Ultrastructure of two microsporidians Inodosporus sp. and Myospora sp. co-infecting muscles of the Amazon River prawn Macrobrachium amazonicum (Heller, 1862)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/2358-2936e2021046 http://hdl.handle.net/11449/218831 |
Resumo: | In the present study, we describe a co-infection of two microsporidians within the abdominal muscle of the Amazon River prawnMacrobrachium amazonicum from Brazil, detected through light and transmission electron microscopy and histopathological analysis. Two spore types and respective early developmental stages (meronts, sporonts, and sporoblasts) appeared grouped in numerous adjacent clusters among the muscle fibers of the host. Spores were initially divided into two morphotypes, Sp. 1 and Sp. 2, based on differences in shape, size, and internal organization. The Sp. 1 spores are pyriform to ovoid (4.3 +/- 0.3 x 3.7 +/- 0.4 mu m in size) and resided in groups of eight within a sporophorous vesicle. Sp. 1 spores had bilayered walls and long tape-like external filaments with irregular morphology and size, forming a complex contiguous membranous system attached to the spore wall. The umbrella-like anchoring disc of the spores was in continuity with an anisofilar polar tube arranged in 7-8 (rarely 9) coils. The Sp. 2 spores were rod-like in shape (3.1 +/- 0.5 x 0.8 +/- 0.1 mu m in diameter) and the polar tube had 7-8 coils. Their merogonic and sporogonic stages occurred within the sarcoplasm of the muscle cells in close contact with the myofibrils. Based on ultrastructural organization of the sporogonic stages, the Sp. 1 and Sp. 2 morphotypes probably belong to the genera Inodosporus and Myospora, respectively. |
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Ultrastructure of two microsporidians Inodosporus sp. and Myospora sp. co-infecting muscles of the Amazon River prawn Macrobrachium amazonicum (Heller, 1862)CrustaceaMicrosporidiamuscle infectionspore dimorphismIn the present study, we describe a co-infection of two microsporidians within the abdominal muscle of the Amazon River prawnMacrobrachium amazonicum from Brazil, detected through light and transmission electron microscopy and histopathological analysis. Two spore types and respective early developmental stages (meronts, sporonts, and sporoblasts) appeared grouped in numerous adjacent clusters among the muscle fibers of the host. Spores were initially divided into two morphotypes, Sp. 1 and Sp. 2, based on differences in shape, size, and internal organization. The Sp. 1 spores are pyriform to ovoid (4.3 +/- 0.3 x 3.7 +/- 0.4 mu m in size) and resided in groups of eight within a sporophorous vesicle. Sp. 1 spores had bilayered walls and long tape-like external filaments with irregular morphology and size, forming a complex contiguous membranous system attached to the spore wall. The umbrella-like anchoring disc of the spores was in continuity with an anisofilar polar tube arranged in 7-8 (rarely 9) coils. The Sp. 2 spores were rod-like in shape (3.1 +/- 0.5 x 0.8 +/- 0.1 mu m in diameter) and the polar tube had 7-8 coils. Their merogonic and sporogonic stages occurred within the sarcoplasm of the muscle cells in close contact with the myofibrils. Based on ultrastructural organization of the sporogonic stages, the Sp. 1 and Sp. 2 morphotypes probably belong to the genera Inodosporus and Myospora, respectively.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista UNESP, Programa Posgrad Ciencias Biol Zool, Inst Biociencias, BR-13506900 Rio Claro, SP, BrazilFIT MG, Fac Inovacao & Tecnol Minas Gerais, BR-37900060 Passos, MG, BrazilUniv Estadual Paulista UNESP, Lab Morfol Invertebrados LMI, Dept Biol, Fac Ciencias Agr & Ve Jaboticabal, BR-14884900 Jaboticabal, SP, BrazilUniv Estadual Paulista UNESP, Ctr Aquicultura CAUNESP, Fac Ciencias Agr & Ve Jaboticabal, BR-14884900 Jaboticabal, SP, BrazilUniv Porto, Inst Invest & Inovacao Saude I3S, P-4200135 Porto, PortugalUniv Porto ICBAS UP, Lab Cell Biol, Inst Biomed Sci, P-4050313 Porto, PortugalUniv Inst Hlth Sci TOXRUN, Toxicol Res Unit, CESPU, P-4585116 Gandra, PortugalUniv Porto, Lab Anim Pathol, Interdisciplinary Ctr Marine & Environm Res CIIMA, P-4450208 Matosinhos, PortugalUniv Estadual Paulista UNESP, Programa Posgrad Ciencias Biol Zool, Inst Biociencias, BR-13506900 Rio Claro, SP, BrazilUniv Estadual Paulista UNESP, Lab Morfol Invertebrados LMI, Dept Biol, Fac Ciencias Agr & Ve Jaboticabal, BR-14884900 Jaboticabal, SP, BrazilUniv Estadual Paulista UNESP, Ctr Aquicultura CAUNESP, Fac Ciencias Agr & Ve Jaboticabal, BR-14884900 Jaboticabal, SP, BrazilCAPES: 001CAPES: 1989/2014CAPES: 23038.004309/2014-5FAPESP: JP 2005#04707-5FAPESP: 2018/13685-5CNPq: 309298/2020-2Soc Brasileira CarcinologiaUniversidade Estadual Paulista (UNESP)FIT MGUniv PortoUniv Porto ICBAS UPUniv Inst Hlth Sci TOXRUNPaschoal, Lucas R. P. [UNESP]Zara, Fernando J. [UNESP]Rocha, SoniaAlves, AngelaCasal, GracaAzevedo, Carlos2022-04-28T17:23:17Z2022-04-28T17:23:17Z2021-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13http://dx.doi.org/10.1590/2358-2936e2021046Nauplius. Rio Grande Rs: Soc Brasileira Carcinologia, v. 29, 13 p., 2021.0104-6497http://hdl.handle.net/11449/21883110.1590/2358-2936e2021046WOS:000763607300001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNaupliusinfo:eu-repo/semantics/openAccess2022-04-28T17:23:17Zoai:repositorio.unesp.br:11449/218831Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:00:16.151823Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ultrastructure of two microsporidians Inodosporus sp. and Myospora sp. co-infecting muscles of the Amazon River prawn Macrobrachium amazonicum (Heller, 1862) |
title |
Ultrastructure of two microsporidians Inodosporus sp. and Myospora sp. co-infecting muscles of the Amazon River prawn Macrobrachium amazonicum (Heller, 1862) |
spellingShingle |
Ultrastructure of two microsporidians Inodosporus sp. and Myospora sp. co-infecting muscles of the Amazon River prawn Macrobrachium amazonicum (Heller, 1862) Paschoal, Lucas R. P. [UNESP] Crustacea Microsporidia muscle infection spore dimorphism |
title_short |
Ultrastructure of two microsporidians Inodosporus sp. and Myospora sp. co-infecting muscles of the Amazon River prawn Macrobrachium amazonicum (Heller, 1862) |
title_full |
Ultrastructure of two microsporidians Inodosporus sp. and Myospora sp. co-infecting muscles of the Amazon River prawn Macrobrachium amazonicum (Heller, 1862) |
title_fullStr |
Ultrastructure of two microsporidians Inodosporus sp. and Myospora sp. co-infecting muscles of the Amazon River prawn Macrobrachium amazonicum (Heller, 1862) |
title_full_unstemmed |
Ultrastructure of two microsporidians Inodosporus sp. and Myospora sp. co-infecting muscles of the Amazon River prawn Macrobrachium amazonicum (Heller, 1862) |
title_sort |
Ultrastructure of two microsporidians Inodosporus sp. and Myospora sp. co-infecting muscles of the Amazon River prawn Macrobrachium amazonicum (Heller, 1862) |
author |
Paschoal, Lucas R. P. [UNESP] |
author_facet |
Paschoal, Lucas R. P. [UNESP] Zara, Fernando J. [UNESP] Rocha, Sonia Alves, Angela Casal, Graca Azevedo, Carlos |
author_role |
author |
author2 |
Zara, Fernando J. [UNESP] Rocha, Sonia Alves, Angela Casal, Graca Azevedo, Carlos |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) FIT MG Univ Porto Univ Porto ICBAS UP Univ Inst Hlth Sci TOXRUN |
dc.contributor.author.fl_str_mv |
Paschoal, Lucas R. P. [UNESP] Zara, Fernando J. [UNESP] Rocha, Sonia Alves, Angela Casal, Graca Azevedo, Carlos |
dc.subject.por.fl_str_mv |
Crustacea Microsporidia muscle infection spore dimorphism |
topic |
Crustacea Microsporidia muscle infection spore dimorphism |
description |
In the present study, we describe a co-infection of two microsporidians within the abdominal muscle of the Amazon River prawnMacrobrachium amazonicum from Brazil, detected through light and transmission electron microscopy and histopathological analysis. Two spore types and respective early developmental stages (meronts, sporonts, and sporoblasts) appeared grouped in numerous adjacent clusters among the muscle fibers of the host. Spores were initially divided into two morphotypes, Sp. 1 and Sp. 2, based on differences in shape, size, and internal organization. The Sp. 1 spores are pyriform to ovoid (4.3 +/- 0.3 x 3.7 +/- 0.4 mu m in size) and resided in groups of eight within a sporophorous vesicle. Sp. 1 spores had bilayered walls and long tape-like external filaments with irregular morphology and size, forming a complex contiguous membranous system attached to the spore wall. The umbrella-like anchoring disc of the spores was in continuity with an anisofilar polar tube arranged in 7-8 (rarely 9) coils. The Sp. 2 spores were rod-like in shape (3.1 +/- 0.5 x 0.8 +/- 0.1 mu m in diameter) and the polar tube had 7-8 coils. Their merogonic and sporogonic stages occurred within the sarcoplasm of the muscle cells in close contact with the myofibrils. Based on ultrastructural organization of the sporogonic stages, the Sp. 1 and Sp. 2 morphotypes probably belong to the genera Inodosporus and Myospora, respectively. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-15 2022-04-28T17:23:17Z 2022-04-28T17:23:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/2358-2936e2021046 Nauplius. Rio Grande Rs: Soc Brasileira Carcinologia, v. 29, 13 p., 2021. 0104-6497 http://hdl.handle.net/11449/218831 10.1590/2358-2936e2021046 WOS:000763607300001 |
url |
http://dx.doi.org/10.1590/2358-2936e2021046 http://hdl.handle.net/11449/218831 |
identifier_str_mv |
Nauplius. Rio Grande Rs: Soc Brasileira Carcinologia, v. 29, 13 p., 2021. 0104-6497 10.1590/2358-2936e2021046 WOS:000763607300001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Nauplius |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
13 |
dc.publisher.none.fl_str_mv |
Soc Brasileira Carcinologia |
publisher.none.fl_str_mv |
Soc Brasileira Carcinologia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128883425280000 |