucanase with 14 days of incubation, while strain LE5 excelled in the production of exo-beta-1,4-glucanase with 7 days of incubation.Univ Lavras, Dept Biol UFLA, POB 3037, BR-37200000 Lavras, MG, BrazilUniv Lavras, Dept Food Sci UFLA, POB 3037, BR-37200000 Lavras, MG, BrazilSao Paulo State Univ UNESP, Sch Agr Sci & Technol FCAT, BR-17900000 Dracena, SP, BrazilSao Paulo State Univ UNESP, Sch Agr Sci & Technol FCAT, BR-17900000 Dracena, SP, BrazilDr M N KhanUniversidade Federal de Lavras (UFLA)Universidade Estadual Paulista (UNESP)Carvalho, M. A.Costa, L. M. A. S.Santos, D. M. da Silveira E.Dias, D. R.Zied, D. C. [UNESP]Dias, E. S.2022-04-28T17:30:18Z2022-04-28T17:30:18Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1683-1691Journal Of Pure And Applied Microbiology. Bhopal: Dr M N Khan, v. 10, n. 3, p. 1683-1691, 2016.0973-7510http://hdl.handle.net/11449/218869WOS:000386421600002Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Pure And Applied Microbiologyinfo:eu-repo/semantics/openAccess2024-05-07T13:47:22Zoai:repositorio.unesp.br:11449/218869Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-05-07T13:47:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ligninase and Cellulase Activity of Lentinula edodes (Berk.) Pegler Strains in Different Culture Media |
title |
Ligninase and Cellulase Activity of Lentinula edodes (Berk.) Pegler Strains in Different Culture Media |
spellingShingle |
Ligninase and Cellulase Activity of Lentinula edodes (Berk.) Pegler Strains in Different Culture Media Carvalho, M. A. |
title_short |
Ligninase and Cellulase Activity of Lentinula edodes (Berk.) Pegler Strains in Different Culture Media |
title_full |
Ligninase and Cellulase Activity of Lentinula edodes (Berk.) Pegler Strains in Different Culture Media |
title_fullStr |
Ligninase and Cellulase Activity of Lentinula edodes (Berk.) Pegler Strains in Different Culture Media |
title_full_unstemmed |
Ligninase and Cellulase Activity of Lentinula edodes (Berk.) Pegler Strains in Different Culture Media |
title_sort |
Ligninase and Cellulase Activity of Lentinula edodes (Berk.) Pegler Strains in Different Culture Media |
author |
Carvalho, M. A. |
author_facet |
Carvalho, M. A. Costa, L. M. A. S. Santos, D. M. da Silveira E. Dias, D. R. Zied, D. C. [UNESP] Dias, E. S. |
author_role |
author |
author2 |
Costa, L. M. A. S. Santos, D. M. da Silveira E. Dias, D. R. Zied, D. C. [UNESP] Dias, E. S. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Lavras (UFLA) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Carvalho, M. A. Costa, L. M. A. S. Santos, D. M. da Silveira E. Dias, D. R. Zied, D. C. [UNESP] Dias, E. S. |
description |
This study was conducted with the objective of evaluating the growth of six strains of Lentinula edodes (LE1, LE2, LE3, LE4, LE5 and LE6) in five culture media and characterizes strains for enzyme production. The culture medium that provided the best mycelial growth of the strains was used for semi-quantitative tests of ligninase and cellulase activity. Samples for enzyme assays and determination of total proteins were collected at 7, 14, 21 and 28 days of incubation to building an enzymatic curve. The strain LE5 is the most aggressive, with higher mycelial growth rate and mycelial mass production in the basic medium added of 0.01% yeast extract. The addition of biotin and thiamine to the culture medium is not sufficient to substitute yeast extract. The performance of the strains with respect to the production of cellulase and ligninase was different. The LE6 strain excelled in the production of laccase, manganese peroxidase and beta-glucosidase with 28 days of incubation and endo-beta-1,4-glucanase with 14 days of incubation, while strain LE5 excelled in the production of exo-beta-1,4-glucanase with 7 days of incubation. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 2022-04-28T17:30:18Z 2022-04-28T17:30:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal Of Pure And Applied Microbiology. Bhopal: Dr M N Khan, v. 10, n. 3, p. 1683-1691, 2016. 0973-7510 http://hdl.handle.net/11449/218869 WOS:000386421600002 |
identifier_str_mv |
Journal Of Pure And Applied Microbiology. Bhopal: Dr M N Khan, v. 10, n. 3, p. 1683-1691, 2016. 0973-7510 WOS:000386421600002 |
url |
http://hdl.handle.net/11449/218869 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Pure And Applied Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1683-1691 |
dc.publisher.none.fl_str_mv |
Dr M N Khan |
publisher.none.fl_str_mv |
Dr M N Khan |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964915196231680 |