Progesterone levels and days to luteolysis in mares treated with intrauterine fractionated coconut oil

Detalhes bibliográficos
Autor(a) principal: Diel de Amorim, Mariana
Data de Publicação: 2016
Outros Autores: Nielsen, Kayla, Cruz, Raissa Karolliny Salgueiro [UNESP], Card, Claire
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.theriogenology.2016.02.002
http://hdl.handle.net/11449/168445
Resumo: Intrauterine plant oil infusion, including fractionated coconut oil, has been previously found to be a safe, inexpensive, and reversible method of prolonging the luteal phase in mares when administered on Day 10 of the estrous cycle. Our objective was to understand the uteroovarian response to the administration of fractionated coconut oil infusion in the uterus of diestrous mares. We hypothesized that intrauterine coconut oil administration on Day 10 would prolong luteal life span in a dose-dependent fashion and would result in higher serum progesterone levels than untreated mares at the expected time of luteolysis. Light-horse mares (n = 18) were examined using transrectal palpation and ultrasonography to determine if they had a normal interovulatory interval and were then examined daily in estrus until the day of ovulation (Day 0) and then every other day during an estrous cycle. Jugular blood was drawn on Day 11, Day 13, Day 15, and Day 17, centrifuged, and serum stored until assayed for progesterone (P4; Siemens Coat-a-Count Progesterone RIA, Los Angeles, CA, USA). Mares were randomly assigned to treatment and studied over one to two estrous cycles with a rest cycle after each treatment cycle. Groups were: control (n = 5), fractionated coconut oil 1.0 mL (Miglyol 810; Sasol Oil, Witten, Germany) infused in the uterus with an artificial insemination pipette on Day 10 (Group 1; n = 5) and fractionated coconut oil 0.5 mL infused in the uterus with an embryo transfer gun, on Day 10 (Group 2; n = 5). All statistical analyses were performed using analytical software (Stata SE, version 13.1, College Station, TX, USA) at P < 0.05. Data were first evaluated using the Shapiro–Wilk test for normality. Differences between groups in days to luteolysis (DTL) were examined using analysis of variance and Bonferroni, and the effect of day and treatment on P4 levels were examined using the Kruskal–Wallis and Dunn's all pairwise test. There was a significant difference in DTL between the groups (P = 0.0083), with fewer DTL in Group 1 compared to control (P = 0.011) and to Group 2 (P = 0.034). There was a significant effect of day (P < 0.0001) on P4 levels with Day 11 P4 levels higher than Day 15 and Day 17 and Day 13 P4 levels higher than Day 15 or Day 17 (P < 0.03). There was a significant effect of treatment group (P = 0.0098) on P4 levels with control and Group 2 levels higher than Group 1 mares (P = 0.0012, P = 0.0495, respectively). We concluded that intrauterine administration of 1 mL of fractionated coconut oil lowered P4 levels in diestrus in a dose-dependent fashion and did not prolonged the luteal phase of the mares.
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spelling Progesterone levels and days to luteolysis in mares treated with intrauterine fractionated coconut oilCoconut oilDays to luteolysisMareProgesteroneIntrauterine plant oil infusion, including fractionated coconut oil, has been previously found to be a safe, inexpensive, and reversible method of prolonging the luteal phase in mares when administered on Day 10 of the estrous cycle. Our objective was to understand the uteroovarian response to the administration of fractionated coconut oil infusion in the uterus of diestrous mares. We hypothesized that intrauterine coconut oil administration on Day 10 would prolong luteal life span in a dose-dependent fashion and would result in higher serum progesterone levels than untreated mares at the expected time of luteolysis. Light-horse mares (n = 18) were examined using transrectal palpation and ultrasonography to determine if they had a normal interovulatory interval and were then examined daily in estrus until the day of ovulation (Day 0) and then every other day during an estrous cycle. Jugular blood was drawn on Day 11, Day 13, Day 15, and Day 17, centrifuged, and serum stored until assayed for progesterone (P4; Siemens Coat-a-Count Progesterone RIA, Los Angeles, CA, USA). Mares were randomly assigned to treatment and studied over one to two estrous cycles with a rest cycle after each treatment cycle. Groups were: control (n = 5), fractionated coconut oil 1.0 mL (Miglyol 810; Sasol Oil, Witten, Germany) infused in the uterus with an artificial insemination pipette on Day 10 (Group 1; n = 5) and fractionated coconut oil 0.5 mL infused in the uterus with an embryo transfer gun, on Day 10 (Group 2; n = 5). All statistical analyses were performed using analytical software (Stata SE, version 13.1, College Station, TX, USA) at P < 0.05. Data were first evaluated using the Shapiro–Wilk test for normality. Differences between groups in days to luteolysis (DTL) were examined using analysis of variance and Bonferroni, and the effect of day and treatment on P4 levels were examined using the Kruskal–Wallis and Dunn's all pairwise test. There was a significant difference in DTL between the groups (P = 0.0083), with fewer DTL in Group 1 compared to control (P = 0.011) and to Group 2 (P = 0.034). There was a significant effect of day (P < 0.0001) on P4 levels with Day 11 P4 levels higher than Day 15 and Day 17 and Day 13 P4 levels higher than Day 15 or Day 17 (P < 0.03). There was a significant effect of treatment group (P = 0.0098) on P4 levels with control and Group 2 levels higher than Group 1 mares (P = 0.0012, P = 0.0495, respectively). We concluded that intrauterine administration of 1 mL of fractionated coconut oil lowered P4 levels in diestrus in a dose-dependent fashion and did not prolonged the luteal phase of the mares.University of SaskatchewanDepartment of Large Animal Clinical Sciences Western College of Veterinary Medicine University of SaskatchewanDepartment of Veterinary Science School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)Department of Health Management Atlantic Veterinary College University of Prince Edward Island, 550 University AvenueDepartment of Veterinary Science School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)University of Saskatchewan: 414568University of SaskatchewanUniversidade Estadual Paulista (Unesp)University of Prince Edward IslandDiel de Amorim, MarianaNielsen, KaylaCruz, Raissa Karolliny Salgueiro [UNESP]Card, Claire2018-12-11T16:41:18Z2018-12-11T16:41:18Z2016-01-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject545-550application/pdfhttp://dx.doi.org/10.1016/j.theriogenology.2016.02.002Theriogenology, v. 86, n. 2, p. 545-550, 2016.0093-691Xhttp://hdl.handle.net/11449/16844510.1016/j.theriogenology.2016.02.0022-s2.0-849594759332-s2.0-84959475933.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2023-11-23T06:10:41Zoai:repositorio.unesp.br:11449/168445Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:28:59.979673Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Progesterone levels and days to luteolysis in mares treated with intrauterine fractionated coconut oil
title Progesterone levels and days to luteolysis in mares treated with intrauterine fractionated coconut oil
spellingShingle Progesterone levels and days to luteolysis in mares treated with intrauterine fractionated coconut oil
Diel de Amorim, Mariana
Coconut oil
Days to luteolysis
Mare
Progesterone
title_short Progesterone levels and days to luteolysis in mares treated with intrauterine fractionated coconut oil
title_full Progesterone levels and days to luteolysis in mares treated with intrauterine fractionated coconut oil
title_fullStr Progesterone levels and days to luteolysis in mares treated with intrauterine fractionated coconut oil
title_full_unstemmed Progesterone levels and days to luteolysis in mares treated with intrauterine fractionated coconut oil
title_sort Progesterone levels and days to luteolysis in mares treated with intrauterine fractionated coconut oil
author Diel de Amorim, Mariana
author_facet Diel de Amorim, Mariana
Nielsen, Kayla
Cruz, Raissa Karolliny Salgueiro [UNESP]
Card, Claire
author_role author
author2 Nielsen, Kayla
Cruz, Raissa Karolliny Salgueiro [UNESP]
Card, Claire
author2_role author
author
author
dc.contributor.none.fl_str_mv University of Saskatchewan
Universidade Estadual Paulista (Unesp)
University of Prince Edward Island
dc.contributor.author.fl_str_mv Diel de Amorim, Mariana
Nielsen, Kayla
Cruz, Raissa Karolliny Salgueiro [UNESP]
Card, Claire
dc.subject.por.fl_str_mv Coconut oil
Days to luteolysis
Mare
Progesterone
topic Coconut oil
Days to luteolysis
Mare
Progesterone
description Intrauterine plant oil infusion, including fractionated coconut oil, has been previously found to be a safe, inexpensive, and reversible method of prolonging the luteal phase in mares when administered on Day 10 of the estrous cycle. Our objective was to understand the uteroovarian response to the administration of fractionated coconut oil infusion in the uterus of diestrous mares. We hypothesized that intrauterine coconut oil administration on Day 10 would prolong luteal life span in a dose-dependent fashion and would result in higher serum progesterone levels than untreated mares at the expected time of luteolysis. Light-horse mares (n = 18) were examined using transrectal palpation and ultrasonography to determine if they had a normal interovulatory interval and were then examined daily in estrus until the day of ovulation (Day 0) and then every other day during an estrous cycle. Jugular blood was drawn on Day 11, Day 13, Day 15, and Day 17, centrifuged, and serum stored until assayed for progesterone (P4; Siemens Coat-a-Count Progesterone RIA, Los Angeles, CA, USA). Mares were randomly assigned to treatment and studied over one to two estrous cycles with a rest cycle after each treatment cycle. Groups were: control (n = 5), fractionated coconut oil 1.0 mL (Miglyol 810; Sasol Oil, Witten, Germany) infused in the uterus with an artificial insemination pipette on Day 10 (Group 1; n = 5) and fractionated coconut oil 0.5 mL infused in the uterus with an embryo transfer gun, on Day 10 (Group 2; n = 5). All statistical analyses were performed using analytical software (Stata SE, version 13.1, College Station, TX, USA) at P < 0.05. Data were first evaluated using the Shapiro–Wilk test for normality. Differences between groups in days to luteolysis (DTL) were examined using analysis of variance and Bonferroni, and the effect of day and treatment on P4 levels were examined using the Kruskal–Wallis and Dunn's all pairwise test. There was a significant difference in DTL between the groups (P = 0.0083), with fewer DTL in Group 1 compared to control (P = 0.011) and to Group 2 (P = 0.034). There was a significant effect of day (P < 0.0001) on P4 levels with Day 11 P4 levels higher than Day 15 and Day 17 and Day 13 P4 levels higher than Day 15 or Day 17 (P < 0.03). There was a significant effect of treatment group (P = 0.0098) on P4 levels with control and Group 2 levels higher than Group 1 mares (P = 0.0012, P = 0.0495, respectively). We concluded that intrauterine administration of 1 mL of fractionated coconut oil lowered P4 levels in diestrus in a dose-dependent fashion and did not prolonged the luteal phase of the mares.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-14
2018-12-11T16:41:18Z
2018-12-11T16:41:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.theriogenology.2016.02.002
Theriogenology, v. 86, n. 2, p. 545-550, 2016.
0093-691X
http://hdl.handle.net/11449/168445
10.1016/j.theriogenology.2016.02.002
2-s2.0-84959475933
2-s2.0-84959475933.pdf
url http://dx.doi.org/10.1016/j.theriogenology.2016.02.002
http://hdl.handle.net/11449/168445
identifier_str_mv Theriogenology, v. 86, n. 2, p. 545-550, 2016.
0093-691X
10.1016/j.theriogenology.2016.02.002
2-s2.0-84959475933
2-s2.0-84959475933.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Theriogenology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 545-550
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
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instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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