Utilização de meios alternativos e diferentes tipos de recipientes no cultivo de Ankistrodesmus gracilis (Reinsch) Korshikov (Chlorophyceae) em laboratório

• Autor(a) principal: Sipauba-Tavares, Lucia Helena [UNESP]
• Data de Publicação: 2011
• Outros Autores: Millan, Rodrigo Ney [UNESP], Berchielli, Flávia de Almeida [UNESP], de Souza Braga, Francisco Manoel [UNESP]
• Tipo de documento: Artigo
• Idioma: eng; por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.4025/actascibiolsci.v33i3.8046; http://hdl.handle.net/11449/72593
• topic: Algae; Biochemical composition; CHU 12; Growth; Macrophyte medium; NPK; ammonia; glass fiber; lipid; nitrate; phosphate; phosphorus; plastic; algal cell culture; algal growth; carboy; cell density; chemical composition; Chlorophyceae; CHU12; container; controlled study; culture medium; Eichhornia; growth curve; growth rate; laboratory; macrophyte; microbiology; nonhuman; protein content; algae; Ankistrodesmus gracilis; Eichhornia crassipes

A laboratory culture of Ankistrodesmus gracilis algae was evaluated by studying the biology of the species and its chemical composition in a traditional medium (CHU 12) and in two alternative culture media, NPK (20-5-20) and macrophyte (Eichhornia crassipes) + NPK, in three different types of recipients (fiberglass, carboy and plastic bag). First peak in the growth curve of Ankistrodesmus gracilis occurred on the ninth day in macrophyte + NPK medium (74.16 x 10 5 cells mL -1) in a fiberglass recipient. However, highest density (p < 0.01) was reported in medium CHU 12 (122.87 x 10 5 cells mL -1) in a plastic bag on the twelfth day. Cell density was over 70 x 10 5 cells mL -1 starting on the twelfth day. Growth rate of A. gracilis was similar (p > 0.05) in culture media in the three recipients. Protein and fiber were similar (p > 0.05) in the treatments, but lipids were higher (p < 0.05) in NPK. Nitrate, ammonia, total phosphorus and orthophosphate contents were over 1 mg L -1 in NPK (p < 0.01). Results show that alternative media, such as NPK and macrophyte + NPK, are possible for large-scale culture of A. gracilis cultured in three types of recipients. Costs are low, occupying less space when cultured in plastic bags and in the laboratory.